Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4008 and Pf6N2E2_4009 are separated by 235 nucleotides Pf6N2E2_4009 and Pf6N2E2_4010 are separated by 146 nucleotides
Pf6N2E2_4008 - hypothetical protein, at 4,677,921 to 4,678,304
_4008
Pf6N2E2_4009 - GGDEF domain protein, at 4,678,540 to 4,680,447
_4009
Pf6N2E2_4010 - Fatty acid desaturase (EC 1.14.19.1); Delta-9 fatty acid desaturase (EC 1.14.19.1), at 4,680,594 to 4,681,778
_4010
Position (kb)
4678
4679
4680
4681 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4677.649 kb on + strand at 4677.649 kb on + strand at 4677.649 kb on + strand at 4677.649 kb on + strand at 4677.649 kb on + strand at 4677.649 kb on + strand at 4677.650 kb on - strand at 4677.650 kb on - strand at 4677.650 kb on - strand at 4677.660 kb on + strand at 4677.660 kb on + strand at 4677.660 kb on + strand at 4677.660 kb on + strand at 4677.661 kb on - strand at 4677.672 kb on + strand at 4677.672 kb on + strand at 4677.673 kb on - strand at 4677.739 kb on + strand at 4677.740 kb on - strand at 4677.740 kb on - strand at 4677.751 kb on + strand at 4677.777 kb on - strand at 4677.791 kb on + strand at 4677.854 kb on + strand at 4677.854 kb on + strand at 4677.854 kb on + strand at 4677.854 kb on + strand at 4677.854 kb on + strand at 4677.854 kb on + strand at 4677.854 kb on + strand at 4677.855 kb on - strand at 4677.855 kb on - strand at 4677.855 kb on - strand at 4677.855 kb on - strand at 4677.855 kb on - strand at 4677.855 kb on - strand at 4677.855 kb on - strand at 4677.855 kb on - strand at 4677.855 kb on - strand at 4677.858 kb on + strand at 4677.858 kb on + strand at 4677.858 kb on + strand at 4677.858 kb on + strand at 4677.859 kb on - strand at 4677.859 kb on - strand at 4677.859 kb on - strand at 4677.859 kb on - strand at 4677.859 kb on - strand at 4677.859 kb on - strand at 4677.859 kb on - strand at 4677.859 kb on - strand at 4677.859 kb on - strand at 4677.911 kb on + strand at 4677.912 kb on - strand at 4677.912 kb on - strand at 4677.915 kb on + strand at 4677.915 kb on + strand at 4677.915 kb on + strand at 4677.915 kb on + strand at 4677.915 kb on + strand at 4677.915 kb on + strand at 4677.915 kb on + strand at 4677.915 kb on + strand at 4677.915 kb on + strand at 4677.915 kb on + strand at 4677.915 kb on + strand at 4677.915 kb on + strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.916 kb on - strand at 4677.920 kb on + strand at 4677.920 kb on + strand at 4677.920 kb on + strand at 4677.921 kb on - strand at 4677.921 kb on - strand at 4677.921 kb on - strand at 4677.921 kb on - strand at 4677.921 kb on - strand at 4677.921 kb on - strand at 4677.921 kb on - strand at 4677.921 kb on - strand at 4678.023 kb on + strand, within Pf6N2E2_4008 at 4678.023 kb on + strand, within Pf6N2E2_4008 at 4678.023 kb on + strand, within Pf6N2E2_4008 at 4678.026 kb on + strand, within Pf6N2E2_4008 at 4678.026 kb on + strand, within Pf6N2E2_4008 at 4678.026 kb on + strand, within Pf6N2E2_4008 at 4678.027 kb on - strand, within Pf6N2E2_4008 at 4678.027 kb on - strand, within Pf6N2E2_4008 at 4678.027 kb on - strand, within Pf6N2E2_4008 at 4678.027 kb on - strand, within Pf6N2E2_4008 at 4678.097 kb on - strand, within Pf6N2E2_4008 at 4678.097 kb on - strand, within Pf6N2E2_4008 at 4678.097 kb on - strand, within Pf6N2E2_4008 at 4678.097 kb on - strand, within Pf6N2E2_4008 at 4678.507 kb on + strand at 4678.507 kb on + strand at 4678.508 kb on - strand at 4678.508 kb on - strand at 4678.508 kb on - strand at 4678.508 kb on - strand at 4678.511 kb on + strand at 4678.512 kb on - strand at 4678.512 kb on - strand at 4678.512 kb on - strand at 4678.512 kb on - strand at 4678.512 kb on - strand at 4678.512 kb on - strand at 4678.695 kb on + strand at 4678.696 kb on - strand at 4678.804 kb on - strand, within Pf6N2E2_4009 at 4678.991 kb on + strand, within Pf6N2E2_4009 at 4678.991 kb on + strand, within Pf6N2E2_4009 at 4678.991 kb on + strand, within Pf6N2E2_4009 at 4678.991 kb on + strand, within Pf6N2E2_4009 at 4678.991 kb on + strand, within Pf6N2E2_4009 at 4678.991 kb on + strand, within Pf6N2E2_4009 at 4678.991 kb on + strand, within Pf6N2E2_4009 at 4678.991 kb on + strand, within Pf6N2E2_4009 at 4678.991 kb on + strand, within Pf6N2E2_4009 at 4678.992 kb on - strand, within Pf6N2E2_4009 at 4678.992 kb on - strand, within Pf6N2E2_4009 at 4678.992 kb on - strand, within Pf6N2E2_4009 at 4678.992 kb on - strand, within Pf6N2E2_4009 at 4678.992 kb on - strand, within Pf6N2E2_4009 at 4679.023 kb on + strand, within Pf6N2E2_4009 at 4679.023 kb on + strand, within Pf6N2E2_4009 at 4679.023 kb on + strand, within Pf6N2E2_4009 at 4679.131 kb on - strand, within Pf6N2E2_4009 at 4679.131 kb on - strand, within Pf6N2E2_4009 at 4679.342 kb on + strand, within Pf6N2E2_4009 at 4679.342 kb on + strand, within Pf6N2E2_4009 at 4679.343 kb on - strand, within Pf6N2E2_4009 at 4679.512 kb on + strand, within Pf6N2E2_4009 at 4679.512 kb on + strand, within Pf6N2E2_4009 at 4679.513 kb on - strand, within Pf6N2E2_4009 at 4679.513 kb on - strand, within Pf6N2E2_4009 at 4679.513 kb on - strand, within Pf6N2E2_4009 at 4679.831 kb on + strand, within Pf6N2E2_4009 at 4679.831 kb on + strand, within Pf6N2E2_4009 at 4679.831 kb on + strand, within Pf6N2E2_4009 at 4679.889 kb on - strand, within Pf6N2E2_4009 at 4679.889 kb on - strand, within Pf6N2E2_4009 at 4679.889 kb on - strand, within Pf6N2E2_4009 at 4680.012 kb on + strand, within Pf6N2E2_4009 at 4680.012 kb on + strand, within Pf6N2E2_4009 at 4680.013 kb on - strand, within Pf6N2E2_4009 at 4680.013 kb on - strand, within Pf6N2E2_4009 at 4680.013 kb on - strand, within Pf6N2E2_4009 at 4680.026 kb on + strand, within Pf6N2E2_4009 at 4680.026 kb on + strand, within Pf6N2E2_4009 at 4680.361 kb on + strand at 4680.361 kb on + strand at 4680.404 kb on + strand at 4680.404 kb on + strand at 4680.405 kb on - strand at 4680.405 kb on - strand at 4680.405 kb on - strand at 4680.405 kb on - strand at 4680.405 kb on - strand at 4680.437 kb on + strand at 4680.437 kb on + strand at 4680.438 kb on - strand at 4680.438 kb on - strand at 4680.438 kb on - strand at 4680.483 kb on - strand at 4680.484 kb on + strand at 4680.484 kb on + strand at 4680.484 kb on + strand at 4680.484 kb on + strand at 4680.484 kb on + strand at 4680.484 kb on + strand at 4680.485 kb on - strand at 4680.485 kb on - strand at 4680.485 kb on - strand at 4680.485 kb on - strand at 4680.510 kb on + strand at 4680.510 kb on + strand at 4680.510 kb on + strand at 4680.510 kb on + strand at 4680.510 kb on + strand at 4680.511 kb on - strand at 4680.532 kb on + strand at 4680.532 kb on + strand at 4680.532 kb on + strand at 4680.532 kb on + strand at 4680.533 kb on - strand at 4680.533 kb on - strand at 4680.533 kb on - strand at 4680.551 kb on - strand at 4680.568 kb on + strand at 4680.568 kb on + strand at 4680.587 kb on - strand at 4680.593 kb on + strand at 4680.593 kb on + strand at 4680.593 kb on + strand at 4680.593 kb on + strand at 4680.593 kb on + strand at 4680.594 kb on - strand at 4680.594 kb on - strand at 4680.594 kb on - strand at 4680.594 kb on - strand at 4680.594 kb on - strand at 4680.594 kb on - strand at 4680.594 kb on - strand at 4680.594 kb on - strand at 4680.594 kb on - strand at 4680.600 kb on + strand at 4680.600 kb on + strand at 4680.601 kb on - strand at 4680.687 kb on + strand at 4680.687 kb on + strand at 4680.688 kb on - strand at 4680.688 kb on - strand at 4680.688 kb on - strand at 4680.901 kb on - strand, within Pf6N2E2_4010 at 4680.940 kb on - strand, within Pf6N2E2_4010 at 4680.981 kb on + strand, within Pf6N2E2_4010 at 4680.982 kb on - strand, within Pf6N2E2_4010 at 4680.982 kb on - strand, within Pf6N2E2_4010 at 4680.982 kb on - strand, within Pf6N2E2_4010 at 4681.059 kb on - strand, within Pf6N2E2_4010 at 4681.059 kb on - strand, within Pf6N2E2_4010 at 4681.059 kb on - strand, within Pf6N2E2_4010 at 4681.059 kb on - strand, within Pf6N2E2_4010 at 4681.228 kb on - strand, within Pf6N2E2_4010
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,677,649 + +0.1 4,677,649 + +1.7 4,677,649 + +1.0 4,677,649 + +1.4 4,677,649 + +0.1 4,677,649 + -0.2 4,677,650 - -0.1 4,677,650 - -0.1 4,677,650 - -0.4 4,677,660 + +0.6 4,677,660 + +0.5 4,677,660 + -0.4 4,677,660 + +0.7 4,677,661 - +1.3 4,677,672 + +0.7 4,677,672 + -0.7 4,677,673 - -2.2 4,677,739 + +0.4 4,677,740 - -0.6 4,677,740 - +0.3 4,677,751 + +0.9 4,677,777 - +0.7 4,677,791 + -0.1 4,677,854 + -0.9 4,677,854 + +0.6 4,677,854 + +1.1 4,677,854 + -0.1 4,677,854 + +0.9 4,677,854 + -1.3 4,677,854 + +0.1 4,677,855 - +0.6 4,677,855 - +0.1 4,677,855 - +0.6 4,677,855 - +1.9 4,677,855 - +0.3 4,677,855 - +0.9 4,677,855 - +0.4 4,677,855 - +0.3 4,677,855 - -0.0 4,677,858 + +0.2 4,677,858 + +1.1 4,677,858 + +0.1 4,677,858 + -0.1 4,677,859 - +0.3 4,677,859 - -0.5 4,677,859 - +0.2 4,677,859 - -1.0 4,677,859 - +0.2 4,677,859 - -0.2 4,677,859 - +0.4 4,677,859 - -0.7 4,677,859 - +1.2 4,677,911 + +0.7 4,677,912 - -1.8 4,677,912 - -0.3 4,677,915 + -0.4 4,677,915 + +1.0 4,677,915 + -0.0 4,677,915 + +0.6 4,677,915 + +0.9 4,677,915 + +0.2 4,677,915 + -0.6 4,677,915 + -0.7 4,677,915 + +0.7 4,677,915 + +0.9 4,677,915 + +0.2 4,677,915 + -0.7 4,677,916 - +0.4 4,677,916 - +0.1 4,677,916 - -0.0 4,677,916 - +1.3 4,677,916 - +0.2 4,677,916 - -0.4 4,677,916 - -0.5 4,677,916 - +1.4 4,677,916 - -0.3 4,677,916 - +0.5 4,677,916 - -0.3 4,677,916 - +0.2 4,677,916 - -0.5 4,677,916 - +0.4 4,677,916 - +1.0 4,677,916 - -0.9 4,677,916 - -0.4 4,677,916 - +0.2 4,677,916 - -1.2 4,677,916 - +0.2 4,677,920 + +0.7 4,677,920 + +1.1 4,677,920 + -0.2 4,677,921 - -3.1 4,677,921 - +0.2 4,677,921 - -1.5 4,677,921 - -0.8 4,677,921 - -0.4 4,677,921 - +0.0 4,677,921 - -0.3 4,677,921 - -0.6 4,678,023 + Pf6N2E2_4008 0.27 -0.3 4,678,023 + Pf6N2E2_4008 0.27 -0.9 4,678,023 + Pf6N2E2_4008 0.27 +0.1 4,678,026 + Pf6N2E2_4008 0.27 +0.1 4,678,026 + Pf6N2E2_4008 0.27 -0.3 4,678,026 + Pf6N2E2_4008 0.27 -0.1 4,678,027 - Pf6N2E2_4008 0.28 +1.8 4,678,027 - Pf6N2E2_4008 0.28 -0.5 4,678,027 - Pf6N2E2_4008 0.28 -0.1 4,678,027 - Pf6N2E2_4008 0.28 +0.6 4,678,097 - Pf6N2E2_4008 0.46 -3.6 4,678,097 - Pf6N2E2_4008 0.46 +0.3 4,678,097 - Pf6N2E2_4008 0.46 -0.8 4,678,097 - Pf6N2E2_4008 0.46 +0.4 4,678,507 + -0.8 4,678,507 + -0.0 4,678,508 - +0.1 4,678,508 - -0.1 4,678,508 - -0.3 4,678,508 - +0.8 4,678,511 + +0.3 4,678,512 - +0.2 4,678,512 - -0.5 4,678,512 - -0.7 4,678,512 - -0.4 4,678,512 - +0.7 4,678,512 - -0.5 4,678,695 + -1.8 4,678,696 - -0.1 4,678,804 - Pf6N2E2_4009 0.14 -3.3 4,678,991 + Pf6N2E2_4009 0.24 +0.7 4,678,991 + Pf6N2E2_4009 0.24 -0.5 4,678,991 + Pf6N2E2_4009 0.24 +0.1 4,678,991 + Pf6N2E2_4009 0.24 -0.7 4,678,991 + Pf6N2E2_4009 0.24 -3.6 4,678,991 + Pf6N2E2_4009 0.24 -4.4 4,678,991 + Pf6N2E2_4009 0.24 -0.2 4,678,991 + Pf6N2E2_4009 0.24 -4.1 4,678,991 + Pf6N2E2_4009 0.24 -0.6 4,678,992 - Pf6N2E2_4009 0.24 -0.6 4,678,992 - Pf6N2E2_4009 0.24 -0.4 4,678,992 - Pf6N2E2_4009 0.24 -1.1 4,678,992 - Pf6N2E2_4009 0.24 -0.4 4,678,992 - Pf6N2E2_4009 0.24 -0.7 4,679,023 + Pf6N2E2_4009 0.25 -1.0 4,679,023 + Pf6N2E2_4009 0.25 -1.4 4,679,023 + Pf6N2E2_4009 0.25 -2.8 4,679,131 - Pf6N2E2_4009 0.31 -1.2 4,679,131 - Pf6N2E2_4009 0.31 -0.4 4,679,342 + Pf6N2E2_4009 0.42 -0.8 4,679,342 + Pf6N2E2_4009 0.42 -0.4 4,679,343 - Pf6N2E2_4009 0.42 +1.2 4,679,512 + Pf6N2E2_4009 0.51 -0.6 4,679,512 + Pf6N2E2_4009 0.51 +0.4 4,679,513 - Pf6N2E2_4009 0.51 -0.2 4,679,513 - Pf6N2E2_4009 0.51 -0.3 4,679,513 - Pf6N2E2_4009 0.51 -1.3 4,679,831 + Pf6N2E2_4009 0.68 -1.4 4,679,831 + Pf6N2E2_4009 0.68 -0.7 4,679,831 + Pf6N2E2_4009 0.68 -0.4 4,679,889 - Pf6N2E2_4009 0.71 -0.9 4,679,889 - Pf6N2E2_4009 0.71 -1.5 4,679,889 - Pf6N2E2_4009 0.71 -2.8 4,680,012 + Pf6N2E2_4009 0.77 -0.6 4,680,012 + Pf6N2E2_4009 0.77 -0.4 4,680,013 - Pf6N2E2_4009 0.77 -0.2 4,680,013 - Pf6N2E2_4009 0.77 -1.9 4,680,013 - Pf6N2E2_4009 0.77 -0.3 4,680,026 + Pf6N2E2_4009 0.78 -1.2 4,680,026 + Pf6N2E2_4009 0.78 -0.9 4,680,361 + +0.6 4,680,361 + -0.9 4,680,404 + -1.0 4,680,404 + -1.4 4,680,405 - +1.0 4,680,405 - -0.9 4,680,405 - +0.5 4,680,405 - -0.1 4,680,405 - -1.4 4,680,437 + -0.6 4,680,437 + -1.4 4,680,438 - +1.1 4,680,438 - -0.4 4,680,438 - +0.3 4,680,483 - +0.4 4,680,484 + +0.8 4,680,484 + -0.1 4,680,484 + -1.0 4,680,484 + -1.1 4,680,484 + -0.3 4,680,484 + -0.2 4,680,485 - +0.4 4,680,485 - +0.6 4,680,485 - -0.2 4,680,485 - +0.2 4,680,510 + -0.8 4,680,510 + -1.5 4,680,510 + -1.0 4,680,510 + -1.0 4,680,510 + -0.8 4,680,511 - +1.1 4,680,532 + +0.3 4,680,532 + -1.8 4,680,532 + -0.5 4,680,532 + -2.8 4,680,533 - +1.2 4,680,533 - +0.8 4,680,533 - +0.6 4,680,551 - -0.2 4,680,568 + -2.2 4,680,568 + -3.6 4,680,587 - +0.9 4,680,593 + -0.7 4,680,593 + -0.6 4,680,593 + +0.3 4,680,593 + -0.4 4,680,593 + -0.1 4,680,594 - +0.7 4,680,594 - +0.8 4,680,594 - +0.1 4,680,594 - +0.2 4,680,594 - +2.1 4,680,594 - +0.6 4,680,594 - +0.7 4,680,594 - +0.6 4,680,594 - +0.4 4,680,600 + +1.1 4,680,600 + -0.8 4,680,601 - +0.8 4,680,687 + +0.3 4,680,687 + -0.2 4,680,688 - +0.3 4,680,688 - +0.5 4,680,688 - +1.0 4,680,901 - Pf6N2E2_4010 0.26 +0.5 4,680,940 - Pf6N2E2_4010 0.29 +1.1 4,680,981 + Pf6N2E2_4010 0.33 -0.8 4,680,982 - Pf6N2E2_4010 0.33 +0.4 4,680,982 - Pf6N2E2_4010 0.33 +0.2 4,680,982 - Pf6N2E2_4010 0.33 +1.1 4,681,059 - Pf6N2E2_4010 0.39 +0.9 4,681,059 - Pf6N2E2_4010 0.39 +0.9 4,681,059 - Pf6N2E2_4010 0.39 +1.2 4,681,059 - Pf6N2E2_4010 0.39 +1.7 4,681,228 - Pf6N2E2_4010 0.54 +0.9
Or see this region's nucleotide sequence