Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1983 and Pf6N2E2_1984 are separated by 8 nucleotides
Pf6N2E2_1983 - hypothetical protein, at 2,349,111 to 2,349,272
_1983
Pf6N2E2_1984 - probable exported protein STY4558, at 2,349,281 to 2,350,000
_1984
Position (kb)
2349
2350
2351 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2348.371 kb on + strand at 2348.371 kb on + strand at 2348.372 kb on - strand at 2348.372 kb on - strand at 2348.372 kb on - strand at 2348.590 kb on - strand at 2348.629 kb on + strand at 2348.629 kb on + strand at 2348.630 kb on - strand at 2348.630 kb on - strand at 2348.631 kb on + strand at 2348.631 kb on + strand at 2348.632 kb on - strand at 2348.632 kb on - strand at 2348.692 kb on + strand at 2348.693 kb on - strand at 2348.802 kb on - strand at 2348.857 kb on + strand at 2348.940 kb on - strand at 2348.940 kb on - strand at 2348.940 kb on - strand at 2349.136 kb on + strand, within Pf6N2E2_1983 at 2349.137 kb on - strand, within Pf6N2E2_1983 at 2349.187 kb on - strand, within Pf6N2E2_1983 at 2349.187 kb on - strand, within Pf6N2E2_1983 at 2349.187 kb on - strand, within Pf6N2E2_1983 at 2349.281 kb on - strand at 2349.281 kb on - strand at 2349.298 kb on - strand at 2349.494 kb on - strand, within Pf6N2E2_1984 at 2349.497 kb on + strand, within Pf6N2E2_1984 at 2349.497 kb on + strand at 2349.567 kb on - strand, within Pf6N2E2_1984 at 2349.598 kb on - strand, within Pf6N2E2_1984 at 2349.669 kb on + strand, within Pf6N2E2_1984 at 2349.859 kb on + strand, within Pf6N2E2_1984 at 2349.860 kb on - strand, within Pf6N2E2_1984 at 2349.904 kb on + strand, within Pf6N2E2_1984 at 2350.099 kb on + strand at 2350.787 kb on + strand at 2350.957 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,348,371 + -1.9 2,348,371 + -1.7 2,348,372 - +0.4 2,348,372 - -0.4 2,348,372 - -2.2 2,348,590 - -0.6 2,348,629 + -1.5 2,348,629 + -1.2 2,348,630 - +0.5 2,348,630 - +0.7 2,348,631 + -0.5 2,348,631 + -3.0 2,348,632 - +0.7 2,348,632 - -0.6 2,348,692 + +0.3 2,348,693 - -0.5 2,348,802 - -1.2 2,348,857 + -1.6 2,348,940 - +0.0 2,348,940 - -0.4 2,348,940 - -0.1 2,349,136 + Pf6N2E2_1983 0.15 -1.3 2,349,137 - Pf6N2E2_1983 0.16 -0.7 2,349,187 - Pf6N2E2_1983 0.47 -1.2 2,349,187 - Pf6N2E2_1983 0.47 +0.6 2,349,187 - Pf6N2E2_1983 0.47 -1.2 2,349,281 - -1.6 2,349,281 - +0.4 2,349,298 - -0.9 2,349,494 - Pf6N2E2_1984 0.30 -0.7 2,349,497 + Pf6N2E2_1984 0.30 -0.7 2,349,497 + -1.7 2,349,567 - Pf6N2E2_1984 0.40 +0.3 2,349,598 - Pf6N2E2_1984 0.44 +1.4 2,349,669 + Pf6N2E2_1984 0.54 -1.2 2,349,859 + Pf6N2E2_1984 0.80 -1.3 2,349,860 - Pf6N2E2_1984 0.80 -1.6 2,349,904 + Pf6N2E2_1984 0.87 -3.1 2,350,099 + -2.2 2,350,787 + -0.5 2,350,957 + +0.0
Or see this region's nucleotide sequence