Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1426 and Pf6N2E2_1427 are separated by 118 nucleotides Pf6N2E2_1427 and Pf6N2E2_1428 are separated by 305 nucleotides
Pf6N2E2_1426 - FIG00966546: hypothetical protein, at 1,674,221 to 1,675,558
_1426
Pf6N2E2_1427 - 6-aminohexanoate-dimer hydrolase (EC 3.5.1.46), at 1,675,677 to 1,676,939
_1427
Pf6N2E2_1428 - Alkanesulfonates-binding protein, at 1,677,245 to 1,678,348
_1428
Position (kb)
1675
1676
1677 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1674.819 kb on + strand, within Pf6N2E2_1426 at 1674.819 kb on + strand, within Pf6N2E2_1426 at 1674.820 kb on - strand, within Pf6N2E2_1426 at 1674.820 kb on - strand, within Pf6N2E2_1426 at 1674.820 kb on - strand, within Pf6N2E2_1426 at 1674.924 kb on + strand, within Pf6N2E2_1426 at 1674.924 kb on + strand, within Pf6N2E2_1426 at 1674.925 kb on - strand, within Pf6N2E2_1426 at 1674.925 kb on - strand, within Pf6N2E2_1426 at 1674.925 kb on - strand, within Pf6N2E2_1426 at 1675.125 kb on + strand, within Pf6N2E2_1426 at 1675.125 kb on + strand, within Pf6N2E2_1426 at 1675.125 kb on + strand, within Pf6N2E2_1426 at 1675.126 kb on - strand, within Pf6N2E2_1426 at 1675.126 kb on - strand, within Pf6N2E2_1426 at 1675.176 kb on + strand, within Pf6N2E2_1426 at 1675.176 kb on + strand, within Pf6N2E2_1426 at 1675.177 kb on - strand, within Pf6N2E2_1426 at 1675.302 kb on + strand, within Pf6N2E2_1426 at 1675.329 kb on + strand, within Pf6N2E2_1426 at 1675.329 kb on + strand, within Pf6N2E2_1426 at 1675.330 kb on - strand, within Pf6N2E2_1426 at 1675.559 kb on - strand at 1675.577 kb on + strand at 1675.577 kb on + strand at 1675.578 kb on - strand at 1675.587 kb on - strand at 1675.596 kb on + strand at 1675.666 kb on - strand at 1675.804 kb on + strand, within Pf6N2E2_1427 at 1675.805 kb on - strand, within Pf6N2E2_1427 at 1675.858 kb on + strand, within Pf6N2E2_1427 at 1675.918 kb on + strand, within Pf6N2E2_1427 at 1675.918 kb on + strand, within Pf6N2E2_1427 at 1675.918 kb on + strand, within Pf6N2E2_1427 at 1676.207 kb on - strand, within Pf6N2E2_1427 at 1676.260 kb on + strand, within Pf6N2E2_1427 at 1676.261 kb on - strand, within Pf6N2E2_1427 at 1676.440 kb on + strand, within Pf6N2E2_1427 at 1676.440 kb on + strand, within Pf6N2E2_1427 at 1676.440 kb on + strand, within Pf6N2E2_1427 at 1676.441 kb on - strand, within Pf6N2E2_1427 at 1676.441 kb on - strand, within Pf6N2E2_1427 at 1677.202 kb on + strand at 1677.255 kb on + strand at 1677.430 kb on - strand, within Pf6N2E2_1428 at 1677.430 kb on - strand, within Pf6N2E2_1428 at 1677.430 kb on - strand, within Pf6N2E2_1428 at 1677.430 kb on - strand, within Pf6N2E2_1428 at 1677.708 kb on + strand, within Pf6N2E2_1428 at 1677.708 kb on + strand, within Pf6N2E2_1428 at 1677.709 kb on - strand, within Pf6N2E2_1428 at 1677.709 kb on - strand, within Pf6N2E2_1428 at 1677.709 kb on - strand, within Pf6N2E2_1428 at 1677.709 kb on - strand, within Pf6N2E2_1428 at 1677.732 kb on + strand, within Pf6N2E2_1428 at 1677.732 kb on + strand, within Pf6N2E2_1428 at 1677.732 kb on + strand, within Pf6N2E2_1428 at 1677.733 kb on - strand, within Pf6N2E2_1428 at 1677.733 kb on - strand, within Pf6N2E2_1428 at 1677.733 kb on - strand, within Pf6N2E2_1428 at 1677.780 kb on - strand, within Pf6N2E2_1428 at 1677.780 kb on - strand, within Pf6N2E2_1428 at 1677.853 kb on - strand, within Pf6N2E2_1428 at 1677.873 kb on - strand, within Pf6N2E2_1428
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 1,674,819 + Pf6N2E2_1426 0.45 +1.7 1,674,819 + Pf6N2E2_1426 0.45 -1.2 1,674,820 - Pf6N2E2_1426 0.45 +1.4 1,674,820 - Pf6N2E2_1426 0.45 +0.6 1,674,820 - Pf6N2E2_1426 0.45 -0.2 1,674,924 + Pf6N2E2_1426 0.53 +0.0 1,674,924 + Pf6N2E2_1426 0.53 +0.2 1,674,925 - Pf6N2E2_1426 0.53 +0.5 1,674,925 - Pf6N2E2_1426 0.53 +0.2 1,674,925 - Pf6N2E2_1426 0.53 -0.1 1,675,125 + Pf6N2E2_1426 0.68 +0.6 1,675,125 + Pf6N2E2_1426 0.68 -0.1 1,675,125 + Pf6N2E2_1426 0.68 -1.5 1,675,126 - Pf6N2E2_1426 0.68 +0.4 1,675,126 - Pf6N2E2_1426 0.68 +0.4 1,675,176 + Pf6N2E2_1426 0.71 +0.7 1,675,176 + Pf6N2E2_1426 0.71 +1.2 1,675,177 - Pf6N2E2_1426 0.71 +0.7 1,675,302 + Pf6N2E2_1426 0.81 -0.9 1,675,329 + Pf6N2E2_1426 0.83 +0.7 1,675,329 + Pf6N2E2_1426 0.83 +2.0 1,675,330 - Pf6N2E2_1426 0.83 +0.3 1,675,559 - +0.4 1,675,577 + +1.7 1,675,577 + +0.4 1,675,578 - +0.6 1,675,587 - +0.8 1,675,596 + -1.0 1,675,666 - +0.5 1,675,804 + Pf6N2E2_1427 0.10 +1.7 1,675,805 - Pf6N2E2_1427 0.10 +1.4 1,675,858 + Pf6N2E2_1427 0.14 +0.7 1,675,918 + Pf6N2E2_1427 0.19 +0.6 1,675,918 + Pf6N2E2_1427 0.19 +0.2 1,675,918 + Pf6N2E2_1427 0.19 +0.7 1,676,207 - Pf6N2E2_1427 0.42 +2.2 1,676,260 + Pf6N2E2_1427 0.46 +1.3 1,676,261 - Pf6N2E2_1427 0.46 +1.2 1,676,440 + Pf6N2E2_1427 0.60 -1.3 1,676,440 + Pf6N2E2_1427 0.60 +0.3 1,676,440 + Pf6N2E2_1427 0.60 +0.7 1,676,441 - Pf6N2E2_1427 0.60 -0.6 1,676,441 - Pf6N2E2_1427 0.60 +0.1 1,677,202 + -1.3 1,677,255 + -1.3 1,677,430 - Pf6N2E2_1428 0.17 +1.1 1,677,430 - Pf6N2E2_1428 0.17 +0.5 1,677,430 - Pf6N2E2_1428 0.17 -0.5 1,677,430 - Pf6N2E2_1428 0.17 -0.1 1,677,708 + Pf6N2E2_1428 0.42 -3.0 1,677,708 + Pf6N2E2_1428 0.42 -1.1 1,677,709 - Pf6N2E2_1428 0.42 -2.3 1,677,709 - Pf6N2E2_1428 0.42 +0.7 1,677,709 - Pf6N2E2_1428 0.42 +0.3 1,677,709 - Pf6N2E2_1428 0.42 +0.7 1,677,732 + Pf6N2E2_1428 0.44 -0.8 1,677,732 + Pf6N2E2_1428 0.44 +0.0 1,677,732 + Pf6N2E2_1428 0.44 +0.8 1,677,733 - Pf6N2E2_1428 0.44 +0.3 1,677,733 - Pf6N2E2_1428 0.44 +0.4 1,677,733 - Pf6N2E2_1428 0.44 +0.5 1,677,780 - Pf6N2E2_1428 0.48 +1.4 1,677,780 - Pf6N2E2_1428 0.48 -0.6 1,677,853 - Pf6N2E2_1428 0.55 +0.1 1,677,873 - Pf6N2E2_1428 0.57 -0.9
Or see this region's nucleotide sequence