Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_247 and Pf6N2E2_248 are separated by 117 nucleotides Pf6N2E2_248 and Pf6N2E2_249 are separated by 384 nucleotides
Pf6N2E2_247 - Transcriptional regulator, AraC family, at 308,616 to 309,659
_247
Pf6N2E2_248 - hypothetical protein, at 309,777 to 310,190
_248
Pf6N2E2_249 - Pirin, at 310,575 to 311,297
_249
Position (kb)
309
310
311 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 308.802 kb on + strand, within Pf6N2E2_247 at 308.802 kb on + strand, within Pf6N2E2_247 at 308.803 kb on - strand, within Pf6N2E2_247 at 309.135 kb on + strand, within Pf6N2E2_247 at 309.135 kb on + strand, within Pf6N2E2_247 at 309.136 kb on - strand, within Pf6N2E2_247 at 309.136 kb on - strand, within Pf6N2E2_247 at 309.367 kb on + strand, within Pf6N2E2_247 at 309.367 kb on + strand, within Pf6N2E2_247 at 309.471 kb on + strand, within Pf6N2E2_247 at 309.474 kb on + strand, within Pf6N2E2_247 at 309.618 kb on - strand at 309.622 kb on - strand at 309.726 kb on + strand at 309.726 kb on + strand at 309.772 kb on - strand at 309.772 kb on - strand at 309.772 kb on - strand at 309.772 kb on - strand at 309.772 kb on - strand at 309.785 kb on + strand at 309.958 kb on + strand, within Pf6N2E2_248 at 309.958 kb on + strand, within Pf6N2E2_248 at 309.958 kb on + strand, within Pf6N2E2_248 at 309.958 kb on + strand, within Pf6N2E2_248 at 309.958 kb on + strand, within Pf6N2E2_248 at 309.958 kb on + strand, within Pf6N2E2_248 at 309.959 kb on - strand, within Pf6N2E2_248 at 309.959 kb on - strand, within Pf6N2E2_248 at 309.959 kb on - strand, within Pf6N2E2_248 at 309.959 kb on - strand, within Pf6N2E2_248 at 309.959 kb on - strand, within Pf6N2E2_248 at 309.959 kb on - strand, within Pf6N2E2_248 at 309.959 kb on - strand, within Pf6N2E2_248 at 310.013 kb on - strand, within Pf6N2E2_248 at 310.024 kb on + strand, within Pf6N2E2_248 at 310.025 kb on - strand, within Pf6N2E2_248 at 310.025 kb on - strand, within Pf6N2E2_248 at 310.025 kb on - strand, within Pf6N2E2_248 at 310.041 kb on + strand, within Pf6N2E2_248 at 310.042 kb on - strand, within Pf6N2E2_248 at 310.042 kb on - strand, within Pf6N2E2_248 at 310.090 kb on + strand, within Pf6N2E2_248 at 310.090 kb on + strand, within Pf6N2E2_248 at 310.090 kb on + strand, within Pf6N2E2_248 at 310.111 kb on + strand, within Pf6N2E2_248 at 310.111 kb on + strand, within Pf6N2E2_248 at 310.112 kb on - strand, within Pf6N2E2_248 at 310.112 kb on - strand, within Pf6N2E2_248 at 310.117 kb on + strand, within Pf6N2E2_248 at 310.117 kb on + strand, within Pf6N2E2_248 at 310.117 kb on + strand, within Pf6N2E2_248 at 310.117 kb on + strand, within Pf6N2E2_248 at 310.118 kb on - strand, within Pf6N2E2_248 at 310.158 kb on - strand at 310.176 kb on + strand at 310.177 kb on - strand at 310.177 kb on - strand at 310.177 kb on - strand at 310.179 kb on - strand at 310.206 kb on + strand at 310.207 kb on - strand at 310.227 kb on + strand at 310.328 kb on + strand at 310.393 kb on - strand at 310.449 kb on + strand at 310.450 kb on - strand at 310.450 kb on - strand at 310.469 kb on + strand at 310.469 kb on + strand at 310.469 kb on + strand at 310.509 kb on + strand at 310.510 kb on - strand at 310.517 kb on + strand at 310.517 kb on + strand at 310.518 kb on - strand at 310.526 kb on + strand at 310.699 kb on - strand, within Pf6N2E2_249 at 310.738 kb on + strand, within Pf6N2E2_249 at 310.738 kb on + strand, within Pf6N2E2_249 at 310.739 kb on - strand, within Pf6N2E2_249 at 310.739 kb on - strand, within Pf6N2E2_249 at 310.739 kb on - strand, within Pf6N2E2_249 at 310.739 kb on - strand, within Pf6N2E2_249 at 310.744 kb on + strand, within Pf6N2E2_249 at 310.745 kb on - strand, within Pf6N2E2_249 at 310.745 kb on - strand, within Pf6N2E2_249 at 310.802 kb on - strand, within Pf6N2E2_249 at 311.098 kb on + strand, within Pf6N2E2_249 at 311.098 kb on + strand, within Pf6N2E2_249 at 311.098 kb on + strand, within Pf6N2E2_249 at 311.099 kb on - strand, within Pf6N2E2_249
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 308,802 + Pf6N2E2_247 0.18 +0.4 308,802 + Pf6N2E2_247 0.18 +0.1 308,803 - Pf6N2E2_247 0.18 -1.6 309,135 + Pf6N2E2_247 0.50 -0.8 309,135 + Pf6N2E2_247 0.50 -1.7 309,136 - Pf6N2E2_247 0.50 -3.1 309,136 - Pf6N2E2_247 0.50 -0.2 309,367 + Pf6N2E2_247 0.72 -0.0 309,367 + Pf6N2E2_247 0.72 -1.0 309,471 + Pf6N2E2_247 0.82 -0.8 309,474 + Pf6N2E2_247 0.82 +0.5 309,618 - -1.8 309,622 - -1.2 309,726 + -1.3 309,726 + -0.4 309,772 - -2.8 309,772 - -1.5 309,772 - -2.0 309,772 - -0.5 309,772 - -1.2 309,785 + -2.8 309,958 + Pf6N2E2_248 0.44 -3.1 309,958 + Pf6N2E2_248 0.44 -2.8 309,958 + Pf6N2E2_248 0.44 -2.2 309,958 + Pf6N2E2_248 0.44 -2.9 309,958 + Pf6N2E2_248 0.44 -2.3 309,958 + Pf6N2E2_248 0.44 -3.2 309,959 - Pf6N2E2_248 0.44 -2.8 309,959 - Pf6N2E2_248 0.44 -2.2 309,959 - Pf6N2E2_248 0.44 -0.7 309,959 - Pf6N2E2_248 0.44 -1.2 309,959 - Pf6N2E2_248 0.44 -0.8 309,959 - Pf6N2E2_248 0.44 -3.0 309,959 - Pf6N2E2_248 0.44 -3.9 310,013 - Pf6N2E2_248 0.57 -4.2 310,024 + Pf6N2E2_248 0.60 -3.2 310,025 - Pf6N2E2_248 0.60 -3.9 310,025 - Pf6N2E2_248 0.60 -2.2 310,025 - Pf6N2E2_248 0.60 -2.8 310,041 + Pf6N2E2_248 0.64 -4.0 310,042 - Pf6N2E2_248 0.64 -0.8 310,042 - Pf6N2E2_248 0.64 -2.8 310,090 + Pf6N2E2_248 0.76 -3.6 310,090 + Pf6N2E2_248 0.76 -1.6 310,090 + Pf6N2E2_248 0.76 -2.3 310,111 + Pf6N2E2_248 0.81 -3.5 310,111 + Pf6N2E2_248 0.81 -1.2 310,112 - Pf6N2E2_248 0.81 -2.0 310,112 - Pf6N2E2_248 0.81 -2.3 310,117 + Pf6N2E2_248 0.82 -4.0 310,117 + Pf6N2E2_248 0.82 -2.2 310,117 + Pf6N2E2_248 0.82 -4.5 310,117 + Pf6N2E2_248 0.82 -4.5 310,118 - Pf6N2E2_248 0.82 -2.8 310,158 - -2.8 310,176 + -2.8 310,177 - -0.4 310,177 - -2.8 310,177 - -2.2 310,179 - -2.2 310,206 + -2.0 310,207 - +0.4 310,227 + -1.5 310,328 + -2.3 310,393 - +1.2 310,449 + -2.6 310,450 - -0.5 310,450 - +1.1 310,469 + -2.6 310,469 + -4.1 310,469 + -0.9 310,509 + -1.7 310,510 - -0.3 310,517 + -3.3 310,517 + -3.0 310,518 - +1.8 310,526 + -0.8 310,699 - Pf6N2E2_249 0.17 +0.6 310,738 + Pf6N2E2_249 0.23 -1.1 310,738 + Pf6N2E2_249 0.23 -0.7 310,739 - Pf6N2E2_249 0.23 +1.1 310,739 - Pf6N2E2_249 0.23 +0.0 310,739 - Pf6N2E2_249 0.23 +2.2 310,739 - Pf6N2E2_249 0.23 -1.8 310,744 + Pf6N2E2_249 0.23 -3.6 310,745 - Pf6N2E2_249 0.24 -1.0 310,745 - Pf6N2E2_249 0.24 -0.2 310,802 - Pf6N2E2_249 0.31 +1.3 311,098 + Pf6N2E2_249 0.72 -3.2 311,098 + Pf6N2E2_249 0.72 -0.7 311,098 + Pf6N2E2_249 0.72 -3.5 311,099 - Pf6N2E2_249 0.72 -0.3
Or see this region's nucleotide sequence