Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2516 and Pf6N2E2_2517 are separated by 120 nucleotides Pf6N2E2_2517 and Pf6N2E2_2518 overlap by 4 nucleotides Pf6N2E2_2518 and Pf6N2E2_2519 are separated by 28 nucleotides
Pf6N2E2_2516 - SSU ribosomal protein S1p, at 2,968,390 to 2,970,084
_2516
Pf6N2E2_2517 - Cytidylate kinase (EC 2.7.4.25), at 2,970,205 to 2,970,894
_2517
Pf6N2E2_2518 - Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19), at 2,970,891 to 2,973,098
_2518
Pf6N2E2_2519 - Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57), at 2,973,127 to 2,974,239
_2519
Position (kb)
2970
2971
2972
2973
2974 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2970.177 kb on - strand at 2972.225 kb on - strand, within Pf6N2E2_2518 at 2972.225 kb on - strand, within Pf6N2E2_2518 at 2972.355 kb on - strand, within Pf6N2E2_2518 at 2972.355 kb on - strand, within Pf6N2E2_2518 at 2973.099 kb on - strand at 2973.099 kb on - strand at 2973.191 kb on - strand at 2973.191 kb on - strand at 2973.222 kb on - strand at 2973.222 kb on - strand at 2973.222 kb on - strand at 2973.222 kb on - strand at 2973.222 kb on - strand at 2973.226 kb on - strand at 2973.226 kb on - strand at 2973.546 kb on - strand, within Pf6N2E2_2519
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,970,177 - -3.0 2,972,225 - Pf6N2E2_2518 0.60 +4.5 2,972,225 - Pf6N2E2_2518 0.60 +5.2 2,972,355 - Pf6N2E2_2518 0.66 -0.6 2,972,355 - Pf6N2E2_2518 0.66 -2.0 2,973,099 - +0.2 2,973,099 - +0.1 2,973,191 - +0.2 2,973,191 - +0.9 2,973,222 - -0.1 2,973,222 - +2.1 2,973,222 - +0.9 2,973,222 - +1.8 2,973,222 - -0.7 2,973,226 - +0.3 2,973,226 - +1.3 2,973,546 - Pf6N2E2_2519 0.38 +2.6
Or see this region's nucleotide sequence