Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2384 and Pf6N2E2_2385 are separated by 2 nucleotides Pf6N2E2_2385 and Pf6N2E2_2386 are separated by 63 nucleotides Pf6N2E2_2386 and Pf6N2E2_2387 are separated by 25 nucleotides Pf6N2E2_2387 and Pf6N2E2_2388 are separated by 126 nucleotides
Pf6N2E2_2384 - CmpX, at 2,823,206 to 2,824,030
_2384
Pf6N2E2_2385 - CrfX protein, at 2,824,033 to 2,824,278
_2385
Pf6N2E2_2386 - Magnesium and cobalt transport protein CorA, at 2,824,342 to 2,825,337
_2386
Pf6N2E2_2387 - Ribonuclease E inhibitor RraA, at 2,825,363 to 2,825,854
_2387
Pf6N2E2_2388 - Esterase/lipase/thioesterase family protein, at 2,825,981 to 2,826,970
_2388
Position (kb)
2824
2825
2826 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2823.355 kb on - strand, within Pf6N2E2_2384 at 2823.370 kb on - strand, within Pf6N2E2_2384 at 2823.370 kb on - strand, within Pf6N2E2_2384 at 2823.370 kb on - strand, within Pf6N2E2_2384 at 2823.518 kb on - strand, within Pf6N2E2_2384 at 2823.518 kb on - strand, within Pf6N2E2_2384 at 2823.518 kb on - strand, within Pf6N2E2_2384 at 2823.689 kb on - strand, within Pf6N2E2_2384 at 2823.756 kb on - strand, within Pf6N2E2_2384 at 2823.756 kb on - strand, within Pf6N2E2_2384 at 2823.756 kb on - strand, within Pf6N2E2_2384 at 2823.965 kb on - strand at 2823.965 kb on - strand at 2823.969 kb on - strand at 2824.030 kb on + strand at 2824.031 kb on - strand at 2824.045 kb on - strand at 2824.060 kb on - strand, within Pf6N2E2_2385 at 2824.523 kb on + strand, within Pf6N2E2_2386 at 2824.523 kb on + strand, within Pf6N2E2_2386 at 2824.524 kb on - strand, within Pf6N2E2_2386 at 2824.650 kb on - strand, within Pf6N2E2_2386 at 2824.787 kb on + strand, within Pf6N2E2_2386 at 2824.787 kb on + strand, within Pf6N2E2_2386 at 2824.788 kb on - strand, within Pf6N2E2_2386 at 2824.788 kb on - strand, within Pf6N2E2_2386 at 2824.788 kb on - strand, within Pf6N2E2_2386 at 2824.886 kb on + strand, within Pf6N2E2_2386 at 2824.887 kb on - strand, within Pf6N2E2_2386 at 2825.356 kb on + strand at 2825.356 kb on + strand at 2825.357 kb on - strand at 2825.419 kb on - strand, within Pf6N2E2_2387 at 2825.419 kb on - strand, within Pf6N2E2_2387 at 2825.419 kb on - strand, within Pf6N2E2_2387 at 2825.419 kb on - strand, within Pf6N2E2_2387 at 2825.424 kb on + strand, within Pf6N2E2_2387 at 2825.424 kb on + strand, within Pf6N2E2_2387 at 2825.424 kb on + strand, within Pf6N2E2_2387 at 2825.425 kb on - strand, within Pf6N2E2_2387 at 2825.425 kb on - strand, within Pf6N2E2_2387 at 2825.574 kb on + strand, within Pf6N2E2_2387 at 2825.844 kb on + strand at 2825.845 kb on - strand at 2825.930 kb on - strand at 2825.930 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,823,355 - Pf6N2E2_2384 0.18 +0.1 2,823,370 - Pf6N2E2_2384 0.20 -0.2 2,823,370 - Pf6N2E2_2384 0.20 -0.8 2,823,370 - Pf6N2E2_2384 0.20 +2.3 2,823,518 - Pf6N2E2_2384 0.38 +1.8 2,823,518 - Pf6N2E2_2384 0.38 +1.1 2,823,518 - Pf6N2E2_2384 0.38 +1.3 2,823,689 - Pf6N2E2_2384 0.59 -1.8 2,823,756 - Pf6N2E2_2384 0.67 -2.5 2,823,756 - Pf6N2E2_2384 0.67 +1.0 2,823,756 - Pf6N2E2_2384 0.67 -0.1 2,823,965 - -1.4 2,823,965 - +1.2 2,823,969 - +0.5 2,824,030 + -1.8 2,824,031 - -0.2 2,824,045 - -0.5 2,824,060 - Pf6N2E2_2385 0.11 -1.2 2,824,523 + Pf6N2E2_2386 0.18 -2.2 2,824,523 + Pf6N2E2_2386 0.18 -2.0 2,824,524 - Pf6N2E2_2386 0.18 +0.1 2,824,650 - Pf6N2E2_2386 0.31 -1.2 2,824,787 + Pf6N2E2_2386 0.45 -1.7 2,824,787 + Pf6N2E2_2386 0.45 -1.2 2,824,788 - Pf6N2E2_2386 0.45 +0.0 2,824,788 - Pf6N2E2_2386 0.45 +0.4 2,824,788 - Pf6N2E2_2386 0.45 -0.7 2,824,886 + Pf6N2E2_2386 0.55 -0.0 2,824,887 - Pf6N2E2_2386 0.55 -0.9 2,825,356 + -1.6 2,825,356 + -1.5 2,825,357 - +0.4 2,825,419 - Pf6N2E2_2387 0.11 -0.3 2,825,419 - Pf6N2E2_2387 0.11 +3.1 2,825,419 - Pf6N2E2_2387 0.11 -0.9 2,825,419 - Pf6N2E2_2387 0.11 -0.3 2,825,424 + Pf6N2E2_2387 0.12 -3.0 2,825,424 + Pf6N2E2_2387 0.12 -2.3 2,825,424 + Pf6N2E2_2387 0.12 -1.3 2,825,425 - Pf6N2E2_2387 0.13 -0.2 2,825,425 - Pf6N2E2_2387 0.13 -0.4 2,825,574 + Pf6N2E2_2387 0.43 -1.2 2,825,844 + -1.1 2,825,845 - +1.3 2,825,930 - -1.0 2,825,930 - -0.3
Or see this region's nucleotide sequence