Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2698 and Pf6N2E2_2699 are separated by 338 nucleotides Pf6N2E2_2699 and Pf6N2E2_2700 are separated by 431 nucleotides
Pf6N2E2_2698 - Permease of the drug/metabolite transporter (DMT) superfamily, at 3,191,394 to 3,192,257
_2698
Pf6N2E2_2699 - Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO, at 3,192,596 to 3,193,534
_2699
Pf6N2E2_2700 - Cysteine synthase (EC 2.5.1.47), at 3,193,966 to 3,194,940
_2700
Position (kb)
3192
3193
3194 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3191.642 kb on - strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.837 kb on - strand, within Pf6N2E2_2698 at 3191.837 kb on - strand, within Pf6N2E2_2698 at 3192.261 kb on + strand at 3192.270 kb on + strand at 3192.270 kb on + strand at 3192.270 kb on + strand at 3192.271 kb on - strand at 3192.271 kb on - strand at 3192.271 kb on - strand at 3192.271 kb on - strand at 3192.384 kb on - strand at 3192.384 kb on - strand at 3192.393 kb on + strand at 3192.394 kb on - strand at 3192.394 kb on - strand at 3192.406 kb on + strand at 3192.423 kb on + strand at 3192.566 kb on - strand at 3192.586 kb on + strand at 3192.593 kb on + strand at 3192.594 kb on - strand at 3192.598 kb on - strand at 3192.738 kb on + strand, within Pf6N2E2_2699 at 3192.738 kb on + strand, within Pf6N2E2_2699 at 3192.918 kb on + strand, within Pf6N2E2_2699 at 3192.919 kb on - strand, within Pf6N2E2_2699 at 3192.919 kb on - strand, within Pf6N2E2_2699 at 3192.919 kb on - strand, within Pf6N2E2_2699 at 3192.919 kb on - strand, within Pf6N2E2_2699 at 3192.961 kb on - strand, within Pf6N2E2_2699 at 3192.961 kb on - strand, within Pf6N2E2_2699 at 3192.961 kb on - strand, within Pf6N2E2_2699 at 3193.189 kb on - strand, within Pf6N2E2_2699 at 3193.261 kb on - strand, within Pf6N2E2_2699 at 3193.261 kb on - strand, within Pf6N2E2_2699 at 3193.261 kb on - strand, within Pf6N2E2_2699 at 3193.353 kb on + strand, within Pf6N2E2_2699 at 3193.353 kb on + strand, within Pf6N2E2_2699 at 3193.353 kb on + strand, within Pf6N2E2_2699 at 3193.383 kb on + strand, within Pf6N2E2_2699 at 3193.384 kb on - strand, within Pf6N2E2_2699 at 3193.396 kb on + strand, within Pf6N2E2_2699 at 3193.397 kb on - strand, within Pf6N2E2_2699 at 3193.399 kb on - strand, within Pf6N2E2_2699 at 3193.399 kb on - strand, within Pf6N2E2_2699 at 3193.399 kb on - strand, within Pf6N2E2_2699 at 3193.473 kb on + strand at 3193.473 kb on + strand at 3193.575 kb on - strand at 3193.586 kb on + strand at 3193.675 kb on + strand at 3193.675 kb on + strand at 3193.725 kb on + strand at 3193.725 kb on + strand at 3193.726 kb on - strand at 3193.726 kb on - strand at 3193.726 kb on - strand at 3193.726 kb on - strand at 3193.889 kb on + strand at 3193.889 kb on + strand at 3193.890 kb on - strand at 3193.890 kb on - strand at 3194.021 kb on + strand at 3194.021 kb on + strand at 3194.022 kb on - strand at 3194.189 kb on - strand, within Pf6N2E2_2700 at 3194.373 kb on - strand, within Pf6N2E2_2700 at 3194.373 kb on - strand, within Pf6N2E2_2700 at 3194.373 kb on - strand, within Pf6N2E2_2700 at 3194.373 kb on - strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.523 kb on - strand, within Pf6N2E2_2700 at 3194.523 kb on - strand, within Pf6N2E2_2700
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,191,642 - Pf6N2E2_2698 0.29 +0.6 3,191,836 + Pf6N2E2_2698 0.51 -0.3 3,191,836 + Pf6N2E2_2698 0.51 -0.7 3,191,836 + Pf6N2E2_2698 0.51 -0.4 3,191,836 + Pf6N2E2_2698 0.51 -1.4 3,191,836 + Pf6N2E2_2698 0.51 +2.5 3,191,837 - Pf6N2E2_2698 0.51 +0.1 3,191,837 - Pf6N2E2_2698 0.51 +0.8 3,192,261 + -1.1 3,192,270 + +0.9 3,192,270 + +0.9 3,192,270 + -0.5 3,192,271 - -0.8 3,192,271 - -1.7 3,192,271 - -0.7 3,192,271 - -1.4 3,192,384 - +2.4 3,192,384 - -0.8 3,192,393 + +0.3 3,192,394 - +0.4 3,192,394 - -0.9 3,192,406 + +0.1 3,192,423 + +0.3 3,192,566 - -0.3 3,192,586 + -0.3 3,192,593 + -3.1 3,192,594 - +0.5 3,192,598 - +0.8 3,192,738 + Pf6N2E2_2699 0.15 -1.1 3,192,738 + Pf6N2E2_2699 0.15 -2.7 3,192,918 + Pf6N2E2_2699 0.34 -1.1 3,192,919 - Pf6N2E2_2699 0.34 -1.9 3,192,919 - Pf6N2E2_2699 0.34 -1.1 3,192,919 - Pf6N2E2_2699 0.34 -4.4 3,192,919 - Pf6N2E2_2699 0.34 -3.1 3,192,961 - Pf6N2E2_2699 0.39 -4.0 3,192,961 - Pf6N2E2_2699 0.39 -3.1 3,192,961 - Pf6N2E2_2699 0.39 -0.1 3,193,189 - Pf6N2E2_2699 0.63 -2.5 3,193,261 - Pf6N2E2_2699 0.71 -1.0 3,193,261 - Pf6N2E2_2699 0.71 -4.2 3,193,261 - Pf6N2E2_2699 0.71 -1.8 3,193,353 + Pf6N2E2_2699 0.81 -0.9 3,193,353 + Pf6N2E2_2699 0.81 -4.3 3,193,353 + Pf6N2E2_2699 0.81 -2.1 3,193,383 + Pf6N2E2_2699 0.84 -1.9 3,193,384 - Pf6N2E2_2699 0.84 -2.6 3,193,396 + Pf6N2E2_2699 0.85 -4.3 3,193,397 - Pf6N2E2_2699 0.85 -1.4 3,193,399 - Pf6N2E2_2699 0.86 -0.5 3,193,399 - Pf6N2E2_2699 0.86 -1.7 3,193,399 - Pf6N2E2_2699 0.86 -1.7 3,193,473 + -3.5 3,193,473 + -1.4 3,193,575 - +0.2 3,193,586 + -2.1 3,193,675 + -2.7 3,193,675 + -1.5 3,193,725 + -1.5 3,193,725 + -0.8 3,193,726 - +0.6 3,193,726 - -0.2 3,193,726 - +2.4 3,193,726 - +0.1 3,193,889 + -0.2 3,193,889 + -1.1 3,193,890 - +0.6 3,193,890 - +0.4 3,194,021 + +0.2 3,194,021 + +0.9 3,194,022 - -1.3 3,194,189 - Pf6N2E2_2700 0.23 +0.6 3,194,373 - Pf6N2E2_2700 0.42 +0.4 3,194,373 - Pf6N2E2_2700 0.42 -0.7 3,194,373 - Pf6N2E2_2700 0.42 -0.4 3,194,373 - Pf6N2E2_2700 0.42 -0.3 3,194,522 + Pf6N2E2_2700 0.57 +0.5 3,194,522 + Pf6N2E2_2700 0.57 +0.2 3,194,522 + Pf6N2E2_2700 0.57 -0.3 3,194,522 + Pf6N2E2_2700 0.57 +1.9 3,194,522 + Pf6N2E2_2700 0.57 +0.1 3,194,523 - Pf6N2E2_2700 0.57 -2.3 3,194,523 - Pf6N2E2_2700 0.57 -0.3
Or see this region's nucleotide sequence