Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3764 and Pf6N2E2_3765 are separated by 11 nucleotides Pf6N2E2_3765 and Pf6N2E2_3766 are separated by 289 nucleotides Pf6N2E2_3766 and Pf6N2E2_3767 are separated by 181 nucleotides
Pf6N2E2_3764 - Thermonuclease family protein, at 4,385,926 to 4,386,738
_3764
Pf6N2E2_3765 - LSU ribosomal protein L31p @ LSU ribosomal protein L31p, zinc-dependent, at 4,386,750 to 4,386,974
_3765
Pf6N2E2_3766 - Helicase PriA essential for oriC/DnaA-independent DNA replication, at 4,387,264 to 4,389,483
_3766
Pf6N2E2_3767 - Arginyl-tRNA synthetase (EC 6.1.1.19), at 4,389,665 to 4,391,401
_3767
Position (kb)
4387
4388
4389
4390 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4386.377 kb on + strand, within Pf6N2E2_3764 at 4386.377 kb on + strand, within Pf6N2E2_3764 at 4386.377 kb on + strand, within Pf6N2E2_3764 at 4386.377 kb on + strand, within Pf6N2E2_3764 at 4386.378 kb on - strand, within Pf6N2E2_3764 at 4386.378 kb on - strand, within Pf6N2E2_3764 at 4386.378 kb on - strand, within Pf6N2E2_3764 at 4386.401 kb on + strand, within Pf6N2E2_3764 at 4386.402 kb on - strand, within Pf6N2E2_3764 at 4386.402 kb on - strand, within Pf6N2E2_3764 at 4386.575 kb on + strand, within Pf6N2E2_3764 at 4386.575 kb on + strand, within Pf6N2E2_3764 at 4386.575 kb on + strand, within Pf6N2E2_3764 at 4386.576 kb on - strand, within Pf6N2E2_3764 at 4386.576 kb on - strand, within Pf6N2E2_3764 at 4386.576 kb on - strand, within Pf6N2E2_3764 at 4386.576 kb on - strand, within Pf6N2E2_3764 at 4387.036 kb on - strand at 4387.036 kb on - strand at 4387.076 kb on - strand at 4387.076 kb on - strand at 4387.076 kb on - strand at 4387.076 kb on - strand at 4387.085 kb on - strand at 4387.099 kb on - strand at 4389.485 kb on + strand at 4389.485 kb on + strand at 4389.542 kb on + strand at 4389.542 kb on + strand at 4389.542 kb on + strand at 4389.542 kb on + strand at 4389.543 kb on - strand at 4389.543 kb on - strand at 4389.543 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,386,377 + Pf6N2E2_3764 0.55 +0.8 4,386,377 + Pf6N2E2_3764 0.55 +0.9 4,386,377 + Pf6N2E2_3764 0.55 +0.1 4,386,377 + Pf6N2E2_3764 0.55 +0.6 4,386,378 - Pf6N2E2_3764 0.56 +0.6 4,386,378 - Pf6N2E2_3764 0.56 +0.4 4,386,378 - Pf6N2E2_3764 0.56 -0.4 4,386,401 + Pf6N2E2_3764 0.58 +1.9 4,386,402 - Pf6N2E2_3764 0.59 +1.4 4,386,402 - Pf6N2E2_3764 0.59 -0.4 4,386,575 + Pf6N2E2_3764 0.80 +0.5 4,386,575 + Pf6N2E2_3764 0.80 +1.1 4,386,575 + Pf6N2E2_3764 0.80 +0.2 4,386,576 - Pf6N2E2_3764 0.80 +0.8 4,386,576 - Pf6N2E2_3764 0.80 +0.1 4,386,576 - Pf6N2E2_3764 0.80 +1.6 4,386,576 - Pf6N2E2_3764 0.80 -0.9 4,387,036 - +0.6 4,387,036 - +0.5 4,387,076 - +1.5 4,387,076 - +1.5 4,387,076 - +0.9 4,387,076 - +1.6 4,387,085 - -0.7 4,387,099 - +0.6 4,389,485 + -1.1 4,389,485 + -0.6 4,389,542 + +0.1 4,389,542 + +0.1 4,389,542 + -1.2 4,389,542 + -0.3 4,389,543 - -0.8 4,389,543 - +0.8 4,389,543 - -1.9
Or see this region's nucleotide sequence