Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3509 and Pf6N2E2_3510 are separated by 236 nucleotides Pf6N2E2_3510 and Pf6N2E2_3511 are separated by 47 nucleotides Pf6N2E2_3511 and Pf6N2E2_3512 are separated by 17 nucleotides
Pf6N2E2_3509 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 4,090,187 to 4,092,133
_3509
Pf6N2E2_3510 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 4,092,370 to 4,092,822
_3510
Pf6N2E2_3511 - Biotin carboxyl carrier protein of acetyl-CoA carboxylase, at 4,092,870 to 4,093,334
_3511
Pf6N2E2_3512 - Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14), at 4,093,352 to 4,094,707
_3512
Position (kb)
4092
4093 Strain fitness (log2 ratio)
-1
0
1 at 4091.926 kb on - strand, within Pf6N2E2_3509 at 4092.016 kb on + strand at 4092.016 kb on + strand at 4092.016 kb on + strand at 4092.017 kb on - strand at 4092.017 kb on - strand at 4092.017 kb on - strand at 4092.285 kb on - strand at 4092.340 kb on + strand at 4092.354 kb on + strand at 4092.791 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,091,926 - Pf6N2E2_3509 0.89 +0.8 4,092,016 + -0.1 4,092,016 + +0.4 4,092,016 + -0.0 4,092,017 - -0.5 4,092,017 - +0.2 4,092,017 - -1.0 4,092,285 - +0.6 4,092,340 + +0.4 4,092,354 + -0.9 4,092,791 + +0.1
Or see this region's nucleotide sequence