Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2467 and Pf6N2E2_2468 are separated by 20 nucleotides Pf6N2E2_2468 and Pf6N2E2_2469 are separated by 51 nucleotides Pf6N2E2_2469 and Pf6N2E2_2470 are separated by 16 nucleotides
Pf6N2E2_2467 - 5'-nucleotidase (EC 3.1.3.5), at 2,916,158 to 2,917,063
_2467
Pf6N2E2_2468 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 2,917,084 to 2,918,037
_2468
Pf6N2E2_2469 - hypothetical protein, at 2,918,089 to 2,918,289
_2469
Pf6N2E2_2470 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 2,918,306 to 2,919,382
_2470
Position (kb)
2917
2918
2919 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2916.104 kb on + strand at 2916.104 kb on + strand at 2916.104 kb on + strand at 2916.104 kb on + strand at 2916.104 kb on + strand at 2916.105 kb on - strand at 2916.105 kb on - strand at 2916.105 kb on - strand at 2916.328 kb on + strand, within Pf6N2E2_2467 at 2916.329 kb on - strand, within Pf6N2E2_2467 at 2916.398 kb on - strand, within Pf6N2E2_2467 at 2916.541 kb on - strand, within Pf6N2E2_2467 at 2916.726 kb on + strand, within Pf6N2E2_2467 at 2916.981 kb on + strand at 2916.981 kb on + strand at 2916.981 kb on + strand at 2916.981 kb on + strand at 2916.982 kb on - strand at 2916.982 kb on - strand at 2917.092 kb on + strand at 2917.092 kb on + strand at 2917.093 kb on - strand at 2917.093 kb on - strand at 2917.095 kb on - strand at 2917.095 kb on - strand at 2917.095 kb on - strand at 2917.121 kb on + strand at 2917.122 kb on - strand at 2917.122 kb on - strand at 2917.122 kb on - strand at 2917.122 kb on - strand at 2917.184 kb on + strand, within Pf6N2E2_2468 at 2917.185 kb on - strand, within Pf6N2E2_2468 at 2917.220 kb on + strand, within Pf6N2E2_2468 at 2917.221 kb on - strand, within Pf6N2E2_2468 at 2917.221 kb on - strand at 2917.229 kb on + strand, within Pf6N2E2_2468 at 2917.229 kb on + strand, within Pf6N2E2_2468 at 2917.229 kb on + strand, within Pf6N2E2_2468 at 2917.229 kb on + strand, within Pf6N2E2_2468 at 2917.230 kb on - strand, within Pf6N2E2_2468 at 2917.245 kb on - strand, within Pf6N2E2_2468 at 2917.277 kb on + strand, within Pf6N2E2_2468 at 2917.278 kb on - strand, within Pf6N2E2_2468 at 2917.300 kb on - strand, within Pf6N2E2_2468 at 2917.365 kb on + strand, within Pf6N2E2_2468 at 2917.392 kb on + strand, within Pf6N2E2_2468 at 2917.425 kb on - strand, within Pf6N2E2_2468 at 2917.425 kb on - strand, within Pf6N2E2_2468 at 2917.425 kb on - strand, within Pf6N2E2_2468 at 2918.056 kb on - strand at 2918.073 kb on + strand at 2918.073 kb on + strand at 2918.073 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.107 kb on + strand at 2918.108 kb on - strand at 2918.108 kb on - strand at 2918.161 kb on + strand, within Pf6N2E2_2469 at 2918.162 kb on - strand, within Pf6N2E2_2469 at 2918.162 kb on - strand, within Pf6N2E2_2469 at 2918.163 kb on + strand, within Pf6N2E2_2469 at 2918.172 kb on - strand, within Pf6N2E2_2469 at 2918.238 kb on - strand, within Pf6N2E2_2469 at 2918.238 kb on - strand, within Pf6N2E2_2469 at 2918.300 kb on - strand at 2918.333 kb on - strand at 2918.523 kb on - strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.571 kb on - strand, within Pf6N2E2_2470 at 2918.571 kb on - strand, within Pf6N2E2_2470 at 2918.588 kb on + strand, within Pf6N2E2_2470 at 2918.588 kb on + strand, within Pf6N2E2_2470 at 2918.588 kb on + strand, within Pf6N2E2_2470 at 2918.589 kb on - strand, within Pf6N2E2_2470 at 2918.642 kb on + strand, within Pf6N2E2_2470 at 2918.642 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.990 kb on + strand, within Pf6N2E2_2470 at 2918.991 kb on - strand, within Pf6N2E2_2470 at 2919.032 kb on + strand, within Pf6N2E2_2470 at 2919.033 kb on - strand, within Pf6N2E2_2470 at 2919.033 kb on - strand, within Pf6N2E2_2470
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,916,104 + -2.2 2,916,104 + -1.7 2,916,104 + -0.7 2,916,104 + -0.0 2,916,104 + -0.4 2,916,105 - +2.2 2,916,105 - +0.0 2,916,105 - +0.2 2,916,328 + Pf6N2E2_2467 0.19 -2.2 2,916,329 - Pf6N2E2_2467 0.19 -1.3 2,916,398 - Pf6N2E2_2467 0.26 -2.4 2,916,541 - Pf6N2E2_2467 0.42 -0.8 2,916,726 + Pf6N2E2_2467 0.63 -0.3 2,916,981 + -0.3 2,916,981 + -0.4 2,916,981 + +0.1 2,916,981 + -1.5 2,916,982 - +0.2 2,916,982 - +0.8 2,917,092 + +0.3 2,917,092 + -2.5 2,917,093 - +1.8 2,917,093 - -0.6 2,917,095 - +2.5 2,917,095 - +1.4 2,917,095 - -0.5 2,917,121 + -1.2 2,917,122 - -1.3 2,917,122 - +1.1 2,917,122 - +0.3 2,917,122 - +0.8 2,917,184 + Pf6N2E2_2468 0.10 -2.5 2,917,185 - Pf6N2E2_2468 0.11 +1.0 2,917,220 + Pf6N2E2_2468 0.14 -0.5 2,917,221 - Pf6N2E2_2468 0.14 -1.2 2,917,221 - +0.8 2,917,229 + Pf6N2E2_2468 0.15 -0.9 2,917,229 + Pf6N2E2_2468 0.15 +0.6 2,917,229 + Pf6N2E2_2468 0.15 -1.7 2,917,229 + Pf6N2E2_2468 0.15 -2.7 2,917,230 - Pf6N2E2_2468 0.15 -0.3 2,917,245 - Pf6N2E2_2468 0.17 +0.6 2,917,277 + Pf6N2E2_2468 0.20 -1.0 2,917,278 - Pf6N2E2_2468 0.20 -1.3 2,917,300 - Pf6N2E2_2468 0.23 +0.3 2,917,365 + Pf6N2E2_2468 0.29 -0.7 2,917,392 + Pf6N2E2_2468 0.32 +1.7 2,917,425 - Pf6N2E2_2468 0.36 -1.6 2,917,425 - Pf6N2E2_2468 0.36 -0.5 2,917,425 - Pf6N2E2_2468 0.36 -0.4 2,918,056 - +0.7 2,918,073 + -1.2 2,918,073 + -0.4 2,918,073 + -3.5 2,918,107 + -1.9 2,918,107 + -1.1 2,918,107 + -0.6 2,918,107 + -0.2 2,918,107 + -0.2 2,918,107 + -0.7 2,918,107 + +1.1 2,918,107 + -1.5 2,918,108 - -0.7 2,918,108 - -1.9 2,918,161 + Pf6N2E2_2469 0.36 -0.7 2,918,162 - Pf6N2E2_2469 0.36 -1.3 2,918,162 - Pf6N2E2_2469 0.36 -2.9 2,918,163 + Pf6N2E2_2469 0.37 -0.2 2,918,172 - Pf6N2E2_2469 0.41 -2.1 2,918,238 - Pf6N2E2_2469 0.74 -2.3 2,918,238 - Pf6N2E2_2469 0.74 -1.5 2,918,300 - -0.7 2,918,333 - -1.7 2,918,523 - Pf6N2E2_2470 0.20 -0.5 2,918,570 + Pf6N2E2_2470 0.25 -1.6 2,918,570 + Pf6N2E2_2470 0.25 -1.2 2,918,570 + Pf6N2E2_2470 0.25 -1.4 2,918,570 + Pf6N2E2_2470 0.25 -1.5 2,918,571 - Pf6N2E2_2470 0.25 -4.2 2,918,571 - Pf6N2E2_2470 0.25 -2.4 2,918,588 + Pf6N2E2_2470 0.26 -2.3 2,918,588 + Pf6N2E2_2470 0.26 -1.5 2,918,588 + Pf6N2E2_2470 0.26 -2.7 2,918,589 - Pf6N2E2_2470 0.26 -2.2 2,918,642 + Pf6N2E2_2470 0.31 -0.9 2,918,642 + Pf6N2E2_2470 0.31 -1.4 2,918,762 + Pf6N2E2_2470 0.42 -1.4 2,918,762 + Pf6N2E2_2470 0.42 -1.2 2,918,762 + Pf6N2E2_2470 0.42 -1.7 2,918,762 + Pf6N2E2_2470 0.42 -0.5 2,918,763 - Pf6N2E2_2470 0.42 -2.5 2,918,763 - Pf6N2E2_2470 0.42 -1.7 2,918,763 - Pf6N2E2_2470 0.42 -2.2 2,918,763 - Pf6N2E2_2470 0.42 -2.0 2,918,990 + Pf6N2E2_2470 0.64 -1.7 2,918,991 - Pf6N2E2_2470 0.64 -2.2 2,919,032 + Pf6N2E2_2470 0.67 -1.7 2,919,033 - Pf6N2E2_2470 0.68 -2.5 2,919,033 - Pf6N2E2_2470 0.68 -1.6
Or see this region's nucleotide sequence