Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2898 and Pf6N2E2_2899 are separated by 218 nucleotides Pf6N2E2_2899 and Pf6N2E2_2900 are separated by 247 nucleotides Pf6N2E2_2900 and Pf6N2E2_2901 are separated by 156 nucleotides
Pf6N2E2_2898 - Phosphogluconate dehydratase (EC 4.2.1.12), at 3,386,886 to 3,388,712
_2898
Pf6N2E2_2899 - NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), at 3,388,931 to 3,389,932
_2899
Pf6N2E2_2900 - FIG006045: Sigma factor, ECF subfamily, at 3,390,180 to 3,390,701
_2900
Pf6N2E2_2901 - Iron siderophore sensor protein, at 3,390,858 to 3,391,763
_2901
Position (kb)
3388
3389
3390 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3388.755 kb on - strand at 3388.755 kb on - strand at 3388.755 kb on - strand at 3388.889 kb on + strand at 3388.994 kb on + strand at 3388.994 kb on + strand at 3388.995 kb on - strand at 3388.995 kb on - strand at 3388.995 kb on - strand at 3388.995 kb on - strand at 3388.995 kb on - strand at 3389.266 kb on + strand, within Pf6N2E2_2899 at 3389.267 kb on - strand, within Pf6N2E2_2899 at 3389.267 kb on - strand, within Pf6N2E2_2899 at 3389.333 kb on + strand, within Pf6N2E2_2899 at 3389.477 kb on + strand, within Pf6N2E2_2899 at 3389.478 kb on - strand, within Pf6N2E2_2899 at 3389.478 kb on - strand, within Pf6N2E2_2899 at 3389.478 kb on - strand, within Pf6N2E2_2899 at 3389.478 kb on - strand, within Pf6N2E2_2899 at 3389.507 kb on + strand, within Pf6N2E2_2899 at 3389.508 kb on - strand, within Pf6N2E2_2899 at 3389.864 kb on + strand at 3389.864 kb on + strand at 3389.864 kb on + strand at 3389.864 kb on + strand at 3389.865 kb on - strand at 3389.902 kb on + strand at 3389.930 kb on + strand at 3389.930 kb on + strand at 3389.931 kb on - strand at 3389.931 kb on - strand at 3389.963 kb on + strand at 3389.963 kb on + strand at 3389.964 kb on - strand at 3389.964 kb on - strand at 3390.294 kb on + strand, within Pf6N2E2_2900 at 3390.295 kb on - strand, within Pf6N2E2_2900 at 3390.431 kb on - strand, within Pf6N2E2_2900 at 3390.588 kb on + strand, within Pf6N2E2_2900 at 3390.589 kb on - strand, within Pf6N2E2_2900 at 3390.589 kb on - strand, within Pf6N2E2_2900 at 3390.683 kb on + strand at 3390.724 kb on + strand at 3390.724 kb on + strand at 3390.725 kb on - strand at 3390.725 kb on - strand at 3390.725 kb on - strand at 3390.725 kb on - strand at 3390.734 kb on - strand at 3390.755 kb on + strand at 3390.755 kb on + strand at 3390.756 kb on - strand at 3390.756 kb on - strand at 3390.764 kb on + strand at 3390.765 kb on - strand at 3390.765 kb on - strand at 3390.783 kb on + strand at 3390.784 kb on - strand at 3390.787 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,388,755 - +0.3 3,388,755 - -0.2 3,388,755 - +0.9 3,388,889 + +2.0 3,388,994 + +0.1 3,388,994 + +1.6 3,388,995 - +0.9 3,388,995 - +0.1 3,388,995 - -0.2 3,388,995 - -0.6 3,388,995 - +0.9 3,389,266 + Pf6N2E2_2899 0.33 -2.1 3,389,267 - Pf6N2E2_2899 0.34 -2.5 3,389,267 - Pf6N2E2_2899 0.34 -1.3 3,389,333 + Pf6N2E2_2899 0.40 -1.0 3,389,477 + Pf6N2E2_2899 0.54 -1.9 3,389,478 - Pf6N2E2_2899 0.55 +0.5 3,389,478 - Pf6N2E2_2899 0.55 -1.1 3,389,478 - Pf6N2E2_2899 0.55 -1.2 3,389,478 - Pf6N2E2_2899 0.55 -1.3 3,389,507 + Pf6N2E2_2899 0.57 -1.9 3,389,508 - Pf6N2E2_2899 0.58 -0.7 3,389,864 + -0.9 3,389,864 + -1.4 3,389,864 + -1.0 3,389,864 + -1.2 3,389,865 - -1.8 3,389,902 + -1.5 3,389,930 + -0.3 3,389,930 + +0.4 3,389,931 - -0.3 3,389,931 - -0.9 3,389,963 + -1.4 3,389,963 + +1.1 3,389,964 - -0.3 3,389,964 - +1.9 3,390,294 + Pf6N2E2_2900 0.22 +0.3 3,390,295 - Pf6N2E2_2900 0.22 -0.2 3,390,431 - Pf6N2E2_2900 0.48 +0.9 3,390,588 + Pf6N2E2_2900 0.78 -1.7 3,390,589 - Pf6N2E2_2900 0.78 +0.7 3,390,589 - Pf6N2E2_2900 0.78 +0.8 3,390,683 + +0.3 3,390,724 + -1.5 3,390,724 + +0.1 3,390,725 - -0.1 3,390,725 - -1.1 3,390,725 - +1.2 3,390,725 - +0.4 3,390,734 - +1.1 3,390,755 + +0.6 3,390,755 + +0.9 3,390,756 - +0.5 3,390,756 - -0.3 3,390,764 + +0.4 3,390,765 - +0.2 3,390,765 - +0.9 3,390,783 + -0.3 3,390,784 - +0.6 3,390,787 - -0.3
Or see this region's nucleotide sequence