Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2478 and Pf6N2E2_2479 are separated by 34 nucleotides Pf6N2E2_2479 and Pf6N2E2_2480 are separated by 8 nucleotides Pf6N2E2_2480 and Pf6N2E2_2481 are separated by 299 nucleotides
Pf6N2E2_2478 - FIG00809136: hypothetical protein, at 2,923,643 to 2,926,147
_2478
Pf6N2E2_2479 - Transcription elongation factor GreB, at 2,926,182 to 2,926,679
_2479
Pf6N2E2_2480 - FIG00957636: hypothetical protein, at 2,926,688 to 2,926,945
_2480
Pf6N2E2_2481 - Membrane protein, distant similarity to thiosulphate:quinone oxidoreductase DoxD, at 2,927,245 to 2,927,679
_2481
Position (kb)
2926
2927 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2925.187 kb on + strand, within Pf6N2E2_2478 at 2925.257 kb on + strand, within Pf6N2E2_2478 at 2925.635 kb on + strand, within Pf6N2E2_2478 at 2925.635 kb on + strand, within Pf6N2E2_2478 at 2925.635 kb on + strand, within Pf6N2E2_2478 at 2925.635 kb on + strand, within Pf6N2E2_2478 at 2925.635 kb on + strand, within Pf6N2E2_2478 at 2925.636 kb on - strand, within Pf6N2E2_2478 at 2925.650 kb on + strand, within Pf6N2E2_2478 at 2925.782 kb on + strand, within Pf6N2E2_2478 at 2925.782 kb on + strand, within Pf6N2E2_2478 at 2925.782 kb on + strand, within Pf6N2E2_2478 at 2925.782 kb on + strand, within Pf6N2E2_2478 at 2925.782 kb on + strand, within Pf6N2E2_2478 at 2925.783 kb on - strand, within Pf6N2E2_2478 at 2925.783 kb on - strand, within Pf6N2E2_2478 at 2925.783 kb on - strand, within Pf6N2E2_2478 at 2925.783 kb on - strand, within Pf6N2E2_2478 at 2925.783 kb on - strand, within Pf6N2E2_2478 at 2925.783 kb on - strand, within Pf6N2E2_2478 at 2925.783 kb on - strand, within Pf6N2E2_2478 at 2925.858 kb on + strand at 2925.858 kb on + strand, within Pf6N2E2_2478 at 2925.859 kb on - strand, within Pf6N2E2_2478 at 2925.874 kb on - strand, within Pf6N2E2_2478 at 2926.131 kb on + strand at 2926.131 kb on + strand at 2926.148 kb on + strand at 2926.148 kb on + strand at 2926.148 kb on + strand at 2926.168 kb on + strand at 2926.169 kb on - strand at 2926.169 kb on - strand at 2926.169 kb on - strand at 2926.169 kb on - strand at 2926.350 kb on + strand, within Pf6N2E2_2479 at 2926.350 kb on + strand, within Pf6N2E2_2479 at 2926.350 kb on + strand, within Pf6N2E2_2479 at 2926.350 kb on + strand, within Pf6N2E2_2479 at 2926.357 kb on - strand, within Pf6N2E2_2479 at 2926.464 kb on + strand, within Pf6N2E2_2479 at 2926.465 kb on - strand, within Pf6N2E2_2479 at 2926.653 kb on + strand at 2926.654 kb on - strand at 2926.654 kb on - strand at 2926.672 kb on - strand at 2926.864 kb on + strand, within Pf6N2E2_2480 at 2926.864 kb on + strand, within Pf6N2E2_2480 at 2926.973 kb on + strand at 2926.973 kb on + strand at 2926.973 kb on + strand at 2926.973 kb on + strand at 2926.973 kb on + strand at 2926.973 kb on + strand at 2926.973 kb on + strand at 2926.973 kb on + strand at 2926.974 kb on - strand at 2926.974 kb on - strand at 2926.974 kb on - strand at 2926.974 kb on - strand at 2927.107 kb on + strand at 2927.107 kb on + strand at 2927.130 kb on + strand at 2927.130 kb on + strand at 2927.130 kb on + strand at 2927.131 kb on - strand at 2927.131 kb on - strand at 2927.162 kb on + strand at 2927.162 kb on + strand at 2927.214 kb on - strand at 2927.305 kb on - strand, within Pf6N2E2_2481 at 2927.337 kb on - strand, within Pf6N2E2_2481 at 2927.599 kb on + strand, within Pf6N2E2_2481 at 2927.599 kb on + strand, within Pf6N2E2_2481 at 2927.599 kb on + strand, within Pf6N2E2_2481 at 2927.600 kb on - strand, within Pf6N2E2_2481 at 2927.600 kb on - strand, within Pf6N2E2_2481 at 2927.661 kb on + strand at 2927.662 kb on - strand at 2927.662 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,925,187 + Pf6N2E2_2478 0.62 +2.0 2,925,257 + Pf6N2E2_2478 0.64 +0.5 2,925,635 + Pf6N2E2_2478 0.80 +0.9 2,925,635 + Pf6N2E2_2478 0.80 -1.3 2,925,635 + Pf6N2E2_2478 0.80 -0.7 2,925,635 + Pf6N2E2_2478 0.80 -1.5 2,925,635 + Pf6N2E2_2478 0.80 +0.6 2,925,636 - Pf6N2E2_2478 0.80 -0.3 2,925,650 + Pf6N2E2_2478 0.80 +0.0 2,925,782 + Pf6N2E2_2478 0.85 -0.4 2,925,782 + Pf6N2E2_2478 0.85 -1.2 2,925,782 + Pf6N2E2_2478 0.85 -0.2 2,925,782 + Pf6N2E2_2478 0.85 -0.7 2,925,782 + Pf6N2E2_2478 0.85 -0.8 2,925,783 - Pf6N2E2_2478 0.85 -1.0 2,925,783 - Pf6N2E2_2478 0.85 +0.5 2,925,783 - Pf6N2E2_2478 0.85 -0.2 2,925,783 - Pf6N2E2_2478 0.85 -1.2 2,925,783 - Pf6N2E2_2478 0.85 -1.4 2,925,783 - Pf6N2E2_2478 0.85 -1.3 2,925,783 - Pf6N2E2_2478 0.85 -0.6 2,925,858 + -0.2 2,925,858 + Pf6N2E2_2478 0.88 -0.2 2,925,859 - Pf6N2E2_2478 0.88 -1.2 2,925,874 - Pf6N2E2_2478 0.89 -0.4 2,926,131 + -0.2 2,926,131 + +1.6 2,926,148 + +0.2 2,926,148 + +0.5 2,926,148 + -0.6 2,926,168 + -2.5 2,926,169 - -1.1 2,926,169 - +0.5 2,926,169 - +1.3 2,926,169 - -1.7 2,926,350 + Pf6N2E2_2479 0.34 -0.4 2,926,350 + Pf6N2E2_2479 0.34 -1.3 2,926,350 + Pf6N2E2_2479 0.34 -3.9 2,926,350 + Pf6N2E2_2479 0.34 -1.5 2,926,357 - Pf6N2E2_2479 0.35 +0.0 2,926,464 + Pf6N2E2_2479 0.57 -1.7 2,926,465 - Pf6N2E2_2479 0.57 -1.4 2,926,653 + -0.9 2,926,654 - +0.4 2,926,654 - +0.8 2,926,672 - +1.3 2,926,864 + Pf6N2E2_2480 0.68 +1.4 2,926,864 + Pf6N2E2_2480 0.68 -2.0 2,926,973 + -0.4 2,926,973 + +0.6 2,926,973 + -0.0 2,926,973 + -0.6 2,926,973 + -0.4 2,926,973 + -0.9 2,926,973 + -1.3 2,926,973 + -0.7 2,926,974 - -0.3 2,926,974 - +0.1 2,926,974 - -0.2 2,926,974 - -4.0 2,927,107 + +0.3 2,927,107 + -0.2 2,927,130 + -0.2 2,927,130 + -0.2 2,927,130 + +0.1 2,927,131 - -1.7 2,927,131 - -1.7 2,927,162 + +0.0 2,927,162 + -0.4 2,927,214 - -0.1 2,927,305 - Pf6N2E2_2481 0.14 -1.0 2,927,337 - Pf6N2E2_2481 0.21 -0.4 2,927,599 + Pf6N2E2_2481 0.81 -1.7 2,927,599 + Pf6N2E2_2481 0.81 -0.3 2,927,599 + Pf6N2E2_2481 0.81 -0.6 2,927,600 - Pf6N2E2_2481 0.82 +1.2 2,927,600 - Pf6N2E2_2481 0.82 +0.1 2,927,661 + +0.1 2,927,662 - -1.0 2,927,662 - +0.2
Or see this region's nucleotide sequence