Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3674 and Pf6N2E2_3675 are separated by 11 nucleotides Pf6N2E2_3675 and Pf6N2E2_3676 are separated by 396 nucleotides
Pf6N2E2_3674 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 4,277,874 to 4,280,519
_3674
Pf6N2E2_3675 - Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12), at 4,280,531 to 4,282,168
_3675
Pf6N2E2_3676 - Phosphodiesterase/alkaline phosphatase D-like, at 4,282,565 to 4,283,956
_3676
Position (kb) 4280
4281
4282
4283 Strain fitness (log2 ratio) -1
0
1
2 at 4282.167 kb on - strand at 4282.231 kb on + strand at 4282.429 kb on - strand at 4282.429 kb on - strand at 4282.441 kb on - strand at 4282.441 kb on - strand at 4282.478 kb on + strand at 4282.478 kb on + strand at 4282.478 kb on + strand at 4282.479 kb on - strand at 4282.479 kb on - strand at 4282.482 kb on + strand at 4282.500 kb on - strand at 4282.564 kb on + strand at 4282.564 kb on + strand at 4282.565 kb on - strand at 4282.576 kb on + strand at 4282.577 kb on - strand at 4282.577 kb on - strand at 4282.690 kb on + strand at 4282.877 kb on + strand, within Pf6N2E2_3676 at 4282.878 kb on - strand, within Pf6N2E2_3676 at 4282.878 kb on - strand, within Pf6N2E2_3676 at 4282.878 kb on - strand, within Pf6N2E2_3676 at 4282.885 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.924 kb on + strand, within Pf6N2E2_3676 at 4282.924 kb on + strand, within Pf6N2E2_3676 at 4282.924 kb on + strand, within Pf6N2E2_3676 at 4282.925 kb on - strand, within Pf6N2E2_3676 at 4282.925 kb on - strand, within Pf6N2E2_3676 at 4282.925 kb on - strand, within Pf6N2E2_3676 at 4282.925 kb on - strand, within Pf6N2E2_3676 at 4282.944 kb on - strand, within Pf6N2E2_3676 at 4282.974 kb on + strand, within Pf6N2E2_3676 at 4282.975 kb on - strand, within Pf6N2E2_3676 at 4283.072 kb on + strand, within Pf6N2E2_3676 at 4283.073 kb on - strand, within Pf6N2E2_3676 at 4283.073 kb on - strand, within Pf6N2E2_3676 at 4283.073 kb on - strand, within Pf6N2E2_3676 at 4283.073 kb on - strand, within Pf6N2E2_3676
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,282,167 - +2.3 4,282,231 + +0.3 4,282,429 - +1.3 4,282,429 - +2.1 4,282,441 - +2.0 4,282,441 - +1.2 4,282,478 + +1.0 4,282,478 + +0.8 4,282,478 + +0.4 4,282,479 - -0.1 4,282,479 - +0.5 4,282,482 + +1.1 4,282,500 - +0.9 4,282,564 + +0.9 4,282,564 + +1.2 4,282,565 - +1.6 4,282,576 + +0.1 4,282,577 - -0.6 4,282,577 - -0.3 4,282,690 + -0.6 4,282,877 + Pf6N2E2_3676 0.22 -0.5 4,282,878 - Pf6N2E2_3676 0.22 +1.2 4,282,878 - Pf6N2E2_3676 0.22 +0.5 4,282,878 - Pf6N2E2_3676 0.22 +0.4 4,282,885 - Pf6N2E2_3676 0.23 +1.4 4,282,923 - Pf6N2E2_3676 0.26 -0.3 4,282,923 - Pf6N2E2_3676 0.26 +0.3 4,282,923 - Pf6N2E2_3676 0.26 -0.0 4,282,923 - Pf6N2E2_3676 0.26 +0.9 4,282,923 - Pf6N2E2_3676 0.26 +0.7 4,282,923 - Pf6N2E2_3676 0.26 +0.4 4,282,923 - Pf6N2E2_3676 0.26 +0.8 4,282,923 - Pf6N2E2_3676 0.26 +0.7 4,282,923 - Pf6N2E2_3676 0.26 +0.6 4,282,924 + Pf6N2E2_3676 0.26 +0.2 4,282,924 + Pf6N2E2_3676 0.26 -0.4 4,282,924 + Pf6N2E2_3676 0.26 -0.1 4,282,925 - Pf6N2E2_3676 0.26 -0.1 4,282,925 - Pf6N2E2_3676 0.26 +0.0 4,282,925 - Pf6N2E2_3676 0.26 +1.7 4,282,925 - Pf6N2E2_3676 0.26 +0.6 4,282,944 - Pf6N2E2_3676 0.27 +0.5 4,282,974 + Pf6N2E2_3676 0.29 +1.0 4,282,975 - Pf6N2E2_3676 0.29 +1.3 4,283,072 + Pf6N2E2_3676 0.36 +0.9 4,283,073 - Pf6N2E2_3676 0.36 +1.2 4,283,073 - Pf6N2E2_3676 0.36 +1.2 4,283,073 - Pf6N2E2_3676 0.36 -0.3 4,283,073 - Pf6N2E2_3676 0.36 -1.3
Or see this region's nucleotide sequence