Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3456 and Pf6N2E2_3457 overlap by 4 nucleotides Pf6N2E2_3457 and Pf6N2E2_3458 are separated by 139 nucleotides Pf6N2E2_3458 and Pf6N2E2_3459 are separated by 272 nucleotides
Pf6N2E2_3456 - Inner membrane protein YfiN, at 4,025,328 to 4,026,596
_3456
Pf6N2E2_3457 - Uncharacterized protein YfiR precursor, at 4,026,593 to 4,027,168
_3457
Pf6N2E2_3458 - Exodeoxyribonuclease V alpha chain (EC 3.1.11.5), at 4,027,308 to 4,027,979
_3458
Pf6N2E2_3459 - Exodeoxyribonuclease V alpha chain (EC 3.1.11.5), at 4,028,252 to 4,029,406
_3459
Position (kb)
4027
4028 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4026.314 kb on + strand, within Pf6N2E2_3456 at 4026.314 kb on + strand, within Pf6N2E2_3456 at 4026.315 kb on - strand, within Pf6N2E2_3456 at 4026.315 kb on - strand, within Pf6N2E2_3456 at 4026.418 kb on + strand, within Pf6N2E2_3456 at 4026.418 kb on + strand, within Pf6N2E2_3456 at 4026.419 kb on - strand, within Pf6N2E2_3456 at 4026.460 kb on + strand, within Pf6N2E2_3456 at 4026.460 kb on + strand, within Pf6N2E2_3456 at 4026.461 kb on - strand, within Pf6N2E2_3456 at 4026.599 kb on + strand at 4026.828 kb on + strand, within Pf6N2E2_3457 at 4026.828 kb on + strand, within Pf6N2E2_3457 at 4026.828 kb on + strand, within Pf6N2E2_3457 at 4026.881 kb on + strand, within Pf6N2E2_3457 at 4026.936 kb on + strand, within Pf6N2E2_3457 at 4026.996 kb on + strand, within Pf6N2E2_3457 at 4026.996 kb on + strand, within Pf6N2E2_3457 at 4026.996 kb on + strand, within Pf6N2E2_3457 at 4027.079 kb on + strand, within Pf6N2E2_3457 at 4027.080 kb on - strand, within Pf6N2E2_3457 at 4027.080 kb on - strand, within Pf6N2E2_3457 at 4027.119 kb on + strand at 4027.119 kb on + strand at 4027.120 kb on - strand at 4027.120 kb on - strand at 4027.120 kb on - strand at 4027.186 kb on + strand at 4027.192 kb on - strand at 4027.192 kb on - strand at 4027.250 kb on - strand at 4027.250 kb on - strand at 4027.250 kb on - strand at 4027.250 kb on - strand at 4027.250 kb on - strand at 4028.280 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,026,314 + Pf6N2E2_3456 0.78 +0.6 4,026,314 + Pf6N2E2_3456 0.78 +1.1 4,026,315 - Pf6N2E2_3456 0.78 +1.4 4,026,315 - Pf6N2E2_3456 0.78 -2.0 4,026,418 + Pf6N2E2_3456 0.86 +1.0 4,026,418 + Pf6N2E2_3456 0.86 -0.5 4,026,419 - Pf6N2E2_3456 0.86 -1.2 4,026,460 + Pf6N2E2_3456 0.89 +1.1 4,026,460 + Pf6N2E2_3456 0.89 +1.3 4,026,461 - Pf6N2E2_3456 0.89 -0.1 4,026,599 + -1.0 4,026,828 + Pf6N2E2_3457 0.41 +0.2 4,026,828 + Pf6N2E2_3457 0.41 +0.8 4,026,828 + Pf6N2E2_3457 0.41 -0.2 4,026,881 + Pf6N2E2_3457 0.50 +0.8 4,026,936 + Pf6N2E2_3457 0.60 +0.8 4,026,996 + Pf6N2E2_3457 0.70 +0.7 4,026,996 + Pf6N2E2_3457 0.70 +1.9 4,026,996 + Pf6N2E2_3457 0.70 +0.4 4,027,079 + Pf6N2E2_3457 0.84 +0.4 4,027,080 - Pf6N2E2_3457 0.85 -1.6 4,027,080 - Pf6N2E2_3457 0.85 -2.2 4,027,119 + +0.3 4,027,119 + +0.7 4,027,120 - -3.6 4,027,120 - -0.5 4,027,120 - -1.7 4,027,186 + -0.6 4,027,192 - -1.2 4,027,192 - -4.0 4,027,250 - -1.4 4,027,250 - -1.2 4,027,250 - +0.9 4,027,250 - -2.0 4,027,250 - -1.0 4,028,280 - -2.0
Or see this region's nucleotide sequence