Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2212 and Pf6N2E2_2213 are separated by 303 nucleotides Pf6N2E2_2213 and Pf6N2E2_2214 are separated by 369 nucleotides
Pf6N2E2_2212 - Transcriptional regulator, AraC family, at 2,620,644 to 2,621,666
_2212
Pf6N2E2_2213 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 2,621,970 to 2,622,851
_2213
Pf6N2E2_2214 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 2,623,221 to 2,625,092
_2214
Position (kb)
2621
2622
2623 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2620.974 kb on + strand, within Pf6N2E2_2212 at 2620.975 kb on - strand, within Pf6N2E2_2212 at 2621.028 kb on + strand, within Pf6N2E2_2212 at 2621.029 kb on - strand, within Pf6N2E2_2212 at 2621.029 kb on - strand, within Pf6N2E2_2212 at 2621.043 kb on + strand, within Pf6N2E2_2212 at 2621.044 kb on - strand, within Pf6N2E2_2212 at 2621.052 kb on + strand, within Pf6N2E2_2212 at 2621.052 kb on + strand, within Pf6N2E2_2212 at 2621.166 kb on + strand, within Pf6N2E2_2212 at 2621.166 kb on + strand, within Pf6N2E2_2212 at 2621.167 kb on - strand, within Pf6N2E2_2212 at 2621.167 kb on - strand, within Pf6N2E2_2212 at 2621.167 kb on - strand, within Pf6N2E2_2212 at 2621.167 kb on - strand, within Pf6N2E2_2212 at 2621.167 kb on - strand, within Pf6N2E2_2212 at 2621.526 kb on + strand, within Pf6N2E2_2212 at 2621.526 kb on + strand, within Pf6N2E2_2212 at 2621.526 kb on + strand, within Pf6N2E2_2212 at 2621.527 kb on - strand, within Pf6N2E2_2212 at 2621.527 kb on - strand, within Pf6N2E2_2212 at 2621.527 kb on - strand, within Pf6N2E2_2212 at 2621.527 kb on - strand, within Pf6N2E2_2212 at 2621.527 kb on - strand, within Pf6N2E2_2212 at 2621.527 kb on - strand, within Pf6N2E2_2212 at 2621.527 kb on - strand, within Pf6N2E2_2212 at 2621.527 kb on - strand, within Pf6N2E2_2212 at 2621.527 kb on - strand, within Pf6N2E2_2212 at 2621.530 kb on + strand, within Pf6N2E2_2212 at 2621.531 kb on - strand, within Pf6N2E2_2212 at 2621.531 kb on - strand, within Pf6N2E2_2212 at 2621.565 kb on + strand at 2621.565 kb on + strand at 2621.565 kb on + strand at 2621.566 kb on - strand at 2621.569 kb on + strand at 2621.569 kb on + strand at 2621.649 kb on - strand at 2621.683 kb on + strand at 2621.684 kb on - strand at 2621.692 kb on - strand at 2621.692 kb on - strand at 2621.692 kb on - strand at 2621.700 kb on + strand at 2621.700 kb on + strand at 2621.700 kb on + strand at 2621.701 kb on - strand at 2621.701 kb on - strand at 2621.717 kb on + strand at 2621.718 kb on - strand at 2621.718 kb on - strand at 2621.718 kb on - strand at 2621.718 kb on - strand at 2621.718 kb on - strand at 2621.718 kb on - strand at 2621.762 kb on + strand at 2621.763 kb on - strand at 2621.772 kb on - strand at 2621.792 kb on + strand at 2621.803 kb on + strand at 2621.803 kb on + strand at 2621.803 kb on + strand at 2621.803 kb on + strand at 2621.804 kb on - strand at 2621.804 kb on - strand at 2621.804 kb on - strand at 2621.857 kb on + strand at 2621.857 kb on + strand at 2621.868 kb on + strand at 2621.868 kb on + strand at 2621.868 kb on + strand at 2621.869 kb on - strand at 2621.875 kb on - strand at 2621.902 kb on + strand at 2621.903 kb on - strand at 2621.927 kb on + strand at 2621.928 kb on - strand at 2621.928 kb on - strand at 2621.928 kb on - strand at 2621.934 kb on + strand at 2622.367 kb on + strand, within Pf6N2E2_2213 at 2622.367 kb on + strand, within Pf6N2E2_2213 at 2622.367 kb on + strand, within Pf6N2E2_2213 at 2622.367 kb on + strand, within Pf6N2E2_2213 at 2622.368 kb on - strand, within Pf6N2E2_2213 at 2622.368 kb on - strand, within Pf6N2E2_2213 at 2622.368 kb on - strand, within Pf6N2E2_2213 at 2622.658 kb on + strand, within Pf6N2E2_2213 at 2622.659 kb on - strand, within Pf6N2E2_2213 at 2622.659 kb on - strand, within Pf6N2E2_2213 at 2622.659 kb on - strand, within Pf6N2E2_2213 at 2622.718 kb on + strand, within Pf6N2E2_2213 at 2622.718 kb on + strand, within Pf6N2E2_2213 at 2622.763 kb on + strand at 2622.764 kb on - strand at 2622.882 kb on + strand at 2622.883 kb on - strand at 2622.883 kb on - strand at 2622.982 kb on + strand at 2622.982 kb on + strand at 2623.103 kb on + strand at 2623.113 kb on + strand at 2623.113 kb on + strand at 2623.113 kb on + strand at 2623.113 kb on + strand at 2623.113 kb on + strand at 2623.113 kb on + strand at 2623.113 kb on + strand at 2623.113 kb on + strand at 2623.114 kb on - strand at 2623.114 kb on - strand at 2623.192 kb on - strand at 2623.192 kb on - strand at 2623.192 kb on - strand at 2623.192 kb on - strand at 2623.192 kb on - strand at 2623.192 kb on - strand at 2623.222 kb on + strand at 2623.223 kb on - strand at 2623.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,620,974 + Pf6N2E2_2212 0.32 +0.9 2,620,975 - Pf6N2E2_2212 0.32 -1.2 2,621,028 + Pf6N2E2_2212 0.38 -0.4 2,621,029 - Pf6N2E2_2212 0.38 +0.8 2,621,029 - Pf6N2E2_2212 0.38 -1.0 2,621,043 + Pf6N2E2_2212 0.39 +0.4 2,621,044 - Pf6N2E2_2212 0.39 +0.5 2,621,052 + Pf6N2E2_2212 0.40 +0.4 2,621,052 + Pf6N2E2_2212 0.40 -0.3 2,621,166 + Pf6N2E2_2212 0.51 -0.1 2,621,166 + Pf6N2E2_2212 0.51 +0.6 2,621,167 - Pf6N2E2_2212 0.51 -0.1 2,621,167 - Pf6N2E2_2212 0.51 -0.5 2,621,167 - Pf6N2E2_2212 0.51 +0.6 2,621,167 - Pf6N2E2_2212 0.51 -0.6 2,621,167 - Pf6N2E2_2212 0.51 -0.2 2,621,526 + Pf6N2E2_2212 0.86 -0.6 2,621,526 + Pf6N2E2_2212 0.86 -1.8 2,621,526 + Pf6N2E2_2212 0.86 -0.2 2,621,527 - Pf6N2E2_2212 0.86 -0.2 2,621,527 - Pf6N2E2_2212 0.86 -0.5 2,621,527 - Pf6N2E2_2212 0.86 -0.7 2,621,527 - Pf6N2E2_2212 0.86 -0.1 2,621,527 - Pf6N2E2_2212 0.86 -0.9 2,621,527 - Pf6N2E2_2212 0.86 +1.3 2,621,527 - Pf6N2E2_2212 0.86 -0.2 2,621,527 - Pf6N2E2_2212 0.86 +0.6 2,621,527 - Pf6N2E2_2212 0.86 -0.4 2,621,530 + Pf6N2E2_2212 0.87 +0.8 2,621,531 - Pf6N2E2_2212 0.87 -2.8 2,621,531 - Pf6N2E2_2212 0.87 -0.9 2,621,565 + -3.2 2,621,565 + +0.1 2,621,565 + +0.3 2,621,566 - -0.9 2,621,569 + -1.1 2,621,569 + -0.2 2,621,649 - -1.3 2,621,683 + -1.9 2,621,684 - -0.4 2,621,692 - -1.1 2,621,692 - -0.4 2,621,692 - -0.2 2,621,700 + +1.6 2,621,700 + +1.2 2,621,700 + -1.1 2,621,701 - -2.2 2,621,701 - -0.3 2,621,717 + -0.2 2,621,718 - -3.2 2,621,718 - +0.6 2,621,718 - -0.2 2,621,718 - -0.8 2,621,718 - -2.0 2,621,718 - -0.9 2,621,762 + -0.7 2,621,763 - -1.5 2,621,772 - +1.4 2,621,792 + -0.5 2,621,803 + -3.9 2,621,803 + -0.2 2,621,803 + -3.0 2,621,803 + +0.1 2,621,804 - -2.5 2,621,804 - -0.5 2,621,804 - -0.6 2,621,857 + -0.7 2,621,857 + -1.6 2,621,868 + +0.4 2,621,868 + -1.5 2,621,868 + -0.4 2,621,869 - -1.8 2,621,875 - +0.1 2,621,902 + -1.0 2,621,903 - -0.0 2,621,927 + +0.1 2,621,928 - -1.1 2,621,928 - -1.2 2,621,928 - +0.5 2,621,934 + -0.8 2,622,367 + Pf6N2E2_2213 0.45 -2.8 2,622,367 + Pf6N2E2_2213 0.45 -2.9 2,622,367 + Pf6N2E2_2213 0.45 -0.8 2,622,367 + Pf6N2E2_2213 0.45 -0.2 2,622,368 - Pf6N2E2_2213 0.45 -0.3 2,622,368 - Pf6N2E2_2213 0.45 +0.1 2,622,368 - Pf6N2E2_2213 0.45 -0.0 2,622,658 + Pf6N2E2_2213 0.78 -1.2 2,622,659 - Pf6N2E2_2213 0.78 +0.8 2,622,659 - Pf6N2E2_2213 0.78 -1.2 2,622,659 - Pf6N2E2_2213 0.78 -0.9 2,622,718 + Pf6N2E2_2213 0.85 -0.4 2,622,718 + Pf6N2E2_2213 0.85 -1.5 2,622,763 + -1.3 2,622,764 - -1.5 2,622,882 + +0.2 2,622,883 - -3.5 2,622,883 - -2.3 2,622,982 + +0.6 2,622,982 + -3.1 2,623,103 + -0.8 2,623,113 + -1.1 2,623,113 + -0.5 2,623,113 + -0.1 2,623,113 + -2.0 2,623,113 + +0.2 2,623,113 + -0.5 2,623,113 + -0.7 2,623,113 + -1.2 2,623,114 - -0.1 2,623,114 - -0.2 2,623,192 - -0.2 2,623,192 - -0.2 2,623,192 - -1.2 2,623,192 - -0.2 2,623,192 - +0.1 2,623,192 - -0.9 2,623,222 + -1.5 2,623,223 - +0.3 2,623,313 - +0.8
Or see this region's nucleotide sequence