Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3376 and Pf6N2E2_3377 are separated by 84 nucleotides Pf6N2E2_3377 and Pf6N2E2_3378 are separated by 10 nucleotides Pf6N2E2_3378 and Pf6N2E2_3379 are separated by 195 nucleotides
Pf6N2E2_3376 - Sodium-dependent transporter family protein, at 3,907,134 to 3,908,426
_3376
Pf6N2E2_3377 - hypothetical protein, at 3,908,511 to 3,908,645
_3377
Pf6N2E2_3378 - tmRNA-binding protein SmpB, at 3,908,656 to 3,909,138
_3378
Pf6N2E2_3379 - Lactate-responsive regulator LldR in Enterobacteria, GntR family, at 3,909,334 to 3,910,101
_3379
Position (kb)
3908
3909
3910 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.808 kb on - strand, within Pf6N2E2_3376 at 3907.808 kb on - strand, within Pf6N2E2_3376 at 3907.808 kb on - strand, within Pf6N2E2_3376 at 3907.808 kb on - strand, within Pf6N2E2_3376 at 3908.174 kb on - strand, within Pf6N2E2_3376 at 3908.174 kb on - strand, within Pf6N2E2_3376 at 3908.174 kb on - strand, within Pf6N2E2_3376 at 3908.174 kb on - strand, within Pf6N2E2_3376 at 3908.202 kb on - strand, within Pf6N2E2_3376 at 3908.202 kb on - strand, within Pf6N2E2_3376 at 3908.203 kb on + strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.290 kb on - strand, within Pf6N2E2_3376 at 3908.429 kb on - strand at 3908.429 kb on - strand at 3908.429 kb on - strand at 3908.445 kb on - strand at 3908.445 kb on - strand at 3908.445 kb on - strand at 3908.445 kb on - strand at 3908.445 kb on - strand at 3908.482 kb on - strand at 3908.482 kb on - strand at 3908.482 kb on - strand at 3908.641 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.473 kb on + strand, within Pf6N2E2_3379 at 3909.473 kb on + strand, within Pf6N2E2_3379 at 3909.473 kb on + strand, within Pf6N2E2_3379 at 3909.473 kb on + strand, within Pf6N2E2_3379 at 3909.473 kb on + strand, within Pf6N2E2_3379 at 3909.473 kb on + strand, within Pf6N2E2_3379 at 3909.474 kb on - strand, within Pf6N2E2_3379 at 3909.474 kb on - strand, within Pf6N2E2_3379 at 3909.474 kb on - strand, within Pf6N2E2_3379 at 3909.474 kb on - strand, within Pf6N2E2_3379 at 3909.527 kb on + strand, within Pf6N2E2_3379 at 3909.527 kb on + strand, within Pf6N2E2_3379 at 3909.527 kb on + strand, within Pf6N2E2_3379 at 3909.527 kb on + strand, within Pf6N2E2_3379 at 3909.528 kb on - strand, within Pf6N2E2_3379 at 3909.528 kb on - strand, within Pf6N2E2_3379 at 3909.528 kb on - strand, within Pf6N2E2_3379 at 3909.528 kb on - strand, within Pf6N2E2_3379 at 3909.528 kb on - strand, within Pf6N2E2_3379 at 3909.528 kb on - strand, within Pf6N2E2_3379 at 3909.528 kb on - strand, within Pf6N2E2_3379 at 3909.606 kb on + strand, within Pf6N2E2_3379 at 3909.690 kb on - strand, within Pf6N2E2_3379 at 3909.762 kb on - strand, within Pf6N2E2_3379 at 3909.762 kb on - strand, within Pf6N2E2_3379 at 3909.764 kb on + strand, within Pf6N2E2_3379 at 3909.764 kb on + strand, within Pf6N2E2_3379 at 3909.765 kb on - strand, within Pf6N2E2_3379 at 3909.765 kb on - strand, within Pf6N2E2_3379 at 3909.884 kb on + strand, within Pf6N2E2_3379 at 3909.884 kb on + strand, within Pf6N2E2_3379 at 3909.884 kb on + strand, within Pf6N2E2_3379 at 3909.884 kb on + strand, within Pf6N2E2_3379 at 3909.884 kb on + strand, within Pf6N2E2_3379 at 3909.884 kb on + strand, within Pf6N2E2_3379 at 3909.885 kb on - strand, within Pf6N2E2_3379 at 3909.885 kb on - strand, within Pf6N2E2_3379 at 3909.885 kb on - strand, within Pf6N2E2_3379 at 3909.885 kb on - strand, within Pf6N2E2_3379 at 3909.885 kb on - strand, within Pf6N2E2_3379 at 3909.885 kb on - strand, within Pf6N2E2_3379 at 3909.885 kb on - strand, within Pf6N2E2_3379 at 3909.885 kb on - strand, within Pf6N2E2_3379 at 3910.101 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,907,799 - Pf6N2E2_3376 0.51 +0.6 3,907,799 - Pf6N2E2_3376 0.51 -0.4 3,907,799 - Pf6N2E2_3376 0.51 -2.0 3,907,799 - Pf6N2E2_3376 0.51 -1.1 3,907,799 - Pf6N2E2_3376 0.51 -0.0 3,907,799 - Pf6N2E2_3376 0.51 -0.1 3,907,799 - Pf6N2E2_3376 0.51 -0.8 3,907,808 - Pf6N2E2_3376 0.52 +0.9 3,907,808 - Pf6N2E2_3376 0.52 +0.8 3,907,808 - Pf6N2E2_3376 0.52 +1.6 3,907,808 - Pf6N2E2_3376 0.52 -0.6 3,908,174 - Pf6N2E2_3376 0.80 +1.1 3,908,174 - Pf6N2E2_3376 0.80 -0.3 3,908,174 - Pf6N2E2_3376 0.80 -0.8 3,908,174 - Pf6N2E2_3376 0.80 +0.3 3,908,202 - Pf6N2E2_3376 0.83 +1.2 3,908,202 - Pf6N2E2_3376 0.83 +0.1 3,908,203 + Pf6N2E2_3376 0.83 -1.7 3,908,204 - Pf6N2E2_3376 0.83 -0.4 3,908,204 - Pf6N2E2_3376 0.83 -0.6 3,908,204 - Pf6N2E2_3376 0.83 -1.3 3,908,204 - Pf6N2E2_3376 0.83 +0.1 3,908,204 - Pf6N2E2_3376 0.83 +1.1 3,908,204 - Pf6N2E2_3376 0.83 -0.9 3,908,204 - Pf6N2E2_3376 0.83 +1.1 3,908,204 - Pf6N2E2_3376 0.83 +1.4 3,908,204 - Pf6N2E2_3376 0.83 -0.0 3,908,204 - Pf6N2E2_3376 0.83 +0.2 3,908,204 - Pf6N2E2_3376 0.83 -0.1 3,908,204 - Pf6N2E2_3376 0.83 -0.4 3,908,290 - Pf6N2E2_3376 0.89 -0.6 3,908,429 - -0.6 3,908,429 - -0.6 3,908,429 - +0.3 3,908,445 - +0.0 3,908,445 - -1.3 3,908,445 - -0.4 3,908,445 - -0.1 3,908,445 - -0.0 3,908,482 - -1.2 3,908,482 - -0.2 3,908,482 - -0.2 3,908,641 + +2.1 3,909,410 + -0.3 3,909,410 + +0.3 3,909,410 + +1.7 3,909,410 + +1.0 3,909,410 + +0.8 3,909,410 + -0.1 3,909,410 + +0.3 3,909,410 + +0.1 3,909,410 + -0.1 3,909,410 + +1.7 3,909,411 - Pf6N2E2_3379 0.10 -0.2 3,909,411 - Pf6N2E2_3379 0.10 +0.5 3,909,411 - Pf6N2E2_3379 0.10 +1.1 3,909,411 - Pf6N2E2_3379 0.10 +1.0 3,909,411 - Pf6N2E2_3379 0.10 +0.5 3,909,411 - Pf6N2E2_3379 0.10 -0.9 3,909,417 + Pf6N2E2_3379 0.11 -0.2 3,909,417 + Pf6N2E2_3379 0.11 -0.9 3,909,417 + Pf6N2E2_3379 0.11 +0.3 3,909,417 + Pf6N2E2_3379 0.11 -0.5 3,909,417 + Pf6N2E2_3379 0.11 +1.0 3,909,417 + Pf6N2E2_3379 0.11 +0.9 3,909,417 + Pf6N2E2_3379 0.11 +0.9 3,909,417 + Pf6N2E2_3379 0.11 -0.4 3,909,418 - Pf6N2E2_3379 0.11 +0.4 3,909,418 - Pf6N2E2_3379 0.11 +1.2 3,909,418 - Pf6N2E2_3379 0.11 +0.1 3,909,418 - Pf6N2E2_3379 0.11 -0.5 3,909,418 - Pf6N2E2_3379 0.11 +0.3 3,909,418 - Pf6N2E2_3379 0.11 -1.5 3,909,473 + Pf6N2E2_3379 0.18 -1.2 3,909,473 + Pf6N2E2_3379 0.18 -2.7 3,909,473 + Pf6N2E2_3379 0.18 -0.2 3,909,473 + Pf6N2E2_3379 0.18 +0.5 3,909,473 + Pf6N2E2_3379 0.18 +0.2 3,909,473 + Pf6N2E2_3379 0.18 -0.1 3,909,474 - Pf6N2E2_3379 0.18 -0.0 3,909,474 - Pf6N2E2_3379 0.18 +1.7 3,909,474 - Pf6N2E2_3379 0.18 -0.1 3,909,474 - Pf6N2E2_3379 0.18 -1.0 3,909,527 + Pf6N2E2_3379 0.25 -0.3 3,909,527 + Pf6N2E2_3379 0.25 -0.0 3,909,527 + Pf6N2E2_3379 0.25 +0.1 3,909,527 + Pf6N2E2_3379 0.25 +0.4 3,909,528 - Pf6N2E2_3379 0.25 +1.0 3,909,528 - Pf6N2E2_3379 0.25 +0.2 3,909,528 - Pf6N2E2_3379 0.25 +0.7 3,909,528 - Pf6N2E2_3379 0.25 +0.2 3,909,528 - Pf6N2E2_3379 0.25 +0.9 3,909,528 - Pf6N2E2_3379 0.25 +0.8 3,909,528 - Pf6N2E2_3379 0.25 +0.5 3,909,606 + Pf6N2E2_3379 0.35 -3.2 3,909,690 - Pf6N2E2_3379 0.46 +0.0 3,909,762 - Pf6N2E2_3379 0.56 +0.5 3,909,762 - Pf6N2E2_3379 0.56 +0.6 3,909,764 + Pf6N2E2_3379 0.56 -0.7 3,909,764 + Pf6N2E2_3379 0.56 +1.8 3,909,765 - Pf6N2E2_3379 0.56 -0.1 3,909,765 - Pf6N2E2_3379 0.56 +0.4 3,909,884 + Pf6N2E2_3379 0.72 -0.0 3,909,884 + Pf6N2E2_3379 0.72 -0.5 3,909,884 + Pf6N2E2_3379 0.72 -2.2 3,909,884 + Pf6N2E2_3379 0.72 +0.8 3,909,884 + Pf6N2E2_3379 0.72 +1.4 3,909,884 + Pf6N2E2_3379 0.72 -2.0 3,909,885 - Pf6N2E2_3379 0.72 +1.0 3,909,885 - Pf6N2E2_3379 0.72 +1.1 3,909,885 - Pf6N2E2_3379 0.72 -0.4 3,909,885 - Pf6N2E2_3379 0.72 +0.3 3,909,885 - Pf6N2E2_3379 0.72 +0.2 3,909,885 - Pf6N2E2_3379 0.72 -0.5 3,909,885 - Pf6N2E2_3379 0.72 +0.1 3,909,885 - Pf6N2E2_3379 0.72 +0.2 3,910,101 + -1.3
Or see this region's nucleotide sequence