Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2645 and Pf6N2E2_2646 are separated by 433 nucleotides Pf6N2E2_2646 and Pf6N2E2_2647 are separated by 8 nucleotides Pf6N2E2_2647 and Pf6N2E2_2648 are separated by 140 nucleotides
Pf6N2E2_2645 - ATP-dependent DNA helicase RecQ, at 3,107,976 to 3,110,102
_2645
Pf6N2E2_2646 - FIG00953416: hypothetical protein, at 3,110,536 to 3,111,123
_2646
Pf6N2E2_2647 - FIG039061: hypothetical protein related to heme utilization, at 3,111,132 to 3,111,527
_2647
Pf6N2E2_2648 - T1SS secreted agglutinin RTX, at 3,111,668 to 3,120,229
_2648
Position (kb)
3110
3111
3112 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3109.642 kb on + strand, within Pf6N2E2_2645 at 3109.642 kb on + strand, within Pf6N2E2_2645 at 3109.643 kb on - strand, within Pf6N2E2_2645 at 3109.736 kb on + strand, within Pf6N2E2_2645 at 3109.736 kb on + strand, within Pf6N2E2_2645 at 3109.736 kb on + strand, within Pf6N2E2_2645 at 3109.737 kb on - strand, within Pf6N2E2_2645 at 3109.737 kb on - strand, within Pf6N2E2_2645 at 3109.737 kb on - strand, within Pf6N2E2_2645 at 3109.737 kb on - strand, within Pf6N2E2_2645 at 3109.737 kb on - strand, within Pf6N2E2_2645 at 3109.768 kb on + strand, within Pf6N2E2_2645 at 3109.769 kb on - strand, within Pf6N2E2_2645 at 3110.102 kb on + strand at 3110.102 kb on + strand at 3110.102 kb on + strand at 3110.102 kb on + strand at 3110.103 kb on - strand at 3110.103 kb on - strand at 3110.176 kb on - strand at 3110.176 kb on - strand at 3110.176 kb on - strand at 3110.229 kb on + strand at 3110.229 kb on + strand at 3110.414 kb on + strand at 3110.414 kb on + strand at 3110.414 kb on + strand at 3110.414 kb on + strand at 3110.415 kb on - strand at 3110.415 kb on - strand at 3110.415 kb on - strand at 3110.415 kb on - strand at 3110.415 kb on - strand at 3110.415 kb on - strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.462 kb on - strand at 3110.462 kb on - strand at 3110.462 kb on - strand at 3110.534 kb on + strand at 3110.612 kb on - strand, within Pf6N2E2_2646 at 3110.612 kb on - strand, within Pf6N2E2_2646 at 3110.630 kb on - strand, within Pf6N2E2_2646 at 3111.171 kb on + strand at 3111.300 kb on + strand, within Pf6N2E2_2647 at 3111.300 kb on + strand, within Pf6N2E2_2647 at 3111.300 kb on + strand, within Pf6N2E2_2647 at 3111.301 kb on - strand, within Pf6N2E2_2647 at 3111.301 kb on - strand, within Pf6N2E2_2647 at 3111.313 kb on + strand, within Pf6N2E2_2647 at 3111.313 kb on + strand, within Pf6N2E2_2647 at 3111.348 kb on + strand, within Pf6N2E2_2647 at 3111.349 kb on - strand, within Pf6N2E2_2647 at 3111.380 kb on + strand, within Pf6N2E2_2647 at 3111.387 kb on + strand, within Pf6N2E2_2647 at 3111.388 kb on - strand, within Pf6N2E2_2647 at 3111.388 kb on - strand, within Pf6N2E2_2647 at 3111.429 kb on + strand, within Pf6N2E2_2647 at 3111.492 kb on + strand at 3111.492 kb on + strand at 3111.492 kb on + strand at 3111.493 kb on - strand at 3111.493 kb on - strand at 3111.493 kb on - strand at 3111.493 kb on - strand at 3111.493 kb on - strand at 3111.494 kb on + strand at 3111.494 kb on + strand at 3111.495 kb on - strand at 3111.495 kb on - strand at 3111.495 kb on - strand at 3111.495 kb on - strand at 3111.655 kb on - strand at 3111.655 kb on - strand at 3111.853 kb on + strand at 3111.854 kb on - strand at 3111.854 kb on - strand at 3111.854 kb on - strand at 3111.895 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,109,642 + Pf6N2E2_2645 0.78 +0.8 3,109,642 + Pf6N2E2_2645 0.78 +1.7 3,109,643 - Pf6N2E2_2645 0.78 +2.3 3,109,736 + Pf6N2E2_2645 0.83 +0.6 3,109,736 + Pf6N2E2_2645 0.83 +1.5 3,109,736 + Pf6N2E2_2645 0.83 +1.8 3,109,737 - Pf6N2E2_2645 0.83 +1.5 3,109,737 - Pf6N2E2_2645 0.83 +1.1 3,109,737 - Pf6N2E2_2645 0.83 +0.7 3,109,737 - Pf6N2E2_2645 0.83 +0.3 3,109,737 - Pf6N2E2_2645 0.83 +1.3 3,109,768 + Pf6N2E2_2645 0.84 +1.9 3,109,769 - Pf6N2E2_2645 0.84 +1.3 3,110,102 + +2.1 3,110,102 + -0.4 3,110,102 + +1.8 3,110,102 + +2.3 3,110,103 - +1.2 3,110,103 - +1.6 3,110,176 - -0.1 3,110,176 - -2.0 3,110,176 - -3.1 3,110,229 + +2.1 3,110,229 + +0.8 3,110,414 + +0.7 3,110,414 + +0.1 3,110,414 + +0.3 3,110,414 + +1.0 3,110,415 - -2.8 3,110,415 - -4.2 3,110,415 - -0.3 3,110,415 - -4.2 3,110,415 - -2.8 3,110,415 - -3.3 3,110,416 + +0.1 3,110,416 + +0.8 3,110,416 + -1.4 3,110,416 + +0.4 3,110,416 + -0.6 3,110,416 + +0.3 3,110,416 + +0.7 3,110,416 + -2.4 3,110,416 + -0.5 3,110,416 + +0.5 3,110,416 + +0.7 3,110,416 + +1.2 3,110,416 + +1.2 3,110,416 + -0.1 3,110,416 + +0.7 3,110,416 + -0.1 3,110,416 + -0.4 3,110,417 - -2.3 3,110,417 - -3.3 3,110,417 - -1.4 3,110,417 - -3.1 3,110,417 - -4.9 3,110,417 - -4.0 3,110,417 - -3.0 3,110,417 - -1.1 3,110,417 - -3.0 3,110,417 - -2.9 3,110,417 - -2.8 3,110,417 - -3.1 3,110,417 - -3.3 3,110,417 - -1.3 3,110,417 - -3.0 3,110,417 - -2.6 3,110,417 - -3.8 3,110,417 - -3.6 3,110,462 - -3.7 3,110,462 - -2.1 3,110,462 - -2.4 3,110,534 + +3.0 3,110,612 - Pf6N2E2_2646 0.13 -2.4 3,110,612 - Pf6N2E2_2646 0.13 -0.7 3,110,630 - Pf6N2E2_2646 0.16 -1.4 3,111,171 + +0.4 3,111,300 + Pf6N2E2_2647 0.42 +0.5 3,111,300 + Pf6N2E2_2647 0.42 +0.5 3,111,300 + Pf6N2E2_2647 0.42 -0.1 3,111,301 - Pf6N2E2_2647 0.43 -1.7 3,111,301 - Pf6N2E2_2647 0.43 -0.8 3,111,313 + Pf6N2E2_2647 0.46 +0.8 3,111,313 + Pf6N2E2_2647 0.46 -0.1 3,111,348 + Pf6N2E2_2647 0.55 +0.3 3,111,349 - Pf6N2E2_2647 0.55 -1.2 3,111,380 + Pf6N2E2_2647 0.63 +0.9 3,111,387 + Pf6N2E2_2647 0.64 -0.6 3,111,388 - Pf6N2E2_2647 0.65 -1.1 3,111,388 - Pf6N2E2_2647 0.65 -0.8 3,111,429 + Pf6N2E2_2647 0.75 +1.2 3,111,492 + -0.1 3,111,492 + -0.3 3,111,492 + +0.6 3,111,493 - +0.3 3,111,493 - -0.1 3,111,493 - +0.1 3,111,493 - -0.4 3,111,493 - -2.4 3,111,494 + -0.1 3,111,494 + +0.1 3,111,495 - -0.5 3,111,495 - -1.6 3,111,495 - -0.6 3,111,495 - +0.6 3,111,655 - +0.8 3,111,655 - -1.6 3,111,853 + +1.3 3,111,854 - +0.2 3,111,854 - -0.7 3,111,854 - -0.0 3,111,895 - +1.7
Or see this region's nucleotide sequence