Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_400 and Pf6N2E2_401 are separated by 135 nucleotides Pf6N2E2_401 and Pf6N2E2_402 are separated by 97 nucleotides Pf6N2E2_402 and Pf6N2E2_403 are separated by 39 nucleotides
Pf6N2E2_400 - Ferrichrome-iron receptor, at 468,765 to 471,185
_400
Pf6N2E2_401 - Cyn operon transcriptional activator, at 471,321 to 472,220
_401
Pf6N2E2_402 - Carbonic anhydrase (EC 4.2.1.1), at 472,318 to 472,977
_402
Pf6N2E2_403 - Cyanate hydratase (EC 4.2.1.104), at 473,017 to 473,487
_403
Position (kb) 471
472
473 Strain fitness (log2 ratio) -1
0
1
2
3 at 470.460 kb on + strand, within Pf6N2E2_400 at 470.461 kb on - strand, within Pf6N2E2_400 at 470.461 kb on - strand, within Pf6N2E2_400 at 470.487 kb on + strand, within Pf6N2E2_400 at 470.514 kb on + strand, within Pf6N2E2_400 at 470.515 kb on - strand, within Pf6N2E2_400 at 470.527 kb on - strand, within Pf6N2E2_400 at 470.630 kb on - strand, within Pf6N2E2_400 at 470.719 kb on - strand, within Pf6N2E2_400 at 470.719 kb on - strand, within Pf6N2E2_400 at 470.721 kb on + strand, within Pf6N2E2_400 at 470.994 kb on + strand at 470.995 kb on - strand at 471.105 kb on + strand at 471.105 kb on + strand at 471.105 kb on + strand at 471.105 kb on + strand at 471.106 kb on - strand at 471.106 kb on - strand at 471.106 kb on - strand at 471.129 kb on + strand at 471.152 kb on + strand at 471.170 kb on + strand at 471.233 kb on + strand at 471.233 kb on + strand at 471.234 kb on - strand at 471.234 kb on - strand at 471.248 kb on + strand at 471.249 kb on - strand at 471.314 kb on - strand at 471.322 kb on + strand at 471.322 kb on + strand at 471.322 kb on + strand at 471.323 kb on - strand at 471.323 kb on - strand at 471.323 kb on - strand at 471.492 kb on + strand, within Pf6N2E2_401 at 471.493 kb on - strand, within Pf6N2E2_401 at 471.493 kb on - strand, within Pf6N2E2_401 at 471.501 kb on + strand, within Pf6N2E2_401 at 471.502 kb on - strand, within Pf6N2E2_401 at 471.590 kb on - strand, within Pf6N2E2_401 at 471.590 kb on - strand, within Pf6N2E2_401 at 471.755 kb on + strand, within Pf6N2E2_401 at 471.756 kb on - strand, within Pf6N2E2_401 at 471.759 kb on + strand, within Pf6N2E2_401 at 471.854 kb on + strand, within Pf6N2E2_401 at 471.855 kb on - strand, within Pf6N2E2_401 at 471.898 kb on + strand, within Pf6N2E2_401 at 471.898 kb on + strand, within Pf6N2E2_401 at 471.898 kb on + strand, within Pf6N2E2_401 at 471.899 kb on - strand, within Pf6N2E2_401 at 471.899 kb on - strand, within Pf6N2E2_401 at 471.994 kb on + strand, within Pf6N2E2_401 at 472.009 kb on + strand, within Pf6N2E2_401 at 472.018 kb on + strand, within Pf6N2E2_401 at 472.018 kb on + strand, within Pf6N2E2_401 at 472.018 kb on + strand, within Pf6N2E2_401 at 472.019 kb on - strand, within Pf6N2E2_401 at 472.019 kb on - strand, within Pf6N2E2_401 at 472.019 kb on - strand, within Pf6N2E2_401 at 472.186 kb on + strand at 472.187 kb on - strand at 472.187 kb on - strand at 472.279 kb on + strand at 472.279 kb on + strand at 472.279 kb on + strand at 472.279 kb on + strand at 472.280 kb on - strand at 472.280 kb on - strand at 472.280 kb on - strand at 472.601 kb on + strand, within Pf6N2E2_402 at 472.602 kb on - strand, within Pf6N2E2_402 at 472.751 kb on + strand, within Pf6N2E2_402 at 472.861 kb on + strand, within Pf6N2E2_402 at 472.861 kb on + strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.985 kb on - strand at 473.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 470,460 + Pf6N2E2_400 0.70 -0.5 470,461 - Pf6N2E2_400 0.70 +0.7 470,461 - Pf6N2E2_400 0.70 +0.5 470,487 + Pf6N2E2_400 0.71 +0.1 470,514 + Pf6N2E2_400 0.72 +1.3 470,515 - Pf6N2E2_400 0.72 -1.2 470,527 - Pf6N2E2_400 0.73 +0.7 470,630 - Pf6N2E2_400 0.77 +1.4 470,719 - Pf6N2E2_400 0.81 -1.5 470,719 - Pf6N2E2_400 0.81 -1.1 470,721 + Pf6N2E2_400 0.81 -0.2 470,994 + -1.1 470,995 - -0.8 471,105 + -1.3 471,105 + -0.2 471,105 + -0.1 471,105 + +0.6 471,106 - +0.9 471,106 - +0.1 471,106 - +0.9 471,129 + +0.2 471,152 + -1.2 471,170 + -1.6 471,233 + +0.3 471,233 + +0.2 471,234 - +0.8 471,234 - -0.5 471,248 + -1.1 471,249 - +1.0 471,314 - +1.4 471,322 + +3.1 471,322 + +0.1 471,322 + +0.8 471,323 - +2.0 471,323 - -1.4 471,323 - +1.4 471,492 + Pf6N2E2_401 0.19 +0.5 471,493 - Pf6N2E2_401 0.19 +0.8 471,493 - Pf6N2E2_401 0.19 -0.9 471,501 + Pf6N2E2_401 0.20 +1.1 471,502 - Pf6N2E2_401 0.20 +0.2 471,590 - Pf6N2E2_401 0.30 +2.7 471,590 - Pf6N2E2_401 0.30 -1.0 471,755 + Pf6N2E2_401 0.48 +0.6 471,756 - Pf6N2E2_401 0.48 -0.2 471,759 + Pf6N2E2_401 0.49 +0.6 471,854 + Pf6N2E2_401 0.59 +0.8 471,855 - Pf6N2E2_401 0.59 +1.3 471,898 + Pf6N2E2_401 0.64 +0.3 471,898 + Pf6N2E2_401 0.64 +1.0 471,898 + Pf6N2E2_401 0.64 +0.6 471,899 - Pf6N2E2_401 0.64 +1.3 471,899 - Pf6N2E2_401 0.64 +0.5 471,994 + Pf6N2E2_401 0.75 -0.4 472,009 + Pf6N2E2_401 0.76 -1.0 472,018 + Pf6N2E2_401 0.77 +1.2 472,018 + Pf6N2E2_401 0.77 -0.2 472,018 + Pf6N2E2_401 0.77 +1.5 472,019 - Pf6N2E2_401 0.78 -0.7 472,019 - Pf6N2E2_401 0.78 +0.3 472,019 - Pf6N2E2_401 0.78 +0.8 472,186 + +1.5 472,187 - +0.1 472,187 - +0.2 472,279 + -0.9 472,279 + +2.9 472,279 + +0.3 472,279 + +0.2 472,280 - +0.3 472,280 - -1.5 472,280 - -1.1 472,601 + Pf6N2E2_402 0.43 -1.1 472,602 - Pf6N2E2_402 0.43 +0.0 472,751 + Pf6N2E2_402 0.66 +0.6 472,861 + Pf6N2E2_402 0.82 -0.5 472,861 + Pf6N2E2_402 0.82 +0.5 472,862 - Pf6N2E2_402 0.82 +0.8 472,862 - Pf6N2E2_402 0.82 +0.6 472,862 - Pf6N2E2_402 0.82 +0.3 472,862 - Pf6N2E2_402 0.82 +0.3 472,862 - Pf6N2E2_402 0.82 -0.9 472,985 - +1.2 473,012 - +0.2
Or see this region's nucleotide sequence