Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2693 and Pf6N2E2_2694 are separated by 248 nucleotides Pf6N2E2_2694 and Pf6N2E2_2695 are separated by 24 nucleotides
Pf6N2E2_2693 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180), at 3,182,153 to 3,183,274
_2693
Pf6N2E2_2694 - ATP-dependent RNA helicase HrpA (EC 3.6.4.13), at 3,183,523 to 3,187,434
_2694
Pf6N2E2_2695 - hypothetical protein, at 3,187,459 to 3,187,584
_2695
Position (kb)
3183
3184
3185
3186
3187
3188 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3182.881 kb on - strand, within Pf6N2E2_2693 at 3182.881 kb on - strand, within Pf6N2E2_2693 at 3183.186 kb on + strand at 3183.186 kb on + strand at 3183.186 kb on + strand at 3183.186 kb on + strand at 3183.186 kb on + strand at 3183.187 kb on - strand at 3183.187 kb on - strand at 3183.187 kb on - strand at 3183.187 kb on - strand at 3183.187 kb on - strand at 3183.187 kb on - strand at 3183.187 kb on - strand at 3183.187 kb on - strand at 3183.187 kb on - strand at 3183.187 kb on - strand at 3183.187 kb on - strand at 3183.187 kb on - strand at 3183.191 kb on - strand at 3183.191 kb on - strand at 3183.191 kb on - strand at 3183.240 kb on + strand at 3183.240 kb on + strand at 3183.240 kb on + strand at 3183.241 kb on - strand at 3183.241 kb on - strand at 3183.241 kb on - strand at 3183.241 kb on - strand at 3183.241 kb on - strand at 3183.241 kb on - strand at 3183.269 kb on - strand at 3183.320 kb on + strand at 3183.320 kb on + strand at 3183.321 kb on - strand at 3183.321 kb on - strand at 3183.321 kb on - strand at 3183.321 kb on - strand at 3183.321 kb on - strand at 3183.326 kb on + strand at 3183.327 kb on - strand at 3183.424 kb on + strand at 3183.424 kb on + strand at 3183.424 kb on + strand at 3183.425 kb on - strand at 3183.425 kb on - strand at 3183.425 kb on - strand at 3183.425 kb on - strand at 3183.427 kb on - strand at 3183.477 kb on + strand at 3183.477 kb on + strand at 3183.477 kb on + strand at 3183.477 kb on + strand at 3183.478 kb on - strand at 3183.478 kb on - strand at 3183.478 kb on - strand at 3183.478 kb on - strand at 3183.478 kb on - strand at 3183.478 kb on - strand at 3183.478 kb on - strand at 3183.480 kb on + strand at 3183.480 kb on + strand at 3183.480 kb on + strand at 3183.481 kb on - strand at 3183.481 kb on - strand at 3183.481 kb on - strand at 3183.481 kb on - strand at 3183.484 kb on + strand at 3183.845 kb on + strand at 3183.846 kb on - strand at 3183.874 kb on - strand at 3184.133 kb on + strand, within Pf6N2E2_2694 at 3184.308 kb on - strand, within Pf6N2E2_2694 at 3184.844 kb on + strand, within Pf6N2E2_2694 at 3184.845 kb on - strand, within Pf6N2E2_2694 at 3184.845 kb on - strand, within Pf6N2E2_2694 at 3184.845 kb on - strand, within Pf6N2E2_2694 at 3184.845 kb on - strand, within Pf6N2E2_2694 at 3185.036 kb on + strand, within Pf6N2E2_2694 at 3185.037 kb on - strand, within Pf6N2E2_2694 at 3185.045 kb on + strand, within Pf6N2E2_2694 at 3185.045 kb on + strand, within Pf6N2E2_2694 at 3185.046 kb on - strand, within Pf6N2E2_2694 at 3185.046 kb on - strand, within Pf6N2E2_2694 at 3185.276 kb on + strand, within Pf6N2E2_2694 at 3185.276 kb on + strand, within Pf6N2E2_2694 at 3185.277 kb on - strand, within Pf6N2E2_2694 at 3185.277 kb on - strand, within Pf6N2E2_2694 at 3185.393 kb on + strand, within Pf6N2E2_2694 at 3185.394 kb on - strand, within Pf6N2E2_2694 at 3185.394 kb on - strand, within Pf6N2E2_2694 at 3185.667 kb on - strand, within Pf6N2E2_2694 at 3185.667 kb on - strand, within Pf6N2E2_2694 at 3185.667 kb on - strand, within Pf6N2E2_2694 at 3185.667 kb on - strand, within Pf6N2E2_2694 at 3185.667 kb on - strand, within Pf6N2E2_2694 at 3186.179 kb on + strand, within Pf6N2E2_2694 at 3186.180 kb on - strand, within Pf6N2E2_2694 at 3186.288 kb on - strand, within Pf6N2E2_2694 at 3186.444 kb on - strand, within Pf6N2E2_2694 at 3186.444 kb on - strand, within Pf6N2E2_2694 at 3186.444 kb on - strand, within Pf6N2E2_2694 at 3186.480 kb on + strand, within Pf6N2E2_2694 at 3186.570 kb on - strand, within Pf6N2E2_2694 at 3186.878 kb on + strand, within Pf6N2E2_2694 at 3187.439 kb on + strand at 3187.439 kb on + strand at 3187.440 kb on - strand at 3187.575 kb on + strand at 3187.575 kb on + strand at 3187.575 kb on + strand at 3187.576 kb on - strand at 3187.576 kb on - strand at 3187.576 kb on - strand at 3187.576 kb on - strand at 3187.591 kb on + strand at 3187.591 kb on + strand at 3187.591 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,182,881 - Pf6N2E2_2693 0.65 -0.6 3,182,881 - Pf6N2E2_2693 0.65 -0.1 3,183,186 + -2.4 3,183,186 + +0.3 3,183,186 + -0.8 3,183,186 + -2.3 3,183,186 + -0.6 3,183,187 - -1.4 3,183,187 - -1.5 3,183,187 - -1.4 3,183,187 - -0.1 3,183,187 - -1.4 3,183,187 - -0.5 3,183,187 - -0.6 3,183,187 - -0.4 3,183,187 - -1.2 3,183,187 - +0.2 3,183,187 - -1.1 3,183,187 - -2.3 3,183,191 - -1.6 3,183,191 - +0.3 3,183,191 - -0.1 3,183,240 + -1.3 3,183,240 + -1.1 3,183,240 + -1.7 3,183,241 - -0.1 3,183,241 - -0.8 3,183,241 - +0.7 3,183,241 - -1.0 3,183,241 - -2.1 3,183,241 - -0.8 3,183,269 - -2.5 3,183,320 + -0.3 3,183,320 + -1.7 3,183,321 - -0.8 3,183,321 - -0.4 3,183,321 - +1.2 3,183,321 - -0.4 3,183,321 - -2.4 3,183,326 + -2.0 3,183,327 - +1.2 3,183,424 + -0.9 3,183,424 + -0.5 3,183,424 + -1.7 3,183,425 - +0.1 3,183,425 - -0.1 3,183,425 - +0.9 3,183,425 - +0.0 3,183,427 - +0.6 3,183,477 + -0.3 3,183,477 + -0.3 3,183,477 + -0.8 3,183,477 + -0.5 3,183,478 - +1.4 3,183,478 - +1.0 3,183,478 - +0.5 3,183,478 - -1.1 3,183,478 - +0.3 3,183,478 - -1.7 3,183,478 - -0.2 3,183,480 + -0.7 3,183,480 + -0.7 3,183,480 + -3.2 3,183,481 - -0.5 3,183,481 - -1.1 3,183,481 - -0.5 3,183,481 - +0.0 3,183,484 + +1.9 3,183,845 + +0.4 3,183,846 - -0.8 3,183,874 - +0.9 3,184,133 + Pf6N2E2_2694 0.16 -0.1 3,184,308 - Pf6N2E2_2694 0.20 -0.1 3,184,844 + Pf6N2E2_2694 0.34 +1.4 3,184,845 - Pf6N2E2_2694 0.34 +0.7 3,184,845 - Pf6N2E2_2694 0.34 -1.4 3,184,845 - Pf6N2E2_2694 0.34 +1.5 3,184,845 - Pf6N2E2_2694 0.34 +0.7 3,185,036 + Pf6N2E2_2694 0.39 +0.3 3,185,037 - Pf6N2E2_2694 0.39 -0.1 3,185,045 + Pf6N2E2_2694 0.39 -3.3 3,185,045 + Pf6N2E2_2694 0.39 -0.5 3,185,046 - Pf6N2E2_2694 0.39 +0.3 3,185,046 - Pf6N2E2_2694 0.39 -1.4 3,185,276 + Pf6N2E2_2694 0.45 -0.9 3,185,276 + Pf6N2E2_2694 0.45 +0.7 3,185,277 - Pf6N2E2_2694 0.45 -0.1 3,185,277 - Pf6N2E2_2694 0.45 +0.8 3,185,393 + Pf6N2E2_2694 0.48 -2.3 3,185,394 - Pf6N2E2_2694 0.48 +0.4 3,185,394 - Pf6N2E2_2694 0.48 +0.4 3,185,667 - Pf6N2E2_2694 0.55 +1.1 3,185,667 - Pf6N2E2_2694 0.55 -0.3 3,185,667 - Pf6N2E2_2694 0.55 +0.8 3,185,667 - Pf6N2E2_2694 0.55 -0.1 3,185,667 - Pf6N2E2_2694 0.55 +1.2 3,186,179 + Pf6N2E2_2694 0.68 +0.4 3,186,180 - Pf6N2E2_2694 0.68 +0.3 3,186,288 - Pf6N2E2_2694 0.71 +1.1 3,186,444 - Pf6N2E2_2694 0.75 +0.6 3,186,444 - Pf6N2E2_2694 0.75 +1.7 3,186,444 - Pf6N2E2_2694 0.75 +1.3 3,186,480 + Pf6N2E2_2694 0.76 +0.2 3,186,570 - Pf6N2E2_2694 0.78 -0.1 3,186,878 + Pf6N2E2_2694 0.86 +1.2 3,187,439 + -1.7 3,187,439 + +0.8 3,187,440 - +0.3 3,187,575 + +0.7 3,187,575 + -0.2 3,187,575 + -0.7 3,187,576 - -0.1 3,187,576 - +0.6 3,187,576 - +0.6 3,187,576 - -0.4 3,187,591 + -0.2 3,187,591 + +0.0 3,187,591 + +0.3
Or see this region's nucleotide sequence