Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2882 and Pf6N2E2_2883 are separated by 233 nucleotides Pf6N2E2_2883 and Pf6N2E2_2884 are separated by 17 nucleotides Pf6N2E2_2884 and Pf6N2E2_2885 overlap by 14 nucleotides
Pf6N2E2_2882 - Isopenicillin N epimerase (EC 5.1.1.17), at 3,369,514 to 3,370,701
_2882
Pf6N2E2_2883 - 4-Hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14), at 3,370,935 to 3,371,600
_2883
Pf6N2E2_2884 - 6-phosphogluconolactonase (EC 3.1.1.31), eukaryotic type, at 3,371,618 to 3,372,331
_2884
Pf6N2E2_2885 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 3,372,318 to 3,373,787
_2885
Position (kb)
3371
3372
3373 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.852 kb on + strand at 3370.852 kb on + strand at 3370.853 kb on - strand at 3370.853 kb on - strand at 3370.898 kb on - strand at 3370.905 kb on + strand at 3370.906 kb on - strand at 3371.632 kb on - strand at 3371.632 kb on - strand at 3371.632 kb on - strand at 3371.645 kb on - strand at 3371.645 kb on - strand at 3372.332 kb on - strand at 3372.533 kb on - strand, within Pf6N2E2_2885 at 3372.560 kb on - strand, within Pf6N2E2_2885 at 3372.722 kb on - strand, within Pf6N2E2_2885 at 3372.722 kb on - strand, within Pf6N2E2_2885 at 3372.932 kb on - strand, within Pf6N2E2_2885 at 3372.932 kb on - strand, within Pf6N2E2_2885 at 3372.932 kb on - strand, within Pf6N2E2_2885 at 3372.932 kb on - strand, within Pf6N2E2_2885 at 3373.181 kb on - strand, within Pf6N2E2_2885 at 3373.262 kb on - strand, within Pf6N2E2_2885 at 3373.277 kb on - strand, within Pf6N2E2_2885 at 3373.277 kb on - strand, within Pf6N2E2_2885 at 3373.281 kb on - strand, within Pf6N2E2_2885 at 3373.281 kb on - strand, within Pf6N2E2_2885
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,370,699 + -0.5 3,370,699 + +0.5 3,370,699 + +1.3 3,370,699 + -1.7 3,370,699 + -0.1 3,370,699 + +1.7 3,370,699 + -0.6 3,370,699 + +1.3 3,370,699 + -0.6 3,370,699 + +1.1 3,370,700 - +0.2 3,370,700 - +0.2 3,370,700 - +0.2 3,370,700 - +1.3 3,370,700 - -0.4 3,370,700 - +0.2 3,370,700 - +0.6 3,370,700 - +1.1 3,370,700 - +1.6 3,370,700 - +0.2 3,370,700 - +1.7 3,370,700 - +1.8 3,370,700 - +0.7 3,370,700 - +0.1 3,370,852 + +0.4 3,370,852 + -0.4 3,370,853 - -0.8 3,370,853 - -0.5 3,370,898 - +1.8 3,370,905 + +0.8 3,370,906 - -0.3 3,371,632 - +3.7 3,371,632 - +3.3 3,371,632 - +6.3 3,371,645 - +1.3 3,371,645 - +5.5 3,372,332 - +1.7 3,372,533 - Pf6N2E2_2885 0.15 -0.8 3,372,560 - Pf6N2E2_2885 0.16 -2.5 3,372,722 - Pf6N2E2_2885 0.27 -0.1 3,372,722 - Pf6N2E2_2885 0.27 -3.4 3,372,932 - Pf6N2E2_2885 0.42 -0.3 3,372,932 - Pf6N2E2_2885 0.42 -1.5 3,372,932 - Pf6N2E2_2885 0.42 +1.6 3,372,932 - Pf6N2E2_2885 0.42 -2.1 3,373,181 - Pf6N2E2_2885 0.59 +0.1 3,373,262 - Pf6N2E2_2885 0.64 +1.6 3,373,277 - Pf6N2E2_2885 0.65 +1.7 3,373,277 - Pf6N2E2_2885 0.65 +1.5 3,373,281 - Pf6N2E2_2885 0.66 +1.2 3,373,281 - Pf6N2E2_2885 0.66 -0.5
Or see this region's nucleotide sequence