Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2643 and Pf6N2E2_2644 are separated by 113 nucleotides Pf6N2E2_2644 and Pf6N2E2_2645 are separated by 118 nucleotides Pf6N2E2_2645 and Pf6N2E2_2646 are separated by 433 nucleotides
Pf6N2E2_2643 - Tfp pilus assembly protein FimV, at 3,105,351 to 3,107,309
_2643
Pf6N2E2_2644 - Transcriptional regulator SlyA, at 3,107,423 to 3,107,857
_2644
Pf6N2E2_2645 - ATP-dependent DNA helicase RecQ, at 3,107,976 to 3,110,102
_2645
Pf6N2E2_2646 - FIG00953416: hypothetical protein, at 3,110,536 to 3,111,123
_2646
Position (kb)
3107
3108
3109
3110
3111 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3107.202 kb on + strand at 3107.202 kb on + strand at 3107.202 kb on + strand at 3107.202 kb on + strand at 3107.202 kb on + strand at 3107.202 kb on + strand at 3107.202 kb on + strand at 3107.202 kb on + strand at 3107.203 kb on - strand at 3107.341 kb on - strand at 3107.353 kb on + strand at 3107.363 kb on + strand at 3107.363 kb on + strand at 3107.363 kb on + strand at 3107.363 kb on + strand at 3107.363 kb on + strand at 3107.363 kb on + strand at 3107.363 kb on + strand at 3107.364 kb on - strand at 3107.364 kb on - strand at 3107.364 kb on - strand at 3107.364 kb on - strand at 3107.364 kb on - strand at 3107.364 kb on - strand at 3107.364 kb on - strand at 3107.364 kb on - strand at 3107.364 kb on - strand at 3107.367 kb on - strand at 3107.374 kb on - strand at 3107.375 kb on + strand at 3107.375 kb on + strand at 3107.375 kb on + strand at 3107.375 kb on + strand at 3107.376 kb on - strand at 3107.376 kb on - strand at 3107.376 kb on - strand at 3107.417 kb on + strand at 3107.417 kb on + strand at 3107.858 kb on - strand at 3107.944 kb on + strand at 3107.971 kb on - strand at 3108.075 kb on + strand at 3108.193 kb on + strand, within Pf6N2E2_2645 at 3108.193 kb on + strand, within Pf6N2E2_2645 at 3108.193 kb on + strand, within Pf6N2E2_2645 at 3108.279 kb on + strand, within Pf6N2E2_2645 at 3108.280 kb on - strand, within Pf6N2E2_2645 at 3108.321 kb on + strand, within Pf6N2E2_2645 at 3108.322 kb on - strand, within Pf6N2E2_2645 at 3108.451 kb on + strand, within Pf6N2E2_2645 at 3108.451 kb on + strand, within Pf6N2E2_2645 at 3108.452 kb on - strand, within Pf6N2E2_2645 at 3108.659 kb on - strand, within Pf6N2E2_2645 at 3108.709 kb on - strand, within Pf6N2E2_2645 at 3108.808 kb on - strand, within Pf6N2E2_2645 at 3109.166 kb on - strand, within Pf6N2E2_2645 at 3109.166 kb on - strand, within Pf6N2E2_2645 at 3109.166 kb on - strand, within Pf6N2E2_2645 at 3109.168 kb on + strand, within Pf6N2E2_2645 at 3109.168 kb on + strand, within Pf6N2E2_2645 at 3109.168 kb on + strand, within Pf6N2E2_2645 at 3109.169 kb on - strand, within Pf6N2E2_2645 at 3109.402 kb on + strand, within Pf6N2E2_2645 at 3109.402 kb on + strand, within Pf6N2E2_2645 at 3109.474 kb on - strand, within Pf6N2E2_2645 at 3109.642 kb on + strand, within Pf6N2E2_2645 at 3109.642 kb on + strand, within Pf6N2E2_2645 at 3109.643 kb on - strand, within Pf6N2E2_2645 at 3109.736 kb on + strand, within Pf6N2E2_2645 at 3109.736 kb on + strand, within Pf6N2E2_2645 at 3109.736 kb on + strand, within Pf6N2E2_2645 at 3109.737 kb on - strand, within Pf6N2E2_2645 at 3109.737 kb on - strand, within Pf6N2E2_2645 at 3109.737 kb on - strand, within Pf6N2E2_2645 at 3109.737 kb on - strand, within Pf6N2E2_2645 at 3109.737 kb on - strand, within Pf6N2E2_2645 at 3109.768 kb on + strand, within Pf6N2E2_2645 at 3109.769 kb on - strand, within Pf6N2E2_2645 at 3110.102 kb on + strand at 3110.102 kb on + strand at 3110.102 kb on + strand at 3110.102 kb on + strand at 3110.103 kb on - strand at 3110.103 kb on - strand at 3110.176 kb on - strand at 3110.176 kb on - strand at 3110.176 kb on - strand at 3110.229 kb on + strand at 3110.229 kb on + strand at 3110.414 kb on + strand at 3110.414 kb on + strand at 3110.414 kb on + strand at 3110.414 kb on + strand at 3110.415 kb on - strand at 3110.415 kb on - strand at 3110.415 kb on - strand at 3110.415 kb on - strand at 3110.415 kb on - strand at 3110.415 kb on - strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.416 kb on + strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.417 kb on - strand at 3110.462 kb on - strand at 3110.462 kb on - strand at 3110.462 kb on - strand at 3110.534 kb on + strand at 3110.612 kb on - strand, within Pf6N2E2_2646 at 3110.612 kb on - strand, within Pf6N2E2_2646 at 3110.630 kb on - strand, within Pf6N2E2_2646
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,107,202 + +1.5 3,107,202 + +1.9 3,107,202 + -2.7 3,107,202 + -0.0 3,107,202 + +1.1 3,107,202 + +0.7 3,107,202 + +1.5 3,107,202 + +1.5 3,107,203 - +0.6 3,107,341 - +1.5 3,107,353 + +2.7 3,107,363 + +0.9 3,107,363 + +2.8 3,107,363 + +1.3 3,107,363 + +0.8 3,107,363 + +1.1 3,107,363 + +1.5 3,107,363 + +1.2 3,107,364 - +1.2 3,107,364 - +1.7 3,107,364 - +1.2 3,107,364 - +0.2 3,107,364 - +2.0 3,107,364 - +1.9 3,107,364 - +0.8 3,107,364 - -0.4 3,107,364 - +1.5 3,107,367 - +0.9 3,107,374 - +1.7 3,107,375 + +0.8 3,107,375 + +0.9 3,107,375 + -0.1 3,107,375 + +0.7 3,107,376 - +0.7 3,107,376 - +2.1 3,107,376 - -0.9 3,107,417 + +3.0 3,107,417 + +1.6 3,107,858 - +3.1 3,107,944 + +4.1 3,107,971 - -1.1 3,108,075 + +2.7 3,108,193 + Pf6N2E2_2645 0.10 +3.8 3,108,193 + Pf6N2E2_2645 0.10 +4.2 3,108,193 + Pf6N2E2_2645 0.10 +2.7 3,108,279 + Pf6N2E2_2645 0.14 +1.6 3,108,280 - Pf6N2E2_2645 0.14 -3.8 3,108,321 + Pf6N2E2_2645 0.16 +2.7 3,108,322 - Pf6N2E2_2645 0.16 -3.4 3,108,451 + Pf6N2E2_2645 0.22 +1.3 3,108,451 + Pf6N2E2_2645 0.22 +2.0 3,108,452 - Pf6N2E2_2645 0.22 -4.2 3,108,659 - Pf6N2E2_2645 0.32 -3.3 3,108,709 - Pf6N2E2_2645 0.34 -3.2 3,108,808 - Pf6N2E2_2645 0.39 -4.0 3,109,166 - Pf6N2E2_2645 0.56 -1.3 3,109,166 - Pf6N2E2_2645 0.56 -2.1 3,109,166 - Pf6N2E2_2645 0.56 -2.1 3,109,168 + Pf6N2E2_2645 0.56 +2.8 3,109,168 + Pf6N2E2_2645 0.56 +1.6 3,109,168 + Pf6N2E2_2645 0.56 +2.1 3,109,169 - Pf6N2E2_2645 0.56 -0.8 3,109,402 + Pf6N2E2_2645 0.67 +2.0 3,109,402 + Pf6N2E2_2645 0.67 -0.5 3,109,474 - Pf6N2E2_2645 0.70 +0.9 3,109,642 + Pf6N2E2_2645 0.78 +0.8 3,109,642 + Pf6N2E2_2645 0.78 +1.7 3,109,643 - Pf6N2E2_2645 0.78 +2.3 3,109,736 + Pf6N2E2_2645 0.83 +0.6 3,109,736 + Pf6N2E2_2645 0.83 +1.5 3,109,736 + Pf6N2E2_2645 0.83 +1.8 3,109,737 - Pf6N2E2_2645 0.83 +1.5 3,109,737 - Pf6N2E2_2645 0.83 +1.1 3,109,737 - Pf6N2E2_2645 0.83 +0.7 3,109,737 - Pf6N2E2_2645 0.83 +0.3 3,109,737 - Pf6N2E2_2645 0.83 +1.3 3,109,768 + Pf6N2E2_2645 0.84 +1.9 3,109,769 - Pf6N2E2_2645 0.84 +1.3 3,110,102 + +2.1 3,110,102 + -0.4 3,110,102 + +1.8 3,110,102 + +2.3 3,110,103 - +1.2 3,110,103 - +1.6 3,110,176 - -0.1 3,110,176 - -2.0 3,110,176 - -3.1 3,110,229 + +2.1 3,110,229 + +0.8 3,110,414 + +0.7 3,110,414 + +0.1 3,110,414 + +0.3 3,110,414 + +1.0 3,110,415 - -2.8 3,110,415 - -4.2 3,110,415 - -0.3 3,110,415 - -4.2 3,110,415 - -2.8 3,110,415 - -3.3 3,110,416 + +0.1 3,110,416 + +0.8 3,110,416 + -1.4 3,110,416 + +0.4 3,110,416 + -0.6 3,110,416 + +0.3 3,110,416 + +0.7 3,110,416 + -2.4 3,110,416 + -0.5 3,110,416 + +0.5 3,110,416 + +0.7 3,110,416 + +1.2 3,110,416 + +1.2 3,110,416 + -0.1 3,110,416 + +0.7 3,110,416 + -0.1 3,110,416 + -0.4 3,110,417 - -2.3 3,110,417 - -3.3 3,110,417 - -1.4 3,110,417 - -3.1 3,110,417 - -4.9 3,110,417 - -4.0 3,110,417 - -3.0 3,110,417 - -1.1 3,110,417 - -3.0 3,110,417 - -2.9 3,110,417 - -2.8 3,110,417 - -3.1 3,110,417 - -3.3 3,110,417 - -1.3 3,110,417 - -3.0 3,110,417 - -2.6 3,110,417 - -3.8 3,110,417 - -3.6 3,110,462 - -3.7 3,110,462 - -2.1 3,110,462 - -2.4 3,110,534 + +3.0 3,110,612 - Pf6N2E2_2646 0.13 -2.4 3,110,612 - Pf6N2E2_2646 0.13 -0.7 3,110,630 - Pf6N2E2_2646 0.16 -1.4
Or see this region's nucleotide sequence