Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4259 and Pf6N2E2_4260 are separated by 11 nucleotides Pf6N2E2_4260 and Pf6N2E2_4261 overlap by 13 nucleotides Pf6N2E2_4261 and Pf6N2E2_4262 overlap by 4 nucleotides
Pf6N2E2_4259 - Zinc ABC transporter, periplasmic-binding protein ZnuA, at 4,957,442 to 4,958,317
_4259
Pf6N2E2_4260 - Zinc ABC transporter, inner membrane permease protein ZnuB, at 4,958,329 to 4,959,207
_4260
Pf6N2E2_4261 - Zinc ABC transporter, ATP-binding protein ZnuC, at 4,959,195 to 4,959,863
_4261
Pf6N2E2_4262 - GTP cyclohydrolase I (EC 3.5.4.16) type 2, at 4,959,860 to 4,960,684
_4262
Position (kb)
4959
4960 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4958.231 kb on - strand at 4958.231 kb on - strand at 4958.298 kb on - strand at 4958.309 kb on + strand at 4958.309 kb on + strand at 4958.309 kb on + strand at 4958.309 kb on + strand at 4958.310 kb on - strand at 4958.399 kb on + strand at 4958.399 kb on + strand at 4958.399 kb on + strand at 4958.399 kb on + strand at 4958.400 kb on - strand at 4958.447 kb on + strand, within Pf6N2E2_4260 at 4958.447 kb on + strand, within Pf6N2E2_4260 at 4958.447 kb on + strand, within Pf6N2E2_4260 at 4958.448 kb on - strand, within Pf6N2E2_4260 at 4958.448 kb on - strand, within Pf6N2E2_4260 at 4958.448 kb on - strand, within Pf6N2E2_4260 at 4958.450 kb on + strand, within Pf6N2E2_4260 at 4958.450 kb on + strand, within Pf6N2E2_4260 at 4958.450 kb on + strand, within Pf6N2E2_4260 at 4958.450 kb on + strand, within Pf6N2E2_4260 at 4958.450 kb on + strand, within Pf6N2E2_4260 at 4958.451 kb on - strand, within Pf6N2E2_4260 at 4958.451 kb on - strand, within Pf6N2E2_4260 at 4958.451 kb on - strand, within Pf6N2E2_4260 at 4958.451 kb on - strand, within Pf6N2E2_4260 at 4958.718 kb on - strand, within Pf6N2E2_4260 at 4958.879 kb on + strand, within Pf6N2E2_4260 at 4958.880 kb on - strand, within Pf6N2E2_4260 at 4958.880 kb on - strand, within Pf6N2E2_4260 at 4958.880 kb on - strand, within Pf6N2E2_4260 at 4958.908 kb on + strand, within Pf6N2E2_4260 at 4958.909 kb on - strand, within Pf6N2E2_4260 at 4958.909 kb on - strand, within Pf6N2E2_4260 at 4959.279 kb on - strand, within Pf6N2E2_4261 at 4959.279 kb on - strand, within Pf6N2E2_4261 at 4959.279 kb on - strand, within Pf6N2E2_4261 at 4959.279 kb on - strand, within Pf6N2E2_4261 at 4959.298 kb on - strand, within Pf6N2E2_4261 at 4959.535 kb on - strand, within Pf6N2E2_4261 at 4959.546 kb on + strand, within Pf6N2E2_4261 at 4959.546 kb on + strand, within Pf6N2E2_4261 at 4959.546 kb on + strand, within Pf6N2E2_4261 at 4959.546 kb on + strand, within Pf6N2E2_4261 at 4959.546 kb on + strand, within Pf6N2E2_4261 at 4959.547 kb on - strand, within Pf6N2E2_4261 at 4959.547 kb on - strand, within Pf6N2E2_4261 at 4959.547 kb on - strand, within Pf6N2E2_4261 at 4959.547 kb on - strand, within Pf6N2E2_4261 at 4959.547 kb on - strand, within Pf6N2E2_4261 at 4959.554 kb on + strand, within Pf6N2E2_4261 at 4959.554 kb on + strand, within Pf6N2E2_4261 at 4959.554 kb on + strand, within Pf6N2E2_4261 at 4959.554 kb on + strand, within Pf6N2E2_4261 at 4959.554 kb on + strand, within Pf6N2E2_4261 at 4959.554 kb on + strand, within Pf6N2E2_4261 at 4959.555 kb on - strand, within Pf6N2E2_4261 at 4959.735 kb on + strand, within Pf6N2E2_4261 at 4959.736 kb on - strand, within Pf6N2E2_4261 at 4959.736 kb on - strand, within Pf6N2E2_4261 at 4959.935 kb on + strand at 4959.935 kb on + strand at 4959.935 kb on + strand at 4959.935 kb on + strand at 4959.936 kb on - strand at 4959.936 kb on - strand at 4960.148 kb on + strand, within Pf6N2E2_4262 at 4960.148 kb on + strand, within Pf6N2E2_4262 at 4960.148 kb on + strand, within Pf6N2E2_4262 at 4960.149 kb on - strand, within Pf6N2E2_4262 at 4960.149 kb on - strand, within Pf6N2E2_4262 at 4960.149 kb on - strand, within Pf6N2E2_4262 at 4960.149 kb on - strand, within Pf6N2E2_4262 at 4960.149 kb on - strand, within Pf6N2E2_4262 at 4960.178 kb on - strand, within Pf6N2E2_4262 at 4960.249 kb on + strand, within Pf6N2E2_4262 at 4960.323 kb on + strand, within Pf6N2E2_4262 at 4960.324 kb on - strand, within Pf6N2E2_4262 at 4960.367 kb on + strand, within Pf6N2E2_4262 at 4960.367 kb on + strand, within Pf6N2E2_4262 at 4960.368 kb on - strand, within Pf6N2E2_4262 at 4960.442 kb on + strand, within Pf6N2E2_4262 at 4960.442 kb on + strand, within Pf6N2E2_4262 at 4960.442 kb on + strand, within Pf6N2E2_4262 at 4960.442 kb on + strand, within Pf6N2E2_4262 at 4960.442 kb on + strand, within Pf6N2E2_4262 at 4960.442 kb on + strand, within Pf6N2E2_4262 at 4960.442 kb on + strand, within Pf6N2E2_4262 at 4960.442 kb on + strand, within Pf6N2E2_4262 at 4960.443 kb on - strand, within Pf6N2E2_4262 at 4960.450 kb on + strand, within Pf6N2E2_4262 at 4960.451 kb on - strand, within Pf6N2E2_4262 at 4960.451 kb on - strand, within Pf6N2E2_4262 at 4960.479 kb on - strand, within Pf6N2E2_4262 at 4960.523 kb on + strand, within Pf6N2E2_4262 at 4960.523 kb on + strand, within Pf6N2E2_4262 at 4960.569 kb on + strand, within Pf6N2E2_4262 at 4960.569 kb on + strand, within Pf6N2E2_4262 at 4960.569 kb on + strand, within Pf6N2E2_4262 at 4960.570 kb on - strand, within Pf6N2E2_4262 at 4960.570 kb on - strand, within Pf6N2E2_4262 at 4960.571 kb on + strand, within Pf6N2E2_4262 at 4960.572 kb on - strand, within Pf6N2E2_4262 at 4960.572 kb on - strand, within Pf6N2E2_4262 at 4960.572 kb on - strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.582 kb on + strand, within Pf6N2E2_4262 at 4960.583 kb on - strand, within Pf6N2E2_4262 at 4960.583 kb on - strand, within Pf6N2E2_4262 at 4960.583 kb on - strand, within Pf6N2E2_4262 at 4960.583 kb on - strand, within Pf6N2E2_4262 at 4960.583 kb on - strand, within Pf6N2E2_4262 at 4960.583 kb on - strand, within Pf6N2E2_4262 at 4960.583 kb on - strand, within Pf6N2E2_4262 at 4960.583 kb on - strand, within Pf6N2E2_4262 at 4960.583 kb on - strand, within Pf6N2E2_4262 at 4960.583 kb on - strand, within Pf6N2E2_4262 at 4960.583 kb on - strand, within Pf6N2E2_4262 at 4960.583 kb on - strand, within Pf6N2E2_4262 at 4960.583 kb on - strand, within Pf6N2E2_4262
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,958,231 - +0.7 4,958,231 - +0.1 4,958,298 - +1.2 4,958,309 + +0.8 4,958,309 + +0.6 4,958,309 + -0.1 4,958,309 + +0.6 4,958,310 - +0.1 4,958,399 + +0.6 4,958,399 + +1.6 4,958,399 + -1.1 4,958,399 + +0.4 4,958,400 - +0.5 4,958,447 + Pf6N2E2_4260 0.13 +3.2 4,958,447 + Pf6N2E2_4260 0.13 +0.4 4,958,447 + Pf6N2E2_4260 0.13 +0.9 4,958,448 - Pf6N2E2_4260 0.14 +0.4 4,958,448 - Pf6N2E2_4260 0.14 -0.8 4,958,448 - Pf6N2E2_4260 0.14 +1.7 4,958,450 + Pf6N2E2_4260 0.14 +0.6 4,958,450 + Pf6N2E2_4260 0.14 -0.1 4,958,450 + Pf6N2E2_4260 0.14 +0.4 4,958,450 + Pf6N2E2_4260 0.14 +0.7 4,958,450 + Pf6N2E2_4260 0.14 +1.4 4,958,451 - Pf6N2E2_4260 0.14 +0.3 4,958,451 - Pf6N2E2_4260 0.14 -0.5 4,958,451 - Pf6N2E2_4260 0.14 -0.2 4,958,451 - Pf6N2E2_4260 0.14 +0.8 4,958,718 - Pf6N2E2_4260 0.44 -0.7 4,958,879 + Pf6N2E2_4260 0.63 +1.8 4,958,880 - Pf6N2E2_4260 0.63 +0.3 4,958,880 - Pf6N2E2_4260 0.63 -0.3 4,958,880 - Pf6N2E2_4260 0.63 -0.4 4,958,908 + Pf6N2E2_4260 0.66 -0.5 4,958,909 - Pf6N2E2_4260 0.66 -0.9 4,958,909 - Pf6N2E2_4260 0.66 +0.8 4,959,279 - Pf6N2E2_4261 0.13 +0.2 4,959,279 - Pf6N2E2_4261 0.13 +0.6 4,959,279 - Pf6N2E2_4261 0.13 -0.1 4,959,279 - Pf6N2E2_4261 0.13 +0.8 4,959,298 - Pf6N2E2_4261 0.15 +1.0 4,959,535 - Pf6N2E2_4261 0.51 +0.2 4,959,546 + Pf6N2E2_4261 0.52 +0.7 4,959,546 + Pf6N2E2_4261 0.52 +0.6 4,959,546 + Pf6N2E2_4261 0.52 -0.6 4,959,546 + Pf6N2E2_4261 0.52 -0.1 4,959,546 + Pf6N2E2_4261 0.52 -0.2 4,959,547 - Pf6N2E2_4261 0.53 -0.9 4,959,547 - Pf6N2E2_4261 0.53 -0.1 4,959,547 - Pf6N2E2_4261 0.53 -0.9 4,959,547 - Pf6N2E2_4261 0.53 +0.1 4,959,547 - Pf6N2E2_4261 0.53 +0.9 4,959,554 + Pf6N2E2_4261 0.54 +0.5 4,959,554 + Pf6N2E2_4261 0.54 +0.3 4,959,554 + Pf6N2E2_4261 0.54 +0.7 4,959,554 + Pf6N2E2_4261 0.54 +0.7 4,959,554 + Pf6N2E2_4261 0.54 -0.1 4,959,554 + Pf6N2E2_4261 0.54 -0.1 4,959,555 - Pf6N2E2_4261 0.54 +0.4 4,959,735 + Pf6N2E2_4261 0.81 +0.1 4,959,736 - Pf6N2E2_4261 0.81 +0.2 4,959,736 - Pf6N2E2_4261 0.81 +0.6 4,959,935 + +1.4 4,959,935 + -0.9 4,959,935 + +0.4 4,959,935 + -1.0 4,959,936 - +0.1 4,959,936 - +0.1 4,960,148 + Pf6N2E2_4262 0.35 +1.0 4,960,148 + Pf6N2E2_4262 0.35 +0.6 4,960,148 + Pf6N2E2_4262 0.35 -0.2 4,960,149 - Pf6N2E2_4262 0.35 +1.1 4,960,149 - Pf6N2E2_4262 0.35 -1.1 4,960,149 - Pf6N2E2_4262 0.35 +0.1 4,960,149 - Pf6N2E2_4262 0.35 +0.4 4,960,149 - Pf6N2E2_4262 0.35 +0.1 4,960,178 - Pf6N2E2_4262 0.39 -2.3 4,960,249 + Pf6N2E2_4262 0.47 -0.4 4,960,323 + Pf6N2E2_4262 0.56 +0.8 4,960,324 - Pf6N2E2_4262 0.56 +0.5 4,960,367 + Pf6N2E2_4262 0.61 +0.1 4,960,367 + Pf6N2E2_4262 0.61 -0.9 4,960,368 - Pf6N2E2_4262 0.62 +1.1 4,960,442 + Pf6N2E2_4262 0.71 -0.1 4,960,442 + Pf6N2E2_4262 0.71 +1.1 4,960,442 + Pf6N2E2_4262 0.71 -0.6 4,960,442 + Pf6N2E2_4262 0.71 +0.6 4,960,442 + Pf6N2E2_4262 0.71 -0.6 4,960,442 + Pf6N2E2_4262 0.71 +1.0 4,960,442 + Pf6N2E2_4262 0.71 +0.4 4,960,442 + Pf6N2E2_4262 0.71 -0.9 4,960,443 - Pf6N2E2_4262 0.71 +0.6 4,960,450 + Pf6N2E2_4262 0.72 -0.3 4,960,451 - Pf6N2E2_4262 0.72 -1.1 4,960,451 - Pf6N2E2_4262 0.72 -0.4 4,960,479 - Pf6N2E2_4262 0.75 +0.5 4,960,523 + Pf6N2E2_4262 0.80 +1.2 4,960,523 + Pf6N2E2_4262 0.80 +2.6 4,960,569 + Pf6N2E2_4262 0.86 +1.6 4,960,569 + Pf6N2E2_4262 0.86 +0.3 4,960,569 + Pf6N2E2_4262 0.86 -0.2 4,960,570 - Pf6N2E2_4262 0.86 +0.6 4,960,570 - Pf6N2E2_4262 0.86 -1.6 4,960,571 + Pf6N2E2_4262 0.86 +0.2 4,960,572 - Pf6N2E2_4262 0.86 +0.4 4,960,572 - Pf6N2E2_4262 0.86 +0.9 4,960,572 - Pf6N2E2_4262 0.86 -0.5 4,960,582 + Pf6N2E2_4262 0.88 +1.0 4,960,582 + Pf6N2E2_4262 0.88 +0.8 4,960,582 + Pf6N2E2_4262 0.88 -0.1 4,960,582 + Pf6N2E2_4262 0.88 +1.1 4,960,582 + Pf6N2E2_4262 0.88 -0.2 4,960,582 + Pf6N2E2_4262 0.88 -1.3 4,960,582 + Pf6N2E2_4262 0.88 +0.1 4,960,582 + Pf6N2E2_4262 0.88 +0.3 4,960,582 + Pf6N2E2_4262 0.88 -0.1 4,960,582 + Pf6N2E2_4262 0.88 +0.4 4,960,582 + Pf6N2E2_4262 0.88 +0.5 4,960,582 + Pf6N2E2_4262 0.88 -0.8 4,960,582 + Pf6N2E2_4262 0.88 -0.6 4,960,582 + Pf6N2E2_4262 0.88 -0.1 4,960,582 + Pf6N2E2_4262 0.88 -0.3 4,960,583 - Pf6N2E2_4262 0.88 +0.4 4,960,583 - Pf6N2E2_4262 0.88 -1.1 4,960,583 - Pf6N2E2_4262 0.88 +0.9 4,960,583 - Pf6N2E2_4262 0.88 -1.1 4,960,583 - Pf6N2E2_4262 0.88 +0.3 4,960,583 - Pf6N2E2_4262 0.88 +1.3 4,960,583 - Pf6N2E2_4262 0.88 +0.6 4,960,583 - Pf6N2E2_4262 0.88 +1.2 4,960,583 - Pf6N2E2_4262 0.88 -0.6 4,960,583 - Pf6N2E2_4262 0.88 +1.2 4,960,583 - Pf6N2E2_4262 0.88 +0.6 4,960,583 - Pf6N2E2_4262 0.88 +0.9 4,960,583 - Pf6N2E2_4262 0.88 +1.8
Or see this region's nucleotide sequence