Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1938 and Pf6N2E2_1939 are separated by 58 nucleotides Pf6N2E2_1939 and Pf6N2E2_1940 are separated by 323 nucleotides
Pf6N2E2_1938 - FIG00803671: hypothetical protein, at 2,297,185 to 2,298,123
_1938
Pf6N2E2_1939 - Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2), at 2,298,182 to 2,300,617
_1939
Pf6N2E2_1940 - TcuA: flavoprotein used to oxidize tricarballylate to cis-aconitate, at 2,300,941 to 2,302,395
_1940
Position (kb)
2298
2299
2300
2301 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2297.191 kb on + strand at 2297.310 kb on - strand, within Pf6N2E2_1938 at 2297.644 kb on + strand, within Pf6N2E2_1938 at 2297.644 kb on + strand, within Pf6N2E2_1938 at 2297.645 kb on - strand, within Pf6N2E2_1938 at 2297.648 kb on - strand, within Pf6N2E2_1938 at 2297.648 kb on - strand, within Pf6N2E2_1938 at 2297.794 kb on - strand, within Pf6N2E2_1938 at 2297.796 kb on + strand, within Pf6N2E2_1938 at 2297.796 kb on + strand, within Pf6N2E2_1938 at 2297.884 kb on + strand, within Pf6N2E2_1938 at 2297.884 kb on + strand, within Pf6N2E2_1938 at 2297.884 kb on + strand, within Pf6N2E2_1938 at 2297.884 kb on + strand, within Pf6N2E2_1938 at 2297.922 kb on + strand, within Pf6N2E2_1938 at 2298.028 kb on + strand, within Pf6N2E2_1938 at 2298.158 kb on + strand at 2298.159 kb on - strand at 2298.252 kb on + strand at 2298.253 kb on - strand at 2298.259 kb on + strand at 2298.259 kb on + strand at 2298.259 kb on + strand at 2298.273 kb on + strand at 2298.273 kb on + strand at 2298.273 kb on + strand at 2298.273 kb on + strand at 2298.273 kb on + strand at 2298.274 kb on - strand at 2298.274 kb on - strand at 2298.274 kb on - strand at 2298.274 kb on - strand at 2298.274 kb on - strand at 2298.342 kb on - strand at 2298.344 kb on + strand at 2298.345 kb on - strand at 2298.381 kb on + strand at 2298.382 kb on - strand at 2298.420 kb on - strand at 2298.420 kb on - strand at 2298.526 kb on - strand, within Pf6N2E2_1939 at 2298.807 kb on + strand, within Pf6N2E2_1939 at 2298.807 kb on + strand, within Pf6N2E2_1939 at 2298.808 kb on - strand, within Pf6N2E2_1939 at 2298.808 kb on - strand, within Pf6N2E2_1939 at 2299.040 kb on + strand, within Pf6N2E2_1939 at 2299.043 kb on + strand, within Pf6N2E2_1939 at 2299.043 kb on + strand, within Pf6N2E2_1939 at 2299.085 kb on + strand, within Pf6N2E2_1939 at 2299.086 kb on - strand, within Pf6N2E2_1939 at 2299.086 kb on - strand, within Pf6N2E2_1939 at 2299.139 kb on + strand, within Pf6N2E2_1939 at 2299.196 kb on + strand, within Pf6N2E2_1939 at 2299.197 kb on - strand, within Pf6N2E2_1939 at 2299.214 kb on + strand, within Pf6N2E2_1939 at 2299.215 kb on - strand, within Pf6N2E2_1939 at 2299.397 kb on + strand, within Pf6N2E2_1939 at 2299.397 kb on + strand, within Pf6N2E2_1939 at 2299.398 kb on - strand, within Pf6N2E2_1939 at 2299.400 kb on + strand, within Pf6N2E2_1939 at 2299.400 kb on + strand, within Pf6N2E2_1939 at 2299.401 kb on - strand, within Pf6N2E2_1939 at 2299.463 kb on + strand, within Pf6N2E2_1939 at 2299.464 kb on - strand, within Pf6N2E2_1939 at 2299.464 kb on - strand, within Pf6N2E2_1939 at 2299.464 kb on - strand, within Pf6N2E2_1939 at 2299.497 kb on - strand, within Pf6N2E2_1939 at 2299.518 kb on - strand, within Pf6N2E2_1939 at 2299.613 kb on + strand, within Pf6N2E2_1939 at 2299.614 kb on - strand, within Pf6N2E2_1939 at 2299.614 kb on - strand, within Pf6N2E2_1939 at 2299.780 kb on + strand, within Pf6N2E2_1939 at 2299.781 kb on - strand, within Pf6N2E2_1939 at 2299.781 kb on - strand, within Pf6N2E2_1939 at 2299.781 kb on - strand, within Pf6N2E2_1939 at 2300.152 kb on + strand, within Pf6N2E2_1939 at 2300.152 kb on + strand, within Pf6N2E2_1939 at 2300.153 kb on - strand, within Pf6N2E2_1939 at 2300.153 kb on - strand, within Pf6N2E2_1939 at 2300.391 kb on + strand at 2300.392 kb on - strand at 2300.392 kb on - strand at 2300.416 kb on + strand at 2300.416 kb on + strand at 2300.416 kb on + strand at 2300.417 kb on - strand at 2300.417 kb on - strand at 2300.417 kb on - strand at 2300.417 kb on - strand at 2300.417 kb on - strand at 2300.417 kb on - strand at 2300.417 kb on - strand at 2300.502 kb on - strand at 2300.644 kb on - strand at 2300.716 kb on + strand at 2300.716 kb on + strand at 2300.716 kb on + strand at 2300.717 kb on - strand at 2300.717 kb on - strand at 2300.717 kb on - strand at 2300.717 kb on - strand at 2300.717 kb on - strand at 2300.717 kb on - strand at 2300.717 kb on - strand at 2300.717 kb on - strand at 2300.719 kb on - strand at 2300.751 kb on - strand at 2300.768 kb on - strand at 2300.768 kb on - strand at 2300.798 kb on + strand at 2300.829 kb on + strand at 2300.830 kb on - strand at 2300.883 kb on + strand at 2300.884 kb on - strand at 2300.884 kb on - strand at 2300.937 kb on + strand at 2300.938 kb on - strand at 2300.938 kb on - strand at 2300.938 kb on - strand at 2300.960 kb on + strand at 2300.960 kb on + strand at 2300.961 kb on - strand at 2300.981 kb on + strand at 2300.982 kb on - strand at 2300.982 kb on - strand at 2301.127 kb on + strand, within Pf6N2E2_1940 at 2301.143 kb on - strand at 2301.177 kb on + strand, within Pf6N2E2_1940 at 2301.177 kb on + strand, within Pf6N2E2_1940 at 2301.401 kb on + strand, within Pf6N2E2_1940 at 2301.401 kb on + strand, within Pf6N2E2_1940 at 2301.402 kb on - strand, within Pf6N2E2_1940 at 2301.452 kb on + strand, within Pf6N2E2_1940 at 2301.452 kb on + strand, within Pf6N2E2_1940 at 2301.452 kb on + strand, within Pf6N2E2_1940 at 2301.453 kb on - strand, within Pf6N2E2_1940
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,297,191 + -0.4 2,297,310 - Pf6N2E2_1938 0.13 -1.5 2,297,644 + Pf6N2E2_1938 0.49 -0.1 2,297,644 + Pf6N2E2_1938 0.49 -0.4 2,297,645 - Pf6N2E2_1938 0.49 -0.5 2,297,648 - Pf6N2E2_1938 0.49 -0.5 2,297,648 - Pf6N2E2_1938 0.49 +0.3 2,297,794 - Pf6N2E2_1938 0.65 -0.6 2,297,796 + Pf6N2E2_1938 0.65 +0.4 2,297,796 + Pf6N2E2_1938 0.65 -0.3 2,297,884 + Pf6N2E2_1938 0.74 +0.0 2,297,884 + Pf6N2E2_1938 0.74 +0.4 2,297,884 + Pf6N2E2_1938 0.74 -0.9 2,297,884 + Pf6N2E2_1938 0.74 -0.2 2,297,922 + Pf6N2E2_1938 0.78 -0.0 2,298,028 + Pf6N2E2_1938 0.90 +0.6 2,298,158 + +0.4 2,298,159 - +0.2 2,298,252 + +0.3 2,298,253 - -1.6 2,298,259 + +0.8 2,298,259 + -1.1 2,298,259 + -0.3 2,298,273 + +0.2 2,298,273 + -0.7 2,298,273 + +0.4 2,298,273 + +0.6 2,298,273 + -0.1 2,298,274 - +0.2 2,298,274 - +1.6 2,298,274 - +0.2 2,298,274 - -0.6 2,298,274 - +0.4 2,298,342 - -0.5 2,298,344 + -0.1 2,298,345 - -1.1 2,298,381 + -1.3 2,298,382 - +0.1 2,298,420 - +0.6 2,298,420 - -0.6 2,298,526 - Pf6N2E2_1939 0.14 -2.7 2,298,807 + Pf6N2E2_1939 0.26 +1.9 2,298,807 + Pf6N2E2_1939 0.26 +0.7 2,298,808 - Pf6N2E2_1939 0.26 +0.1 2,298,808 - Pf6N2E2_1939 0.26 -0.3 2,299,040 + Pf6N2E2_1939 0.35 +0.9 2,299,043 + Pf6N2E2_1939 0.35 +0.4 2,299,043 + Pf6N2E2_1939 0.35 -0.1 2,299,085 + Pf6N2E2_1939 0.37 +0.5 2,299,086 - Pf6N2E2_1939 0.37 -1.7 2,299,086 - Pf6N2E2_1939 0.37 -0.0 2,299,139 + Pf6N2E2_1939 0.39 -0.1 2,299,196 + Pf6N2E2_1939 0.42 -0.4 2,299,197 - Pf6N2E2_1939 0.42 +0.8 2,299,214 + Pf6N2E2_1939 0.42 -0.7 2,299,215 - Pf6N2E2_1939 0.42 +0.9 2,299,397 + Pf6N2E2_1939 0.50 -0.0 2,299,397 + Pf6N2E2_1939 0.50 +0.1 2,299,398 - Pf6N2E2_1939 0.50 +1.1 2,299,400 + Pf6N2E2_1939 0.50 +0.7 2,299,400 + Pf6N2E2_1939 0.50 +1.0 2,299,401 - Pf6N2E2_1939 0.50 +1.3 2,299,463 + Pf6N2E2_1939 0.53 +1.3 2,299,464 - Pf6N2E2_1939 0.53 +0.3 2,299,464 - Pf6N2E2_1939 0.53 -0.1 2,299,464 - Pf6N2E2_1939 0.53 -0.7 2,299,497 - Pf6N2E2_1939 0.54 +1.4 2,299,518 - Pf6N2E2_1939 0.55 +1.0 2,299,613 + Pf6N2E2_1939 0.59 +1.0 2,299,614 - Pf6N2E2_1939 0.59 +0.4 2,299,614 - Pf6N2E2_1939 0.59 +0.2 2,299,780 + Pf6N2E2_1939 0.66 +0.2 2,299,781 - Pf6N2E2_1939 0.66 -0.1 2,299,781 - Pf6N2E2_1939 0.66 -0.1 2,299,781 - Pf6N2E2_1939 0.66 +1.0 2,300,152 + Pf6N2E2_1939 0.81 +1.0 2,300,152 + Pf6N2E2_1939 0.81 -0.7 2,300,153 - Pf6N2E2_1939 0.81 +0.8 2,300,153 - Pf6N2E2_1939 0.81 -0.1 2,300,391 + +1.2 2,300,392 - +0.0 2,300,392 - -0.6 2,300,416 + +0.4 2,300,416 + +0.4 2,300,416 + +0.3 2,300,417 - +1.0 2,300,417 - +0.5 2,300,417 - -0.1 2,300,417 - -2.7 2,300,417 - +1.3 2,300,417 - +0.6 2,300,417 - -0.7 2,300,502 - -0.6 2,300,644 - +0.8 2,300,716 + -0.1 2,300,716 + -0.0 2,300,716 + -0.4 2,300,717 - -0.7 2,300,717 - +0.4 2,300,717 - -0.5 2,300,717 - +0.9 2,300,717 - +0.9 2,300,717 - -0.7 2,300,717 - -0.0 2,300,717 - +0.4 2,300,719 - -0.9 2,300,751 - +0.9 2,300,768 - +0.6 2,300,768 - +0.7 2,300,798 + -0.6 2,300,829 + +1.3 2,300,830 - +0.3 2,300,883 + -0.6 2,300,884 - +0.9 2,300,884 - -0.5 2,300,937 + -0.1 2,300,938 - -0.1 2,300,938 - +1.3 2,300,938 - -0.9 2,300,960 + +1.7 2,300,960 + +0.4 2,300,961 - +0.4 2,300,981 + -0.8 2,300,982 - +1.1 2,300,982 - +0.3 2,301,127 + Pf6N2E2_1940 0.13 -3.3 2,301,143 - +1.4 2,301,177 + Pf6N2E2_1940 0.16 -1.5 2,301,177 + Pf6N2E2_1940 0.16 -0.6 2,301,401 + Pf6N2E2_1940 0.32 +0.0 2,301,401 + Pf6N2E2_1940 0.32 -0.0 2,301,402 - Pf6N2E2_1940 0.32 -1.7 2,301,452 + Pf6N2E2_1940 0.35 +0.3 2,301,452 + Pf6N2E2_1940 0.35 -1.7 2,301,452 + Pf6N2E2_1940 0.35 -0.1 2,301,453 - Pf6N2E2_1940 0.35 +0.9
Or see this region's nucleotide sequence