Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1739 and Pf6N2E2_1740 overlap by 11 nucleotides Pf6N2E2_1740 and Pf6N2E2_1741 overlap by 4 nucleotides
Pf6N2E2_1739 - Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8), at 2,051,415 to 2,052,698
_1739
Pf6N2E2_1740 - Transketolase, C-terminal section (EC 2.2.1.1), at 2,052,688 to 2,053,698
_1740
Pf6N2E2_1741 - Transketolase, N-terminal section (EC 2.2.1.1), at 2,053,695 to 2,054,579
_1741
Position (kb)
2052
2053
2054 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2051.706 kb on - strand, within Pf6N2E2_1739 at 2051.706 kb on - strand, within Pf6N2E2_1739 at 2051.707 kb on + strand, within Pf6N2E2_1739 at 2051.707 kb on + strand, within Pf6N2E2_1739 at 2051.707 kb on + strand, within Pf6N2E2_1739 at 2051.707 kb on + strand, within Pf6N2E2_1739 at 2051.708 kb on - strand, within Pf6N2E2_1739 at 2051.734 kb on + strand, within Pf6N2E2_1739 at 2051.735 kb on - strand, within Pf6N2E2_1739 at 2052.161 kb on - strand, within Pf6N2E2_1739 at 2052.265 kb on + strand, within Pf6N2E2_1739 at 2052.266 kb on - strand, within Pf6N2E2_1739 at 2052.266 kb on - strand, within Pf6N2E2_1739 at 2052.584 kb on - strand at 2052.782 kb on + strand at 2052.782 kb on + strand at 2052.845 kb on - strand, within Pf6N2E2_1740 at 2052.845 kb on - strand, within Pf6N2E2_1740 at 2053.313 kb on + strand, within Pf6N2E2_1740 at 2053.313 kb on + strand, within Pf6N2E2_1740 at 2053.371 kb on - strand, within Pf6N2E2_1740 at 2053.629 kb on - strand at 2053.629 kb on - strand at 2053.665 kb on - strand at 2053.792 kb on + strand, within Pf6N2E2_1741 at 2053.793 kb on - strand, within Pf6N2E2_1741 at 2054.107 kb on + strand, within Pf6N2E2_1741 at 2054.107 kb on + strand, within Pf6N2E2_1741 at 2054.118 kb on + strand, within Pf6N2E2_1741 at 2054.119 kb on - strand, within Pf6N2E2_1741 at 2054.399 kb on - strand, within Pf6N2E2_1741 at 2054.399 kb on - strand, within Pf6N2E2_1741 at 2054.399 kb on - strand, within Pf6N2E2_1741 at 2054.401 kb on + strand, within Pf6N2E2_1741 at 2054.444 kb on - strand, within Pf6N2E2_1741
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,051,706 - Pf6N2E2_1739 0.23 -0.5 2,051,706 - Pf6N2E2_1739 0.23 -0.6 2,051,707 + Pf6N2E2_1739 0.23 -0.2 2,051,707 + Pf6N2E2_1739 0.23 -1.1 2,051,707 + Pf6N2E2_1739 0.23 +0.1 2,051,707 + Pf6N2E2_1739 0.23 -0.7 2,051,708 - Pf6N2E2_1739 0.23 +1.3 2,051,734 + Pf6N2E2_1739 0.25 -0.5 2,051,735 - Pf6N2E2_1739 0.25 +0.1 2,052,161 - Pf6N2E2_1739 0.58 +0.2 2,052,265 + Pf6N2E2_1739 0.66 -0.7 2,052,266 - Pf6N2E2_1739 0.66 -0.8 2,052,266 - Pf6N2E2_1739 0.66 -0.1 2,052,584 - +0.8 2,052,782 + +0.2 2,052,782 + +1.2 2,052,845 - Pf6N2E2_1740 0.16 -0.5 2,052,845 - Pf6N2E2_1740 0.16 +1.9 2,053,313 + Pf6N2E2_1740 0.62 +0.1 2,053,313 + Pf6N2E2_1740 0.62 +0.3 2,053,371 - Pf6N2E2_1740 0.68 -0.5 2,053,629 - +0.1 2,053,629 - +1.2 2,053,665 - +0.6 2,053,792 + Pf6N2E2_1741 0.11 -2.8 2,053,793 - Pf6N2E2_1741 0.11 -0.1 2,054,107 + Pf6N2E2_1741 0.47 -0.2 2,054,107 + Pf6N2E2_1741 0.47 -0.1 2,054,118 + Pf6N2E2_1741 0.48 +0.6 2,054,119 - Pf6N2E2_1741 0.48 -0.5 2,054,399 - Pf6N2E2_1741 0.80 +0.4 2,054,399 - Pf6N2E2_1741 0.80 +0.3 2,054,399 - Pf6N2E2_1741 0.80 -0.5 2,054,401 + Pf6N2E2_1741 0.80 -1.2 2,054,444 - Pf6N2E2_1741 0.85 +2.7
Or see this region's nucleotide sequence