Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3661 and Pf6N2E2_3662 are separated by 192 nucleotides Pf6N2E2_3662 and Pf6N2E2_3663 are separated by 89 nucleotides Pf6N2E2_3663 and Pf6N2E2_3664 overlap by 8 nucleotides
Pf6N2E2_3661 - hypothetical protein, at 4,263,109 to 4,263,969
_3661
Pf6N2E2_3662 - hypothetical protein, at 4,264,162 to 4,264,302
_3662
Pf6N2E2_3663 - Glycosyl transferase, at 4,264,392 to 4,265,294
_3663
Pf6N2E2_3664 - Carbamoyltransferase in large core OS assembly cluster, at 4,265,287 to 4,267,035
_3664
Position (kb)
4264
4265
4266 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 4263.493 kb on + strand, within Pf6N2E2_3661 at 4263.493 kb on + strand, within Pf6N2E2_3661 at 4263.494 kb on - strand, within Pf6N2E2_3661 at 4263.494 kb on - strand, within Pf6N2E2_3661 at 4263.494 kb on - strand, within Pf6N2E2_3661 at 4263.563 kb on - strand, within Pf6N2E2_3661 at 4263.601 kb on + strand, within Pf6N2E2_3661 at 4263.601 kb on + strand, within Pf6N2E2_3661 at 4263.601 kb on + strand, within Pf6N2E2_3661 at 4263.602 kb on - strand, within Pf6N2E2_3661 at 4263.608 kb on + strand, within Pf6N2E2_3661 at 4263.609 kb on - strand, within Pf6N2E2_3661 at 4263.609 kb on - strand, within Pf6N2E2_3661 at 4263.609 kb on - strand, within Pf6N2E2_3661 at 4263.702 kb on - strand, within Pf6N2E2_3661 at 4263.720 kb on - strand, within Pf6N2E2_3661 at 4263.806 kb on + strand, within Pf6N2E2_3661 at 4263.962 kb on + strand at 4263.963 kb on - strand at 4263.995 kb on + strand at 4263.998 kb on - strand at 4263.998 kb on - strand at 4264.426 kb on + strand at 4264.427 kb on - strand at 4264.427 kb on - strand at 4264.475 kb on - strand at 4264.475 kb on - strand at 4264.495 kb on + strand, within Pf6N2E2_3663 at 4264.496 kb on - strand, within Pf6N2E2_3663 at 4264.667 kb on + strand, within Pf6N2E2_3663 at 4264.668 kb on - strand, within Pf6N2E2_3663 at 4264.668 kb on - strand, within Pf6N2E2_3663 at 4264.669 kb on + strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.768 kb on + strand, within Pf6N2E2_3663 at 4264.844 kb on - strand, within Pf6N2E2_3663 at 4264.941 kb on - strand, within Pf6N2E2_3663 at 4265.032 kb on + strand, within Pf6N2E2_3663 at 4265.032 kb on + strand, within Pf6N2E2_3663 at 4265.033 kb on - strand, within Pf6N2E2_3663 at 4265.033 kb on - strand, within Pf6N2E2_3663 at 4265.033 kb on - strand, within Pf6N2E2_3663 at 4265.033 kb on - strand, within Pf6N2E2_3663 at 4265.033 kb on - strand, within Pf6N2E2_3663 at 4265.033 kb on - strand, within Pf6N2E2_3663 at 4265.033 kb on - strand, within Pf6N2E2_3663 at 4265.033 kb on - strand, within Pf6N2E2_3663 at 4265.033 kb on - strand, within Pf6N2E2_3663 at 4265.033 kb on - strand, within Pf6N2E2_3663 at 4265.063 kb on + strand, within Pf6N2E2_3663 at 4265.252 kb on - strand at 4265.252 kb on - strand at 4265.255 kb on + strand at 4265.255 kb on + strand at 4265.256 kb on - strand at 4265.256 kb on - strand at 4265.257 kb on + strand at 4265.257 kb on + strand at 4265.257 kb on + strand at 4265.258 kb on - strand at 4265.258 kb on - strand at 4265.258 kb on - strand at 4265.258 kb on - strand at 4265.258 kb on - strand at 4265.295 kb on - strand at 4265.478 kb on - strand, within Pf6N2E2_3664 at 4265.481 kb on - strand, within Pf6N2E2_3664 at 4265.906 kb on + strand, within Pf6N2E2_3664 at 4265.922 kb on - strand, within Pf6N2E2_3664 at 4265.969 kb on + strand, within Pf6N2E2_3664 at 4265.969 kb on + strand, within Pf6N2E2_3664 at 4265.969 kb on + strand, within Pf6N2E2_3664 at 4265.970 kb on - strand, within Pf6N2E2_3664 at 4265.970 kb on - strand, within Pf6N2E2_3664 at 4265.970 kb on - strand, within Pf6N2E2_3664 at 4265.970 kb on - strand, within Pf6N2E2_3664 at 4265.970 kb on - strand, within Pf6N2E2_3664 at 4265.970 kb on - strand, within Pf6N2E2_3664 at 4265.970 kb on - strand, within Pf6N2E2_3664 at 4265.970 kb on - strand, within Pf6N2E2_3664 at 4265.970 kb on - strand, within Pf6N2E2_3664 at 4265.970 kb on - strand, within Pf6N2E2_3664 at 4266.131 kb on + strand, within Pf6N2E2_3664 at 4266.131 kb on + strand, within Pf6N2E2_3664 at 4266.132 kb on - strand, within Pf6N2E2_3664 at 4266.198 kb on - strand at 4266.198 kb on - strand, within Pf6N2E2_3664 at 4266.198 kb on - strand, within Pf6N2E2_3664 at 4266.198 kb on - strand, within Pf6N2E2_3664 at 4266.198 kb on - strand, within Pf6N2E2_3664 at 4266.264 kb on - strand, within Pf6N2E2_3664 at 4266.264 kb on - strand, within Pf6N2E2_3664 at 4266.264 kb on - strand, within Pf6N2E2_3664 at 4266.264 kb on - strand, within Pf6N2E2_3664 at 4266.285 kb on + strand, within Pf6N2E2_3664 at 4266.286 kb on - strand, within Pf6N2E2_3664 at 4266.286 kb on - strand, within Pf6N2E2_3664 at 4266.288 kb on - strand, within Pf6N2E2_3664
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,263,493 + Pf6N2E2_3661 0.45 +2.0 4,263,493 + Pf6N2E2_3661 0.45 +6.2 4,263,494 - Pf6N2E2_3661 0.45 +3.6 4,263,494 - Pf6N2E2_3661 0.45 +4.4 4,263,494 - Pf6N2E2_3661 0.45 +0.4 4,263,563 - Pf6N2E2_3661 0.53 +2.7 4,263,601 + Pf6N2E2_3661 0.57 +5.1 4,263,601 + Pf6N2E2_3661 0.57 +5.8 4,263,601 + Pf6N2E2_3661 0.57 +4.8 4,263,602 - Pf6N2E2_3661 0.57 +1.9 4,263,608 + Pf6N2E2_3661 0.58 +3.7 4,263,609 - Pf6N2E2_3661 0.58 +4.7 4,263,609 - Pf6N2E2_3661 0.58 +3.8 4,263,609 - Pf6N2E2_3661 0.58 +4.3 4,263,702 - Pf6N2E2_3661 0.69 +4.4 4,263,720 - Pf6N2E2_3661 0.71 +4.0 4,263,806 + Pf6N2E2_3661 0.81 +4.6 4,263,962 + +5.4 4,263,963 - +5.3 4,263,995 + +3.2 4,263,998 - +2.9 4,263,998 - +3.2 4,264,426 + +3.8 4,264,427 - +6.7 4,264,427 - +6.1 4,264,475 - +7.8 4,264,475 - +7.4 4,264,495 + Pf6N2E2_3663 0.11 +5.0 4,264,496 - Pf6N2E2_3663 0.12 +6.4 4,264,667 + Pf6N2E2_3663 0.30 +3.4 4,264,668 - Pf6N2E2_3663 0.31 +5.8 4,264,668 - Pf6N2E2_3663 0.31 +6.7 4,264,669 + Pf6N2E2_3663 0.31 +6.1 4,264,670 - Pf6N2E2_3663 0.31 +7.8 4,264,670 - Pf6N2E2_3663 0.31 +7.2 4,264,670 - Pf6N2E2_3663 0.31 +6.3 4,264,670 - Pf6N2E2_3663 0.31 +6.5 4,264,670 - Pf6N2E2_3663 0.31 +7.0 4,264,670 - Pf6N2E2_3663 0.31 +6.3 4,264,768 + Pf6N2E2_3663 0.42 +5.3 4,264,844 - Pf6N2E2_3663 0.50 +7.3 4,264,941 - Pf6N2E2_3663 0.61 +6.8 4,265,032 + Pf6N2E2_3663 0.71 +1.1 4,265,032 + Pf6N2E2_3663 0.71 +5.0 4,265,033 - Pf6N2E2_3663 0.71 +6.3 4,265,033 - Pf6N2E2_3663 0.71 +7.0 4,265,033 - Pf6N2E2_3663 0.71 +6.5 4,265,033 - Pf6N2E2_3663 0.71 +8.0 4,265,033 - Pf6N2E2_3663 0.71 +7.5 4,265,033 - Pf6N2E2_3663 0.71 +7.2 4,265,033 - Pf6N2E2_3663 0.71 +5.6 4,265,033 - Pf6N2E2_3663 0.71 +7.8 4,265,033 - Pf6N2E2_3663 0.71 +7.8 4,265,033 - Pf6N2E2_3663 0.71 +5.2 4,265,063 + Pf6N2E2_3663 0.74 +8.3 4,265,252 - +7.2 4,265,252 - +3.9 4,265,255 + +4.8 4,265,255 + +0.0 4,265,256 - +6.6 4,265,256 - +8.2 4,265,257 + +5.1 4,265,257 + +5.5 4,265,257 + +3.8 4,265,258 - +7.6 4,265,258 - +8.6 4,265,258 - +6.2 4,265,258 - +6.2 4,265,258 - +6.1 4,265,295 - +5.6 4,265,478 - Pf6N2E2_3664 0.11 -2.0 4,265,481 - Pf6N2E2_3664 0.11 -4.1 4,265,906 + Pf6N2E2_3664 0.35 +3.1 4,265,922 - Pf6N2E2_3664 0.36 +3.7 4,265,969 + Pf6N2E2_3664 0.39 +3.6 4,265,969 + Pf6N2E2_3664 0.39 +3.2 4,265,969 + Pf6N2E2_3664 0.39 +3.8 4,265,970 - Pf6N2E2_3664 0.39 +3.5 4,265,970 - Pf6N2E2_3664 0.39 +2.7 4,265,970 - Pf6N2E2_3664 0.39 +4.1 4,265,970 - Pf6N2E2_3664 0.39 -2.9 4,265,970 - Pf6N2E2_3664 0.39 +3.8 4,265,970 - Pf6N2E2_3664 0.39 +3.5 4,265,970 - Pf6N2E2_3664 0.39 +4.7 4,265,970 - Pf6N2E2_3664 0.39 +4.3 4,265,970 - Pf6N2E2_3664 0.39 +4.4 4,265,970 - Pf6N2E2_3664 0.39 +1.9 4,266,131 + Pf6N2E2_3664 0.48 -1.2 4,266,131 + Pf6N2E2_3664 0.48 -1.6 4,266,132 - Pf6N2E2_3664 0.48 +3.0 4,266,198 - +2.0 4,266,198 - Pf6N2E2_3664 0.52 +3.7 4,266,198 - Pf6N2E2_3664 0.52 +2.2 4,266,198 - Pf6N2E2_3664 0.52 +3.0 4,266,198 - Pf6N2E2_3664 0.52 +3.8 4,266,264 - Pf6N2E2_3664 0.56 +4.4 4,266,264 - Pf6N2E2_3664 0.56 +3.9 4,266,264 - Pf6N2E2_3664 0.56 +3.8 4,266,264 - Pf6N2E2_3664 0.56 +0.4 4,266,285 + Pf6N2E2_3664 0.57 +1.3 4,266,286 - Pf6N2E2_3664 0.57 +4.2 4,266,286 - Pf6N2E2_3664 0.57 +4.0 4,266,288 - Pf6N2E2_3664 0.57 +3.7
Or see this region's nucleotide sequence