Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3619 and Pf6N2E2_3620 are separated by 63 nucleotides Pf6N2E2_3620 and Pf6N2E2_3621 overlap by 19 nucleotides Pf6N2E2_3621 and Pf6N2E2_3622 are separated by 264 nucleotides
Pf6N2E2_3619 - NAD(P)HX epimerase / NAD(P)HX dehydratase, at 4,214,277 to 4,215,776
_3619
Pf6N2E2_3620 - Epoxyqueuosine (oQ) reductase QueG, at 4,215,840 to 4,216,919
_3620
Pf6N2E2_3621 - putative transporter, required for glycine utilization (from data) , at 4,216,901 to 4,217,512
_3621
Pf6N2E2_3622 - 3'-to-5' oligoribonuclease (orn), at 4,217,777 to 4,218,319
_3622
Position (kb)
4215
4216
4217 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4214.916 kb on + strand, within Pf6N2E2_3619 at 4214.916 kb on + strand, within Pf6N2E2_3619 at 4215.087 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.740 kb on - strand at 4215.740 kb on - strand at 4215.740 kb on - strand at 4215.740 kb on - strand at 4215.765 kb on - strand at 4215.765 kb on - strand at 4215.765 kb on - strand at 4215.808 kb on - strand at 4215.808 kb on - strand at 4215.808 kb on - strand at 4215.809 kb on + strand at 4215.809 kb on + strand at 4215.809 kb on + strand at 4215.810 kb on - strand at 4215.810 kb on - strand at 4215.810 kb on - strand at 4215.810 kb on - strand at 4215.810 kb on - strand at 4216.002 kb on + strand, within Pf6N2E2_3620 at 4216.002 kb on + strand, within Pf6N2E2_3620 at 4216.002 kb on + strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.087 kb on - strand, within Pf6N2E2_3620 at 4216.087 kb on - strand, within Pf6N2E2_3620 at 4216.087 kb on - strand, within Pf6N2E2_3620 at 4216.140 kb on + strand, within Pf6N2E2_3620 at 4216.140 kb on + strand, within Pf6N2E2_3620 at 4216.140 kb on + strand, within Pf6N2E2_3620 at 4216.141 kb on - strand, within Pf6N2E2_3620 at 4216.141 kb on - strand, within Pf6N2E2_3620 at 4216.141 kb on - strand, within Pf6N2E2_3620 at 4216.152 kb on + strand, within Pf6N2E2_3620 at 4216.170 kb on + strand, within Pf6N2E2_3620 at 4216.317 kb on - strand, within Pf6N2E2_3620 at 4216.353 kb on + strand, within Pf6N2E2_3620 at 4216.353 kb on + strand, within Pf6N2E2_3620 at 4216.353 kb on + strand, within Pf6N2E2_3620 at 4216.354 kb on - strand, within Pf6N2E2_3620 at 4216.457 kb on + strand, within Pf6N2E2_3620 at 4216.457 kb on + strand, within Pf6N2E2_3620 at 4216.457 kb on + strand, within Pf6N2E2_3620 at 4216.458 kb on - strand, within Pf6N2E2_3620 at 4216.479 kb on + strand, within Pf6N2E2_3620 at 4216.479 kb on + strand, within Pf6N2E2_3620 at 4216.480 kb on - strand, within Pf6N2E2_3620 at 4216.988 kb on - strand, within Pf6N2E2_3621 at 4216.988 kb on - strand, within Pf6N2E2_3621 at 4216.988 kb on - strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.070 kb on + strand, within Pf6N2E2_3621 at 4217.070 kb on + strand, within Pf6N2E2_3621 at 4217.070 kb on + strand, within Pf6N2E2_3621 at 4217.071 kb on - strand, within Pf6N2E2_3621 at 4217.071 kb on - strand, within Pf6N2E2_3621 at 4217.071 kb on - strand, within Pf6N2E2_3621 at 4217.099 kb on - strand, within Pf6N2E2_3621 at 4217.099 kb on - strand, within Pf6N2E2_3621 at 4217.321 kb on + strand, within Pf6N2E2_3621 at 4217.321 kb on + strand, within Pf6N2E2_3621 at 4217.322 kb on - strand, within Pf6N2E2_3621 at 4217.322 kb on - strand, within Pf6N2E2_3621 at 4217.322 kb on - strand, within Pf6N2E2_3621 at 4217.322 kb on - strand, within Pf6N2E2_3621 at 4217.326 kb on - strand, within Pf6N2E2_3621 at 4217.326 kb on - strand, within Pf6N2E2_3621 at 4217.496 kb on + strand at 4217.496 kb on + strand at 4217.496 kb on + strand at 4217.497 kb on - strand at 4217.598 kb on + strand at 4217.598 kb on + strand at 4217.598 kb on + strand at 4217.599 kb on - strand at 4217.599 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,214,916 + Pf6N2E2_3619 0.43 +1.0 4,214,916 + Pf6N2E2_3619 0.43 +1.2 4,215,087 - Pf6N2E2_3619 0.54 +0.5 4,215,132 - Pf6N2E2_3619 0.57 +0.0 4,215,132 - Pf6N2E2_3619 0.57 -1.5 4,215,132 - Pf6N2E2_3619 0.57 +0.4 4,215,132 - Pf6N2E2_3619 0.57 -1.6 4,215,132 - Pf6N2E2_3619 0.57 +0.1 4,215,132 - Pf6N2E2_3619 0.57 -0.3 4,215,350 - Pf6N2E2_3619 0.72 -0.4 4,215,350 - Pf6N2E2_3619 0.72 -0.8 4,215,350 - Pf6N2E2_3619 0.72 -0.7 4,215,350 - Pf6N2E2_3619 0.72 -0.1 4,215,350 - Pf6N2E2_3619 0.72 -0.6 4,215,350 - Pf6N2E2_3619 0.72 +1.1 4,215,350 - Pf6N2E2_3619 0.72 +0.2 4,215,350 - Pf6N2E2_3619 0.72 -0.4 4,215,740 - -0.5 4,215,740 - -0.7 4,215,740 - +2.0 4,215,740 - -0.3 4,215,765 - +0.9 4,215,765 - +0.5 4,215,765 - -1.1 4,215,808 - +0.5 4,215,808 - +1.4 4,215,808 - +0.5 4,215,809 + -0.9 4,215,809 + -0.6 4,215,809 + +0.8 4,215,810 - +0.5 4,215,810 - +0.3 4,215,810 - +0.7 4,215,810 - +0.4 4,215,810 - +0.6 4,216,002 + Pf6N2E2_3620 0.15 +1.1 4,216,002 + Pf6N2E2_3620 0.15 +1.3 4,216,002 + Pf6N2E2_3620 0.15 +2.0 4,216,003 - Pf6N2E2_3620 0.15 +2.3 4,216,003 - Pf6N2E2_3620 0.15 +1.6 4,216,003 - Pf6N2E2_3620 0.15 +2.0 4,216,003 - Pf6N2E2_3620 0.15 +1.1 4,216,087 - Pf6N2E2_3620 0.23 -0.0 4,216,087 - Pf6N2E2_3620 0.23 +1.2 4,216,087 - Pf6N2E2_3620 0.23 +2.5 4,216,140 + Pf6N2E2_3620 0.28 +0.6 4,216,140 + Pf6N2E2_3620 0.28 +2.2 4,216,140 + Pf6N2E2_3620 0.28 +1.6 4,216,141 - Pf6N2E2_3620 0.28 +0.8 4,216,141 - Pf6N2E2_3620 0.28 +1.1 4,216,141 - Pf6N2E2_3620 0.28 +1.5 4,216,152 + Pf6N2E2_3620 0.29 +0.4 4,216,170 + Pf6N2E2_3620 0.31 +0.1 4,216,317 - Pf6N2E2_3620 0.44 +0.8 4,216,353 + Pf6N2E2_3620 0.47 +1.1 4,216,353 + Pf6N2E2_3620 0.47 +0.6 4,216,353 + Pf6N2E2_3620 0.47 +1.6 4,216,354 - Pf6N2E2_3620 0.48 -1.1 4,216,457 + Pf6N2E2_3620 0.57 +1.6 4,216,457 + Pf6N2E2_3620 0.57 +2.9 4,216,457 + Pf6N2E2_3620 0.57 -0.3 4,216,458 - Pf6N2E2_3620 0.57 +0.2 4,216,479 + Pf6N2E2_3620 0.59 +1.1 4,216,479 + Pf6N2E2_3620 0.59 +1.7 4,216,480 - Pf6N2E2_3620 0.59 +0.7 4,216,988 - Pf6N2E2_3621 0.14 +0.2 4,216,988 - Pf6N2E2_3621 0.14 -0.2 4,216,988 - Pf6N2E2_3621 0.14 +0.3 4,217,037 + Pf6N2E2_3621 0.22 +0.0 4,217,037 + Pf6N2E2_3621 0.22 +0.0 4,217,037 + Pf6N2E2_3621 0.22 -0.9 4,217,037 + Pf6N2E2_3621 0.22 +0.8 4,217,037 + Pf6N2E2_3621 0.22 -0.6 4,217,037 + Pf6N2E2_3621 0.22 +0.2 4,217,037 + Pf6N2E2_3621 0.22 -0.5 4,217,070 + Pf6N2E2_3621 0.28 +0.2 4,217,070 + Pf6N2E2_3621 0.28 +0.3 4,217,070 + Pf6N2E2_3621 0.28 -0.0 4,217,071 - Pf6N2E2_3621 0.28 +0.9 4,217,071 - Pf6N2E2_3621 0.28 +0.6 4,217,071 - Pf6N2E2_3621 0.28 -0.0 4,217,099 - Pf6N2E2_3621 0.32 +0.0 4,217,099 - Pf6N2E2_3621 0.32 +1.1 4,217,321 + Pf6N2E2_3621 0.69 -0.1 4,217,321 + Pf6N2E2_3621 0.69 +1.0 4,217,322 - Pf6N2E2_3621 0.69 +1.0 4,217,322 - Pf6N2E2_3621 0.69 +1.0 4,217,322 - Pf6N2E2_3621 0.69 -0.8 4,217,322 - Pf6N2E2_3621 0.69 +0.9 4,217,326 - Pf6N2E2_3621 0.69 +0.0 4,217,326 - Pf6N2E2_3621 0.69 -0.3 4,217,496 + +1.1 4,217,496 + -1.2 4,217,496 + +0.1 4,217,497 - +0.0 4,217,598 + -0.2 4,217,598 + -2.1 4,217,598 + +0.0 4,217,599 - -1.5 4,217,599 - -0.3
Or see this region's nucleotide sequence