Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2181 and Pf6N2E2_2182 are separated by 133 nucleotides Pf6N2E2_2182 and Pf6N2E2_2183 overlap by 4 nucleotides Pf6N2E2_2183 and Pf6N2E2_2184 are separated by 26 nucleotides
Pf6N2E2_2181 - Arginine N-succinyltransferase (EC 2.3.1.109), at 2,586,219 to 2,587,253
_2181
Pf6N2E2_2182 - hypothetical protein, at 2,587,387 to 2,588,166
_2182
Pf6N2E2_2183 - hypothetical protein, at 2,588,163 to 2,588,825
_2183
Pf6N2E2_2184 - hypothetical protein, at 2,588,852 to 2,589,031
_2184
Position (kb)
2587
2588
2589 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2586.472 kb on - strand at 2586.472 kb on - strand, within Pf6N2E2_2181 at 2586.477 kb on + strand, within Pf6N2E2_2181 at 2586.850 kb on - strand, within Pf6N2E2_2181 at 2587.285 kb on - strand at 2587.285 kb on - strand at 2587.289 kb on - strand at 2587.289 kb on - strand at 2587.289 kb on - strand at 2587.329 kb on - strand at 2587.329 kb on - strand at 2587.357 kb on - strand at 2587.434 kb on + strand at 2587.457 kb on + strand at 2587.652 kb on - strand, within Pf6N2E2_2182 at 2587.671 kb on + strand, within Pf6N2E2_2182 at 2587.712 kb on - strand, within Pf6N2E2_2182 at 2587.884 kb on - strand, within Pf6N2E2_2182 at 2587.884 kb on - strand, within Pf6N2E2_2182 at 2587.884 kb on - strand, within Pf6N2E2_2182 at 2588.017 kb on + strand, within Pf6N2E2_2182 at 2588.017 kb on + strand, within Pf6N2E2_2182 at 2588.018 kb on - strand, within Pf6N2E2_2182 at 2588.024 kb on - strand, within Pf6N2E2_2182 at 2588.092 kb on + strand at 2588.093 kb on - strand at 2588.093 kb on - strand at 2588.199 kb on + strand at 2588.199 kb on + strand at 2588.200 kb on - strand at 2588.200 kb on - strand at 2588.283 kb on + strand, within Pf6N2E2_2183 at 2588.284 kb on - strand, within Pf6N2E2_2183 at 2588.286 kb on - strand, within Pf6N2E2_2183 at 2588.328 kb on + strand, within Pf6N2E2_2183 at 2588.329 kb on - strand, within Pf6N2E2_2183 at 2588.338 kb on - strand, within Pf6N2E2_2183 at 2588.490 kb on + strand, within Pf6N2E2_2183 at 2588.503 kb on + strand, within Pf6N2E2_2183 at 2588.525 kb on - strand, within Pf6N2E2_2183 at 2588.574 kb on - strand, within Pf6N2E2_2183 at 2588.597 kb on - strand, within Pf6N2E2_2183 at 2588.597 kb on - strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.598 kb on + strand, within Pf6N2E2_2183 at 2588.599 kb on - strand, within Pf6N2E2_2183 at 2588.599 kb on - strand, within Pf6N2E2_2183 at 2588.599 kb on - strand, within Pf6N2E2_2183 at 2588.599 kb on - strand, within Pf6N2E2_2183 at 2588.599 kb on - strand, within Pf6N2E2_2183 at 2588.724 kb on + strand, within Pf6N2E2_2183 at 2588.724 kb on + strand, within Pf6N2E2_2183 at 2588.724 kb on + strand, within Pf6N2E2_2183 at 2588.724 kb on + strand, within Pf6N2E2_2183 at 2588.725 kb on - strand, within Pf6N2E2_2183 at 2588.725 kb on - strand, within Pf6N2E2_2183 at 2588.725 kb on - strand, within Pf6N2E2_2183 at 2588.725 kb on - strand, within Pf6N2E2_2183 at 2588.728 kb on + strand, within Pf6N2E2_2183 at 2588.729 kb on - strand, within Pf6N2E2_2183 at 2588.729 kb on - strand, within Pf6N2E2_2183 at 2588.746 kb on + strand, within Pf6N2E2_2183 at 2588.782 kb on + strand at 2588.782 kb on + strand at 2588.782 kb on + strand at 2588.782 kb on + strand at 2588.782 kb on + strand at 2588.783 kb on - strand at 2588.783 kb on - strand at 2588.850 kb on + strand at 2588.850 kb on + strand at 2589.021 kb on + strand at 2589.022 kb on - strand at 2589.022 kb on - strand at 2589.022 kb on - strand at 2589.024 kb on + strand at 2589.024 kb on + strand at 2589.025 kb on - strand at 2589.025 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,586,472 - -0.2 2,586,472 - Pf6N2E2_2181 0.24 -0.6 2,586,477 + Pf6N2E2_2181 0.25 -0.8 2,586,850 - Pf6N2E2_2181 0.61 -0.2 2,587,285 - -0.4 2,587,285 - -0.2 2,587,289 - +0.3 2,587,289 - +0.1 2,587,289 - +0.8 2,587,329 - +0.2 2,587,329 - -4.0 2,587,357 - -0.0 2,587,434 + +1.5 2,587,457 + +0.1 2,587,652 - Pf6N2E2_2182 0.34 +2.1 2,587,671 + Pf6N2E2_2182 0.36 -0.7 2,587,712 - Pf6N2E2_2182 0.42 -2.5 2,587,884 - Pf6N2E2_2182 0.64 -1.2 2,587,884 - Pf6N2E2_2182 0.64 -1.4 2,587,884 - Pf6N2E2_2182 0.64 -0.0 2,588,017 + Pf6N2E2_2182 0.81 -1.5 2,588,017 + Pf6N2E2_2182 0.81 -1.2 2,588,018 - Pf6N2E2_2182 0.81 -2.1 2,588,024 - Pf6N2E2_2182 0.82 -0.5 2,588,092 + +2.3 2,588,093 - -0.2 2,588,093 - -0.0 2,588,199 + -1.3 2,588,199 + -0.5 2,588,200 - -2.0 2,588,200 - +0.5 2,588,283 + Pf6N2E2_2183 0.18 +0.6 2,588,284 - Pf6N2E2_2183 0.18 -0.5 2,588,286 - Pf6N2E2_2183 0.19 +1.5 2,588,328 + Pf6N2E2_2183 0.25 -0.2 2,588,329 - Pf6N2E2_2183 0.25 +1.0 2,588,338 - Pf6N2E2_2183 0.26 +1.3 2,588,490 + Pf6N2E2_2183 0.49 +0.4 2,588,503 + Pf6N2E2_2183 0.51 +1.4 2,588,525 - Pf6N2E2_2183 0.55 +0.9 2,588,574 - Pf6N2E2_2183 0.62 +0.8 2,588,597 - Pf6N2E2_2183 0.65 +0.4 2,588,597 - Pf6N2E2_2183 0.65 +0.5 2,588,598 + Pf6N2E2_2183 0.66 -2.4 2,588,598 + Pf6N2E2_2183 0.66 -0.1 2,588,598 + Pf6N2E2_2183 0.66 +0.7 2,588,598 + Pf6N2E2_2183 0.66 -0.8 2,588,598 + Pf6N2E2_2183 0.66 +0.3 2,588,598 + Pf6N2E2_2183 0.66 +1.0 2,588,598 + Pf6N2E2_2183 0.66 +1.4 2,588,599 - Pf6N2E2_2183 0.66 -1.1 2,588,599 - Pf6N2E2_2183 0.66 +0.5 2,588,599 - Pf6N2E2_2183 0.66 +0.9 2,588,599 - Pf6N2E2_2183 0.66 -0.3 2,588,599 - Pf6N2E2_2183 0.66 +1.6 2,588,724 + Pf6N2E2_2183 0.85 +0.4 2,588,724 + Pf6N2E2_2183 0.85 +0.1 2,588,724 + Pf6N2E2_2183 0.85 -0.3 2,588,724 + Pf6N2E2_2183 0.85 -0.4 2,588,725 - Pf6N2E2_2183 0.85 -0.5 2,588,725 - Pf6N2E2_2183 0.85 -0.6 2,588,725 - Pf6N2E2_2183 0.85 +3.7 2,588,725 - Pf6N2E2_2183 0.85 +0.5 2,588,728 + Pf6N2E2_2183 0.85 +0.3 2,588,729 - Pf6N2E2_2183 0.85 +0.4 2,588,729 - Pf6N2E2_2183 0.85 -1.5 2,588,746 + Pf6N2E2_2183 0.88 +0.7 2,588,782 + -0.5 2,588,782 + +0.0 2,588,782 + -1.2 2,588,782 + -0.0 2,588,782 + +0.5 2,588,783 - -2.8 2,588,783 - -0.6 2,588,850 + +0.3 2,588,850 + -0.6 2,589,021 + +0.7 2,589,022 - +2.8 2,589,022 - -0.8 2,589,022 - -0.2 2,589,024 + +0.4 2,589,024 + +0.5 2,589,025 - +0.8 2,589,025 - -0.2
Or see this region's nucleotide sequence