Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_424 and Pf6N2E2_425 are separated by 217 nucleotides Pf6N2E2_425 and Pf6N2E2_426 are separated by 4 nucleotides Pf6N2E2_426 and Pf6N2E2_427 are separated by 161 nucleotides Pf6N2E2_427 and Pf6N2E2_428 are separated by 125 nucleotides
Pf6N2E2_424 - Decarboxylase family protein, at 492,718 to 494,091
_424
Pf6N2E2_425 - FIG00957304: hypothetical protein, at 494,309 to 494,842
_425
Pf6N2E2_426 - Arsenate reductase (EC 1.20.4.1), at 494,847 to 495,302
_426
Pf6N2E2_427 - Conidiation-specific protein 10, at 495,464 to 495,643
_427
Pf6N2E2_428 - Protease subunit of ATP-dependent Clp proteases, at 495,769 to 496,332
_428
Position (kb) 494
495 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 493.348 kb on + strand, within Pf6N2E2_424 at 493.348 kb on + strand, within Pf6N2E2_424 at 493.349 kb on - strand, within Pf6N2E2_424 at 493.470 kb on + strand, within Pf6N2E2_424 at 493.471 kb on - strand, within Pf6N2E2_424 at 493.545 kb on + strand, within Pf6N2E2_424 at 493.546 kb on - strand, within Pf6N2E2_424 at 493.589 kb on + strand, within Pf6N2E2_424 at 493.590 kb on - strand, within Pf6N2E2_424 at 493.590 kb on - strand, within Pf6N2E2_424 at 493.590 kb on - strand, within Pf6N2E2_424 at 493.595 kb on + strand, within Pf6N2E2_424 at 493.595 kb on + strand, within Pf6N2E2_424 at 493.595 kb on + strand, within Pf6N2E2_424 at 493.694 kb on + strand, within Pf6N2E2_424 at 493.695 kb on - strand, within Pf6N2E2_424 at 493.695 kb on - strand, within Pf6N2E2_424 at 493.901 kb on + strand, within Pf6N2E2_424 at 493.902 kb on - strand, within Pf6N2E2_424 at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.983 kb on - strand at 493.983 kb on - strand at 493.986 kb on + strand at 494.074 kb on + strand at 494.074 kb on + strand at 494.074 kb on + strand at 494.074 kb on + strand at 494.074 kb on + strand at 494.074 kb on + strand at 494.075 kb on - strand at 494.075 kb on - strand at 494.075 kb on - strand at 494.241 kb on + strand at 494.241 kb on + strand at 494.241 kb on + strand at 494.242 kb on - strand at 494.245 kb on + strand at 494.245 kb on + strand at 494.245 kb on + strand at 494.245 kb on + strand at 494.245 kb on + strand at 494.245 kb on + strand at 494.246 kb on - strand at 494.246 kb on - strand at 494.246 kb on - strand at 494.246 kb on - strand at 494.246 kb on - strand at 494.289 kb on + strand at 494.289 kb on + strand at 494.290 kb on - strand at 494.290 kb on - strand at 494.290 kb on - strand at 494.342 kb on + strand at 494.343 kb on - strand at 494.343 kb on - strand at 494.343 kb on - strand at 494.519 kb on + strand, within Pf6N2E2_425 at 494.520 kb on - strand, within Pf6N2E2_425 at 494.555 kb on + strand, within Pf6N2E2_425 at 494.664 kb on - strand, within Pf6N2E2_425 at 494.664 kb on - strand, within Pf6N2E2_425 at 494.828 kb on + strand at 495.046 kb on + strand, within Pf6N2E2_426 at 495.047 kb on - strand, within Pf6N2E2_426 at 495.047 kb on - strand, within Pf6N2E2_426 at 495.047 kb on - strand, within Pf6N2E2_426 at 495.147 kb on + strand, within Pf6N2E2_426 at 495.147 kb on + strand, within Pf6N2E2_426 at 495.148 kb on - strand, within Pf6N2E2_426 at 495.148 kb on - strand, within Pf6N2E2_426 at 495.148 kb on - strand, within Pf6N2E2_426 at 495.148 kb on - strand, within Pf6N2E2_426 at 495.358 kb on + strand at 495.358 kb on + strand at 495.359 kb on - strand at 495.359 kb on - strand at 495.359 kb on - strand at 495.360 kb on + strand at 495.377 kb on - strand at 495.378 kb on + strand at 495.689 kb on - strand at 495.689 kb on - strand at 495.770 kb on + strand at 495.771 kb on - strand at 495.771 kb on - strand at 495.818 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 493,348 + Pf6N2E2_424 0.46 -0.8 493,348 + Pf6N2E2_424 0.46 +0.5 493,349 - Pf6N2E2_424 0.46 +0.2 493,470 + Pf6N2E2_424 0.55 +1.2 493,471 - Pf6N2E2_424 0.55 -0.2 493,545 + Pf6N2E2_424 0.60 -2.1 493,546 - Pf6N2E2_424 0.60 +1.5 493,589 + Pf6N2E2_424 0.63 +0.9 493,590 - Pf6N2E2_424 0.63 -1.5 493,590 - Pf6N2E2_424 0.63 +1.3 493,590 - Pf6N2E2_424 0.63 -0.1 493,595 + Pf6N2E2_424 0.64 +0.7 493,595 + Pf6N2E2_424 0.64 +0.1 493,595 + Pf6N2E2_424 0.64 +1.0 493,694 + Pf6N2E2_424 0.71 +2.9 493,695 - Pf6N2E2_424 0.71 +1.0 493,695 - Pf6N2E2_424 0.71 -1.0 493,901 + Pf6N2E2_424 0.86 +1.6 493,902 - Pf6N2E2_424 0.86 +0.3 493,982 + -1.2 493,982 + +1.3 493,982 + -0.2 493,982 + +0.5 493,982 + +2.3 493,982 + +0.5 493,982 + +0.3 493,982 + -0.5 493,982 + -1.2 493,983 - +0.3 493,983 - +1.3 493,986 + -0.8 494,074 + -0.5 494,074 + +2.3 494,074 + +1.1 494,074 + +0.3 494,074 + -0.5 494,074 + +0.5 494,075 - +0.5 494,075 - -0.0 494,075 - +1.2 494,241 + -0.1 494,241 + -0.8 494,241 + -0.6 494,242 - -2.7 494,245 + +1.4 494,245 + +0.5 494,245 + -0.4 494,245 + +1.8 494,245 + +1.4 494,245 + +1.1 494,246 - -2.2 494,246 - -2.0 494,246 - -0.7 494,246 - -1.8 494,246 - -2.4 494,289 + +1.0 494,289 + +1.0 494,290 - -2.2 494,290 - -0.4 494,290 - -0.1 494,342 + -0.4 494,343 - -0.4 494,343 - -1.2 494,343 - -1.2 494,519 + Pf6N2E2_425 0.39 +0.8 494,520 - Pf6N2E2_425 0.40 -1.1 494,555 + Pf6N2E2_425 0.46 -2.3 494,664 - Pf6N2E2_425 0.66 -2.5 494,664 - Pf6N2E2_425 0.66 -0.9 494,828 + -0.6 495,046 + Pf6N2E2_426 0.44 -0.2 495,047 - Pf6N2E2_426 0.44 +0.3 495,047 - Pf6N2E2_426 0.44 +0.2 495,047 - Pf6N2E2_426 0.44 +0.4 495,147 + Pf6N2E2_426 0.66 +0.8 495,147 + Pf6N2E2_426 0.66 +1.6 495,148 - Pf6N2E2_426 0.66 -0.2 495,148 - Pf6N2E2_426 0.66 +1.5 495,148 - Pf6N2E2_426 0.66 -0.6 495,148 - Pf6N2E2_426 0.66 -0.3 495,358 + +1.1 495,358 + +1.6 495,359 - +0.4 495,359 - -0.0 495,359 - +1.9 495,360 + -0.6 495,377 - +0.8 495,378 + -0.6 495,689 - +0.1 495,689 - +0.4 495,770 + +1.4 495,771 - +1.3 495,771 - +1.1 495,818 - -0.5
Or see this region's nucleotide sequence