Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1600

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1598 and Pf6N2E2_1599 are separated by 9 nucleotidesPf6N2E2_1599 and Pf6N2E2_1600 are separated by 130 nucleotidesPf6N2E2_1600 and Pf6N2E2_1601 are separated by 5 nucleotidesPf6N2E2_1601 and Pf6N2E2_1602 are separated by 70 nucleotides Pf6N2E2_1598 - Nitrate reductase cytochrome c550-type subunit, at 1,870,998 to 1,871,483 _1598 Pf6N2E2_1599 - Cytochrome c-type protein NapC, at 1,871,493 to 1,872,089 _1599 Pf6N2E2_1600 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 1,872,220 to 1,873,872 _1600 Pf6N2E2_1601 - DNA-binding response regulator, LuxR family, at 1,873,878 to 1,874,459 _1601 Pf6N2E2_1602 - hypothetical protein, at 1,874,530 to 1,875,312 _1602 Position (kb) 1872 1873 1874Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1871.319 kb on + strand, within Pf6N2E2_1598at 1871.320 kb on - strand, within Pf6N2E2_1598at 1871.368 kb on - strand, within Pf6N2E2_1598at 1871.404 kb on + strand, within Pf6N2E2_1598at 1871.404 kb on + strand, within Pf6N2E2_1598at 1871.405 kb on - strand, within Pf6N2E2_1598at 1871.405 kb on - strand, within Pf6N2E2_1598at 1871.524 kb on - strandat 1871.553 kb on + strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.557 kb on - strand, within Pf6N2E2_1599at 1871.557 kb on - strand, within Pf6N2E2_1599at 1871.715 kb on + strand, within Pf6N2E2_1599at 1871.781 kb on - strand, within Pf6N2E2_1599at 1872.224 kb on - strandat 1872.224 kb on - strandat 1872.224 kb on - strandat 1872.224 kb on - strandat 1872.370 kb on + strandat 1872.370 kb on + strandat 1872.524 kb on - strand, within Pf6N2E2_1600at 1872.524 kb on - strand, within Pf6N2E2_1600at 1872.982 kb on + strand, within Pf6N2E2_1600at 1873.103 kb on + strand, within Pf6N2E2_1600at 1873.103 kb on + strand, within Pf6N2E2_1600at 1873.104 kb on - strand, within Pf6N2E2_1600at 1873.229 kb on + strand, within Pf6N2E2_1600at 1873.397 kb on - strand, within Pf6N2E2_1600at 1873.514 kb on + strand, within Pf6N2E2_1600at 1873.595 kb on + strand, within Pf6N2E2_1600at 1873.595 kb on + strand, within Pf6N2E2_1600at 1873.596 kb on - strand, within Pf6N2E2_1600at 1873.889 kb on + strandat 1873.890 kb on - strandat 1874.285 kb on + strand, within Pf6N2E2_1601at 1874.340 kb on + strand, within Pf6N2E2_1601at 1874.340 kb on + strand, within Pf6N2E2_1601at 1874.340 kb on + strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.341 kb on - strand, within Pf6N2E2_1601at 1874.343 kb on - strand, within Pf6N2E2_1601at 1874.391 kb on + strand, within Pf6N2E2_1601at 1874.391 kb on + strand, within Pf6N2E2_1601at 1874.392 kb on - strand, within Pf6N2E2_1601at 1874.508 kb on - strandat 1874.543 kb on + strandat 1874.543 kb on + strandat 1874.543 kb on + strandat 1874.766 kb on + strand, within Pf6N2E2_1602at 1874.766 kb on + strand, within Pf6N2E2_1602at 1874.768 kb on + strand, within Pf6N2E2_1602at 1874.768 kb on + strand, within Pf6N2E2_1602at 1874.831 kb on + strand, within Pf6N2E2_1602

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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1,871,319 + Pf6N2E2_1598 0.66 +1.2
1,871,320 - Pf6N2E2_1598 0.66 -1.5
1,871,368 - Pf6N2E2_1598 0.76 -0.3
1,871,404 + Pf6N2E2_1598 0.84 +0.9
1,871,404 + Pf6N2E2_1598 0.84 -1.0
1,871,405 - Pf6N2E2_1598 0.84 +0.1
1,871,405 - Pf6N2E2_1598 0.84 -0.1
1,871,524 - -0.3
1,871,553 + Pf6N2E2_1599 0.10 -1.3
1,871,554 - Pf6N2E2_1599 0.10 -2.1
1,871,554 - Pf6N2E2_1599 0.10 -0.8
1,871,554 - Pf6N2E2_1599 0.10 -0.3
1,871,554 - Pf6N2E2_1599 0.10 +0.3
1,871,554 - Pf6N2E2_1599 0.10 -0.4
1,871,554 - Pf6N2E2_1599 0.10 -0.1
1,871,554 - Pf6N2E2_1599 0.10 -0.3
1,871,557 - Pf6N2E2_1599 0.11 +1.3
1,871,557 - Pf6N2E2_1599 0.11 -0.6
1,871,715 + Pf6N2E2_1599 0.37 +0.8
1,871,781 - Pf6N2E2_1599 0.48 +1.5
1,872,224 - +0.6
1,872,224 - -1.4
1,872,224 - -1.3
1,872,224 - +1.3
1,872,370 + -0.6
1,872,370 + +0.5
1,872,524 - Pf6N2E2_1600 0.18 -0.5
1,872,524 - Pf6N2E2_1600 0.18 +0.6
1,872,982 + Pf6N2E2_1600 0.46 +1.2
1,873,103 + Pf6N2E2_1600 0.53 -0.2
1,873,103 + Pf6N2E2_1600 0.53 +0.2
1,873,104 - Pf6N2E2_1600 0.53 -0.8
1,873,229 + Pf6N2E2_1600 0.61 +0.1
1,873,397 - Pf6N2E2_1600 0.71 -1.0
1,873,514 + Pf6N2E2_1600 0.78 -1.6
1,873,595 + Pf6N2E2_1600 0.83 +0.4
1,873,595 + Pf6N2E2_1600 0.83 +0.3
1,873,596 - Pf6N2E2_1600 0.83 -0.9
1,873,889 + -0.3
1,873,890 - +0.7
1,874,285 + Pf6N2E2_1601 0.70 -1.0
1,874,340 + Pf6N2E2_1601 0.79 +0.3
1,874,340 + Pf6N2E2_1601 0.79 -0.7
1,874,340 + Pf6N2E2_1601 0.79 -0.6
1,874,341 - Pf6N2E2_1601 0.80 +0.4
1,874,341 - Pf6N2E2_1601 0.80 -0.4
1,874,341 - Pf6N2E2_1601 0.80 -1.5
1,874,341 - Pf6N2E2_1601 0.80 +2.5
1,874,341 - Pf6N2E2_1601 0.80 +1.6
1,874,341 - Pf6N2E2_1601 0.80 -0.5
1,874,341 - Pf6N2E2_1601 0.80 +2.4
1,874,341 - Pf6N2E2_1601 0.80 +0.9
1,874,341 - Pf6N2E2_1601 0.80 -0.9
1,874,343 - Pf6N2E2_1601 0.80 +1.0
1,874,391 + Pf6N2E2_1601 0.88 +0.0
1,874,391 + Pf6N2E2_1601 0.88 -0.9
1,874,392 - Pf6N2E2_1601 0.88 +0.6
1,874,508 - -0.7
1,874,543 + -1.2
1,874,543 + +0.1
1,874,543 + -0.4
1,874,766 + Pf6N2E2_1602 0.30 -1.3
1,874,766 + Pf6N2E2_1602 0.30 -0.3
1,874,768 + Pf6N2E2_1602 0.30 +3.0
1,874,768 + Pf6N2E2_1602 0.30 +0.2
1,874,831 + Pf6N2E2_1602 0.38 +0.3

Or see this region's nucleotide sequence