Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1317 and Pf6N2E2_1318 are separated by 311 nucleotides Pf6N2E2_1318 and Pf6N2E2_1319 are separated by 182 nucleotides Pf6N2E2_1319 and Pf6N2E2_1320 are separated by 51 nucleotides
Pf6N2E2_1317 - P-hydroxyphenylacetate hydroxylase C2:oxygenase component, at 1,550,723 to 1,551,892
_1317
Pf6N2E2_1318 - FIG00553873: hypothetical protein, at 1,552,204 to 1,553,502
_1318
Pf6N2E2_1319 - FIG074102: hypothetical protein, at 1,553,685 to 1,554,050
_1319
Pf6N2E2_1320 - Putative response regulator, at 1,554,102 to 1,554,761
_1320
Position (kb)
1552
1553
1554 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1551.411 kb on + strand, within Pf6N2E2_1317 at 1551.735 kb on + strand, within Pf6N2E2_1317 at 1551.906 kb on + strand at 1551.907 kb on - strand at 1552.131 kb on - strand at 1552.133 kb on + strand at 1552.228 kb on + strand at 1552.228 kb on + strand at 1552.229 kb on - strand at 1552.229 kb on - strand at 1552.229 kb on - strand at 1552.231 kb on + strand at 1552.249 kb on + strand at 1552.249 kb on + strand at 1552.428 kb on + strand, within Pf6N2E2_1318 at 1552.549 kb on + strand, within Pf6N2E2_1318 at 1552.585 kb on + strand, within Pf6N2E2_1318 at 1552.586 kb on - strand, within Pf6N2E2_1318 at 1552.653 kb on + strand, within Pf6N2E2_1318 at 1552.717 kb on + strand, within Pf6N2E2_1318 at 1552.969 kb on + strand, within Pf6N2E2_1318 at 1553.041 kb on + strand, within Pf6N2E2_1318 at 1553.041 kb on + strand, within Pf6N2E2_1318 at 1553.042 kb on - strand, within Pf6N2E2_1318 at 1553.042 kb on - strand, within Pf6N2E2_1318 at 1553.069 kb on - strand, within Pf6N2E2_1318 at 1553.069 kb on - strand, within Pf6N2E2_1318 at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.839 kb on - strand, within Pf6N2E2_1319 at 1553.874 kb on + strand, within Pf6N2E2_1319 at 1553.874 kb on + strand, within Pf6N2E2_1319 at 1553.874 kb on + strand, within Pf6N2E2_1319 at 1553.952 kb on + strand, within Pf6N2E2_1319 at 1554.033 kb on + strand at 1554.033 kb on + strand at 1554.153 kb on - strand at 1554.187 kb on - strand, within Pf6N2E2_1320 at 1554.187 kb on - strand, within Pf6N2E2_1320 at 1554.258 kb on + strand, within Pf6N2E2_1320 at 1554.258 kb on + strand, within Pf6N2E2_1320 at 1554.271 kb on - strand, within Pf6N2E2_1320 at 1554.379 kb on - strand, within Pf6N2E2_1320
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 1,551,411 + Pf6N2E2_1317 0.59 -0.8 1,551,735 + Pf6N2E2_1317 0.86 -0.6 1,551,906 + -0.1 1,551,907 - -0.0 1,552,131 - +0.6 1,552,133 + -1.8 1,552,228 + +0.9 1,552,228 + +1.4 1,552,229 - -1.0 1,552,229 - +0.2 1,552,229 - -0.5 1,552,231 + -1.7 1,552,249 + +0.2 1,552,249 + +1.0 1,552,428 + Pf6N2E2_1318 0.17 -0.4 1,552,549 + Pf6N2E2_1318 0.27 -1.5 1,552,585 + Pf6N2E2_1318 0.29 -1.6 1,552,586 - Pf6N2E2_1318 0.29 +1.8 1,552,653 + Pf6N2E2_1318 0.35 -1.4 1,552,717 + Pf6N2E2_1318 0.39 +0.4 1,552,969 + Pf6N2E2_1318 0.59 +0.7 1,553,041 + Pf6N2E2_1318 0.64 -0.1 1,553,041 + Pf6N2E2_1318 0.64 -1.5 1,553,042 - Pf6N2E2_1318 0.65 -0.8 1,553,042 - Pf6N2E2_1318 0.65 +3.0 1,553,069 - Pf6N2E2_1318 0.67 +1.2 1,553,069 - Pf6N2E2_1318 0.67 -1.0 1,553,519 + -0.2 1,553,519 + -0.0 1,553,519 + -1.1 1,553,519 + -0.7 1,553,519 + -0.2 1,553,520 - +0.8 1,553,520 - -0.0 1,553,520 - -1.4 1,553,520 - -0.4 1,553,520 - +2.2 1,553,520 - -0.5 1,553,839 - Pf6N2E2_1319 0.42 +0.4 1,553,874 + Pf6N2E2_1319 0.52 -0.9 1,553,874 + Pf6N2E2_1319 0.52 -1.0 1,553,874 + Pf6N2E2_1319 0.52 -1.4 1,553,952 + Pf6N2E2_1319 0.73 +0.1 1,554,033 + +0.4 1,554,033 + +0.6 1,554,153 - +0.9 1,554,187 - Pf6N2E2_1320 0.13 +0.1 1,554,187 - Pf6N2E2_1320 0.13 +0.4 1,554,258 + Pf6N2E2_1320 0.24 -1.9 1,554,258 + Pf6N2E2_1320 0.24 +1.2 1,554,271 - Pf6N2E2_1320 0.26 +0.1 1,554,379 - Pf6N2E2_1320 0.42 +0.6
Or see this region's nucleotide sequence