Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2474 and Pf6N2E2_2475 are separated by 166 nucleotides Pf6N2E2_2475 and Pf6N2E2_2476 are separated by 56 nucleotides Pf6N2E2_2476 and Pf6N2E2_2477 are separated by 10 nucleotides Pf6N2E2_2477 and Pf6N2E2_2478 overlap by 1 nucleotides
Pf6N2E2_2474 - ErfK/YbiS/YcfS/YnhG family protein, at 2,920,856 to 2,921,827
_2474
Pf6N2E2_2475 - FIG00953861: hypothetical protein, at 2,921,994 to 2,922,287
_2475
Pf6N2E2_2476 - Arylesterase precursor (EC 3.1.1.2), at 2,922,344 to 2,922,949
_2476
Pf6N2E2_2477 - ABC transporter ATP-binding protein YvcR, at 2,922,960 to 2,923,643
_2477
Pf6N2E2_2478 - FIG00809136: hypothetical protein, at 2,923,643 to 2,926,147
_2478
Position (kb)
2922
2923 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2921.469 kb on + strand, within Pf6N2E2_2474 at 2921.469 kb on + strand, within Pf6N2E2_2474 at 2921.470 kb on - strand, within Pf6N2E2_2474 at 2921.470 kb on - strand, within Pf6N2E2_2474 at 2921.497 kb on - strand, within Pf6N2E2_2474 at 2921.497 kb on - strand, within Pf6N2E2_2474 at 2921.497 kb on - strand, within Pf6N2E2_2474 at 2921.499 kb on + strand, within Pf6N2E2_2474 at 2921.500 kb on - strand, within Pf6N2E2_2474 at 2921.640 kb on + strand, within Pf6N2E2_2474 at 2921.652 kb on + strand, within Pf6N2E2_2474 at 2921.652 kb on + strand, within Pf6N2E2_2474 at 2921.653 kb on - strand, within Pf6N2E2_2474 at 2921.653 kb on - strand, within Pf6N2E2_2474 at 2921.653 kb on - strand, within Pf6N2E2_2474 at 2921.653 kb on - strand, within Pf6N2E2_2474 at 2921.653 kb on - strand, within Pf6N2E2_2474 at 2921.653 kb on - strand, within Pf6N2E2_2474 at 2921.685 kb on + strand, within Pf6N2E2_2474 at 2921.685 kb on + strand, within Pf6N2E2_2474 at 2921.685 kb on + strand, within Pf6N2E2_2474 at 2921.773 kb on + strand at 2921.877 kb on + strand at 2921.877 kb on + strand at 2921.878 kb on - strand at 2921.878 kb on - strand at 2921.878 kb on - strand at 2921.878 kb on - strand at 2921.878 kb on - strand at 2921.892 kb on + strand at 2921.893 kb on - strand at 2921.996 kb on - strand at 2921.996 kb on - strand at 2921.996 kb on - strand at 2922.372 kb on - strand at 2922.372 kb on - strand at 2922.372 kb on - strand at 2922.507 kb on + strand, within Pf6N2E2_2476 at 2922.508 kb on - strand, within Pf6N2E2_2476 at 2922.532 kb on - strand, within Pf6N2E2_2476 at 2922.543 kb on + strand, within Pf6N2E2_2476 at 2922.775 kb on + strand, within Pf6N2E2_2476 at 2922.859 kb on + strand, within Pf6N2E2_2476 at 2922.860 kb on - strand, within Pf6N2E2_2476 at 2922.884 kb on - strand, within Pf6N2E2_2476 at 2922.992 kb on + strand at 2923.181 kb on + strand, within Pf6N2E2_2477 at 2923.181 kb on + strand, within Pf6N2E2_2477 at 2923.597 kb on + strand at 2923.762 kb on + strand at 2923.776 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,921,469 + Pf6N2E2_2474 0.63 -3.2 2,921,469 + Pf6N2E2_2474 0.63 -2.3 2,921,470 - Pf6N2E2_2474 0.63 -1.1 2,921,470 - Pf6N2E2_2474 0.63 -4.2 2,921,497 - Pf6N2E2_2474 0.66 -1.5 2,921,497 - Pf6N2E2_2474 0.66 -2.3 2,921,497 - Pf6N2E2_2474 0.66 +0.6 2,921,499 + Pf6N2E2_2474 0.66 -1.5 2,921,500 - Pf6N2E2_2474 0.66 -1.5 2,921,640 + Pf6N2E2_2474 0.81 -0.6 2,921,652 + Pf6N2E2_2474 0.82 -1.2 2,921,652 + Pf6N2E2_2474 0.82 -2.7 2,921,653 - Pf6N2E2_2474 0.82 -2.0 2,921,653 - Pf6N2E2_2474 0.82 -2.0 2,921,653 - Pf6N2E2_2474 0.82 -1.3 2,921,653 - Pf6N2E2_2474 0.82 -0.9 2,921,653 - Pf6N2E2_2474 0.82 -1.6 2,921,653 - Pf6N2E2_2474 0.82 -1.2 2,921,685 + Pf6N2E2_2474 0.85 -1.1 2,921,685 + Pf6N2E2_2474 0.85 -2.7 2,921,685 + Pf6N2E2_2474 0.85 -1.6 2,921,773 + -2.5 2,921,877 + -1.9 2,921,877 + -0.5 2,921,878 - -0.4 2,921,878 - +0.2 2,921,878 - -0.6 2,921,878 - -0.8 2,921,878 - +0.6 2,921,892 + -1.2 2,921,893 - -1.0 2,921,996 - -1.0 2,921,996 - -0.5 2,921,996 - +0.1 2,922,372 - +1.1 2,922,372 - +0.6 2,922,372 - -1.7 2,922,507 + Pf6N2E2_2476 0.27 -1.7 2,922,508 - Pf6N2E2_2476 0.27 -1.3 2,922,532 - Pf6N2E2_2476 0.31 -1.7 2,922,543 + Pf6N2E2_2476 0.33 -2.0 2,922,775 + Pf6N2E2_2476 0.71 -0.2 2,922,859 + Pf6N2E2_2476 0.85 -0.7 2,922,860 - Pf6N2E2_2476 0.85 -0.2 2,922,884 - Pf6N2E2_2476 0.89 +0.4 2,922,992 + +0.1 2,923,181 + Pf6N2E2_2477 0.32 +0.1 2,923,181 + Pf6N2E2_2477 0.32 -0.2 2,923,597 + -1.3 2,923,762 + -0.1 2,923,776 - +0.2
Or see this region's nucleotide sequence