Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3258 and Pf6N2E2_3259 overlap by 1 nucleotides Pf6N2E2_3259 and Pf6N2E2_3260 overlap by 1 nucleotides Pf6N2E2_3260 and Pf6N2E2_3261 are separated by 9 nucleotides Pf6N2E2_3261 and Pf6N2E2_3262 are separated by 136 nucleotides
Pf6N2E2_3258 - Uncharacterized ABC transporter, periplasmic component YrbD, at 3,767,426 to 3,767,893
_3258
Pf6N2E2_3259 - Uncharacterized ABC transporter, permease component YrbE, at 3,767,893 to 3,768,690
_3259
Pf6N2E2_3260 - Uncharacterized ABC transporter, ATP-binding protein YrbF, at 3,768,690 to 3,769,499
_3260
Pf6N2E2_3261 - hypothetical protein, at 3,769,509 to 3,769,637
_3261
Pf6N2E2_3262 - Arabinose 5-phosphate isomerase (EC 5.3.1.13), at 3,769,774 to 3,770,748
_3262
Position (kb)
3768
3769
3770 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3767.767 kb on - strand, within Pf6N2E2_3258 at 3767.773 kb on + strand, within Pf6N2E2_3258 at 3767.773 kb on + strand, within Pf6N2E2_3258 at 3767.775 kb on + strand, within Pf6N2E2_3258 at 3767.776 kb on - strand, within Pf6N2E2_3258 at 3767.776 kb on - strand, within Pf6N2E2_3258 at 3767.776 kb on - strand, within Pf6N2E2_3258 at 3767.779 kb on + strand, within Pf6N2E2_3258 at 3767.779 kb on + strand, within Pf6N2E2_3258 at 3767.779 kb on + strand, within Pf6N2E2_3258 at 3767.780 kb on - strand at 3768.014 kb on + strand, within Pf6N2E2_3259 at 3768.055 kb on + strand, within Pf6N2E2_3259 at 3768.137 kb on + strand, within Pf6N2E2_3259 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3769.481 kb on - strand at 3769.501 kb on + strand at 3769.502 kb on - strand at 3769.502 kb on - strand at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.591 kb on + strand, within Pf6N2E2_3261 at 3769.591 kb on + strand, within Pf6N2E2_3261 at 3769.592 kb on - strand, within Pf6N2E2_3261 at 3769.592 kb on - strand, within Pf6N2E2_3261 at 3769.592 kb on - strand, within Pf6N2E2_3261 at 3769.646 kb on + strand at 3769.666 kb on + strand at 3769.708 kb on + strand at 3769.708 kb on + strand at 3769.708 kb on + strand at 3769.708 kb on + strand at 3769.736 kb on + strand at 3769.761 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,767,767 - Pf6N2E2_3258 0.73 -2.8 3,767,773 + Pf6N2E2_3258 0.74 -2.1 3,767,773 + Pf6N2E2_3258 0.74 -2.4 3,767,775 + Pf6N2E2_3258 0.75 -3.9 3,767,776 - Pf6N2E2_3258 0.75 +0.0 3,767,776 - Pf6N2E2_3258 0.75 -1.4 3,767,776 - Pf6N2E2_3258 0.75 -2.1 3,767,779 + Pf6N2E2_3258 0.75 -1.8 3,767,779 + Pf6N2E2_3258 0.75 -3.8 3,767,779 + Pf6N2E2_3258 0.75 -1.7 3,767,780 - -0.8 3,768,014 + Pf6N2E2_3259 0.15 +0.2 3,768,055 + Pf6N2E2_3259 0.20 -0.8 3,768,137 + Pf6N2E2_3259 0.31 -2.9 3,768,853 + Pf6N2E2_3260 0.20 -1.8 3,768,853 + Pf6N2E2_3260 0.20 -3.8 3,768,853 + Pf6N2E2_3260 0.20 -2.8 3,768,853 + Pf6N2E2_3260 0.20 -0.8 3,768,853 + Pf6N2E2_3260 0.20 -2.3 3,768,853 + Pf6N2E2_3260 0.20 -1.3 3,768,853 + Pf6N2E2_3260 0.20 -0.8 3,768,853 + Pf6N2E2_3260 0.20 -2.7 3,768,853 + Pf6N2E2_3260 0.20 -3.9 3,768,853 + Pf6N2E2_3260 0.20 -2.6 3,768,857 + Pf6N2E2_3260 0.21 -2.3 3,768,857 + Pf6N2E2_3260 0.21 -2.6 3,768,857 + Pf6N2E2_3260 0.21 -2.8 3,768,857 + Pf6N2E2_3260 0.21 -2.1 3,768,857 + Pf6N2E2_3260 0.21 -2.1 3,768,857 + Pf6N2E2_3260 0.21 -2.1 3,768,857 + Pf6N2E2_3260 0.21 -3.3 3,768,857 + Pf6N2E2_3260 0.21 -2.9 3,768,857 + Pf6N2E2_3260 0.21 -3.3 3,768,857 + Pf6N2E2_3260 0.21 -2.1 3,769,481 - -3.3 3,769,501 + -2.7 3,769,502 - -2.1 3,769,502 - +0.1 3,769,590 - Pf6N2E2_3261 0.63 +1.7 3,769,590 - Pf6N2E2_3261 0.63 -0.6 3,769,590 - Pf6N2E2_3261 0.63 -0.3 3,769,590 - Pf6N2E2_3261 0.63 +1.2 3,769,590 - Pf6N2E2_3261 0.63 -1.6 3,769,590 - Pf6N2E2_3261 0.63 +1.2 3,769,590 - Pf6N2E2_3261 0.63 +0.5 3,769,591 + Pf6N2E2_3261 0.64 -1.3 3,769,591 + Pf6N2E2_3261 0.64 -0.8 3,769,592 - Pf6N2E2_3261 0.64 +1.2 3,769,592 - Pf6N2E2_3261 0.64 +1.3 3,769,592 - Pf6N2E2_3261 0.64 +1.0 3,769,646 + -0.8 3,769,666 + +0.4 3,769,708 + +3.5 3,769,708 + +2.4 3,769,708 + +1.7 3,769,708 + +3.0 3,769,736 + +4.0 3,769,761 + -0.6
Or see this region's nucleotide sequence