Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3754 and Pf6N2E2_3755 are separated by 178 nucleotides Pf6N2E2_3755 and Pf6N2E2_3756 are separated by 31 nucleotides
Pf6N2E2_3754 - 3-dehydroquinate synthase (EC 4.2.3.4), at 4,374,837 to 4,375,937
_3754
Pf6N2E2_3755 - Shikimate kinase I (EC 2.7.1.71), at 4,376,116 to 4,376,607
_3755
Pf6N2E2_3756 - Type IV pilus biogenesis protein PilQ, at 4,376,639 to 4,378,729
_3756
Position (kb)
4376
4377 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4376.748 kb on + strand at 4376.776 kb on + strand at 4376.776 kb on + strand at 4376.776 kb on + strand at 4376.776 kb on + strand at 4376.776 kb on + strand at 4376.776 kb on + strand at 4376.777 kb on - strand at 4376.777 kb on - strand at 4376.777 kb on - strand at 4376.777 kb on - strand at 4376.780 kb on + strand at 4376.780 kb on + strand at 4376.780 kb on + strand at 4376.780 kb on + strand at 4376.780 kb on + strand at 4376.781 kb on - strand at 4376.781 kb on - strand at 4376.797 kb on + strand at 4377.161 kb on + strand, within Pf6N2E2_3756 at 4377.161 kb on + strand, within Pf6N2E2_3756 at 4377.161 kb on + strand, within Pf6N2E2_3756 at 4377.161 kb on + strand, within Pf6N2E2_3756 at 4377.162 kb on - strand, within Pf6N2E2_3756 at 4377.162 kb on - strand, within Pf6N2E2_3756 at 4377.162 kb on - strand, within Pf6N2E2_3756 at 4377.162 kb on - strand, within Pf6N2E2_3756 at 4377.199 kb on + strand, within Pf6N2E2_3756 at 4377.199 kb on + strand, within Pf6N2E2_3756 at 4377.355 kb on - strand, within Pf6N2E2_3756 at 4377.355 kb on - strand, within Pf6N2E2_3756 at 4377.355 kb on - strand, within Pf6N2E2_3756 at 4377.355 kb on - strand, within Pf6N2E2_3756 at 4377.355 kb on - strand, within Pf6N2E2_3756 at 4377.462 kb on + strand, within Pf6N2E2_3756 at 4377.462 kb on + strand, within Pf6N2E2_3756 at 4377.463 kb on - strand, within Pf6N2E2_3756 at 4377.576 kb on + strand, within Pf6N2E2_3756 at 4377.576 kb on + strand, within Pf6N2E2_3756 at 4377.576 kb on + strand, within Pf6N2E2_3756 at 4377.576 kb on + strand, within Pf6N2E2_3756 at 4377.576 kb on + strand, within Pf6N2E2_3756 at 4377.576 kb on + strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,376,748 + -0.4 4,376,776 + -0.9 4,376,776 + -0.5 4,376,776 + -1.1 4,376,776 + -0.5 4,376,776 + +0.5 4,376,776 + -0.8 4,376,777 - +1.8 4,376,777 - +1.2 4,376,777 - +0.3 4,376,777 - +2.3 4,376,780 + -2.5 4,376,780 + -0.0 4,376,780 + +1.8 4,376,780 + -3.4 4,376,780 + -1.9 4,376,781 - +0.9 4,376,781 - +1.0 4,376,797 + -0.4 4,377,161 + Pf6N2E2_3756 0.25 +0.2 4,377,161 + Pf6N2E2_3756 0.25 -0.2 4,377,161 + Pf6N2E2_3756 0.25 +1.1 4,377,161 + Pf6N2E2_3756 0.25 -0.4 4,377,162 - Pf6N2E2_3756 0.25 +0.5 4,377,162 - Pf6N2E2_3756 0.25 +0.7 4,377,162 - Pf6N2E2_3756 0.25 +0.6 4,377,162 - Pf6N2E2_3756 0.25 +1.1 4,377,199 + Pf6N2E2_3756 0.27 -0.4 4,377,199 + Pf6N2E2_3756 0.27 -0.1 4,377,355 - Pf6N2E2_3756 0.34 +0.9 4,377,355 - Pf6N2E2_3756 0.34 +0.6 4,377,355 - Pf6N2E2_3756 0.34 +1.0 4,377,355 - Pf6N2E2_3756 0.34 +0.4 4,377,355 - Pf6N2E2_3756 0.34 +1.1 4,377,462 + Pf6N2E2_3756 0.39 -0.2 4,377,462 + Pf6N2E2_3756 0.39 -1.3 4,377,463 - Pf6N2E2_3756 0.39 +1.2 4,377,576 + Pf6N2E2_3756 0.45 +0.9 4,377,576 + Pf6N2E2_3756 0.45 -0.6 4,377,576 + Pf6N2E2_3756 0.45 -0.6 4,377,576 + Pf6N2E2_3756 0.45 +0.5 4,377,576 + Pf6N2E2_3756 0.45 +0.8 4,377,576 + Pf6N2E2_3756 0.45 +0.6 4,377,577 - Pf6N2E2_3756 0.45 -0.5 4,377,577 - Pf6N2E2_3756 0.45 +0.6 4,377,577 - Pf6N2E2_3756 0.45 -0.0 4,377,577 - Pf6N2E2_3756 0.45 +1.5 4,377,577 - Pf6N2E2_3756 0.45 +0.6 4,377,577 - Pf6N2E2_3756 0.45 +1.2 4,377,577 - Pf6N2E2_3756 0.45 +0.9
Or see this region's nucleotide sequence