Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3673

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3672 and Pf6N2E2_3673 are separated by 268 nucleotidesPf6N2E2_3673 and Pf6N2E2_3674 overlap by 6 nucleotides Pf6N2E2_3672 - Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42), at 4,274,540 to 4,277,491 _3672 Pf6N2E2_3673 - hypothetical protein, at 4,277,760 to 4,277,879 _3673 Pf6N2E2_3674 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 4,277,874 to 4,280,519 _3674 Position (kb) 4277 4278Strain fitness (log2 ratio) -1 0 1 2 3at 4277.022 kb on + strand, within Pf6N2E2_3672at 4277.022 kb on + strand, within Pf6N2E2_3672at 4277.023 kb on - strand, within Pf6N2E2_3672at 4277.023 kb on - strand, within Pf6N2E2_3672at 4277.023 kb on - strand, within Pf6N2E2_3672at 4277.023 kb on - strand, within Pf6N2E2_3672at 4277.034 kb on + strand, within Pf6N2E2_3672at 4277.240 kb on - strandat 4277.279 kb on + strandat 4277.330 kb on - strandat 4277.527 kb on + strandat 4277.543 kb on + strandat 4277.544 kb on - strandat 4277.549 kb on - strandat 4277.549 kb on - strandat 4277.717 kb on + strandat 4277.719 kb on + strandat 4277.719 kb on + strandat 4277.761 kb on + strandat 4278.225 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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4,277,022 + Pf6N2E2_3672 0.84 -0.1
4,277,022 + Pf6N2E2_3672 0.84 +1.3
4,277,023 - Pf6N2E2_3672 0.84 +3.7
4,277,023 - Pf6N2E2_3672 0.84 +3.2
4,277,023 - Pf6N2E2_3672 0.84 +3.0
4,277,023 - Pf6N2E2_3672 0.84 +2.4
4,277,034 + Pf6N2E2_3672 0.84 +3.0
4,277,240 - +2.9
4,277,279 + +0.2
4,277,330 - +2.8
4,277,527 + -1.5
4,277,543 + +0.5
4,277,544 - +1.9
4,277,549 - +2.6
4,277,549 - +3.2
4,277,717 + +0.7
4,277,719 + +1.9
4,277,719 + +1.5
4,277,761 + +1.6
4,278,225 + +0.4

Or see this region's nucleotide sequence