Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2514

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2513 and Pf6N2E2_2514 are separated by 168 nucleotidesPf6N2E2_2514 and Pf6N2E2_2515 are separated by 430 nucleotides Pf6N2E2_2513 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 2,964,520 to 2,965,803 _2513 Pf6N2E2_2514 - regulator of length of O-antigen component of lipopolysaccharide chains, at 2,965,972 to 2,967,021 _2514 Pf6N2E2_2515 - Integration host factor beta subunit, at 2,967,452 to 2,967,748 _2515 Position (kb) 2965 2966 2967 2968Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2964.996 kb on - strand, within Pf6N2E2_2513at 2965.015 kb on - strand, within Pf6N2E2_2513at 2965.196 kb on - strand, within Pf6N2E2_2513at 2965.713 kb on - strandat 2965.862 kb on - strandat 2965.865 kb on - strandat 2965.865 kb on - strandat 2965.885 kb on - strandat 2965.885 kb on - strandat 2965.939 kb on - strandat 2965.944 kb on - strandat 2966.008 kb on - strandat 2966.057 kb on - strandat 2966.108 kb on - strand, within Pf6N2E2_2514at 2966.305 kb on - strand, within Pf6N2E2_2514at 2966.344 kb on - strand, within Pf6N2E2_2514at 2966.381 kb on - strand, within Pf6N2E2_2514at 2966.479 kb on + strand, within Pf6N2E2_2514at 2966.480 kb on - strand, within Pf6N2E2_2514at 2966.491 kb on - strand, within Pf6N2E2_2514at 2966.528 kb on + strand, within Pf6N2E2_2514at 2966.585 kb on + strand, within Pf6N2E2_2514at 2966.586 kb on - strand, within Pf6N2E2_2514at 2966.693 kb on - strand, within Pf6N2E2_2514at 2967.125 kb on + strandat 2967.163 kb on + strandat 2967.163 kb on + strandat 2967.164 kb on - strandat 2967.203 kb on - strandat 2967.252 kb on + strandat 2967.252 kb on + strandat 2967.257 kb on + strandat 2967.327 kb on + strandat 2967.328 kb on - strandat 2967.328 kb on - strandat 2967.328 kb on - strandat 2967.328 kb on - strandat 2967.491 kb on - strand, within Pf6N2E2_2515at 2967.799 kb on - strandat 2967.830 kb on + strandat 2967.947 kb on - strandat 2967.992 kb on - strandat 2967.992 kb on - strandat 2968.004 kb on + strandat 2968.005 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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2,964,996 - Pf6N2E2_2513 0.37 +0.8
2,965,015 - Pf6N2E2_2513 0.39 +1.8
2,965,196 - Pf6N2E2_2513 0.53 +3.1
2,965,713 - +3.5
2,965,862 - -0.4
2,965,865 - +0.0
2,965,865 - -1.9
2,965,885 - -1.9
2,965,885 - -2.0
2,965,939 - -1.7
2,965,944 - -0.6
2,966,008 - -1.6
2,966,057 - -2.5
2,966,108 - Pf6N2E2_2514 0.13 -3.2
2,966,305 - Pf6N2E2_2514 0.32 -2.6
2,966,344 - Pf6N2E2_2514 0.35 -2.0
2,966,381 - Pf6N2E2_2514 0.39 -1.3
2,966,479 + Pf6N2E2_2514 0.48 +1.4
2,966,480 - Pf6N2E2_2514 0.48 -4.0
2,966,491 - Pf6N2E2_2514 0.49 -2.3
2,966,528 + Pf6N2E2_2514 0.53 +1.3
2,966,585 + Pf6N2E2_2514 0.58 +3.0
2,966,586 - Pf6N2E2_2514 0.58 -2.7
2,966,693 - Pf6N2E2_2514 0.69 -3.3
2,967,125 + +2.8
2,967,163 + +1.8
2,967,163 + +0.8
2,967,164 - +1.1
2,967,203 - +0.0
2,967,252 + +2.0
2,967,252 + +2.1
2,967,257 + +1.8
2,967,327 + +0.7
2,967,328 - +0.1
2,967,328 - -0.0
2,967,328 - +0.9
2,967,328 - +0.5
2,967,491 - Pf6N2E2_2515 0.13 -0.5
2,967,799 - -1.4
2,967,830 + -0.7
2,967,947 - +0.3
2,967,992 - +1.4
2,967,992 - +0.4
2,968,004 + +0.6
2,968,005 - -1.6

Or see this region's nucleotide sequence