Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4217 and Pf6N2E2_4218 are separated by 316 nucleotides Pf6N2E2_4218 and Pf6N2E2_4219 overlap by 4 nucleotides Pf6N2E2_4219 and Pf6N2E2_4220 overlap by 4 nucleotides Pf6N2E2_4220 and Pf6N2E2_4221 are separated by 15 nucleotides
Pf6N2E2_4217 - Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16), at 4,907,997 to 4,909,829
_4217
Pf6N2E2_4218 - hypothetical protein, at 4,910,146 to 4,910,574
_4218
Pf6N2E2_4219 - hypothetical protein, at 4,910,571 to 4,910,864
_4219
Pf6N2E2_4220 - RNA polymerase sigma factor RpoE, at 4,910,861 to 4,911,436
_4220
Pf6N2E2_4221 - hypothetical protein, at 4,911,452 to 4,911,775
_4221
Position (kb)
4910
4911 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4909.870 kb on + strand at 4909.977 kb on + strand at 4909.978 kb on - strand at 4909.979 kb on + strand at 4909.979 kb on + strand at 4909.979 kb on + strand at 4909.979 kb on + strand at 4909.980 kb on - strand at 4909.984 kb on + strand at 4909.984 kb on + strand at 4909.984 kb on + strand at 4909.985 kb on - strand at 4909.985 kb on - strand at 4909.985 kb on - strand at 4910.043 kb on - strand at 4910.163 kb on + strand at 4910.164 kb on - strand at 4910.164 kb on - strand at 4910.164 kb on - strand at 4910.189 kb on - strand, within Pf6N2E2_4218 at 4910.201 kb on - strand, within Pf6N2E2_4218 at 4910.214 kb on + strand, within Pf6N2E2_4218 at 4910.214 kb on + strand, within Pf6N2E2_4218 at 4910.214 kb on + strand, within Pf6N2E2_4218 at 4910.214 kb on + strand, within Pf6N2E2_4218 at 4910.275 kb on + strand, within Pf6N2E2_4218 at 4910.275 kb on + strand, within Pf6N2E2_4218 at 4910.276 kb on - strand, within Pf6N2E2_4218 at 4910.276 kb on - strand, within Pf6N2E2_4218 at 4910.276 kb on - strand, within Pf6N2E2_4218 at 4910.276 kb on - strand, within Pf6N2E2_4218 at 4910.313 kb on + strand, within Pf6N2E2_4218 at 4910.314 kb on - strand, within Pf6N2E2_4218 at 4910.317 kb on + strand, within Pf6N2E2_4218 at 4910.317 kb on + strand, within Pf6N2E2_4218 at 4910.317 kb on + strand, within Pf6N2E2_4218 at 4910.317 kb on + strand, within Pf6N2E2_4218 at 4910.317 kb on + strand, within Pf6N2E2_4218 at 4910.318 kb on - strand, within Pf6N2E2_4218 at 4910.318 kb on - strand, within Pf6N2E2_4218 at 4910.318 kb on - strand, within Pf6N2E2_4218 at 4910.318 kb on - strand, within Pf6N2E2_4218 at 4910.318 kb on - strand, within Pf6N2E2_4218 at 4910.318 kb on - strand, within Pf6N2E2_4218 at 4910.320 kb on + strand, within Pf6N2E2_4218 at 4910.320 kb on + strand, within Pf6N2E2_4218 at 4910.320 kb on + strand, within Pf6N2E2_4218 at 4910.321 kb on - strand, within Pf6N2E2_4218 at 4910.321 kb on - strand, within Pf6N2E2_4218 at 4910.321 kb on - strand, within Pf6N2E2_4218 at 4910.321 kb on - strand, within Pf6N2E2_4218 at 4910.425 kb on + strand, within Pf6N2E2_4218 at 4910.426 kb on - strand, within Pf6N2E2_4218 at 4910.498 kb on - strand, within Pf6N2E2_4218 at 4910.498 kb on - strand, within Pf6N2E2_4218 at 4910.574 kb on + strand at 4910.574 kb on + strand at 4910.574 kb on + strand at 4910.575 kb on - strand at 4910.575 kb on - strand at 4910.640 kb on - strand, within Pf6N2E2_4219 at 4910.915 kb on + strand at 4910.915 kb on + strand at 4910.915 kb on + strand at 4910.916 kb on - strand at 4910.916 kb on - strand at 4910.916 kb on - strand at 4910.916 kb on - strand at 4911.009 kb on + strand, within Pf6N2E2_4220 at 4911.169 kb on + strand, within Pf6N2E2_4220 at 4911.169 kb on + strand, within Pf6N2E2_4220 at 4911.169 kb on + strand, within Pf6N2E2_4220 at 4911.169 kb on + strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.208 kb on + strand, within Pf6N2E2_4220 at 4911.208 kb on + strand, within Pf6N2E2_4220 at 4911.208 kb on + strand, within Pf6N2E2_4220 at 4911.209 kb on - strand, within Pf6N2E2_4220 at 4911.223 kb on + strand, within Pf6N2E2_4220 at 4911.223 kb on + strand, within Pf6N2E2_4220 at 4911.223 kb on + strand, within Pf6N2E2_4220 at 4911.223 kb on + strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.343 kb on - strand, within Pf6N2E2_4220 at 4911.343 kb on - strand, within Pf6N2E2_4220 at 4911.343 kb on - strand, within Pf6N2E2_4220 at 4911.343 kb on - strand, within Pf6N2E2_4220 at 4911.343 kb on - strand, within Pf6N2E2_4220 at 4911.343 kb on - strand, within Pf6N2E2_4220 at 4911.376 kb on + strand, within Pf6N2E2_4220 at 4911.376 kb on + strand, within Pf6N2E2_4220 at 4911.376 kb on + strand, within Pf6N2E2_4220 at 4911.377 kb on - strand, within Pf6N2E2_4220 at 4911.377 kb on - strand, within Pf6N2E2_4220 at 4911.385 kb on - strand at 4911.413 kb on + strand at 4911.414 kb on - strand at 4911.414 kb on - strand at 4911.414 kb on - strand at 4911.414 kb on - strand at 4911.453 kb on + strand at 4911.453 kb on + strand at 4911.454 kb on - strand at 4911.454 kb on - strand at 4911.529 kb on + strand, within Pf6N2E2_4221 at 4911.530 kb on - strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.549 kb on + strand, within Pf6N2E2_4221 at 4911.549 kb on + strand, within Pf6N2E2_4221 at 4911.550 kb on - strand, within Pf6N2E2_4221 at 4911.550 kb on - strand, within Pf6N2E2_4221 at 4911.550 kb on - strand, within Pf6N2E2_4221 at 4911.550 kb on - strand, within Pf6N2E2_4221
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,909,870 + +2.1 4,909,977 + -1.5 4,909,978 - +0.3 4,909,979 + +0.9 4,909,979 + -0.7 4,909,979 + -0.1 4,909,979 + +0.1 4,909,980 - +0.2 4,909,984 + +0.5 4,909,984 + -0.4 4,909,984 + -0.6 4,909,985 - +0.6 4,909,985 - +0.2 4,909,985 - +0.7 4,910,043 - +0.4 4,910,163 + -0.1 4,910,164 - +0.2 4,910,164 - +0.1 4,910,164 - -1.3 4,910,189 - Pf6N2E2_4218 0.10 +0.4 4,910,201 - Pf6N2E2_4218 0.13 -1.9 4,910,214 + Pf6N2E2_4218 0.16 -1.9 4,910,214 + Pf6N2E2_4218 0.16 -0.4 4,910,214 + Pf6N2E2_4218 0.16 -1.6 4,910,214 + Pf6N2E2_4218 0.16 +2.1 4,910,275 + Pf6N2E2_4218 0.30 +0.1 4,910,275 + Pf6N2E2_4218 0.30 -0.2 4,910,276 - Pf6N2E2_4218 0.30 +0.6 4,910,276 - Pf6N2E2_4218 0.30 -0.9 4,910,276 - Pf6N2E2_4218 0.30 +0.4 4,910,276 - Pf6N2E2_4218 0.30 -0.4 4,910,313 + Pf6N2E2_4218 0.39 +0.6 4,910,314 - Pf6N2E2_4218 0.39 -0.8 4,910,317 + Pf6N2E2_4218 0.40 -0.8 4,910,317 + Pf6N2E2_4218 0.40 +1.6 4,910,317 + Pf6N2E2_4218 0.40 -0.7 4,910,317 + Pf6N2E2_4218 0.40 +0.0 4,910,317 + Pf6N2E2_4218 0.40 -0.6 4,910,318 - Pf6N2E2_4218 0.40 -1.1 4,910,318 - Pf6N2E2_4218 0.40 -1.1 4,910,318 - Pf6N2E2_4218 0.40 -1.4 4,910,318 - Pf6N2E2_4218 0.40 -2.0 4,910,318 - Pf6N2E2_4218 0.40 -0.6 4,910,318 - Pf6N2E2_4218 0.40 -0.2 4,910,320 + Pf6N2E2_4218 0.41 -2.0 4,910,320 + Pf6N2E2_4218 0.41 -0.6 4,910,320 + Pf6N2E2_4218 0.41 -0.9 4,910,321 - Pf6N2E2_4218 0.41 -1.3 4,910,321 - Pf6N2E2_4218 0.41 +0.3 4,910,321 - Pf6N2E2_4218 0.41 +0.4 4,910,321 - Pf6N2E2_4218 0.41 +0.4 4,910,425 + Pf6N2E2_4218 0.65 -0.9 4,910,426 - Pf6N2E2_4218 0.65 -0.9 4,910,498 - Pf6N2E2_4218 0.82 +0.7 4,910,498 - Pf6N2E2_4218 0.82 -3.0 4,910,574 + +0.4 4,910,574 + -0.1 4,910,574 + -2.4 4,910,575 - -1.0 4,910,575 - -0.8 4,910,640 - Pf6N2E2_4219 0.23 -1.7 4,910,915 + +0.6 4,910,915 + +2.2 4,910,915 + +0.5 4,910,916 - +0.7 4,910,916 - +0.5 4,910,916 - -0.6 4,910,916 - +1.3 4,911,009 + Pf6N2E2_4220 0.26 -0.9 4,911,169 + Pf6N2E2_4220 0.53 +1.3 4,911,169 + Pf6N2E2_4220 0.53 -0.7 4,911,169 + Pf6N2E2_4220 0.53 -0.1 4,911,169 + Pf6N2E2_4220 0.53 +0.9 4,911,170 - Pf6N2E2_4220 0.54 -0.3 4,911,170 - Pf6N2E2_4220 0.54 -0.3 4,911,170 - Pf6N2E2_4220 0.54 +0.3 4,911,170 - Pf6N2E2_4220 0.54 -0.4 4,911,170 - Pf6N2E2_4220 0.54 -0.5 4,911,170 - Pf6N2E2_4220 0.54 +0.0 4,911,170 - Pf6N2E2_4220 0.54 -1.1 4,911,208 + Pf6N2E2_4220 0.60 +0.6 4,911,208 + Pf6N2E2_4220 0.60 +0.6 4,911,208 + Pf6N2E2_4220 0.60 -0.7 4,911,209 - Pf6N2E2_4220 0.60 +0.6 4,911,223 + Pf6N2E2_4220 0.63 -0.2 4,911,223 + Pf6N2E2_4220 0.63 -0.2 4,911,223 + Pf6N2E2_4220 0.63 +1.5 4,911,223 + Pf6N2E2_4220 0.63 +0.1 4,911,224 - Pf6N2E2_4220 0.63 +1.0 4,911,224 - Pf6N2E2_4220 0.63 -1.3 4,911,224 - Pf6N2E2_4220 0.63 -0.1 4,911,224 - Pf6N2E2_4220 0.63 +0.8 4,911,224 - Pf6N2E2_4220 0.63 +0.5 4,911,224 - Pf6N2E2_4220 0.63 +0.2 4,911,224 - Pf6N2E2_4220 0.63 +0.3 4,911,224 - Pf6N2E2_4220 0.63 +0.4 4,911,224 - Pf6N2E2_4220 0.63 -3.8 4,911,224 - Pf6N2E2_4220 0.63 +0.3 4,911,224 - Pf6N2E2_4220 0.63 +1.2 4,911,224 - Pf6N2E2_4220 0.63 +0.9 4,911,224 - Pf6N2E2_4220 0.63 +0.3 4,911,224 - Pf6N2E2_4220 0.63 +1.6 4,911,342 + Pf6N2E2_4220 0.84 -0.5 4,911,342 + Pf6N2E2_4220 0.84 +0.6 4,911,342 + Pf6N2E2_4220 0.84 -0.5 4,911,342 + Pf6N2E2_4220 0.84 +0.5 4,911,342 + Pf6N2E2_4220 0.84 +0.6 4,911,342 + Pf6N2E2_4220 0.84 +0.5 4,911,342 + Pf6N2E2_4220 0.84 -0.1 4,911,342 + Pf6N2E2_4220 0.84 +1.0 4,911,342 + Pf6N2E2_4220 0.84 +1.0 4,911,342 + Pf6N2E2_4220 0.84 +0.5 4,911,342 + Pf6N2E2_4220 0.84 +0.1 4,911,343 - Pf6N2E2_4220 0.84 -1.1 4,911,343 - Pf6N2E2_4220 0.84 +0.7 4,911,343 - Pf6N2E2_4220 0.84 -0.2 4,911,343 - Pf6N2E2_4220 0.84 -0.5 4,911,343 - Pf6N2E2_4220 0.84 +0.0 4,911,343 - Pf6N2E2_4220 0.84 -0.2 4,911,376 + Pf6N2E2_4220 0.89 +0.4 4,911,376 + Pf6N2E2_4220 0.89 -0.6 4,911,376 + Pf6N2E2_4220 0.89 +0.9 4,911,377 - Pf6N2E2_4220 0.90 +0.6 4,911,377 - Pf6N2E2_4220 0.90 -0.7 4,911,385 - -0.1 4,911,413 + -0.4 4,911,414 - -1.1 4,911,414 - +1.1 4,911,414 - +0.2 4,911,414 - -1.2 4,911,453 + +0.3 4,911,453 + -0.5 4,911,454 - +1.5 4,911,454 - -0.0 4,911,529 + Pf6N2E2_4221 0.24 +0.1 4,911,530 - Pf6N2E2_4221 0.24 -0.3 4,911,543 + Pf6N2E2_4221 0.28 -1.0 4,911,543 + Pf6N2E2_4221 0.28 -0.1 4,911,543 + Pf6N2E2_4221 0.28 -0.9 4,911,543 + Pf6N2E2_4221 0.28 +2.0 4,911,543 + Pf6N2E2_4221 0.28 -0.0 4,911,543 + Pf6N2E2_4221 0.28 -0.2 4,911,543 + Pf6N2E2_4221 0.28 +1.5 4,911,543 + Pf6N2E2_4221 0.28 -0.4 4,911,543 + Pf6N2E2_4221 0.28 +0.2 4,911,544 - Pf6N2E2_4221 0.28 +1.3 4,911,544 - Pf6N2E2_4221 0.28 +0.3 4,911,544 - Pf6N2E2_4221 0.28 -1.1 4,911,544 - Pf6N2E2_4221 0.28 +0.4 4,911,544 - Pf6N2E2_4221 0.28 +0.5 4,911,544 - Pf6N2E2_4221 0.28 +0.5 4,911,544 - Pf6N2E2_4221 0.28 +0.5 4,911,544 - Pf6N2E2_4221 0.28 +0.2 4,911,549 + Pf6N2E2_4221 0.30 -0.2 4,911,549 + Pf6N2E2_4221 0.30 +0.5 4,911,550 - Pf6N2E2_4221 0.30 +0.4 4,911,550 - Pf6N2E2_4221 0.30 +0.5 4,911,550 - Pf6N2E2_4221 0.30 +0.1 4,911,550 - Pf6N2E2_4221 0.30 -0.5
Or see this region's nucleotide sequence