Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2966 and Pf6N2E2_2967 are separated by 354 nucleotides Pf6N2E2_2967 and Pf6N2E2_2968 are separated by 31 nucleotides Pf6N2E2_2968 and Pf6N2E2_2969 are separated by 35 nucleotides
Pf6N2E2_2966 - Deoxycytidine triphosphate deaminase (EC 3.5.4.13), at 3,457,961 to 3,458,527
_2966
Pf6N2E2_2967 - Cold shock protein CspA, at 3,458,882 to 3,459,091
_2967
Pf6N2E2_2968 - hypothetical protein, at 3,459,123 to 3,459,251
_2968
Pf6N2E2_2969 - Peptide chain release factor RF-3, at 3,459,287 to 3,460,294
_2969
Position (kb)
3458
3459
3460 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3457.919 kb on + strand at 3457.919 kb on + strand at 3457.919 kb on + strand at 3457.919 kb on + strand at 3457.919 kb on + strand at 3457.920 kb on - strand at 3457.920 kb on - strand at 3457.920 kb on - strand at 3457.920 kb on - strand at 3457.920 kb on - strand at 3457.920 kb on - strand at 3457.920 kb on - strand at 3458.575 kb on - strand at 3458.575 kb on - strand at 3458.678 kb on - strand at 3458.725 kb on - strand at 3458.772 kb on + strand at 3458.772 kb on + strand at 3458.772 kb on + strand at 3458.772 kb on + strand at 3458.772 kb on + strand at 3458.773 kb on - strand at 3458.773 kb on - strand at 3458.773 kb on - strand at 3458.797 kb on + strand at 3458.858 kb on + strand at 3458.858 kb on + strand at 3458.908 kb on + strand, within Pf6N2E2_2967 at 3458.908 kb on + strand, within Pf6N2E2_2967 at 3458.908 kb on + strand, within Pf6N2E2_2967 at 3458.908 kb on + strand, within Pf6N2E2_2967 at 3459.085 kb on + strand at 3459.089 kb on + strand at 3459.089 kb on + strand at 3459.089 kb on + strand at 3459.108 kb on + strand at 3459.430 kb on - strand, within Pf6N2E2_2969 at 3459.435 kb on + strand, within Pf6N2E2_2969 at 3459.436 kb on - strand, within Pf6N2E2_2969 at 3459.436 kb on - strand, within Pf6N2E2_2969 at 3459.516 kb on + strand, within Pf6N2E2_2969 at 3459.517 kb on - strand, within Pf6N2E2_2969 at 3459.517 kb on - strand, within Pf6N2E2_2969 at 3459.517 kb on - strand, within Pf6N2E2_2969 at 3459.517 kb on - strand, within Pf6N2E2_2969 at 3459.517 kb on - strand, within Pf6N2E2_2969 at 3459.541 kb on - strand, within Pf6N2E2_2969 at 3459.541 kb on - strand, within Pf6N2E2_2969 at 3459.541 kb on - strand, within Pf6N2E2_2969 at 3459.568 kb on - strand, within Pf6N2E2_2969 at 3459.573 kb on + strand, within Pf6N2E2_2969 at 3459.574 kb on - strand, within Pf6N2E2_2969 at 3459.696 kb on + strand, within Pf6N2E2_2969 at 3459.696 kb on + strand, within Pf6N2E2_2969 at 3459.697 kb on - strand, within Pf6N2E2_2969 at 3459.697 kb on - strand, within Pf6N2E2_2969 at 3459.804 kb on + strand, within Pf6N2E2_2969 at 3459.804 kb on + strand, within Pf6N2E2_2969 at 3459.804 kb on + strand, within Pf6N2E2_2969 at 3459.804 kb on + strand, within Pf6N2E2_2969 at 3459.804 kb on + strand, within Pf6N2E2_2969 at 3459.804 kb on + strand, within Pf6N2E2_2969 at 3459.805 kb on - strand, within Pf6N2E2_2969 at 3459.805 kb on - strand, within Pf6N2E2_2969 at 3459.821 kb on - strand, within Pf6N2E2_2969 at 3460.068 kb on + strand, within Pf6N2E2_2969 at 3460.068 kb on + strand, within Pf6N2E2_2969 at 3460.068 kb on + strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969 at 3460.069 kb on - strand, within Pf6N2E2_2969
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,457,919 + +0.1 3,457,919 + -0.5 3,457,919 + -0.3 3,457,919 + -1.3 3,457,919 + -2.6 3,457,920 - -1.1 3,457,920 - +0.2 3,457,920 - -0.9 3,457,920 - -0.1 3,457,920 - -0.0 3,457,920 - -0.3 3,457,920 - -0.6 3,458,575 - -1.1 3,458,575 - -2.6 3,458,678 - -2.0 3,458,725 - -3.2 3,458,772 + -3.3 3,458,772 + -1.9 3,458,772 + -3.2 3,458,772 + -2.3 3,458,772 + -3.1 3,458,773 - -0.3 3,458,773 - -1.2 3,458,773 - -0.8 3,458,797 + -3.0 3,458,858 + -2.1 3,458,858 + -2.3 3,458,908 + Pf6N2E2_2967 0.12 -3.7 3,458,908 + Pf6N2E2_2967 0.12 -0.9 3,458,908 + Pf6N2E2_2967 0.12 -3.1 3,458,908 + Pf6N2E2_2967 0.12 -1.2 3,459,085 + -3.4 3,459,089 + -3.5 3,459,089 + -3.9 3,459,089 + -3.5 3,459,108 + -3.5 3,459,430 - Pf6N2E2_2969 0.14 -0.2 3,459,435 + Pf6N2E2_2969 0.15 -3.3 3,459,436 - Pf6N2E2_2969 0.15 -1.9 3,459,436 - Pf6N2E2_2969 0.15 +0.1 3,459,516 + Pf6N2E2_2969 0.23 -0.5 3,459,517 - Pf6N2E2_2969 0.23 -0.3 3,459,517 - Pf6N2E2_2969 0.23 -1.3 3,459,517 - Pf6N2E2_2969 0.23 -0.2 3,459,517 - Pf6N2E2_2969 0.23 +0.1 3,459,517 - Pf6N2E2_2969 0.23 +0.0 3,459,541 - Pf6N2E2_2969 0.25 -0.0 3,459,541 - Pf6N2E2_2969 0.25 -1.0 3,459,541 - Pf6N2E2_2969 0.25 -0.2 3,459,568 - Pf6N2E2_2969 0.28 -1.2 3,459,573 + Pf6N2E2_2969 0.28 -2.1 3,459,574 - Pf6N2E2_2969 0.28 -0.8 3,459,696 + Pf6N2E2_2969 0.41 -2.8 3,459,696 + Pf6N2E2_2969 0.41 -1.1 3,459,697 - Pf6N2E2_2969 0.41 -0.4 3,459,697 - Pf6N2E2_2969 0.41 -0.6 3,459,804 + Pf6N2E2_2969 0.51 -1.9 3,459,804 + Pf6N2E2_2969 0.51 -1.2 3,459,804 + Pf6N2E2_2969 0.51 +0.2 3,459,804 + Pf6N2E2_2969 0.51 -0.4 3,459,804 + Pf6N2E2_2969 0.51 -1.2 3,459,804 + Pf6N2E2_2969 0.51 +0.5 3,459,805 - Pf6N2E2_2969 0.51 -0.6 3,459,805 - Pf6N2E2_2969 0.51 +0.1 3,459,821 - Pf6N2E2_2969 0.53 -0.5 3,460,068 + Pf6N2E2_2969 0.77 -1.5 3,460,068 + Pf6N2E2_2969 0.77 -1.2 3,460,068 + Pf6N2E2_2969 0.77 -1.8 3,460,069 - Pf6N2E2_2969 0.78 -0.2 3,460,069 - Pf6N2E2_2969 0.78 -1.2 3,460,069 - Pf6N2E2_2969 0.78 +0.0 3,460,069 - Pf6N2E2_2969 0.78 -0.9 3,460,069 - Pf6N2E2_2969 0.78 +1.0 3,460,069 - Pf6N2E2_2969 0.78 -0.2 3,460,069 - Pf6N2E2_2969 0.78 +0.1 3,460,069 - Pf6N2E2_2969 0.78 -0.7 3,460,069 - Pf6N2E2_2969 0.78 -1.5 3,460,069 - Pf6N2E2_2969 0.78 +1.1
Or see this region's nucleotide sequence