Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3143 and Pf6N2E2_3144 are separated by 132 nucleotides Pf6N2E2_3144 and Pf6N2E2_3145 are separated by 308 nucleotides
Pf6N2E2_3143 - Homoserine dehydrogenase (EC 1.1.1.3), at 3,636,167 to 3,637,471
_3143
Pf6N2E2_3144 - Thiol:disulfide interchange protein DsbC, at 3,637,604 to 3,638,335
_3144
Pf6N2E2_3145 - Tyrosine recombinase XerD, at 3,638,644 to 3,639,540
_3145
Position (kb)
3637
3638
3639 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3637.517 kb on - strand at 3637.879 kb on - strand, within Pf6N2E2_3144 at 3637.879 kb on - strand, within Pf6N2E2_3144 at 3637.879 kb on - strand, within Pf6N2E2_3144 at 3637.931 kb on - strand, within Pf6N2E2_3144 at 3637.931 kb on - strand, within Pf6N2E2_3144 at 3637.939 kb on - strand, within Pf6N2E2_3144 at 3637.999 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.122 kb on - strand, within Pf6N2E2_3144 at 3638.143 kb on - strand, within Pf6N2E2_3144 at 3638.176 kb on - strand, within Pf6N2E2_3144 at 3638.176 kb on - strand, within Pf6N2E2_3144 at 3638.430 kb on + strand at 3638.430 kb on + strand at 3638.430 kb on + strand at 3638.430 kb on + strand at 3638.430 kb on + strand at 3638.431 kb on - strand at 3638.431 kb on - strand at 3638.431 kb on - strand at 3638.431 kb on - strand at 3638.477 kb on + strand at 3638.477 kb on + strand at 3638.477 kb on + strand at 3638.477 kb on + strand at 3638.477 kb on + strand at 3638.477 kb on + strand at 3638.477 kb on + strand at 3638.477 kb on + strand at 3638.478 kb on - strand at 3638.478 kb on - strand at 3638.478 kb on - strand at 3638.478 kb on - strand at 3638.478 kb on - strand at 3638.645 kb on + strand at 3638.705 kb on + strand at 3638.705 kb on + strand at 3638.706 kb on - strand at 3639.284 kb on + strand, within Pf6N2E2_3145
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,637,517 - +0.9 3,637,879 - Pf6N2E2_3144 0.38 -0.8 3,637,879 - Pf6N2E2_3144 0.38 -0.4 3,637,879 - Pf6N2E2_3144 0.38 -1.5 3,637,931 - Pf6N2E2_3144 0.45 +0.1 3,637,931 - Pf6N2E2_3144 0.45 -2.4 3,637,939 - Pf6N2E2_3144 0.46 +0.4 3,637,999 - Pf6N2E2_3144 0.54 -1.1 3,638,122 - Pf6N2E2_3144 0.71 -1.8 3,638,122 - Pf6N2E2_3144 0.71 -2.6 3,638,122 - Pf6N2E2_3144 0.71 -0.3 3,638,122 - Pf6N2E2_3144 0.71 -1.3 3,638,122 - Pf6N2E2_3144 0.71 -0.2 3,638,122 - Pf6N2E2_3144 0.71 -1.7 3,638,122 - Pf6N2E2_3144 0.71 -0.5 3,638,122 - Pf6N2E2_3144 0.71 +0.2 3,638,122 - Pf6N2E2_3144 0.71 -1.2 3,638,122 - Pf6N2E2_3144 0.71 -1.6 3,638,143 - Pf6N2E2_3144 0.74 +0.2 3,638,176 - Pf6N2E2_3144 0.78 -0.2 3,638,176 - Pf6N2E2_3144 0.78 -1.9 3,638,430 + -0.3 3,638,430 + +0.0 3,638,430 + +1.0 3,638,430 + +0.5 3,638,430 + +0.7 3,638,431 - -0.5 3,638,431 - -0.9 3,638,431 - -0.7 3,638,431 - -0.0 3,638,477 + +0.4 3,638,477 + +0.2 3,638,477 + +0.5 3,638,477 + -0.0 3,638,477 + +0.3 3,638,477 + -0.8 3,638,477 + -1.6 3,638,477 + +0.2 3,638,478 - +0.8 3,638,478 - +0.3 3,638,478 - +0.6 3,638,478 - -1.2 3,638,478 - -1.2 3,638,645 + -0.5 3,638,705 + -2.6 3,638,705 + +1.2 3,638,706 - -0.2 3,639,284 + Pf6N2E2_3145 0.71 -3.7
Or see this region's nucleotide sequence