Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3163 and Pf6N2E2_3164 are separated by 667 nucleotides Pf6N2E2_3164 and Pf6N2E2_3165 are separated by 79 nucleotides
Pf6N2E2_3163 - putative DNA-binding protein, at 3,656,301 to 3,656,825
_3163
Pf6N2E2_3164 - GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2), at 3,657,493 to 3,659,070
_3164
Pf6N2E2_3165 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) / CBS domain, at 3,659,150 to 3,660,619
_3165
Position (kb)
3657
3658
3659
3660 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3656.548 kb on - strand, within Pf6N2E2_3163 at 3656.548 kb on - strand, within Pf6N2E2_3163 at 3656.548 kb on - strand, within Pf6N2E2_3163 at 3656.565 kb on + strand, within Pf6N2E2_3163 at 3656.583 kb on + strand, within Pf6N2E2_3163 at 3656.584 kb on - strand, within Pf6N2E2_3163 at 3656.584 kb on - strand, within Pf6N2E2_3163 at 3656.596 kb on - strand, within Pf6N2E2_3163 at 3656.648 kb on + strand, within Pf6N2E2_3163 at 3656.648 kb on + strand, within Pf6N2E2_3163 at 3656.648 kb on + strand, within Pf6N2E2_3163 at 3656.649 kb on - strand, within Pf6N2E2_3163 at 3656.800 kb on + strand at 3656.810 kb on + strand at 3656.811 kb on - strand at 3656.811 kb on - strand at 3656.817 kb on - strand at 3656.831 kb on + strand at 3656.831 kb on + strand at 3656.831 kb on + strand at 3656.831 kb on + strand at 3656.831 kb on + strand at 3656.832 kb on - strand at 3656.832 kb on - strand at 3656.832 kb on - strand at 3656.847 kb on - strand at 3656.847 kb on - strand at 3656.847 kb on - strand at 3656.988 kb on - strand at 3656.996 kb on + strand at 3656.997 kb on - strand at 3657.031 kb on + strand at 3657.031 kb on + strand at 3657.031 kb on + strand at 3657.031 kb on + strand at 3657.032 kb on - strand at 3657.032 kb on - strand at 3657.032 kb on - strand at 3657.032 kb on - strand at 3657.119 kb on + strand at 3657.120 kb on - strand at 3657.269 kb on + strand at 3657.270 kb on - strand at 3657.270 kb on - strand at 3657.270 kb on - strand at 3657.299 kb on + strand at 3657.305 kb on + strand at 3657.346 kb on + strand at 3657.346 kb on + strand at 3657.346 kb on + strand at 3657.346 kb on + strand at 3657.347 kb on - strand at 3657.347 kb on - strand at 3657.347 kb on - strand at 3657.347 kb on - strand at 3657.347 kb on - strand at 3657.347 kb on - strand at 3657.347 kb on - strand at 3657.347 kb on - strand at 3657.347 kb on - strand at 3657.392 kb on + strand at 3657.393 kb on - strand at 3657.418 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,656,548 - Pf6N2E2_3163 0.47 +0.8 3,656,548 - Pf6N2E2_3163 0.47 +0.1 3,656,548 - Pf6N2E2_3163 0.47 -1.1 3,656,565 + Pf6N2E2_3163 0.50 -1.0 3,656,583 + Pf6N2E2_3163 0.54 -0.1 3,656,584 - Pf6N2E2_3163 0.54 +0.2 3,656,584 - Pf6N2E2_3163 0.54 +0.2 3,656,596 - Pf6N2E2_3163 0.56 -1.1 3,656,648 + Pf6N2E2_3163 0.66 -0.9 3,656,648 + Pf6N2E2_3163 0.66 +1.1 3,656,648 + Pf6N2E2_3163 0.66 +1.6 3,656,649 - Pf6N2E2_3163 0.66 -0.2 3,656,800 + -1.3 3,656,810 + +0.5 3,656,811 - +0.4 3,656,811 - +0.9 3,656,817 - -0.3 3,656,831 + +0.2 3,656,831 + -1.2 3,656,831 + -0.3 3,656,831 + +1.3 3,656,831 + +0.2 3,656,832 - +0.3 3,656,832 - +1.1 3,656,832 - -1.3 3,656,847 - +1.6 3,656,847 - +2.1 3,656,847 - +0.3 3,656,988 - +0.2 3,656,996 + -0.1 3,656,997 - -0.7 3,657,031 + +1.4 3,657,031 + -0.3 3,657,031 + +0.2 3,657,031 + -1.8 3,657,032 - -0.4 3,657,032 - +0.2 3,657,032 - -0.2 3,657,032 - -1.6 3,657,119 + -0.9 3,657,120 - -0.1 3,657,269 + +0.9 3,657,270 - -0.6 3,657,270 - -0.2 3,657,270 - +0.8 3,657,299 + -0.0 3,657,305 + +0.1 3,657,346 + +1.1 3,657,346 + -0.6 3,657,346 + -0.1 3,657,346 + +0.1 3,657,347 - -0.0 3,657,347 - -0.6 3,657,347 - +2.4 3,657,347 - +0.2 3,657,347 - +1.5 3,657,347 - -0.6 3,657,347 - +0.3 3,657,347 - -0.2 3,657,347 - -0.0 3,657,392 + -0.1 3,657,393 - +0.1 3,657,418 + -0.3
Or see this region's nucleotide sequence