Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2024 and Pf6N2E2_2025 are separated by 613 nucleotides Pf6N2E2_2025 and Pf6N2E2_2026 are separated by 65 nucleotides Pf6N2E2_2026 and Pf6N2E2_2027 are separated by 45 nucleotides
Pf6N2E2_2024 - Hydrolase, carbon-nitrogen family, at 2,412,607 to 2,413,368
_2024
Pf6N2E2_2025 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 2,413,982 to 2,415,052
_2025
Pf6N2E2_2026 - hypothetical protein, at 2,415,118 to 2,415,924
_2026
Pf6N2E2_2027 - hypothetical protein, at 2,415,970 to 2,416,140
_2027
Position (kb)
2413
2414
2415
2416 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2413.038 kb on - strand, within Pf6N2E2_2024 at 2413.038 kb on - strand, within Pf6N2E2_2024 at 2413.246 kb on + strand, within Pf6N2E2_2024 at 2413.409 kb on + strand at 2413.410 kb on - strand at 2413.490 kb on + strand at 2413.490 kb on + strand at 2413.493 kb on + strand at 2413.493 kb on + strand at 2413.493 kb on + strand at 2413.494 kb on - strand at 2413.494 kb on - strand at 2413.494 kb on - strand at 2413.494 kb on - strand at 2413.494 kb on - strand at 2413.617 kb on + strand at 2413.790 kb on + strand at 2413.790 kb on + strand at 2413.791 kb on - strand at 2413.791 kb on - strand at 2413.799 kb on + strand at 2413.799 kb on + strand at 2413.799 kb on + strand at 2413.799 kb on + strand at 2413.799 kb on + strand at 2413.800 kb on - strand at 2413.800 kb on - strand at 2413.800 kb on - strand at 2413.800 kb on - strand at 2413.800 kb on - strand at 2413.800 kb on - strand at 2413.800 kb on - strand at 2413.835 kb on - strand at 2413.879 kb on + strand at 2413.934 kb on + strand at 2413.934 kb on + strand at 2413.934 kb on + strand at 2413.935 kb on - strand at 2414.219 kb on + strand, within Pf6N2E2_2025 at 2414.286 kb on - strand, within Pf6N2E2_2025 at 2414.286 kb on - strand, within Pf6N2E2_2025 at 2414.330 kb on + strand, within Pf6N2E2_2025 at 2414.459 kb on + strand, within Pf6N2E2_2025 at 2414.460 kb on - strand, within Pf6N2E2_2025 at 2414.729 kb on + strand, within Pf6N2E2_2025 at 2414.730 kb on - strand, within Pf6N2E2_2025 at 2414.730 kb on - strand, within Pf6N2E2_2025 at 2414.730 kb on - strand, within Pf6N2E2_2025 at 2414.754 kb on + strand, within Pf6N2E2_2025 at 2414.754 kb on + strand, within Pf6N2E2_2025 at 2414.754 kb on + strand, within Pf6N2E2_2025 at 2414.754 kb on + strand, within Pf6N2E2_2025 at 2414.754 kb on + strand, within Pf6N2E2_2025 at 2414.755 kb on - strand, within Pf6N2E2_2025 at 2414.755 kb on - strand, within Pf6N2E2_2025 at 2414.755 kb on - strand, within Pf6N2E2_2025 at 2415.198 kb on - strand at 2415.198 kb on - strand at 2415.198 kb on - strand at 2415.236 kb on + strand, within Pf6N2E2_2026 at 2415.236 kb on + strand, within Pf6N2E2_2026 at 2415.236 kb on + strand, within Pf6N2E2_2026 at 2415.237 kb on - strand, within Pf6N2E2_2026 at 2415.237 kb on - strand, within Pf6N2E2_2026 at 2415.305 kb on + strand, within Pf6N2E2_2026 at 2415.305 kb on + strand, within Pf6N2E2_2026 at 2415.305 kb on + strand, within Pf6N2E2_2026 at 2415.306 kb on - strand, within Pf6N2E2_2026 at 2415.306 kb on - strand, within Pf6N2E2_2026 at 2415.435 kb on + strand, within Pf6N2E2_2026 at 2415.524 kb on + strand, within Pf6N2E2_2026 at 2415.524 kb on + strand, within Pf6N2E2_2026 at 2415.524 kb on + strand, within Pf6N2E2_2026 at 2415.524 kb on + strand, within Pf6N2E2_2026 at 2415.525 kb on - strand, within Pf6N2E2_2026 at 2415.525 kb on - strand, within Pf6N2E2_2026 at 2415.525 kb on - strand, within Pf6N2E2_2026 at 2415.525 kb on - strand, within Pf6N2E2_2026 at 2415.547 kb on + strand, within Pf6N2E2_2026 at 2415.547 kb on + strand, within Pf6N2E2_2026 at 2415.547 kb on + strand, within Pf6N2E2_2026 at 2415.547 kb on + strand, within Pf6N2E2_2026 at 2415.547 kb on + strand, within Pf6N2E2_2026 at 2415.547 kb on + strand, within Pf6N2E2_2026 at 2415.548 kb on - strand, within Pf6N2E2_2026 at 2415.548 kb on - strand, within Pf6N2E2_2026 at 2415.548 kb on - strand, within Pf6N2E2_2026 at 2415.613 kb on + strand, within Pf6N2E2_2026 at 2415.613 kb on + strand, within Pf6N2E2_2026 at 2415.613 kb on + strand, within Pf6N2E2_2026 at 2415.614 kb on - strand, within Pf6N2E2_2026 at 2415.793 kb on + strand, within Pf6N2E2_2026 at 2415.794 kb on - strand, within Pf6N2E2_2026 at 2415.850 kb on - strand at 2415.938 kb on - strand at 2415.977 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,413,038 - Pf6N2E2_2024 0.57 +0.6 2,413,038 - Pf6N2E2_2024 0.57 +0.7 2,413,246 + Pf6N2E2_2024 0.84 +0.1 2,413,409 + +0.7 2,413,410 - +1.8 2,413,490 + -0.9 2,413,490 + -1.0 2,413,493 + +0.8 2,413,493 + +0.8 2,413,493 + -2.3 2,413,494 - -0.5 2,413,494 - -1.2 2,413,494 - -0.4 2,413,494 - -0.9 2,413,494 - -0.2 2,413,617 + +2.2 2,413,790 + +2.8 2,413,790 + +1.7 2,413,791 - -0.4 2,413,791 - -1.5 2,413,799 + +1.8 2,413,799 + +2.9 2,413,799 + +2.2 2,413,799 + +3.1 2,413,799 + +1.6 2,413,800 - +0.8 2,413,800 - -0.7 2,413,800 - +0.8 2,413,800 - -1.5 2,413,800 - -0.7 2,413,800 - +2.3 2,413,800 - -0.2 2,413,835 - +0.6 2,413,879 + +1.7 2,413,934 + +1.7 2,413,934 + -0.9 2,413,934 + -0.2 2,413,935 - -1.3 2,414,219 + Pf6N2E2_2025 0.22 -0.6 2,414,286 - Pf6N2E2_2025 0.28 -1.6 2,414,286 - Pf6N2E2_2025 0.28 -0.4 2,414,330 + Pf6N2E2_2025 0.32 -0.9 2,414,459 + Pf6N2E2_2025 0.45 -0.2 2,414,460 - Pf6N2E2_2025 0.45 -1.6 2,414,729 + Pf6N2E2_2025 0.70 +0.1 2,414,730 - Pf6N2E2_2025 0.70 +0.6 2,414,730 - Pf6N2E2_2025 0.70 -1.2 2,414,730 - Pf6N2E2_2025 0.70 -0.7 2,414,754 + Pf6N2E2_2025 0.72 -0.4 2,414,754 + Pf6N2E2_2025 0.72 -0.3 2,414,754 + Pf6N2E2_2025 0.72 +0.3 2,414,754 + Pf6N2E2_2025 0.72 -1.6 2,414,754 + Pf6N2E2_2025 0.72 +1.4 2,414,755 - Pf6N2E2_2025 0.72 -0.2 2,414,755 - Pf6N2E2_2025 0.72 -2.5 2,414,755 - Pf6N2E2_2025 0.72 -0.4 2,415,198 - -0.3 2,415,198 - -1.2 2,415,198 - +0.1 2,415,236 + Pf6N2E2_2026 0.15 -1.7 2,415,236 + Pf6N2E2_2026 0.15 +0.6 2,415,236 + Pf6N2E2_2026 0.15 -0.5 2,415,237 - Pf6N2E2_2026 0.15 -0.1 2,415,237 - Pf6N2E2_2026 0.15 +0.9 2,415,305 + Pf6N2E2_2026 0.23 +1.2 2,415,305 + Pf6N2E2_2026 0.23 +1.3 2,415,305 + Pf6N2E2_2026 0.23 -2.0 2,415,306 - Pf6N2E2_2026 0.23 -0.7 2,415,306 - Pf6N2E2_2026 0.23 +0.8 2,415,435 + Pf6N2E2_2026 0.39 +2.3 2,415,524 + Pf6N2E2_2026 0.50 +0.3 2,415,524 + Pf6N2E2_2026 0.50 +1.6 2,415,524 + Pf6N2E2_2026 0.50 -0.6 2,415,524 + Pf6N2E2_2026 0.50 -1.2 2,415,525 - Pf6N2E2_2026 0.50 -1.2 2,415,525 - Pf6N2E2_2026 0.50 +0.3 2,415,525 - Pf6N2E2_2026 0.50 +0.0 2,415,525 - Pf6N2E2_2026 0.50 -0.2 2,415,547 + Pf6N2E2_2026 0.53 +0.8 2,415,547 + Pf6N2E2_2026 0.53 -0.7 2,415,547 + Pf6N2E2_2026 0.53 +0.7 2,415,547 + Pf6N2E2_2026 0.53 -0.2 2,415,547 + Pf6N2E2_2026 0.53 +0.4 2,415,547 + Pf6N2E2_2026 0.53 -0.5 2,415,548 - Pf6N2E2_2026 0.53 -0.2 2,415,548 - Pf6N2E2_2026 0.53 -0.8 2,415,548 - Pf6N2E2_2026 0.53 +2.0 2,415,613 + Pf6N2E2_2026 0.61 +0.6 2,415,613 + Pf6N2E2_2026 0.61 +0.7 2,415,613 + Pf6N2E2_2026 0.61 -1.0 2,415,614 - Pf6N2E2_2026 0.61 +2.5 2,415,793 + Pf6N2E2_2026 0.84 +0.2 2,415,794 - Pf6N2E2_2026 0.84 +0.3 2,415,850 - +0.2 2,415,938 - +0.3 2,415,977 + +0.3
Or see this region's nucleotide sequence