Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4156

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4155 and Pf6N2E2_4156 are separated by 104 nucleotidesPf6N2E2_4156 and Pf6N2E2_4157 overlap by 4 nucleotidesPf6N2E2_4157 and Pf6N2E2_4158 are separated by 15 nucleotides Pf6N2E2_4155 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 4,836,481 to 4,837,260 _4155 Pf6N2E2_4156 - GGDEF domain protein, at 4,837,365 to 4,839,431 _4156 Pf6N2E2_4157 - Endonuclease/exonuclease/phosphatase family protein, at 4,839,428 to 4,840,306 _4157 Pf6N2E2_4158 - Periplasmic thiol:disulfide interchange protein DsbA, at 4,840,322 to 4,840,963 _4158 Position (kb) 4837 4838 4839 4840Strain fitness (log2 ratio) -3 -2 -1 0 1at 4836.382 kb on + strandat 4836.382 kb on + strandat 4836.382 kb on + strandat 4836.425 kb on - strandat 4836.436 kb on - strandat 4836.482 kb on + strandat 4836.557 kb on + strandat 4836.730 kb on + strand, within Pf6N2E2_4155at 4836.730 kb on + strand, within Pf6N2E2_4155at 4836.731 kb on - strand, within Pf6N2E2_4155at 4836.731 kb on - strand, within Pf6N2E2_4155at 4836.862 kb on + strand, within Pf6N2E2_4155at 4836.862 kb on + strand, within Pf6N2E2_4155at 4836.862 kb on + strand, within Pf6N2E2_4155at 4836.862 kb on + strand, within Pf6N2E2_4155at 4836.862 kb on + strand, within Pf6N2E2_4155at 4836.863 kb on - strand, within Pf6N2E2_4155at 4836.863 kb on - strand, within Pf6N2E2_4155at 4836.894 kb on - strand, within Pf6N2E2_4155at 4836.894 kb on - strand, within Pf6N2E2_4155at 4836.899 kb on + strand, within Pf6N2E2_4155at 4837.040 kb on + strand, within Pf6N2E2_4155at 4837.040 kb on + strand, within Pf6N2E2_4155at 4837.041 kb on - strand, within Pf6N2E2_4155at 4837.041 kb on - strand, within Pf6N2E2_4155at 4837.074 kb on - strand, within Pf6N2E2_4155at 4837.074 kb on - strand, within Pf6N2E2_4155at 4837.136 kb on + strand, within Pf6N2E2_4155at 4837.136 kb on + strand, within Pf6N2E2_4155at 4837.136 kb on + strand, within Pf6N2E2_4155at 4837.136 kb on + strand, within Pf6N2E2_4155at 4837.136 kb on + strand, within Pf6N2E2_4155at 4837.136 kb on + strand, within Pf6N2E2_4155at 4837.136 kb on + strand, within Pf6N2E2_4155at 4837.136 kb on + strand, within Pf6N2E2_4155at 4837.137 kb on - strand, within Pf6N2E2_4155at 4837.137 kb on - strand, within Pf6N2E2_4155at 4837.137 kb on - strand, within Pf6N2E2_4155at 4837.137 kb on - strand, within Pf6N2E2_4155at 4837.137 kb on - strand, within Pf6N2E2_4155at 4837.137 kb on - strand, within Pf6N2E2_4155at 4837.137 kb on - strand, within Pf6N2E2_4155at 4837.158 kb on + strand, within Pf6N2E2_4155at 4837.159 kb on - strand, within Pf6N2E2_4155at 4837.231 kb on + strandat 4837.231 kb on + strandat 4837.232 kb on - strandat 4837.232 kb on - strandat 4837.232 kb on - strandat 4837.232 kb on - strandat 4837.232 kb on - strandat 4837.273 kb on + strandat 4837.274 kb on - strandat 4837.299 kb on + strandat 4837.340 kb on + strandat 4837.340 kb on + strandat 4837.341 kb on - strandat 4837.341 kb on - strandat 4837.358 kb on + strandat 4837.359 kb on - strandat 4837.359 kb on - strandat 4837.580 kb on + strand, within Pf6N2E2_4156at 4837.581 kb on - strand, within Pf6N2E2_4156at 4837.581 kb on - strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.702 kb on + strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4837.703 kb on - strand, within Pf6N2E2_4156at 4838.200 kb on + strand, within Pf6N2E2_4156at 4838.200 kb on + strand, within Pf6N2E2_4156at 4838.201 kb on - strand, within Pf6N2E2_4156at 4838.407 kb on + strand, within Pf6N2E2_4156at 4838.408 kb on - strand, within Pf6N2E2_4156at 4838.408 kb on - strand, within Pf6N2E2_4156at 4838.408 kb on - strand, within Pf6N2E2_4156at 4838.408 kb on - strand, within Pf6N2E2_4156at 4838.408 kb on - strand, within Pf6N2E2_4156at 4838.408 kb on - strand, within Pf6N2E2_4156at 4838.408 kb on - strand, within Pf6N2E2_4156at 4838.437 kb on + strand, within Pf6N2E2_4156at 4838.437 kb on + strand, within Pf6N2E2_4156at 4838.577 kb on + strand, within Pf6N2E2_4156at 4838.578 kb on - strand, within Pf6N2E2_4156at 4838.578 kb on - strand, within Pf6N2E2_4156at 4839.186 kb on + strand, within Pf6N2E2_4156at 4839.387 kb on - strandat 4839.397 kb on + strandat 4839.397 kb on + strandat 4839.397 kb on + strandat 4839.397 kb on + strandat 4839.398 kb on - strandat 4839.398 kb on - strandat 4839.398 kb on - strandat 4839.398 kb on - strandat 4839.398 kb on - strandat 4839.398 kb on - strandat 4839.753 kb on + strand, within Pf6N2E2_4157at 4839.754 kb on - strand, within Pf6N2E2_4157at 4839.967 kb on - strand, within Pf6N2E2_4157at 4839.967 kb on - strand, within Pf6N2E2_4157at 4839.967 kb on - strand, within Pf6N2E2_4157at 4840.017 kb on + strand, within Pf6N2E2_4157at 4840.017 kb on + strand, within Pf6N2E2_4157at 4840.152 kb on + strand, within Pf6N2E2_4157at 4840.152 kb on + strand, within Pf6N2E2_4157at 4840.152 kb on + strand, within Pf6N2E2_4157at 4840.153 kb on - strand, within Pf6N2E2_4157at 4840.153 kb on - strand, within Pf6N2E2_4157at 4840.323 kb on + strandat 4840.323 kb on + strandat 4840.323 kb on + strandat 4840.323 kb on + strandat 4840.323 kb on + strandat 4840.324 kb on - strandat 4840.324 kb on - strandat 4840.324 kb on - strandat 4840.324 kb on - strandat 4840.324 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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4,836,382 + -0.5
4,836,382 + -0.8
4,836,382 + +0.1
4,836,425 - -0.6
4,836,436 - +0.7
4,836,482 + -1.0
4,836,557 + -2.9
4,836,730 + Pf6N2E2_4155 0.32 -3.2
4,836,730 + Pf6N2E2_4155 0.32 -0.5
4,836,731 - Pf6N2E2_4155 0.32 -0.5
4,836,731 - Pf6N2E2_4155 0.32 -1.1
4,836,862 + Pf6N2E2_4155 0.49 -0.4
4,836,862 + Pf6N2E2_4155 0.49 -3.1
4,836,862 + Pf6N2E2_4155 0.49 -2.4
4,836,862 + Pf6N2E2_4155 0.49 -1.4
4,836,862 + Pf6N2E2_4155 0.49 -2.2
4,836,863 - Pf6N2E2_4155 0.49 -1.9
4,836,863 - Pf6N2E2_4155 0.49 -0.9
4,836,894 - Pf6N2E2_4155 0.53 -2.7
4,836,894 - Pf6N2E2_4155 0.53 -0.8
4,836,899 + Pf6N2E2_4155 0.54 -0.1
4,837,040 + Pf6N2E2_4155 0.72 -1.6
4,837,040 + Pf6N2E2_4155 0.72 -2.1
4,837,041 - Pf6N2E2_4155 0.72 -1.6
4,837,041 - Pf6N2E2_4155 0.72 -0.9
4,837,074 - Pf6N2E2_4155 0.76 -1.5
4,837,074 - Pf6N2E2_4155 0.76 -2.8
4,837,136 + Pf6N2E2_4155 0.84 -1.3
4,837,136 + Pf6N2E2_4155 0.84 -2.9
4,837,136 + Pf6N2E2_4155 0.84 -1.1
4,837,136 + Pf6N2E2_4155 0.84 -2.2
4,837,136 + Pf6N2E2_4155 0.84 -1.4
4,837,136 + Pf6N2E2_4155 0.84 -0.0
4,837,136 + Pf6N2E2_4155 0.84 -2.4
4,837,136 + Pf6N2E2_4155 0.84 +0.2
4,837,137 - Pf6N2E2_4155 0.84 -0.8
4,837,137 - Pf6N2E2_4155 0.84 -0.5
4,837,137 - Pf6N2E2_4155 0.84 -0.1
4,837,137 - Pf6N2E2_4155 0.84 -1.6
4,837,137 - Pf6N2E2_4155 0.84 -1.6
4,837,137 - Pf6N2E2_4155 0.84 -0.4
4,837,137 - Pf6N2E2_4155 0.84 -1.7
4,837,158 + Pf6N2E2_4155 0.87 -1.4
4,837,159 - Pf6N2E2_4155 0.87 -1.4
4,837,231 + -1.8
4,837,231 + -1.7
4,837,232 - -2.2
4,837,232 - -2.0
4,837,232 - -2.4
4,837,232 - -1.2
4,837,232 - -0.7
4,837,273 + -2.1
4,837,274 - +1.3
4,837,299 + -0.6
4,837,340 + +0.3
4,837,340 + -0.6
4,837,341 - +0.0
4,837,341 - +0.5
4,837,358 + -2.0
4,837,359 - +0.2
4,837,359 - -0.1
4,837,580 + Pf6N2E2_4156 0.10 -0.8
4,837,581 - Pf6N2E2_4156 0.10 -0.4
4,837,581 - Pf6N2E2_4156 0.10 +1.2
4,837,702 + Pf6N2E2_4156 0.16 +0.1
4,837,702 + Pf6N2E2_4156 0.16 -0.9
4,837,702 + Pf6N2E2_4156 0.16 +0.2
4,837,702 + Pf6N2E2_4156 0.16 -1.2
4,837,702 + Pf6N2E2_4156 0.16 +0.3
4,837,702 + Pf6N2E2_4156 0.16 -0.1
4,837,702 + Pf6N2E2_4156 0.16 -1.3
4,837,702 + Pf6N2E2_4156 0.16 +0.2
4,837,702 + Pf6N2E2_4156 0.16 -0.6
4,837,702 + Pf6N2E2_4156 0.16 -0.6
4,837,702 + Pf6N2E2_4156 0.16 -0.5
4,837,702 + Pf6N2E2_4156 0.16 -1.3
4,837,702 + Pf6N2E2_4156 0.16 +0.9
4,837,702 + Pf6N2E2_4156 0.16 -0.2
4,837,702 + Pf6N2E2_4156 0.16 -1.1
4,837,702 + Pf6N2E2_4156 0.16 -0.1
4,837,702 + Pf6N2E2_4156 0.16 +0.5
4,837,703 - Pf6N2E2_4156 0.16 +0.3
4,837,703 - Pf6N2E2_4156 0.16 +0.4
4,837,703 - Pf6N2E2_4156 0.16 +0.7
4,837,703 - Pf6N2E2_4156 0.16 -0.2
4,837,703 - Pf6N2E2_4156 0.16 +0.4
4,837,703 - Pf6N2E2_4156 0.16 +0.6
4,837,703 - Pf6N2E2_4156 0.16 -0.1
4,837,703 - Pf6N2E2_4156 0.16 +0.6
4,837,703 - Pf6N2E2_4156 0.16 +0.3
4,837,703 - Pf6N2E2_4156 0.16 +0.2
4,837,703 - Pf6N2E2_4156 0.16 -0.4
4,837,703 - Pf6N2E2_4156 0.16 -0.6
4,837,703 - Pf6N2E2_4156 0.16 +0.2
4,837,703 - Pf6N2E2_4156 0.16 -1.0
4,837,703 - Pf6N2E2_4156 0.16 +0.8
4,837,703 - Pf6N2E2_4156 0.16 +0.3
4,837,703 - Pf6N2E2_4156 0.16 +0.8
4,837,703 - Pf6N2E2_4156 0.16 -1.5
4,838,200 + Pf6N2E2_4156 0.40 +0.1
4,838,200 + Pf6N2E2_4156 0.40 -0.6
4,838,201 - Pf6N2E2_4156 0.40 -0.8
4,838,407 + Pf6N2E2_4156 0.50 -0.6
4,838,408 - Pf6N2E2_4156 0.50 +0.2
4,838,408 - Pf6N2E2_4156 0.50 -0.0
4,838,408 - Pf6N2E2_4156 0.50 +0.1
4,838,408 - Pf6N2E2_4156 0.50 +0.3
4,838,408 - Pf6N2E2_4156 0.50 +0.6
4,838,408 - Pf6N2E2_4156 0.50 -1.0
4,838,408 - Pf6N2E2_4156 0.50 -1.7
4,838,437 + Pf6N2E2_4156 0.52 -1.5
4,838,437 + Pf6N2E2_4156 0.52 +0.5
4,838,577 + Pf6N2E2_4156 0.59 +1.4
4,838,578 - Pf6N2E2_4156 0.59 +0.4
4,838,578 - Pf6N2E2_4156 0.59 -0.1
4,839,186 + Pf6N2E2_4156 0.88 +0.1
4,839,387 - +0.3
4,839,397 + -0.3
4,839,397 + -0.1
4,839,397 + +0.6
4,839,397 + +0.1
4,839,398 - -0.3
4,839,398 - -0.4
4,839,398 - -0.0
4,839,398 - +0.3
4,839,398 - -1.6
4,839,398 - -0.2
4,839,753 + Pf6N2E2_4157 0.37 -0.5
4,839,754 - Pf6N2E2_4157 0.37 -0.7
4,839,967 - Pf6N2E2_4157 0.61 +0.5
4,839,967 - Pf6N2E2_4157 0.61 -0.7
4,839,967 - Pf6N2E2_4157 0.61 +0.8
4,840,017 + Pf6N2E2_4157 0.67 +0.6
4,840,017 + Pf6N2E2_4157 0.67 +0.5
4,840,152 + Pf6N2E2_4157 0.82 -0.1
4,840,152 + Pf6N2E2_4157 0.82 -0.4
4,840,152 + Pf6N2E2_4157 0.82 -1.4
4,840,153 - Pf6N2E2_4157 0.82 -0.3
4,840,153 - Pf6N2E2_4157 0.82 +0.6
4,840,323 + +0.1
4,840,323 + +0.9
4,840,323 + +0.8
4,840,323 + -0.0
4,840,323 + +0.9
4,840,324 - -0.2
4,840,324 - -0.5
4,840,324 - -0.1
4,840,324 - -0.8
4,840,324 - +0.2

Or see this region's nucleotide sequence