Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2973

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2972 and Pf6N2E2_2973 are separated by 17 nucleotidesPf6N2E2_2973 and Pf6N2E2_2974 are separated by 214 nucleotides Pf6N2E2_2972 - Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type, at 3,462,539 to 3,464,254 _2972 Pf6N2E2_2973 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,464,272 to 3,465,225 _2973 Pf6N2E2_2974 - DNA polymerase IV (EC 2.7.7.7), at 3,465,440 to 3,466,612 _2974 Position (kb) 3464 3465 3466Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3464.263 kb on - strandat 3464.291 kb on + strandat 3464.291 kb on + strandat 3464.291 kb on + strandat 3464.291 kb on + strandat 3464.292 kb on - strandat 3464.292 kb on - strandat 3464.292 kb on - strandat 3464.292 kb on - strandat 3464.515 kb on + strand, within Pf6N2E2_2973at 3464.525 kb on - strand, within Pf6N2E2_2973at 3464.555 kb on - strand, within Pf6N2E2_2973at 3464.665 kb on + strand, within Pf6N2E2_2973at 3464.665 kb on + strand, within Pf6N2E2_2973at 3464.665 kb on + strand, within Pf6N2E2_2973at 3464.665 kb on + strand, within Pf6N2E2_2973at 3464.666 kb on - strand, within Pf6N2E2_2973at 3464.666 kb on - strand, within Pf6N2E2_2973at 3464.666 kb on - strand, within Pf6N2E2_2973at 3464.666 kb on - strand, within Pf6N2E2_2973at 3465.023 kb on - strand, within Pf6N2E2_2973at 3465.023 kb on - strand, within Pf6N2E2_2973at 3465.314 kb on + strandat 3465.315 kb on - strandat 3465.315 kb on - strandat 3465.315 kb on - strandat 3465.316 kb on + strandat 3465.316 kb on + strandat 3465.316 kb on + strandat 3465.316 kb on + strandat 3465.317 kb on - strandat 3465.317 kb on - strandat 3465.317 kb on - strandat 3465.561 kb on + strand, within Pf6N2E2_2974at 3465.561 kb on + strand, within Pf6N2E2_2974at 3465.562 kb on - strand, within Pf6N2E2_2974at 3465.562 kb on - strand, within Pf6N2E2_2974at 3465.750 kb on + strand, within Pf6N2E2_2974at 3465.750 kb on + strand, within Pf6N2E2_2974at 3465.751 kb on - strand, within Pf6N2E2_2974at 3465.751 kb on - strand, within Pf6N2E2_2974at 3465.751 kb on - strand, within Pf6N2E2_2974at 3465.751 kb on - strand, within Pf6N2E2_2974at 3465.751 kb on - strand, within Pf6N2E2_2974at 3466.180 kb on - strand, within Pf6N2E2_2974at 3466.218 kb on + strand, within Pf6N2E2_2974at 3466.219 kb on - strand, within Pf6N2E2_2974at 3466.219 kb on - strand, within Pf6N2E2_2974

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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3,464,263 - -2.7
3,464,291 + -1.9
3,464,291 + -4.1
3,464,291 + -2.9
3,464,291 + -3.2
3,464,292 - -3.1
3,464,292 - -3.1
3,464,292 - -4.2
3,464,292 - -1.9
3,464,515 + Pf6N2E2_2973 0.25 -2.2
3,464,525 - Pf6N2E2_2973 0.27 -3.1
3,464,555 - Pf6N2E2_2973 0.30 -2.8
3,464,665 + Pf6N2E2_2973 0.41 -3.7
3,464,665 + Pf6N2E2_2973 0.41 -2.7
3,464,665 + Pf6N2E2_2973 0.41 -4.1
3,464,665 + Pf6N2E2_2973 0.41 +0.1
3,464,666 - Pf6N2E2_2973 0.41 -3.6
3,464,666 - Pf6N2E2_2973 0.41 -3.2
3,464,666 - Pf6N2E2_2973 0.41 -2.2
3,464,666 - Pf6N2E2_2973 0.41 -3.7
3,465,023 - Pf6N2E2_2973 0.79 -2.9
3,465,023 - Pf6N2E2_2973 0.79 -1.9
3,465,314 + +1.9
3,465,315 - -1.0
3,465,315 - -1.5
3,465,315 - -1.8
3,465,316 + -0.9
3,465,316 + -0.7
3,465,316 + +0.1
3,465,316 + -0.9
3,465,317 - -1.8
3,465,317 - -0.1
3,465,317 - -1.9
3,465,561 + Pf6N2E2_2974 0.10 -0.2
3,465,561 + Pf6N2E2_2974 0.10 -0.4
3,465,562 - Pf6N2E2_2974 0.10 +0.3
3,465,562 - Pf6N2E2_2974 0.10 +0.3
3,465,750 + Pf6N2E2_2974 0.26 -1.7
3,465,750 + Pf6N2E2_2974 0.26 -0.7
3,465,751 - Pf6N2E2_2974 0.27 +0.3
3,465,751 - Pf6N2E2_2974 0.27 +0.3
3,465,751 - Pf6N2E2_2974 0.27 -1.0
3,465,751 - Pf6N2E2_2974 0.27 +0.8
3,465,751 - Pf6N2E2_2974 0.27 +0.3
3,466,180 - Pf6N2E2_2974 0.63 +0.9
3,466,218 + Pf6N2E2_2974 0.66 +0.3
3,466,219 - Pf6N2E2_2974 0.66 +0.8
3,466,219 - Pf6N2E2_2974 0.66 -0.3

Or see this region's nucleotide sequence