Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4164 and Pf6N2E2_4165 are separated by 165 nucleotides Pf6N2E2_4165 and Pf6N2E2_4166 are separated by 114 nucleotides Pf6N2E2_4166 and Pf6N2E2_4167 overlap by 1 nucleotides
Pf6N2E2_4164 - Homoserine kinase (EC 2.7.1.39), at 4,846,508 to 4,847,458
_4164
Pf6N2E2_4165 - Zinc ABC transporter, periplasmic-binding protein ZnuA, at 4,847,624 to 4,848,553
_4165
Pf6N2E2_4166 - Zinc uptake regulation protein ZUR, at 4,848,668 to 4,849,102
_4166
Pf6N2E2_4167 - Zinc ABC transporter, ATP-binding protein ZnuC, at 4,849,102 to 4,849,884
_4167
Position (kb)
4847
4848
4849 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.457 kb on - strand at 4847.457 kb on - strand at 4847.457 kb on - strand at 4847.457 kb on - strand at 4847.457 kb on - strand at 4847.457 kb on - strand at 4847.457 kb on - strand at 4847.460 kb on + strand at 4847.460 kb on + strand at 4847.460 kb on + strand at 4847.460 kb on + strand at 4847.461 kb on - strand at 4847.461 kb on - strand at 4847.461 kb on - strand at 4847.461 kb on - strand at 4847.461 kb on - strand at 4847.461 kb on - strand at 4847.627 kb on + strand at 4847.627 kb on + strand at 4847.627 kb on + strand at 4847.627 kb on + strand at 4847.627 kb on + strand at 4847.628 kb on - strand at 4847.628 kb on - strand at 4847.628 kb on - strand at 4847.628 kb on - strand at 4847.682 kb on + strand at 4847.706 kb on + strand at 4847.706 kb on + strand at 4847.706 kb on + strand at 4847.706 kb on + strand at 4847.707 kb on - strand at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.908 kb on - strand, within Pf6N2E2_4165 at 4847.908 kb on - strand, within Pf6N2E2_4165 at 4847.908 kb on - strand, within Pf6N2E2_4165 at 4847.923 kb on - strand, within Pf6N2E2_4165 at 4847.949 kb on + strand, within Pf6N2E2_4165 at 4847.950 kb on - strand, within Pf6N2E2_4165 at 4847.950 kb on - strand, within Pf6N2E2_4165 at 4848.322 kb on - strand, within Pf6N2E2_4165 at 4848.372 kb on + strand, within Pf6N2E2_4165 at 4848.373 kb on - strand, within Pf6N2E2_4165 at 4848.507 kb on - strand at 4848.566 kb on + strand at 4848.566 kb on + strand at 4848.566 kb on + strand at 4848.567 kb on - strand at 4848.587 kb on + strand at 4848.588 kb on - strand at 4848.625 kb on + strand at 4848.625 kb on + strand at 4848.626 kb on - strand at 4848.626 kb on - strand at 4848.626 kb on - strand at 4848.628 kb on + strand at 4848.628 kb on + strand at 4848.628 kb on + strand at 4848.629 kb on - strand at 4848.629 kb on - strand at 4848.629 kb on - strand at 4848.629 kb on - strand at 4848.629 kb on - strand at 4849.315 kb on + strand, within Pf6N2E2_4167 at 4849.315 kb on + strand, within Pf6N2E2_4167 at 4849.315 kb on + strand, within Pf6N2E2_4167 at 4849.316 kb on - strand, within Pf6N2E2_4167 at 4849.317 kb on + strand, within Pf6N2E2_4167 at 4849.317 kb on + strand, within Pf6N2E2_4167 at 4849.318 kb on - strand, within Pf6N2E2_4167 at 4849.318 kb on - strand, within Pf6N2E2_4167 at 4849.490 kb on + strand, within Pf6N2E2_4167 at 4849.490 kb on + strand, within Pf6N2E2_4167
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,847,456 + -0.7 4,847,456 + -3.5 4,847,456 + +0.0 4,847,456 + -0.1 4,847,456 + +1.9 4,847,456 + +0.7 4,847,456 + -0.6 4,847,456 + -0.6 4,847,456 + +1.2 4,847,456 + -0.4 4,847,457 - -0.2 4,847,457 - -0.9 4,847,457 - +0.9 4,847,457 - +0.1 4,847,457 - -0.4 4,847,457 - +0.7 4,847,457 - -0.3 4,847,460 + -0.9 4,847,460 + -0.2 4,847,460 + +0.8 4,847,460 + -0.8 4,847,461 - +0.5 4,847,461 - -0.5 4,847,461 - -0.4 4,847,461 - -0.3 4,847,461 - +0.7 4,847,461 - +1.2 4,847,627 + -0.2 4,847,627 + -0.5 4,847,627 + +1.4 4,847,627 + -0.8 4,847,627 + -2.5 4,847,628 - -0.3 4,847,628 - -0.0 4,847,628 - -0.4 4,847,628 - -1.2 4,847,682 + +1.8 4,847,706 + +2.1 4,847,706 + +2.0 4,847,706 + +1.6 4,847,706 + +2.6 4,847,707 - +1.3 4,847,907 + Pf6N2E2_4165 0.30 +2.0 4,847,907 + Pf6N2E2_4165 0.30 +2.0 4,847,907 + Pf6N2E2_4165 0.30 +1.6 4,847,907 + Pf6N2E2_4165 0.30 +1.2 4,847,907 + Pf6N2E2_4165 0.30 +0.5 4,847,907 + Pf6N2E2_4165 0.30 +0.6 4,847,907 + Pf6N2E2_4165 0.30 +0.9 4,847,908 - Pf6N2E2_4165 0.31 +0.7 4,847,908 - Pf6N2E2_4165 0.31 +1.4 4,847,908 - Pf6N2E2_4165 0.31 +1.9 4,847,923 - Pf6N2E2_4165 0.32 +2.0 4,847,949 + Pf6N2E2_4165 0.35 +1.1 4,847,950 - Pf6N2E2_4165 0.35 +1.6 4,847,950 - Pf6N2E2_4165 0.35 +1.6 4,848,322 - Pf6N2E2_4165 0.75 -1.4 4,848,372 + Pf6N2E2_4165 0.80 -0.5 4,848,373 - Pf6N2E2_4165 0.81 +3.2 4,848,507 - +2.3 4,848,566 + -0.6 4,848,566 + -1.9 4,848,566 + +1.3 4,848,567 - +1.6 4,848,587 + +0.6 4,848,588 - -0.4 4,848,625 + +2.1 4,848,625 + +2.1 4,848,626 - -0.4 4,848,626 - +0.2 4,848,626 - +0.9 4,848,628 + +1.0 4,848,628 + -0.2 4,848,628 + +1.1 4,848,629 - +1.5 4,848,629 - +0.1 4,848,629 - +0.9 4,848,629 - +1.2 4,848,629 - +0.5 4,849,315 + Pf6N2E2_4167 0.27 +2.1 4,849,315 + Pf6N2E2_4167 0.27 +1.9 4,849,315 + Pf6N2E2_4167 0.27 +2.1 4,849,316 - Pf6N2E2_4167 0.27 +2.4 4,849,317 + Pf6N2E2_4167 0.27 +2.6 4,849,317 + Pf6N2E2_4167 0.27 +1.6 4,849,318 - Pf6N2E2_4167 0.28 +2.0 4,849,318 - Pf6N2E2_4167 0.28 +0.8 4,849,490 + Pf6N2E2_4167 0.50 -0.4 4,849,490 + Pf6N2E2_4167 0.50 +2.4
Or see this region's nucleotide sequence