Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2972 and Pf6N2E2_2973 are separated by 17 nucleotides Pf6N2E2_2973 and Pf6N2E2_2974 are separated by 214 nucleotides
Pf6N2E2_2972 - Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type, at 3,462,539 to 3,464,254
_2972
Pf6N2E2_2973 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,464,272 to 3,465,225
_2973
Pf6N2E2_2974 - DNA polymerase IV (EC 2.7.7.7), at 3,465,440 to 3,466,612
_2974
Position (kb)
3464
3465
3466 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3464.263 kb on - strand at 3464.291 kb on + strand at 3464.291 kb on + strand at 3464.291 kb on + strand at 3464.291 kb on + strand at 3464.292 kb on - strand at 3464.292 kb on - strand at 3464.292 kb on - strand at 3464.292 kb on - strand at 3464.515 kb on + strand, within Pf6N2E2_2973 at 3464.525 kb on - strand, within Pf6N2E2_2973 at 3464.555 kb on - strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3465.023 kb on - strand, within Pf6N2E2_2973 at 3465.023 kb on - strand, within Pf6N2E2_2973 at 3465.314 kb on + strand at 3465.315 kb on - strand at 3465.315 kb on - strand at 3465.315 kb on - strand at 3465.316 kb on + strand at 3465.316 kb on + strand at 3465.316 kb on + strand at 3465.316 kb on + strand at 3465.317 kb on - strand at 3465.317 kb on - strand at 3465.317 kb on - strand at 3465.561 kb on + strand, within Pf6N2E2_2974 at 3465.561 kb on + strand, within Pf6N2E2_2974 at 3465.562 kb on - strand, within Pf6N2E2_2974 at 3465.562 kb on - strand, within Pf6N2E2_2974 at 3465.750 kb on + strand, within Pf6N2E2_2974 at 3465.750 kb on + strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3466.180 kb on - strand, within Pf6N2E2_2974 at 3466.218 kb on + strand, within Pf6N2E2_2974 at 3466.219 kb on - strand, within Pf6N2E2_2974 at 3466.219 kb on - strand, within Pf6N2E2_2974
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,464,263 - -2.7 3,464,291 + -1.9 3,464,291 + -4.1 3,464,291 + -2.9 3,464,291 + -3.2 3,464,292 - -3.1 3,464,292 - -3.1 3,464,292 - -4.2 3,464,292 - -1.9 3,464,515 + Pf6N2E2_2973 0.25 -2.2 3,464,525 - Pf6N2E2_2973 0.27 -3.1 3,464,555 - Pf6N2E2_2973 0.30 -2.8 3,464,665 + Pf6N2E2_2973 0.41 -3.7 3,464,665 + Pf6N2E2_2973 0.41 -2.7 3,464,665 + Pf6N2E2_2973 0.41 -4.1 3,464,665 + Pf6N2E2_2973 0.41 +0.1 3,464,666 - Pf6N2E2_2973 0.41 -3.6 3,464,666 - Pf6N2E2_2973 0.41 -3.2 3,464,666 - Pf6N2E2_2973 0.41 -2.2 3,464,666 - Pf6N2E2_2973 0.41 -3.7 3,465,023 - Pf6N2E2_2973 0.79 -2.9 3,465,023 - Pf6N2E2_2973 0.79 -1.9 3,465,314 + +1.9 3,465,315 - -1.0 3,465,315 - -1.5 3,465,315 - -1.8 3,465,316 + -0.9 3,465,316 + -0.7 3,465,316 + +0.1 3,465,316 + -0.9 3,465,317 - -1.8 3,465,317 - -0.1 3,465,317 - -1.9 3,465,561 + Pf6N2E2_2974 0.10 -0.2 3,465,561 + Pf6N2E2_2974 0.10 -0.4 3,465,562 - Pf6N2E2_2974 0.10 +0.3 3,465,562 - Pf6N2E2_2974 0.10 +0.3 3,465,750 + Pf6N2E2_2974 0.26 -1.7 3,465,750 + Pf6N2E2_2974 0.26 -0.7 3,465,751 - Pf6N2E2_2974 0.27 +0.3 3,465,751 - Pf6N2E2_2974 0.27 +0.3 3,465,751 - Pf6N2E2_2974 0.27 -1.0 3,465,751 - Pf6N2E2_2974 0.27 +0.8 3,465,751 - Pf6N2E2_2974 0.27 +0.3 3,466,180 - Pf6N2E2_2974 0.63 +0.9 3,466,218 + Pf6N2E2_2974 0.66 +0.3 3,466,219 - Pf6N2E2_2974 0.66 +0.8 3,466,219 - Pf6N2E2_2974 0.66 -0.3
Or see this region's nucleotide sequence