Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1139 and Pf6N2E2_1140 are separated by 33 nucleotides Pf6N2E2_1140 and Pf6N2E2_1141 are separated by 1 nucleotides Pf6N2E2_1141 and Pf6N2E2_1142 are separated by 49 nucleotides
Pf6N2E2_1139 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 1,335,759 to 1,336,730
_1139
Pf6N2E2_1140 - Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-), at 1,336,764 to 1,337,507
_1140
Pf6N2E2_1141 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 1,337,509 to 1,337,802
_1141
Pf6N2E2_1142 - Polymyxin resistance protein ArnT, undecaprenyl phosphate-alpha-L-Ara4N transferase; Melittin resistance protein PqaB, at 1,337,852 to 1,339,327
_1142
Position (kb)
1336
1337
1338 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1335.865 kb on - strand, within Pf6N2E2_1139 at 1335.867 kb on + strand, within Pf6N2E2_1139 at 1335.867 kb on + strand, within Pf6N2E2_1139 at 1335.868 kb on - strand, within Pf6N2E2_1139 at 1335.868 kb on - strand, within Pf6N2E2_1139 at 1336.053 kb on + strand, within Pf6N2E2_1139 at 1336.054 kb on - strand, within Pf6N2E2_1139 at 1336.072 kb on + strand, within Pf6N2E2_1139 at 1336.072 kb on + strand, within Pf6N2E2_1139 at 1336.073 kb on - strand, within Pf6N2E2_1139 at 1336.174 kb on - strand, within Pf6N2E2_1139 at 1336.266 kb on + strand, within Pf6N2E2_1139 at 1336.305 kb on + strand, within Pf6N2E2_1139 at 1336.305 kb on + strand, within Pf6N2E2_1139 at 1336.306 kb on - strand, within Pf6N2E2_1139 at 1336.306 kb on - strand, within Pf6N2E2_1139 at 1336.416 kb on + strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.418 kb on + strand, within Pf6N2E2_1139 at 1336.497 kb on + strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.707 kb on + strand at 1336.716 kb on + strand at 1336.719 kb on + strand at 1336.720 kb on - strand at 1336.779 kb on + strand at 1336.779 kb on + strand at 1336.780 kb on - strand at 1336.780 kb on - strand at 1336.780 kb on - strand at 1336.783 kb on + strand at 1336.783 kb on + strand at 1336.784 kb on - strand at 1336.817 kb on + strand at 1336.817 kb on + strand at 1336.921 kb on + strand, within Pf6N2E2_1140 at 1336.922 kb on - strand, within Pf6N2E2_1140 at 1336.922 kb on - strand, within Pf6N2E2_1140 at 1337.001 kb on + strand, within Pf6N2E2_1140 at 1337.590 kb on + strand, within Pf6N2E2_1141 at 1337.600 kb on - strand, within Pf6N2E2_1141 at 1337.600 kb on - strand, within Pf6N2E2_1141 at 1337.671 kb on + strand, within Pf6N2E2_1141 at 1337.671 kb on + strand, within Pf6N2E2_1141 at 1337.672 kb on - strand, within Pf6N2E2_1141 at 1337.856 kb on + strand at 1337.856 kb on + strand at 1337.856 kb on + strand at 1337.993 kb on + strand at 1338.071 kb on + strand, within Pf6N2E2_1142 at 1338.072 kb on - strand, within Pf6N2E2_1142 at 1338.072 kb on - strand, within Pf6N2E2_1142 at 1338.116 kb on + strand, within Pf6N2E2_1142 at 1338.116 kb on + strand, within Pf6N2E2_1142 at 1338.194 kb on + strand, within Pf6N2E2_1142 at 1338.194 kb on + strand, within Pf6N2E2_1142 at 1338.194 kb on + strand, within Pf6N2E2_1142 at 1338.257 kb on + strand, within Pf6N2E2_1142 at 1338.257 kb on + strand, within Pf6N2E2_1142 at 1338.257 kb on + strand, within Pf6N2E2_1142 at 1338.258 kb on - strand, within Pf6N2E2_1142 at 1338.258 kb on - strand, within Pf6N2E2_1142 at 1338.258 kb on - strand, within Pf6N2E2_1142 at 1338.302 kb on + strand, within Pf6N2E2_1142 at 1338.303 kb on - strand, within Pf6N2E2_1142 at 1338.305 kb on + strand, within Pf6N2E2_1142 at 1338.305 kb on + strand, within Pf6N2E2_1142 at 1338.306 kb on - strand, within Pf6N2E2_1142 at 1338.306 kb on - strand, within Pf6N2E2_1142 at 1338.369 kb on + strand, within Pf6N2E2_1142 at 1338.389 kb on + strand, within Pf6N2E2_1142 at 1338.389 kb on + strand, within Pf6N2E2_1142
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 1,335,865 - Pf6N2E2_1139 0.11 -0.8 1,335,867 + Pf6N2E2_1139 0.11 +1.2 1,335,867 + Pf6N2E2_1139 0.11 -1.1 1,335,868 - Pf6N2E2_1139 0.11 -0.7 1,335,868 - Pf6N2E2_1139 0.11 -0.1 1,336,053 + Pf6N2E2_1139 0.30 +0.2 1,336,054 - Pf6N2E2_1139 0.30 -0.1 1,336,072 + Pf6N2E2_1139 0.32 +0.8 1,336,072 + Pf6N2E2_1139 0.32 +1.4 1,336,073 - Pf6N2E2_1139 0.32 -2.7 1,336,174 - Pf6N2E2_1139 0.43 -2.5 1,336,266 + Pf6N2E2_1139 0.52 +1.6 1,336,305 + Pf6N2E2_1139 0.56 +0.3 1,336,305 + Pf6N2E2_1139 0.56 -1.1 1,336,306 - Pf6N2E2_1139 0.56 -0.1 1,336,306 - Pf6N2E2_1139 0.56 -0.3 1,336,416 + Pf6N2E2_1139 0.68 +0.1 1,336,417 - Pf6N2E2_1139 0.68 +0.3 1,336,417 - Pf6N2E2_1139 0.68 +0.7 1,336,417 - Pf6N2E2_1139 0.68 -0.2 1,336,417 - Pf6N2E2_1139 0.68 +0.9 1,336,417 - Pf6N2E2_1139 0.68 +0.1 1,336,418 + Pf6N2E2_1139 0.68 -0.7 1,336,497 + Pf6N2E2_1139 0.76 +1.6 1,336,498 - Pf6N2E2_1139 0.76 +1.5 1,336,498 - Pf6N2E2_1139 0.76 +0.3 1,336,498 - Pf6N2E2_1139 0.76 +0.3 1,336,498 - Pf6N2E2_1139 0.76 +0.7 1,336,707 + +1.7 1,336,716 + +0.4 1,336,719 + +0.1 1,336,720 - +1.0 1,336,779 + +0.7 1,336,779 + -0.3 1,336,780 - +0.3 1,336,780 - -1.9 1,336,780 - +0.2 1,336,783 + -0.3 1,336,783 + -1.8 1,336,784 - +0.9 1,336,817 + -1.3 1,336,817 + -0.3 1,336,921 + Pf6N2E2_1140 0.21 -0.7 1,336,922 - Pf6N2E2_1140 0.21 -0.6 1,336,922 - Pf6N2E2_1140 0.21 +0.4 1,337,001 + Pf6N2E2_1140 0.32 -1.1 1,337,590 + Pf6N2E2_1141 0.28 +1.0 1,337,600 - Pf6N2E2_1141 0.31 +2.0 1,337,600 - Pf6N2E2_1141 0.31 +1.8 1,337,671 + Pf6N2E2_1141 0.55 -0.1 1,337,671 + Pf6N2E2_1141 0.55 +0.2 1,337,672 - Pf6N2E2_1141 0.55 -0.0 1,337,856 + -1.8 1,337,856 + -0.1 1,337,856 + +0.0 1,337,993 + -0.3 1,338,071 + Pf6N2E2_1142 0.15 -0.2 1,338,072 - Pf6N2E2_1142 0.15 +0.3 1,338,072 - Pf6N2E2_1142 0.15 -1.3 1,338,116 + Pf6N2E2_1142 0.18 +0.5 1,338,116 + Pf6N2E2_1142 0.18 +2.1 1,338,194 + Pf6N2E2_1142 0.23 -1.3 1,338,194 + Pf6N2E2_1142 0.23 -0.2 1,338,194 + Pf6N2E2_1142 0.23 +0.8 1,338,257 + Pf6N2E2_1142 0.27 -0.8 1,338,257 + Pf6N2E2_1142 0.27 -0.8 1,338,257 + Pf6N2E2_1142 0.27 -0.4 1,338,258 - Pf6N2E2_1142 0.28 +0.7 1,338,258 - Pf6N2E2_1142 0.28 -0.7 1,338,258 - Pf6N2E2_1142 0.28 -2.1 1,338,302 + Pf6N2E2_1142 0.30 +0.1 1,338,303 - Pf6N2E2_1142 0.31 +0.5 1,338,305 + Pf6N2E2_1142 0.31 -0.3 1,338,305 + Pf6N2E2_1142 0.31 -0.3 1,338,306 - Pf6N2E2_1142 0.31 -0.0 1,338,306 - Pf6N2E2_1142 0.31 +0.4 1,338,369 + Pf6N2E2_1142 0.35 -0.5 1,338,389 + Pf6N2E2_1142 0.36 +1.2 1,338,389 + Pf6N2E2_1142 0.36 +1.7
Or see this region's nucleotide sequence