Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2272 and Pf6N2E2_2273 overlap by 4 nucleotides Pf6N2E2_2273 and Pf6N2E2_2274 are separated by 42 nucleotides Pf6N2E2_2274 and Pf6N2E2_2275 are separated by 346 nucleotides
Pf6N2E2_2272 - FIG00953243: hypothetical protein, at 2,686,479 to 2,687,075
_2272
Pf6N2E2_2273 - Glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46), at 2,687,072 to 2,687,794
_2273
Pf6N2E2_2274 - Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1), at 2,687,837 to 2,689,231
_2274
Pf6N2E2_2275 - Thiamin biosynthesis lipoprotein ApbE, at 2,689,578 to 2,690,567
_2275
Position (kb)
2687
2688
2689
2690 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2687.055 kb on - strand at 2687.060 kb on + strand at 2687.061 kb on - strand at 2687.061 kb on - strand at 2687.084 kb on + strand at 2687.084 kb on + strand at 2687.084 kb on + strand at 2687.084 kb on + strand at 2687.084 kb on + strand at 2687.085 kb on - strand at 2687.160 kb on + strand, within Pf6N2E2_2273 at 2687.161 kb on - strand, within Pf6N2E2_2273 at 2687.291 kb on + strand, within Pf6N2E2_2273 at 2687.291 kb on + strand, within Pf6N2E2_2273 at 2687.291 kb on + strand, within Pf6N2E2_2273 at 2687.291 kb on + strand, within Pf6N2E2_2273 at 2687.292 kb on - strand, within Pf6N2E2_2273 at 2687.292 kb on - strand, within Pf6N2E2_2273 at 2687.292 kb on - strand, within Pf6N2E2_2273 at 2687.292 kb on - strand, within Pf6N2E2_2273 at 2687.597 kb on + strand, within Pf6N2E2_2273 at 2687.598 kb on - strand, within Pf6N2E2_2273 at 2687.749 kb on + strand at 2687.749 kb on + strand at 2687.862 kb on + strand at 2687.863 kb on - strand at 2688.018 kb on - strand, within Pf6N2E2_2274 at 2688.186 kb on + strand, within Pf6N2E2_2274 at 2688.187 kb on - strand, within Pf6N2E2_2274 at 2688.187 kb on - strand, within Pf6N2E2_2274 at 2688.238 kb on + strand, within Pf6N2E2_2274 at 2688.239 kb on - strand, within Pf6N2E2_2274 at 2688.315 kb on + strand, within Pf6N2E2_2274 at 2688.316 kb on - strand, within Pf6N2E2_2274 at 2688.316 kb on - strand, within Pf6N2E2_2274 at 2688.316 kb on - strand, within Pf6N2E2_2274 at 2688.327 kb on - strand, within Pf6N2E2_2274 at 2688.335 kb on - strand, within Pf6N2E2_2274 at 2688.335 kb on - strand, within Pf6N2E2_2274 at 2689.216 kb on - strand at 2689.221 kb on + strand at 2689.235 kb on + strand at 2689.235 kb on + strand at 2689.236 kb on - strand at 2689.344 kb on + strand at 2689.344 kb on + strand at 2689.344 kb on + strand at 2689.345 kb on - strand at 2689.345 kb on - strand at 2689.345 kb on - strand at 2689.345 kb on - strand at 2689.345 kb on - strand at 2689.345 kb on - strand at 2689.347 kb on - strand at 2689.347 kb on - strand at 2689.347 kb on - strand at 2689.347 kb on - strand at 2689.347 kb on - strand at 2689.347 kb on - strand at 2689.389 kb on - strand at 2689.408 kb on + strand at 2689.422 kb on + strand at 2689.423 kb on - strand at 2689.560 kb on - strand at 2689.560 kb on - strand at 2689.560 kb on - strand at 2689.687 kb on + strand, within Pf6N2E2_2275 at 2689.688 kb on - strand, within Pf6N2E2_2275 at 2689.788 kb on + strand, within Pf6N2E2_2275 at 2689.788 kb on + strand, within Pf6N2E2_2275 at 2689.788 kb on + strand, within Pf6N2E2_2275 at 2689.789 kb on - strand, within Pf6N2E2_2275 at 2689.789 kb on - strand, within Pf6N2E2_2275 at 2689.852 kb on + strand, within Pf6N2E2_2275 at 2689.853 kb on - strand, within Pf6N2E2_2275 at 2689.856 kb on + strand, within Pf6N2E2_2275 at 2689.856 kb on + strand, within Pf6N2E2_2275 at 2689.856 kb on + strand, within Pf6N2E2_2275 at 2689.857 kb on - strand, within Pf6N2E2_2275 at 2690.014 kb on + strand, within Pf6N2E2_2275 at 2690.014 kb on + strand, within Pf6N2E2_2275 at 2690.015 kb on - strand, within Pf6N2E2_2275 at 2690.015 kb on - strand, within Pf6N2E2_2275 at 2690.191 kb on - strand, within Pf6N2E2_2275
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,687,055 - +0.5 2,687,060 + -1.0 2,687,061 - -0.6 2,687,061 - -1.0 2,687,084 + +0.5 2,687,084 + +0.2 2,687,084 + -0.0 2,687,084 + -0.8 2,687,084 + +0.7 2,687,085 - +1.2 2,687,160 + Pf6N2E2_2273 0.12 -0.8 2,687,161 - Pf6N2E2_2273 0.12 -0.2 2,687,291 + Pf6N2E2_2273 0.30 -0.3 2,687,291 + Pf6N2E2_2273 0.30 +0.4 2,687,291 + Pf6N2E2_2273 0.30 -0.3 2,687,291 + Pf6N2E2_2273 0.30 +0.2 2,687,292 - Pf6N2E2_2273 0.30 -0.0 2,687,292 - Pf6N2E2_2273 0.30 +0.8 2,687,292 - Pf6N2E2_2273 0.30 +0.3 2,687,292 - Pf6N2E2_2273 0.30 -0.1 2,687,597 + Pf6N2E2_2273 0.73 +0.5 2,687,598 - Pf6N2E2_2273 0.73 +1.1 2,687,749 + +0.2 2,687,749 + +0.9 2,687,862 + -3.2 2,687,863 - -0.2 2,688,018 - Pf6N2E2_2274 0.13 -0.8 2,688,186 + Pf6N2E2_2274 0.25 -1.6 2,688,187 - Pf6N2E2_2274 0.25 -1.6 2,688,187 - Pf6N2E2_2274 0.25 -1.0 2,688,238 + Pf6N2E2_2274 0.29 -2.3 2,688,239 - Pf6N2E2_2274 0.29 -2.1 2,688,315 + Pf6N2E2_2274 0.34 +0.8 2,688,316 - Pf6N2E2_2274 0.34 -0.7 2,688,316 - Pf6N2E2_2274 0.34 -1.2 2,688,316 - Pf6N2E2_2274 0.34 -2.5 2,688,327 - Pf6N2E2_2274 0.35 -2.8 2,688,335 - Pf6N2E2_2274 0.36 -3.5 2,688,335 - Pf6N2E2_2274 0.36 -3.0 2,689,216 - +2.3 2,689,221 + +0.2 2,689,235 + -1.5 2,689,235 + +0.2 2,689,236 - -1.5 2,689,344 + -2.1 2,689,344 + -2.5 2,689,344 + -1.2 2,689,345 - +0.8 2,689,345 - +0.1 2,689,345 - +0.5 2,689,345 - +0.3 2,689,345 - -0.0 2,689,345 - +0.2 2,689,347 - +1.1 2,689,347 - -0.4 2,689,347 - +0.7 2,689,347 - +1.1 2,689,347 - +0.9 2,689,347 - +1.5 2,689,389 - -0.8 2,689,408 + -1.0 2,689,422 + +0.3 2,689,423 - +0.2 2,689,560 - -1.9 2,689,560 - -0.2 2,689,560 - -0.0 2,689,687 + Pf6N2E2_2275 0.11 -0.2 2,689,688 - Pf6N2E2_2275 0.11 +1.5 2,689,788 + Pf6N2E2_2275 0.21 +0.9 2,689,788 + Pf6N2E2_2275 0.21 +2.0 2,689,788 + Pf6N2E2_2275 0.21 -0.1 2,689,789 - Pf6N2E2_2275 0.21 +0.3 2,689,789 - Pf6N2E2_2275 0.21 +0.4 2,689,852 + Pf6N2E2_2275 0.28 -1.0 2,689,853 - Pf6N2E2_2275 0.28 +1.5 2,689,856 + Pf6N2E2_2275 0.28 -0.2 2,689,856 + Pf6N2E2_2275 0.28 +0.4 2,689,856 + Pf6N2E2_2275 0.28 -0.3 2,689,857 - Pf6N2E2_2275 0.28 +1.1 2,690,014 + Pf6N2E2_2275 0.44 +0.7 2,690,014 + Pf6N2E2_2275 0.44 +0.6 2,690,015 - Pf6N2E2_2275 0.44 +0.7 2,690,015 - Pf6N2E2_2275 0.44 +1.5 2,690,191 - Pf6N2E2_2275 0.62 +1.0
Or see this region's nucleotide sequence