Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1675 and Pf6N2E2_1676 are separated by 10 nucleotides Pf6N2E2_1676 and Pf6N2E2_1677 are separated by 157 nucleotides
Pf6N2E2_1675 - Lipid carrier protein or keto acyl-CoA thiolase Ltp3 (EC 2.3.1.16), at 1,981,221 to 1,982,417
_1675
Pf6N2E2_1676 - conserved protein associated with acetyl-CoA C-acyltransferase, at 1,982,428 to 1,982,868
_1676
Pf6N2E2_1677 - Chalcone synthase (EC 2.3.1.74), at 1,983,026 to 1,984,075
_1677
Position (kb)
1982
1983 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1981.472 kb on + strand, within Pf6N2E2_1675 at 1981.473 kb on - strand, within Pf6N2E2_1675 at 1981.539 kb on + strand, within Pf6N2E2_1675 at 1981.539 kb on + strand, within Pf6N2E2_1675 at 1981.599 kb on + strand at 1981.599 kb on + strand, within Pf6N2E2_1675 at 1981.600 kb on - strand at 1981.632 kb on + strand, within Pf6N2E2_1675 at 1981.632 kb on + strand, within Pf6N2E2_1675 at 1981.723 kb on + strand, within Pf6N2E2_1675 at 1981.751 kb on + strand, within Pf6N2E2_1675 at 1981.751 kb on + strand at 1981.751 kb on + strand, within Pf6N2E2_1675 at 1981.751 kb on + strand, within Pf6N2E2_1675 at 1981.751 kb on + strand, within Pf6N2E2_1675 at 1981.755 kb on + strand, within Pf6N2E2_1675 at 1981.755 kb on + strand, within Pf6N2E2_1675 at 1981.921 kb on + strand, within Pf6N2E2_1675 at 1981.941 kb on + strand, within Pf6N2E2_1675 at 1981.942 kb on - strand, within Pf6N2E2_1675 at 1981.974 kb on + strand, within Pf6N2E2_1675 at 1982.017 kb on + strand, within Pf6N2E2_1675 at 1982.017 kb on + strand, within Pf6N2E2_1675 at 1982.017 kb on + strand at 1982.017 kb on + strand, within Pf6N2E2_1675 at 1982.017 kb on + strand, within Pf6N2E2_1675 at 1982.017 kb on + strand, within Pf6N2E2_1675 at 1982.017 kb on + strand, within Pf6N2E2_1675 at 1982.018 kb on - strand, within Pf6N2E2_1675 at 1982.064 kb on + strand, within Pf6N2E2_1675 at 1982.158 kb on + strand, within Pf6N2E2_1675 at 1982.158 kb on + strand, within Pf6N2E2_1675 at 1982.158 kb on + strand, within Pf6N2E2_1675 at 1982.159 kb on - strand, within Pf6N2E2_1675 at 1982.203 kb on + strand, within Pf6N2E2_1675 at 1982.203 kb on + strand, within Pf6N2E2_1675 at 1982.203 kb on + strand, within Pf6N2E2_1675 at 1982.291 kb on + strand, within Pf6N2E2_1675 at 1982.674 kb on + strand, within Pf6N2E2_1676 at 1982.747 kb on + strand, within Pf6N2E2_1676 at 1982.747 kb on + strand, within Pf6N2E2_1676 at 1982.747 kb on + strand, within Pf6N2E2_1676 at 1982.845 kb on + strand at 1982.845 kb on + strand at 1982.845 kb on + strand at 1982.846 kb on - strand at 1982.855 kb on + strand at 1982.866 kb on + strand at 1982.995 kb on - strand at 1983.010 kb on + strand at 1983.032 kb on - strand at 1983.065 kb on + strand at 1983.066 kb on - strand at 1983.066 kb on - strand at 1983.191 kb on - strand, within Pf6N2E2_1677 at 1983.411 kb on + strand, within Pf6N2E2_1677 at 1983.665 kb on + strand, within Pf6N2E2_1677 at 1983.665 kb on + strand, within Pf6N2E2_1677 at 1983.689 kb on + strand, within Pf6N2E2_1677 at 1983.689 kb on + strand, within Pf6N2E2_1677 at 1983.689 kb on + strand, within Pf6N2E2_1677 at 1983.690 kb on - strand, within Pf6N2E2_1677 at 1983.690 kb on - strand, within Pf6N2E2_1677 at 1983.690 kb on - strand, within Pf6N2E2_1677 at 1983.698 kb on + strand, within Pf6N2E2_1677 at 1983.699 kb on - strand, within Pf6N2E2_1677 at 1983.699 kb on - strand, within Pf6N2E2_1677 at 1983.792 kb on - strand, within Pf6N2E2_1677
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 1,981,472 + Pf6N2E2_1675 0.21 -1.8 1,981,473 - Pf6N2E2_1675 0.21 -0.8 1,981,539 + Pf6N2E2_1675 0.27 +1.9 1,981,539 + Pf6N2E2_1675 0.27 +0.2 1,981,599 + +1.3 1,981,599 + Pf6N2E2_1675 0.32 +0.1 1,981,600 - +0.8 1,981,632 + Pf6N2E2_1675 0.34 +0.3 1,981,632 + Pf6N2E2_1675 0.34 -0.9 1,981,723 + Pf6N2E2_1675 0.42 -0.2 1,981,751 + Pf6N2E2_1675 0.44 +1.0 1,981,751 + +1.8 1,981,751 + Pf6N2E2_1675 0.44 +0.7 1,981,751 + Pf6N2E2_1675 0.44 +0.6 1,981,751 + Pf6N2E2_1675 0.44 +2.0 1,981,755 + Pf6N2E2_1675 0.45 +1.3 1,981,755 + Pf6N2E2_1675 0.45 +0.9 1,981,921 + Pf6N2E2_1675 0.58 +0.5 1,981,941 + Pf6N2E2_1675 0.60 +1.1 1,981,942 - Pf6N2E2_1675 0.60 -0.2 1,981,974 + Pf6N2E2_1675 0.63 +0.1 1,982,017 + Pf6N2E2_1675 0.66 +3.5 1,982,017 + Pf6N2E2_1675 0.66 -0.2 1,982,017 + -0.2 1,982,017 + Pf6N2E2_1675 0.66 +1.5 1,982,017 + Pf6N2E2_1675 0.66 +1.1 1,982,017 + Pf6N2E2_1675 0.66 +0.1 1,982,017 + Pf6N2E2_1675 0.66 +2.9 1,982,018 - Pf6N2E2_1675 0.67 -0.7 1,982,064 + Pf6N2E2_1675 0.70 +0.5 1,982,158 + Pf6N2E2_1675 0.78 +1.6 1,982,158 + Pf6N2E2_1675 0.78 +0.3 1,982,158 + Pf6N2E2_1675 0.78 +1.1 1,982,159 - Pf6N2E2_1675 0.78 -0.8 1,982,203 + Pf6N2E2_1675 0.82 +0.2 1,982,203 + Pf6N2E2_1675 0.82 +1.3 1,982,203 + Pf6N2E2_1675 0.82 +0.2 1,982,291 + Pf6N2E2_1675 0.89 +1.3 1,982,674 + Pf6N2E2_1676 0.56 -0.8 1,982,747 + Pf6N2E2_1676 0.72 -0.5 1,982,747 + Pf6N2E2_1676 0.72 -1.0 1,982,747 + Pf6N2E2_1676 0.72 -0.8 1,982,845 + -0.6 1,982,845 + +1.1 1,982,845 + -0.9 1,982,846 - -2.1 1,982,855 + +0.3 1,982,866 + -0.4 1,982,995 - -1.2 1,983,010 + +1.4 1,983,032 - +0.1 1,983,065 + +1.9 1,983,066 - +1.1 1,983,066 - -0.2 1,983,191 - Pf6N2E2_1677 0.16 +1.6 1,983,411 + Pf6N2E2_1677 0.37 -1.6 1,983,665 + Pf6N2E2_1677 0.61 -0.7 1,983,665 + Pf6N2E2_1677 0.61 +1.6 1,983,689 + Pf6N2E2_1677 0.63 +0.1 1,983,689 + Pf6N2E2_1677 0.63 -0.1 1,983,689 + Pf6N2E2_1677 0.63 +0.3 1,983,690 - Pf6N2E2_1677 0.63 -1.2 1,983,690 - Pf6N2E2_1677 0.63 +1.8 1,983,690 - Pf6N2E2_1677 0.63 +1.6 1,983,698 + Pf6N2E2_1677 0.64 -0.0 1,983,699 - Pf6N2E2_1677 0.64 +1.9 1,983,699 - Pf6N2E2_1677 0.64 +0.6 1,983,792 - Pf6N2E2_1677 0.73 +0.8
Or see this region's nucleotide sequence