Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2744 and Pf6N2E2_2745 are separated by 27 nucleotides Pf6N2E2_2745 and Pf6N2E2_2746 are separated by 4 nucleotides Pf6N2E2_2746 and Pf6N2E2_2747 are separated by 17 nucleotides
Pf6N2E2_2744 - Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6), at 3,237,050 to 3,237,763
_2744
Pf6N2E2_2745 - Metal-dependent hydrolases of the beta-lactamase superfamily I, at 3,237,791 to 3,238,549
_2745
Pf6N2E2_2746 - Outer membrane protein NlpB, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and YfgL); Lipoprotein-34 precursor, at 3,238,554 to 3,239,669
_2746
Pf6N2E2_2747 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 3,239,687 to 3,240,565
_2747
Position (kb)
3238
3239
3240 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3237.781 kb on - strand at 3237.781 kb on - strand at 3237.781 kb on - strand at 3237.781 kb on - strand at 3237.781 kb on - strand at 3237.781 kb on - strand at 3237.785 kb on - strand at 3237.800 kb on - strand at 3238.030 kb on - strand, within Pf6N2E2_2745 at 3238.030 kb on - strand, within Pf6N2E2_2745 at 3238.030 kb on - strand, within Pf6N2E2_2745 at 3238.039 kb on - strand, within Pf6N2E2_2745 at 3238.039 kb on - strand, within Pf6N2E2_2745 at 3238.102 kb on - strand, within Pf6N2E2_2745 at 3238.102 kb on - strand, within Pf6N2E2_2745 at 3238.180 kb on - strand, within Pf6N2E2_2745 at 3238.180 kb on - strand, within Pf6N2E2_2745 at 3238.180 kb on - strand, within Pf6N2E2_2745 at 3238.180 kb on - strand, within Pf6N2E2_2745 at 3238.180 kb on - strand, within Pf6N2E2_2745 at 3238.180 kb on - strand, within Pf6N2E2_2745 at 3238.180 kb on - strand, within Pf6N2E2_2745 at 3238.180 kb on - strand, within Pf6N2E2_2745 at 3238.180 kb on - strand, within Pf6N2E2_2745 at 3238.180 kb on - strand, within Pf6N2E2_2745 at 3238.180 kb on - strand, within Pf6N2E2_2745 at 3238.483 kb on - strand at 3238.483 kb on - strand at 3238.483 kb on - strand at 3238.483 kb on - strand at 3238.483 kb on - strand at 3238.483 kb on - strand at 3238.483 kb on - strand at 3238.483 kb on - strand at 3238.483 kb on - strand at 3238.483 kb on - strand at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3239.114 kb on - strand, within Pf6N2E2_2746 at 3239.114 kb on - strand, within Pf6N2E2_2746 at 3239.114 kb on - strand, within Pf6N2E2_2746 at 3239.114 kb on - strand, within Pf6N2E2_2746 at 3239.390 kb on - strand, within Pf6N2E2_2746 at 3239.441 kb on - strand, within Pf6N2E2_2746 at 3239.441 kb on - strand, within Pf6N2E2_2746 at 3239.585 kb on - strand at 3239.631 kb on - strand at 3239.631 kb on - strand at 3239.689 kb on - strand at 3240.633 kb on + strand at 3240.633 kb on + strand at 3240.634 kb on - strand at 3240.634 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,237,781 - -1.4 3,237,781 - +1.2 3,237,781 - +0.4 3,237,781 - -0.1 3,237,781 - +0.1 3,237,781 - -0.6 3,237,785 - +0.9 3,237,800 - +0.7 3,238,030 - Pf6N2E2_2745 0.31 +1.2 3,238,030 - Pf6N2E2_2745 0.31 -0.8 3,238,030 - Pf6N2E2_2745 0.31 +1.4 3,238,039 - Pf6N2E2_2745 0.33 -0.4 3,238,039 - Pf6N2E2_2745 0.33 -0.5 3,238,102 - Pf6N2E2_2745 0.41 -0.7 3,238,102 - Pf6N2E2_2745 0.41 +0.5 3,238,180 - Pf6N2E2_2745 0.51 -0.2 3,238,180 - Pf6N2E2_2745 0.51 -1.8 3,238,180 - Pf6N2E2_2745 0.51 -0.4 3,238,180 - Pf6N2E2_2745 0.51 -1.6 3,238,180 - Pf6N2E2_2745 0.51 +0.1 3,238,180 - Pf6N2E2_2745 0.51 -1.1 3,238,180 - Pf6N2E2_2745 0.51 +0.5 3,238,180 - Pf6N2E2_2745 0.51 -0.4 3,238,180 - Pf6N2E2_2745 0.51 -0.3 3,238,180 - Pf6N2E2_2745 0.51 -1.1 3,238,180 - Pf6N2E2_2745 0.51 +0.7 3,238,483 - +0.6 3,238,483 - -0.5 3,238,483 - +1.1 3,238,483 - +0.1 3,238,483 - -0.3 3,238,483 - -2.4 3,238,483 - +1.0 3,238,483 - +1.0 3,238,483 - +1.7 3,238,483 - +0.0 3,238,790 - Pf6N2E2_2746 0.21 -1.3 3,238,790 - Pf6N2E2_2746 0.21 -1.1 3,238,790 - Pf6N2E2_2746 0.21 -1.5 3,238,790 - Pf6N2E2_2746 0.21 -2.9 3,238,790 - Pf6N2E2_2746 0.21 -1.5 3,238,790 - Pf6N2E2_2746 0.21 -2.6 3,238,793 - Pf6N2E2_2746 0.21 -0.6 3,238,793 - Pf6N2E2_2746 0.21 +1.3 3,238,793 - Pf6N2E2_2746 0.21 +0.2 3,238,793 - Pf6N2E2_2746 0.21 -0.4 3,238,793 - Pf6N2E2_2746 0.21 -1.0 3,238,793 - Pf6N2E2_2746 0.21 +0.2 3,238,793 - Pf6N2E2_2746 0.21 -1.7 3,239,114 - Pf6N2E2_2746 0.50 +0.4 3,239,114 - Pf6N2E2_2746 0.50 +0.4 3,239,114 - Pf6N2E2_2746 0.50 -1.6 3,239,114 - Pf6N2E2_2746 0.50 -0.8 3,239,390 - Pf6N2E2_2746 0.75 -0.4 3,239,441 - Pf6N2E2_2746 0.79 -2.6 3,239,441 - Pf6N2E2_2746 0.79 -1.8 3,239,585 - -0.9 3,239,631 - -1.0 3,239,631 - -0.0 3,239,689 - +0.9 3,240,633 + -1.8 3,240,633 + +0.6 3,240,634 - -0.4 3,240,634 - -1.1
Or see this region's nucleotide sequence