Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3254 and Pf6N2E2_3255 are separated by 22 nucleotides Pf6N2E2_3255 and Pf6N2E2_3256 are separated by 223 nucleotides Pf6N2E2_3256 and Pf6N2E2_3257 overlap by 4 nucleotides Pf6N2E2_3257 and Pf6N2E2_3258 are separated by 11 nucleotides Pf6N2E2_3258 and Pf6N2E2_3259 overlap by 1 nucleotides
Pf6N2E2_3254 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7), at 3,764,696 to 3,765,961
_3254
Pf6N2E2_3255 - YrbA protein, at 3,765,984 to 3,766,223
_3255
Pf6N2E2_3256 - Uncharacterized protein YrbB, at 3,766,447 to 3,766,764
_3256
Pf6N2E2_3257 - Uncharacterized ABC transporter, auxiliary component YrbC, at 3,766,761 to 3,767,414
_3257
Pf6N2E2_3258 - Uncharacterized ABC transporter, periplasmic component YrbD, at 3,767,426 to 3,767,893
_3258
Pf6N2E2_3259 - Uncharacterized ABC transporter, permease component YrbE, at 3,767,893 to 3,768,690
_3259
Position (kb)
3766
3767
3768 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.195 kb on - strand, within Pf6N2E2_3255 at 3766.195 kb on - strand, within Pf6N2E2_3255 at 3766.195 kb on - strand, within Pf6N2E2_3255 at 3766.213 kb on - strand at 3766.317 kb on + strand at 3766.489 kb on + strand, within Pf6N2E2_3256 at 3766.966 kb on + strand, within Pf6N2E2_3257 at 3766.978 kb on + strand, within Pf6N2E2_3257 at 3766.979 kb on - strand, within Pf6N2E2_3257 at 3767.074 kb on + strand, within Pf6N2E2_3257 at 3767.074 kb on + strand, within Pf6N2E2_3257 at 3767.075 kb on - strand, within Pf6N2E2_3257 at 3767.167 kb on + strand, within Pf6N2E2_3257 at 3767.167 kb on + strand, within Pf6N2E2_3257 at 3767.167 kb on + strand, within Pf6N2E2_3257 at 3767.168 kb on - strand, within Pf6N2E2_3257 at 3767.242 kb on + strand at 3767.243 kb on - strand, within Pf6N2E2_3257 at 3767.243 kb on - strand, within Pf6N2E2_3257 at 3767.243 kb on - strand, within Pf6N2E2_3257 at 3767.267 kb on - strand, within Pf6N2E2_3257 at 3767.406 kb on - strand at 3767.562 kb on + strand, within Pf6N2E2_3258 at 3767.562 kb on + strand at 3767.563 kb on - strand, within Pf6N2E2_3258 at 3767.563 kb on - strand, within Pf6N2E2_3258 at 3767.563 kb on - strand, within Pf6N2E2_3258 at 3767.563 kb on - strand, within Pf6N2E2_3258 at 3767.563 kb on - strand, within Pf6N2E2_3258 at 3767.594 kb on - strand, within Pf6N2E2_3258 at 3767.767 kb on - strand, within Pf6N2E2_3258 at 3767.773 kb on + strand, within Pf6N2E2_3258 at 3767.773 kb on + strand, within Pf6N2E2_3258 at 3767.775 kb on + strand, within Pf6N2E2_3258 at 3767.776 kb on - strand, within Pf6N2E2_3258 at 3767.776 kb on - strand, within Pf6N2E2_3258 at 3767.776 kb on - strand, within Pf6N2E2_3258 at 3767.779 kb on + strand, within Pf6N2E2_3258 at 3767.779 kb on + strand, within Pf6N2E2_3258 at 3767.779 kb on + strand, within Pf6N2E2_3258 at 3767.780 kb on - strand at 3768.014 kb on + strand, within Pf6N2E2_3259 at 3768.055 kb on + strand, within Pf6N2E2_3259 at 3768.137 kb on + strand, within Pf6N2E2_3259
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,766,076 - Pf6N2E2_3255 0.38 -1.8 3,766,076 - Pf6N2E2_3255 0.38 +1.5 3,766,076 - Pf6N2E2_3255 0.38 +1.7 3,766,076 - Pf6N2E2_3255 0.38 -2.1 3,766,076 - Pf6N2E2_3255 0.38 +1.1 3,766,076 - Pf6N2E2_3255 0.38 +0.0 3,766,195 - Pf6N2E2_3255 0.88 +0.4 3,766,195 - Pf6N2E2_3255 0.88 +1.2 3,766,195 - Pf6N2E2_3255 0.88 +0.6 3,766,213 - -0.2 3,766,317 + -2.9 3,766,489 + Pf6N2E2_3256 0.13 -2.9 3,766,966 + Pf6N2E2_3257 0.31 -3.5 3,766,978 + Pf6N2E2_3257 0.33 -2.9 3,766,979 - Pf6N2E2_3257 0.33 -0.8 3,767,074 + Pf6N2E2_3257 0.48 -1.8 3,767,074 + Pf6N2E2_3257 0.48 -1.6 3,767,075 - Pf6N2E2_3257 0.48 +0.1 3,767,167 + Pf6N2E2_3257 0.62 -1.4 3,767,167 + Pf6N2E2_3257 0.62 -1.4 3,767,167 + Pf6N2E2_3257 0.62 -2.1 3,767,168 - Pf6N2E2_3257 0.62 -3.2 3,767,242 + -0.8 3,767,243 - Pf6N2E2_3257 0.74 -2.4 3,767,243 - Pf6N2E2_3257 0.74 -3.6 3,767,243 - Pf6N2E2_3257 0.74 -2.4 3,767,267 - Pf6N2E2_3257 0.77 -2.8 3,767,406 - -0.8 3,767,562 + Pf6N2E2_3258 0.29 -1.8 3,767,562 + -1.4 3,767,563 - Pf6N2E2_3258 0.29 +0.2 3,767,563 - Pf6N2E2_3258 0.29 -3.4 3,767,563 - Pf6N2E2_3258 0.29 -1.4 3,767,563 - Pf6N2E2_3258 0.29 -2.9 3,767,563 - Pf6N2E2_3258 0.29 +0.2 3,767,594 - Pf6N2E2_3258 0.36 -2.1 3,767,767 - Pf6N2E2_3258 0.73 -2.8 3,767,773 + Pf6N2E2_3258 0.74 -2.1 3,767,773 + Pf6N2E2_3258 0.74 -2.4 3,767,775 + Pf6N2E2_3258 0.75 -3.9 3,767,776 - Pf6N2E2_3258 0.75 +0.0 3,767,776 - Pf6N2E2_3258 0.75 -1.4 3,767,776 - Pf6N2E2_3258 0.75 -2.1 3,767,779 + Pf6N2E2_3258 0.75 -1.8 3,767,779 + Pf6N2E2_3258 0.75 -3.8 3,767,779 + Pf6N2E2_3258 0.75 -1.7 3,767,780 - -0.8 3,768,014 + Pf6N2E2_3259 0.15 +0.2 3,768,055 + Pf6N2E2_3259 0.20 -0.8 3,768,137 + Pf6N2E2_3259 0.31 -2.9
Or see this region's nucleotide sequence