Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2452 and Pf6N2E2_2453 are separated by 139 nucleotides Pf6N2E2_2453 and Pf6N2E2_2454 are separated by 128 nucleotides
Pf6N2E2_2452 - Spermidine synthase (EC 2.5.1.16), at 2,903,504 to 2,904,259
_2452
Pf6N2E2_2453 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase II (EC 2.5.1.54), at 2,904,399 to 2,905,748
_2453
Pf6N2E2_2454 - FIG074102: hypothetical protein, at 2,905,877 to 2,907,106
_2454
Position (kb)
2904
2905
2906 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2903.482 kb on - strand at 2903.679 kb on + strand, within Pf6N2E2_2452 at 2903.680 kb on - strand, within Pf6N2E2_2452 at 2903.680 kb on - strand, within Pf6N2E2_2452 at 2903.827 kb on + strand, within Pf6N2E2_2452 at 2903.830 kb on - strand, within Pf6N2E2_2452 at 2903.901 kb on + strand, within Pf6N2E2_2452 at 2903.901 kb on + strand, within Pf6N2E2_2452 at 2903.901 kb on + strand, within Pf6N2E2_2452 at 2903.902 kb on - strand, within Pf6N2E2_2452 at 2903.902 kb on - strand, within Pf6N2E2_2452 at 2903.902 kb on - strand, within Pf6N2E2_2452 at 2903.931 kb on + strand, within Pf6N2E2_2452 at 2904.022 kb on + strand, within Pf6N2E2_2452 at 2904.023 kb on - strand, within Pf6N2E2_2452 at 2904.023 kb on - strand, within Pf6N2E2_2452 at 2904.023 kb on - strand, within Pf6N2E2_2452 at 2904.097 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.106 kb on - strand, within Pf6N2E2_2452 at 2904.352 kb on + strand at 2904.353 kb on - strand at 2904.363 kb on + strand at 2904.391 kb on + strand at 2904.392 kb on - strand at 2904.874 kb on - strand, within Pf6N2E2_2453 at 2904.874 kb on - strand, within Pf6N2E2_2453 at 2904.996 kb on + strand, within Pf6N2E2_2453 at 2905.119 kb on + strand, within Pf6N2E2_2453 at 2905.120 kb on - strand, within Pf6N2E2_2453 at 2905.162 kb on - strand, within Pf6N2E2_2453 at 2905.164 kb on + strand, within Pf6N2E2_2453 at 2905.164 kb on + strand, within Pf6N2E2_2453 at 2905.164 kb on + strand, within Pf6N2E2_2453 at 2905.165 kb on - strand, within Pf6N2E2_2453 at 2905.300 kb on + strand, within Pf6N2E2_2453 at 2905.300 kb on + strand, within Pf6N2E2_2453 at 2905.301 kb on - strand, within Pf6N2E2_2453 at 2905.301 kb on - strand, within Pf6N2E2_2453 at 2905.301 kb on - strand, within Pf6N2E2_2453 at 2905.301 kb on - strand, within Pf6N2E2_2453 at 2905.485 kb on + strand, within Pf6N2E2_2453 at 2905.560 kb on + strand, within Pf6N2E2_2453 at 2905.560 kb on + strand, within Pf6N2E2_2453 at 2905.561 kb on - strand, within Pf6N2E2_2453 at 2905.561 kb on - strand, within Pf6N2E2_2453 at 2905.561 kb on - strand, within Pf6N2E2_2453 at 2905.889 kb on + strand at 2905.889 kb on + strand at 2905.890 kb on - strand at 2905.890 kb on - strand at 2905.890 kb on - strand at 2905.940 kb on - strand at 2905.940 kb on - strand at 2905.940 kb on - strand at 2906.130 kb on + strand, within Pf6N2E2_2454 at 2906.130 kb on + strand, within Pf6N2E2_2454 at 2906.130 kb on + strand, within Pf6N2E2_2454 at 2906.131 kb on - strand, within Pf6N2E2_2454 at 2906.131 kb on - strand, within Pf6N2E2_2454 at 2906.131 kb on - strand, within Pf6N2E2_2454 at 2906.133 kb on + strand, within Pf6N2E2_2454 at 2906.134 kb on - strand, within Pf6N2E2_2454 at 2906.134 kb on - strand, within Pf6N2E2_2454 at 2906.139 kb on + strand, within Pf6N2E2_2454 at 2906.139 kb on + strand, within Pf6N2E2_2454 at 2906.139 kb on + strand, within Pf6N2E2_2454 at 2906.139 kb on + strand, within Pf6N2E2_2454 at 2906.139 kb on + strand, within Pf6N2E2_2454 at 2906.139 kb on + strand, within Pf6N2E2_2454 at 2906.140 kb on - strand, within Pf6N2E2_2454 at 2906.140 kb on - strand, within Pf6N2E2_2454 at 2906.140 kb on - strand, within Pf6N2E2_2454 at 2906.140 kb on - strand, within Pf6N2E2_2454 at 2906.140 kb on - strand, within Pf6N2E2_2454 at 2906.163 kb on + strand, within Pf6N2E2_2454 at 2906.163 kb on + strand, within Pf6N2E2_2454 at 2906.163 kb on + strand, within Pf6N2E2_2454 at 2906.163 kb on + strand, within Pf6N2E2_2454 at 2906.163 kb on + strand, within Pf6N2E2_2454 at 2906.163 kb on + strand, within Pf6N2E2_2454 at 2906.163 kb on + strand, within Pf6N2E2_2454 at 2906.163 kb on + strand, within Pf6N2E2_2454 at 2906.163 kb on + strand, within Pf6N2E2_2454 at 2906.163 kb on + strand, within Pf6N2E2_2454 at 2906.163 kb on + strand, within Pf6N2E2_2454 at 2906.164 kb on - strand, within Pf6N2E2_2454 at 2906.164 kb on - strand, within Pf6N2E2_2454 at 2906.164 kb on - strand, within Pf6N2E2_2454 at 2906.164 kb on - strand, within Pf6N2E2_2454 at 2906.164 kb on - strand, within Pf6N2E2_2454 at 2906.164 kb on - strand, within Pf6N2E2_2454 at 2906.164 kb on - strand, within Pf6N2E2_2454 at 2906.394 kb on + strand, within Pf6N2E2_2454 at 2906.510 kb on + strand, within Pf6N2E2_2454 at 2906.529 kb on + strand, within Pf6N2E2_2454 at 2906.530 kb on - strand, within Pf6N2E2_2454 at 2906.530 kb on - strand, within Pf6N2E2_2454 at 2906.530 kb on - strand, within Pf6N2E2_2454 at 2906.530 kb on - strand, within Pf6N2E2_2454 at 2906.647 kb on + strand, within Pf6N2E2_2454 at 2906.648 kb on - strand, within Pf6N2E2_2454 at 2906.748 kb on + strand, within Pf6N2E2_2454 at 2906.748 kb on + strand, within Pf6N2E2_2454 at 2906.748 kb on + strand, within Pf6N2E2_2454
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,903,482 - +2.5 2,903,679 + Pf6N2E2_2452 0.23 -0.4 2,903,680 - Pf6N2E2_2452 0.23 -0.9 2,903,680 - Pf6N2E2_2452 0.23 +1.2 2,903,827 + Pf6N2E2_2452 0.43 -0.2 2,903,830 - Pf6N2E2_2452 0.43 +0.3 2,903,901 + Pf6N2E2_2452 0.53 -0.2 2,903,901 + Pf6N2E2_2452 0.53 -0.6 2,903,901 + Pf6N2E2_2452 0.53 -0.7 2,903,902 - Pf6N2E2_2452 0.53 -1.0 2,903,902 - Pf6N2E2_2452 0.53 -0.5 2,903,902 - Pf6N2E2_2452 0.53 -0.1 2,903,931 + Pf6N2E2_2452 0.56 +0.2 2,904,022 + Pf6N2E2_2452 0.69 +0.3 2,904,023 - Pf6N2E2_2452 0.69 +0.3 2,904,023 - Pf6N2E2_2452 0.69 -0.4 2,904,023 - Pf6N2E2_2452 0.69 -1.2 2,904,097 + Pf6N2E2_2452 0.78 -2.8 2,904,099 + Pf6N2E2_2452 0.79 -0.9 2,904,099 + Pf6N2E2_2452 0.79 +2.6 2,904,099 + Pf6N2E2_2452 0.79 -0.7 2,904,099 + Pf6N2E2_2452 0.79 +0.5 2,904,099 + Pf6N2E2_2452 0.79 +0.4 2,904,099 + Pf6N2E2_2452 0.79 -2.5 2,904,100 - Pf6N2E2_2452 0.79 +0.7 2,904,100 - Pf6N2E2_2452 0.79 +1.1 2,904,100 - Pf6N2E2_2452 0.79 -0.2 2,904,100 - Pf6N2E2_2452 0.79 +1.9 2,904,100 - Pf6N2E2_2452 0.79 -0.5 2,904,106 - Pf6N2E2_2452 0.80 +1.6 2,904,352 + +0.0 2,904,353 - -0.8 2,904,363 + +0.6 2,904,391 + +0.7 2,904,392 - +1.4 2,904,874 - Pf6N2E2_2453 0.35 -0.4 2,904,874 - Pf6N2E2_2453 0.35 -0.6 2,904,996 + Pf6N2E2_2453 0.44 -0.3 2,905,119 + Pf6N2E2_2453 0.53 -0.2 2,905,120 - Pf6N2E2_2453 0.53 -0.1 2,905,162 - Pf6N2E2_2453 0.57 +1.2 2,905,164 + Pf6N2E2_2453 0.57 +0.4 2,905,164 + Pf6N2E2_2453 0.57 -1.5 2,905,164 + Pf6N2E2_2453 0.57 -1.2 2,905,165 - Pf6N2E2_2453 0.57 +2.1 2,905,300 + Pf6N2E2_2453 0.67 +0.2 2,905,300 + Pf6N2E2_2453 0.67 -0.4 2,905,301 - Pf6N2E2_2453 0.67 +1.1 2,905,301 - Pf6N2E2_2453 0.67 +0.7 2,905,301 - Pf6N2E2_2453 0.67 +0.5 2,905,301 - Pf6N2E2_2453 0.67 -1.0 2,905,485 + Pf6N2E2_2453 0.80 +0.4 2,905,560 + Pf6N2E2_2453 0.86 +0.6 2,905,560 + Pf6N2E2_2453 0.86 +0.0 2,905,561 - Pf6N2E2_2453 0.86 +0.7 2,905,561 - Pf6N2E2_2453 0.86 -1.0 2,905,561 - Pf6N2E2_2453 0.86 +0.4 2,905,889 + -0.3 2,905,889 + -0.7 2,905,890 - +1.2 2,905,890 - -0.5 2,905,890 - +0.9 2,905,940 - +0.8 2,905,940 - +0.2 2,905,940 - -1.0 2,906,130 + Pf6N2E2_2454 0.21 +0.9 2,906,130 + Pf6N2E2_2454 0.21 +1.0 2,906,130 + Pf6N2E2_2454 0.21 +0.6 2,906,131 - Pf6N2E2_2454 0.21 -0.4 2,906,131 - Pf6N2E2_2454 0.21 +0.0 2,906,131 - Pf6N2E2_2454 0.21 +0.4 2,906,133 + Pf6N2E2_2454 0.21 -0.4 2,906,134 - Pf6N2E2_2454 0.21 -0.1 2,906,134 - Pf6N2E2_2454 0.21 +0.6 2,906,139 + Pf6N2E2_2454 0.21 +0.3 2,906,139 + Pf6N2E2_2454 0.21 +0.7 2,906,139 + Pf6N2E2_2454 0.21 -0.6 2,906,139 + Pf6N2E2_2454 0.21 -0.3 2,906,139 + Pf6N2E2_2454 0.21 -0.9 2,906,139 + Pf6N2E2_2454 0.21 -1.4 2,906,140 - Pf6N2E2_2454 0.21 +0.8 2,906,140 - Pf6N2E2_2454 0.21 +1.0 2,906,140 - Pf6N2E2_2454 0.21 -0.8 2,906,140 - Pf6N2E2_2454 0.21 +1.2 2,906,140 - Pf6N2E2_2454 0.21 +0.2 2,906,163 + Pf6N2E2_2454 0.23 +1.8 2,906,163 + Pf6N2E2_2454 0.23 +0.8 2,906,163 + Pf6N2E2_2454 0.23 +0.5 2,906,163 + Pf6N2E2_2454 0.23 -0.6 2,906,163 + Pf6N2E2_2454 0.23 +0.9 2,906,163 + Pf6N2E2_2454 0.23 +1.8 2,906,163 + Pf6N2E2_2454 0.23 +0.1 2,906,163 + Pf6N2E2_2454 0.23 -1.0 2,906,163 + Pf6N2E2_2454 0.23 -2.8 2,906,163 + Pf6N2E2_2454 0.23 +0.2 2,906,163 + Pf6N2E2_2454 0.23 +0.9 2,906,164 - Pf6N2E2_2454 0.23 +0.7 2,906,164 - Pf6N2E2_2454 0.23 -1.2 2,906,164 - Pf6N2E2_2454 0.23 +0.3 2,906,164 - Pf6N2E2_2454 0.23 +1.4 2,906,164 - Pf6N2E2_2454 0.23 -0.8 2,906,164 - Pf6N2E2_2454 0.23 +0.5 2,906,164 - Pf6N2E2_2454 0.23 +0.8 2,906,394 + Pf6N2E2_2454 0.42 -0.9 2,906,510 + Pf6N2E2_2454 0.51 -1.4 2,906,529 + Pf6N2E2_2454 0.53 +1.2 2,906,530 - Pf6N2E2_2454 0.53 +0.1 2,906,530 - Pf6N2E2_2454 0.53 -0.2 2,906,530 - Pf6N2E2_2454 0.53 -0.2 2,906,530 - Pf6N2E2_2454 0.53 +0.4 2,906,647 + Pf6N2E2_2454 0.63 -1.3 2,906,648 - Pf6N2E2_2454 0.63 -1.2 2,906,748 + Pf6N2E2_2454 0.71 -1.2 2,906,748 + Pf6N2E2_2454 0.71 +1.0 2,906,748 + Pf6N2E2_2454 0.71 -0.2
Or see this region's nucleotide sequence