Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2640 and Pf6N2E2_2641 overlap by 7 nucleotides Pf6N2E2_2641 and Pf6N2E2_2642 are separated by 100 nucleotides Pf6N2E2_2642 and Pf6N2E2_2643 are separated by 139 nucleotides
Pf6N2E2_2640 - Permease of the drug/metabolite transporter (DMT) superfamily, at 3,101,768 to 3,102,658
_2640
Pf6N2E2_2641 - SelT/selW/selH selenoprotein domain, at 3,102,652 to 3,102,939
_2641
Pf6N2E2_2642 - putative patatin-like phospholipase, at 3,103,040 to 3,105,211
_2642
Pf6N2E2_2643 - Tfp pilus assembly protein FimV, at 3,105,351 to 3,107,309
_2643
Position (kb)
3103
3104
3105
3106 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3102.506 kb on + strand, within Pf6N2E2_2640 at 3102.506 kb on + strand, within Pf6N2E2_2640 at 3102.771 kb on + strand, within Pf6N2E2_2641 at 3102.771 kb on + strand, within Pf6N2E2_2641 at 3102.905 kb on + strand, within Pf6N2E2_2641 at 3102.905 kb on + strand, within Pf6N2E2_2641 at 3102.905 kb on + strand, within Pf6N2E2_2641 at 3102.905 kb on + strand, within Pf6N2E2_2641 at 3102.906 kb on - strand, within Pf6N2E2_2641 at 3102.906 kb on - strand, within Pf6N2E2_2641 at 3102.906 kb on - strand, within Pf6N2E2_2641 at 3102.906 kb on - strand, within Pf6N2E2_2641 at 3102.906 kb on - strand, within Pf6N2E2_2641 at 3102.906 kb on - strand, within Pf6N2E2_2641 at 3102.927 kb on + strand at 3102.928 kb on - strand at 3102.972 kb on + strand at 3102.972 kb on + strand at 3102.972 kb on + strand at 3102.973 kb on - strand at 3102.973 kb on - strand at 3102.973 kb on - strand at 3102.974 kb on + strand at 3102.974 kb on + strand at 3102.974 kb on + strand at 3102.974 kb on + strand at 3102.974 kb on + strand at 3102.975 kb on - strand at 3102.975 kb on - strand at 3102.975 kb on - strand at 3102.975 kb on - strand at 3102.975 kb on - strand at 3102.978 kb on - strand at 3103.230 kb on + strand at 3103.230 kb on + strand at 3103.230 kb on + strand at 3103.230 kb on + strand at 3103.230 kb on + strand at 3103.231 kb on - strand at 3103.231 kb on - strand at 3103.231 kb on - strand at 3103.231 kb on - strand at 3103.232 kb on + strand at 3103.232 kb on + strand at 3103.232 kb on + strand at 3103.232 kb on + strand at 3103.232 kb on + strand at 3103.232 kb on + strand at 3103.232 kb on + strand at 3103.232 kb on + strand at 3103.232 kb on + strand at 3103.232 kb on + strand at 3103.233 kb on - strand at 3103.233 kb on - strand at 3103.233 kb on - strand at 3103.233 kb on - strand at 3103.233 kb on - strand at 3103.244 kb on + strand at 3103.245 kb on - strand at 3103.245 kb on - strand at 3103.245 kb on - strand at 3103.245 kb on - strand at 3103.326 kb on + strand, within Pf6N2E2_2642 at 3103.326 kb on + strand, within Pf6N2E2_2642 at 3103.327 kb on - strand, within Pf6N2E2_2642 at 3103.477 kb on + strand, within Pf6N2E2_2642 at 3103.477 kb on + strand, within Pf6N2E2_2642 at 3103.478 kb on - strand, within Pf6N2E2_2642 at 3103.478 kb on - strand, within Pf6N2E2_2642 at 3103.478 kb on - strand, within Pf6N2E2_2642 at 3103.478 kb on - strand, within Pf6N2E2_2642 at 3103.478 kb on - strand, within Pf6N2E2_2642 at 3103.959 kb on + strand, within Pf6N2E2_2642 at 3104.084 kb on + strand, within Pf6N2E2_2642 at 3104.084 kb on + strand, within Pf6N2E2_2642 at 3104.085 kb on - strand, within Pf6N2E2_2642 at 3104.085 kb on - strand, within Pf6N2E2_2642 at 3104.150 kb on + strand, within Pf6N2E2_2642 at 3104.150 kb on + strand, within Pf6N2E2_2642 at 3104.150 kb on + strand, within Pf6N2E2_2642 at 3104.151 kb on - strand, within Pf6N2E2_2642 at 3104.162 kb on + strand, within Pf6N2E2_2642 at 3104.162 kb on + strand, within Pf6N2E2_2642 at 3104.396 kb on + strand, within Pf6N2E2_2642 at 3104.396 kb on + strand, within Pf6N2E2_2642 at 3104.396 kb on + strand, within Pf6N2E2_2642 at 3104.408 kb on + strand, within Pf6N2E2_2642 at 3104.409 kb on - strand, within Pf6N2E2_2642 at 3104.409 kb on - strand, within Pf6N2E2_2642 at 3104.409 kb on - strand, within Pf6N2E2_2642 at 3104.436 kb on - strand, within Pf6N2E2_2642 at 3104.436 kb on - strand, within Pf6N2E2_2642 at 3104.663 kb on + strand, within Pf6N2E2_2642 at 3104.664 kb on - strand, within Pf6N2E2_2642 at 3104.702 kb on + strand, within Pf6N2E2_2642 at 3104.702 kb on + strand, within Pf6N2E2_2642 at 3104.702 kb on + strand, within Pf6N2E2_2642 at 3104.703 kb on - strand, within Pf6N2E2_2642 at 3104.703 kb on - strand, within Pf6N2E2_2642 at 3104.748 kb on - strand, within Pf6N2E2_2642 at 3104.748 kb on - strand, within Pf6N2E2_2642 at 3104.975 kb on + strand, within Pf6N2E2_2642 at 3104.975 kb on + strand, within Pf6N2E2_2642 at 3104.976 kb on - strand, within Pf6N2E2_2642 at 3105.002 kb on + strand at 3105.026 kb on + strand at 3105.026 kb on + strand at 3105.027 kb on - strand at 3105.027 kb on - strand at 3105.027 kb on - strand at 3105.089 kb on + strand at 3105.089 kb on + strand at 3105.090 kb on - strand at 3105.090 kb on - strand at 3105.124 kb on + strand at 3105.125 kb on - strand at 3105.125 kb on - strand at 3105.152 kb on + strand at 3105.152 kb on + strand at 3105.152 kb on + strand at 3105.153 kb on - strand at 3105.153 kb on - strand at 3105.153 kb on - strand at 3105.153 kb on - strand at 3105.185 kb on + strand at 3105.185 kb on + strand at 3105.185 kb on + strand at 3105.186 kb on - strand at 3105.688 kb on + strand, within Pf6N2E2_2643 at 3105.833 kb on + strand, within Pf6N2E2_2643 at 3105.834 kb on - strand, within Pf6N2E2_2643 at 3105.954 kb on + strand, within Pf6N2E2_2643 at 3105.954 kb on + strand, within Pf6N2E2_2643 at 3105.954 kb on + strand, within Pf6N2E2_2643 at 3105.954 kb on + strand, within Pf6N2E2_2643 at 3105.955 kb on - strand, within Pf6N2E2_2643 at 3105.955 kb on - strand, within Pf6N2E2_2643 at 3105.955 kb on - strand, within Pf6N2E2_2643 at 3106.096 kb on + strand, within Pf6N2E2_2643 at 3106.097 kb on - strand, within Pf6N2E2_2643 at 3106.097 kb on - strand, within Pf6N2E2_2643 at 3106.097 kb on - strand, within Pf6N2E2_2643 at 3106.105 kb on + strand, within Pf6N2E2_2643 at 3106.105 kb on + strand, within Pf6N2E2_2643 at 3106.152 kb on - strand, within Pf6N2E2_2643
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,102,506 + Pf6N2E2_2640 0.83 -0.7 3,102,506 + Pf6N2E2_2640 0.83 -0.2 3,102,771 + Pf6N2E2_2641 0.41 +0.4 3,102,771 + Pf6N2E2_2641 0.41 +1.0 3,102,905 + Pf6N2E2_2641 0.88 +2.4 3,102,905 + Pf6N2E2_2641 0.88 +0.4 3,102,905 + Pf6N2E2_2641 0.88 +2.6 3,102,905 + Pf6N2E2_2641 0.88 +1.9 3,102,906 - Pf6N2E2_2641 0.88 -0.5 3,102,906 - Pf6N2E2_2641 0.88 -1.4 3,102,906 - Pf6N2E2_2641 0.88 +0.3 3,102,906 - Pf6N2E2_2641 0.88 -1.0 3,102,906 - Pf6N2E2_2641 0.88 -1.4 3,102,906 - Pf6N2E2_2641 0.88 -1.1 3,102,927 + +1.4 3,102,928 - +1.1 3,102,972 + +2.2 3,102,972 + +1.4 3,102,972 + +1.0 3,102,973 - -0.5 3,102,973 - +0.2 3,102,973 - +1.0 3,102,974 + +1.9 3,102,974 + +1.4 3,102,974 + +0.2 3,102,974 + -0.0 3,102,974 + +0.9 3,102,975 - -2.7 3,102,975 - +0.9 3,102,975 - -0.5 3,102,975 - -3.7 3,102,975 - -1.7 3,102,978 - -1.7 3,103,230 + -1.3 3,103,230 + -4.6 3,103,230 + -1.8 3,103,230 + -1.7 3,103,230 + -2.3 3,103,231 - -4.3 3,103,231 - -2.1 3,103,231 - -2.3 3,103,231 - -0.1 3,103,232 + -3.4 3,103,232 + -3.9 3,103,232 + -2.7 3,103,232 + -1.6 3,103,232 + -0.3 3,103,232 + -1.5 3,103,232 + -2.6 3,103,232 + -2.8 3,103,232 + -0.9 3,103,232 + -2.3 3,103,233 - -1.9 3,103,233 - -2.8 3,103,233 - -1.5 3,103,233 - -2.9 3,103,233 - -1.8 3,103,244 + -2.5 3,103,245 - -2.7 3,103,245 - -3.9 3,103,245 - -2.7 3,103,245 - -3.1 3,103,326 + Pf6N2E2_2642 0.13 -3.9 3,103,326 + Pf6N2E2_2642 0.13 -1.6 3,103,327 - Pf6N2E2_2642 0.13 -1.4 3,103,477 + Pf6N2E2_2642 0.20 -5.0 3,103,477 + Pf6N2E2_2642 0.20 -2.3 3,103,478 - Pf6N2E2_2642 0.20 -2.6 3,103,478 - Pf6N2E2_2642 0.20 -2.0 3,103,478 - Pf6N2E2_2642 0.20 -0.2 3,103,478 - Pf6N2E2_2642 0.20 -1.7 3,103,478 - Pf6N2E2_2642 0.20 -1.1 3,103,959 + Pf6N2E2_2642 0.42 -1.1 3,104,084 + Pf6N2E2_2642 0.48 -2.4 3,104,084 + Pf6N2E2_2642 0.48 -1.1 3,104,085 - Pf6N2E2_2642 0.48 -4.4 3,104,085 - Pf6N2E2_2642 0.48 -2.6 3,104,150 + Pf6N2E2_2642 0.51 -0.3 3,104,150 + Pf6N2E2_2642 0.51 -3.7 3,104,150 + Pf6N2E2_2642 0.51 +0.7 3,104,151 - Pf6N2E2_2642 0.51 -1.4 3,104,162 + Pf6N2E2_2642 0.52 -0.5 3,104,162 + Pf6N2E2_2642 0.52 -2.4 3,104,396 + Pf6N2E2_2642 0.62 -1.9 3,104,396 + Pf6N2E2_2642 0.62 -1.3 3,104,396 + Pf6N2E2_2642 0.62 -3.3 3,104,408 + Pf6N2E2_2642 0.63 -1.1 3,104,409 - Pf6N2E2_2642 0.63 -1.5 3,104,409 - Pf6N2E2_2642 0.63 -2.9 3,104,409 - Pf6N2E2_2642 0.63 +0.3 3,104,436 - Pf6N2E2_2642 0.64 -0.8 3,104,436 - Pf6N2E2_2642 0.64 -1.2 3,104,663 + Pf6N2E2_2642 0.75 -0.2 3,104,664 - Pf6N2E2_2642 0.75 -2.9 3,104,702 + Pf6N2E2_2642 0.77 -3.3 3,104,702 + Pf6N2E2_2642 0.77 -2.9 3,104,702 + Pf6N2E2_2642 0.77 -1.7 3,104,703 - Pf6N2E2_2642 0.77 -1.0 3,104,703 - Pf6N2E2_2642 0.77 -1.7 3,104,748 - Pf6N2E2_2642 0.79 -2.8 3,104,748 - Pf6N2E2_2642 0.79 -3.3 3,104,975 + Pf6N2E2_2642 0.89 -1.3 3,104,975 + Pf6N2E2_2642 0.89 -2.6 3,104,976 - Pf6N2E2_2642 0.89 -1.1 3,105,002 + -2.4 3,105,026 + -2.3 3,105,026 + -0.4 3,105,027 - -3.0 3,105,027 - -1.3 3,105,027 - -1.6 3,105,089 + +0.5 3,105,089 + -2.1 3,105,090 - -2.8 3,105,090 - -2.7 3,105,124 + -2.9 3,105,125 - -2.4 3,105,125 - -3.6 3,105,152 + -1.2 3,105,152 + -2.7 3,105,152 + -1.5 3,105,153 - -4.2 3,105,153 - -3.3 3,105,153 - -2.1 3,105,153 - -0.9 3,105,185 + -1.1 3,105,185 + -0.8 3,105,185 + -2.7 3,105,186 - -3.8 3,105,688 + Pf6N2E2_2643 0.17 -0.3 3,105,833 + Pf6N2E2_2643 0.25 -0.1 3,105,834 - Pf6N2E2_2643 0.25 +0.6 3,105,954 + Pf6N2E2_2643 0.31 +0.5 3,105,954 + Pf6N2E2_2643 0.31 -1.4 3,105,954 + Pf6N2E2_2643 0.31 +1.1 3,105,954 + Pf6N2E2_2643 0.31 +0.1 3,105,955 - Pf6N2E2_2643 0.31 -0.0 3,105,955 - Pf6N2E2_2643 0.31 +0.6 3,105,955 - Pf6N2E2_2643 0.31 -1.0 3,106,096 + Pf6N2E2_2643 0.38 +1.3 3,106,097 - Pf6N2E2_2643 0.38 +0.9 3,106,097 - Pf6N2E2_2643 0.38 +0.3 3,106,097 - Pf6N2E2_2643 0.38 -0.2 3,106,105 + Pf6N2E2_2643 0.38 +1.1 3,106,105 + Pf6N2E2_2643 0.38 +0.4 3,106,152 - Pf6N2E2_2643 0.41 +0.3
Or see this region's nucleotide sequence